UniProtKB - Q9XDB4 (SYI_FERPD)
Protein
Isoleucine--tRNA ligase
Gene
ileS
Organism
Fervidobacterium pennivorans (strain DSM 9078 / Ven5)
Status
Functioni
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation
Catalytic activityi
- EC:6.1.1.5UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 549 | Aminoacyl-adenylateUniRule annotation | 1 | |
Binding sitei | 593 | ATPUniRule annotation | 1 | |
Metal bindingi | 880 | ZincUniRule annotation | 1 | |
Metal bindingi | 883 | ZincUniRule annotation | 1 | |
Metal bindingi | 900 | ZincUniRule annotation | 1 | |
Metal bindingi | 903 | ZincUniRule annotation | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- isoleucine-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: InterPro
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- isoleucyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | FPEN771875:G1H1H-1748-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)Alternative name(s): Isoleucyl-tRNA synthetaseUniRule annotation Short name: IleRSUniRule annotation |
Gene namesi | Name:ileSUniRule annotation Ordered Locus Names:Ferpe_1791 |
Organismi | Fervidobacterium pennivorans (strain DSM 9078 / Ven5) |
Taxonomic identifieri | 771875 [NCBI] |
Taxonomic lineagei | Bacteria › Thermotogae › Thermotogales › Fervidobacteriaceae › Fervidobacterium › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098387 | 1 – 912 | Isoleucine--tRNA ligaseAdd BLAST | 912 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
STRINGi | 771875.Ferpe_1791 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 57 – 67 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 590 – 594 | 'KMSKS' region | 5 |
Domaini
IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
HOGENOMi | CLU_001493_7_0_0 |
OMAi | HLGTAWN |
OrthoDBi | 32262at2 |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR010663, Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF06827, zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9XDB4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDYKATLNLP QTNFQMKANL VNKEPEMLKF WEEKEIYKKT LETRANAPTY
60 70 80 90 100
LLHDGPPYAN GDIHLGTAMN KILKDFVTRY KTMRGYRVPF VPGWDTHGLP
110 120 130 140 150
IEHRVTTSLG EEAKKKSPAE IRKLCKEFAL KYVDIQREEF KRLGVKGDWE
160 170 180 190 200
HPYITLDPDY EYHILDVFKT LVENGNVYRG NKPVYWCPTC RTALAEAEIE
210 220 230 240 250
YHDHESPSIY VKFQMVDKPD TYIVIWTTTP WTIPANVAIA LHPDYTYVKI
260 270 280 290 300
KVDEEYWIVA EGLLQKFAAD VGIDFEVVEK FVGKELEGKL TKHPLYDRTS
310 320 330 340 350
VVVLADYVTL EDGTGCVHTA PGHGEEDYQT GLKYNLPVLS PVDDEGRFTK
360 370 380 390 400
EAGKYEGLKI WDANKVIVED LKNNGSLIKA GKISHSYPHC WRCKGPVMFR
410 420 430 440 450
ATPQWFISVD KNNLRGKVLE EIKKVKWYPA WGETRITAMV QERPDWTISR
460 470 480 490 500
QRVWGVPIPA VKCKDCGEVT LEPKVIEHFA NIVKEKGTDA WFELDVNELI
510 520 530 540 550
PADFKCPACG SKNFEKTHDT LDVWIDSGCS WEAVIRSKGE RFPVDLYLEG
560 570 580 590 600
DDQHRGWFQS SIFLSTAKAG TAPFKAVVTH GFIKDEQGRK MSKSLGNVID
610 620 630 640 650
PMEIVNKYGA DILRLWVAST DFFDNIRVGK NIIEQQVEVY RKLRNTLRYL
660 670 680 690 700
LSNLYDFTEA DLLPYEKLLP LDKWALGRLQ KFIEQITQYY EGFEYSKVYN
710 720 730 740 750
ATVKYCTTEL SAVYLDILKD RLYVEAKDSI YRRSAQTALH YILEALIKIL
760 770 780 790 800
APIIPFTAEE AYQESHLKRY ESVHLEYWPE VRKEFIDEAL LEEFDHLLLI
810 820 830 840 850
RDDVLKALEN ARASDIIGHS LDAHVIIEAK NEELKNLLRK YESLLEEFFI
860 870 880 890 900
VSKVTLSENI SGLNGQFANV LVQRAEGQKC QRCWKYHPDT GKDLEHPETC
910
PRCSAVLRGE RK
Sequence cautioni
The sequence AAD30388 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 525 | I → M in AAD30388 (PubMed:10224005).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF096862 Genomic DNA Translation: AAD30388.1 Different initiation. CP003260 Genomic DNA Translation: AFG35844.1 |
RefSeqi | WP_014452273.1, NC_017095.1 |
Genome annotation databases
EnsemblBacteriai | AFG35844; AFG35844; Ferpe_1791 |
KEGGi | fpe:Ferpe_1791 |
PATRICi | fig|771875.3.peg.1818 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF096862 Genomic DNA Translation: AAD30388.1 Different initiation. CP003260 Genomic DNA Translation: AFG35844.1 |
RefSeqi | WP_014452273.1, NC_017095.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 771875.Ferpe_1791 |
Genome annotation databases
EnsemblBacteriai | AFG35844; AFG35844; Ferpe_1791 |
KEGGi | fpe:Ferpe_1791 |
PATRICi | fig|771875.3.peg.1818 |
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
HOGENOMi | CLU_001493_7_0_0 |
OMAi | HLGTAWN |
OrthoDBi | 32262at2 |
Enzyme and pathway databases
BioCyci | FPEN771875:G1H1H-1748-MONOMER |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR010663, Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF06827, zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYI_FERPD | |
Accessioni | Q9XDB4Primary (citable) accession number: Q9XDB4 Secondary accession number(s): H9UEA1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | September 5, 2012 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families