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Protein

1-deoxy-D-xylulose 5-phosphate reductoisomerase

Gene

dxr

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).

Catalytic activityi

2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH.UniRule annotation

Cofactori

Mg2+, Mn2+, Co2+Note: Divalent cation. Prefers Mg2+, Mn2+ or Co2+.

Activity regulationi

Inhibited by fosmidomycin.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. Bifunctional enzyme IspD/IspF (ispDF), Bifunctional enzyme IspD/IspF (ispDF)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. Bifunctional enzyme IspD/IspF (ispDF), Bifunctional enzyme IspD/IspF (ispDF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125SubstrateUniRule annotation1
Metal bindingi150Divalent metal cationUniRule annotation1
Metal bindingi152Divalent metal cationUniRule annotation1
Binding sitei152SubstrateUniRule annotation1
Binding sitei176SubstrateUniRule annotation1
Binding sitei199SubstrateUniRule annotation1
Metal bindingi221Divalent metal cationUniRule annotation1
Binding sitei221SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 39NADPUniRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
LigandCobalt, Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciZMOB264203:G1FZK-1039-MONOMER
UniPathwayi
UPA00056;UER00092

Names & Taxonomyi

Protein namesi
Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomeraseUniRule annotation (EC:1.1.1.267UniRule annotation)
Short name:
DXP reductoisomeraseUniRule annotation
Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomeraseUniRule annotation
2-C-methyl-D-erythritol 4-phosphate synthaseUniRule annotation
Gene namesi
Name:dxrUniRule annotation
Ordered Locus Names:ZMO1150
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
Proteomesi
  • UP000001173 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001637471 – 3881-deoxy-D-xylulose 5-phosphate reductoisomeraseAdd BLAST388

Proteomic databases

PRIDEiQ9X5F2

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9X5F2
SMRiQ9X5F2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X5F2

Family & Domainsi

Sequence similaritiesi

Belongs to the DXR family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000007220
KOiK00099
OMAiAHPNWVM

Family and domain databases

HAMAPiMF_00183 DXP_reductoisom, 1 hit
InterProiView protein in InterPro
IPR003821 DXP_reductoisomerase
IPR013644 DXP_reductoisomerase_C
IPR013512 DXP_reductoisomerase_N
IPR026877 DXPR_C
IPR036169 DXPR_C_sf
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR30525 PTHR30525, 1 hit
PfamiView protein in Pfam
PF08436 DXP_redisom_C, 1 hit
PF02670 DXP_reductoisom, 1 hit
PF13288 DXPR_C, 1 hit
PIRSFiPIRSF006205 Dxp_reductismrs, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF69055 SSF69055, 1 hit
TIGRFAMsiTIGR00243 Dxr, 1 hit

Sequencei

Sequence statusi: Complete.

Q9X5F2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQPRTVTVL GATGSIGHST LDLIERNLDR YQVIALTANR NVKDLADAAK
60 70 80 90 100
RTNAKRAVIA DPSLYNDLKE ALAGSSVEAA AGADALVEAA MMGADWTMAA
110 120 130 140 150
IIGCAGLKAT LAAIRKGKTV ALANKESLVS AGGLMIDAVR EHGTTLLPVD
160 170 180 190 200
SEHNAIFQCF PHHNRDYVRR IIITASGGPF RTTSLAEMAT VTPERAVQHP
210 220 230 240 250
NWSMGAKISI DSATMMNKGL ELIEAYHLFQ IPLEKFEILV HPQSVIHSMV
260 270 280 290 300
EYLDGSILAQ IGSPDMRTPI GHTLAWPKRM ETPAESLDFT KLRQMDFEAP
310 320 330 340 350
DYERFPALTL AMESIKSGGA RPAVMNAANE IAVAAFLDKK IGFLDIAKIV
360 370 380
EKTLDHYTPA TPSSLEDVFA IDNEARIQAA ALMESLPA
Length:388
Mass (Da):41,858
Last modified:February 15, 2005 - v3
Checksum:i373EB372AA325AAF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti226Y → F in CAB60758 (PubMed:11004410).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250714 Genomic DNA Translation: CAB60758.1
AF124757 Genomic DNA Translation: AAD29659.1
AE008692 Genomic DNA Translation: AAV89774.1
RefSeqiWP_011240977.1, NZ_CP023715.1

Genome annotation databases

EnsemblBacteriaiAAV89774; AAV89774; ZMO1150
GeneIDi33074329
KEGGizmo:ZMO1150

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250714 Genomic DNA Translation: CAB60758.1
AF124757 Genomic DNA Translation: AAD29659.1
AE008692 Genomic DNA Translation: AAV89774.1
RefSeqiWP_011240977.1, NZ_CP023715.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R0KX-ray1.91A/B/C/D1-388[»]
1R0LX-ray2.70A/B/C/D1-388[»]
ProteinModelPortaliQ9X5F2
SMRiQ9X5F2
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9X5F2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV89774; AAV89774; ZMO1150
GeneIDi33074329
KEGGizmo:ZMO1150

Phylogenomic databases

HOGENOMiHOG000007220
KOiK00099
OMAiAHPNWVM

Enzyme and pathway databases

UniPathwayi
UPA00056;UER00092

BioCyciZMOB264203:G1FZK-1039-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ9X5F2

Family and domain databases

HAMAPiMF_00183 DXP_reductoisom, 1 hit
InterProiView protein in InterPro
IPR003821 DXP_reductoisomerase
IPR013644 DXP_reductoisomerase_C
IPR013512 DXP_reductoisomerase_N
IPR026877 DXPR_C
IPR036169 DXPR_C_sf
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR30525 PTHR30525, 1 hit
PfamiView protein in Pfam
PF08436 DXP_redisom_C, 1 hit
PF02670 DXP_reductoisom, 1 hit
PF13288 DXPR_C, 1 hit
PIRSFiPIRSF006205 Dxp_reductismrs, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF69055 SSF69055, 1 hit
TIGRFAMsiTIGR00243 Dxr, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDXR_ZYMMO
AccessioniPrimary (citable) accession number: Q9X5F2
Secondary accession number(s): Q5NND6, Q9RIA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 15, 2005
Last modified: November 7, 2018
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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