UniProtKB - Q9X4Y0 (AGAL_RHIME)
Protein
Alpha-galactosidase
Gene
melA
Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Functioni
Catalytic activityi
- Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. EC:3.2.1.22
Cofactori
Protein has several cofactor binding sites:- Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity
- NAD+By similarityNote: Binds 1 NAD+ per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 150 | SubstrateBy similarity | 1 | |
Metal bindingi | 171 | ManganeseBy similarity | 1 | |
Active sitei | 172 | Proton donorBy similarity | 1 | |
Metal bindingi | 201 | ManganeseBy similarity | 1 | |
Active sitei | 258 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 4 – 70 | NADBy similarityAdd BLAST | 67 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
- raffinose alpha-galactosidase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Manganese, Metal-binding, NAD |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:melA Ordered Locus Names:RB1568 ORF Names:SMb21648 |
Encoded oni | Plasmid pSymB (megaplasmid 2) |
Organismi | Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) |
Taxonomic identifieri | 266834 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169853 | 1 – 490 | Alpha-galactosidaseAdd BLAST | 490 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 266834.SM_b21648 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 4 family.Curated
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
HOGENOMi | CLU_045951_1_1_5 |
OMAi | NEHASHI |
Family and domain databases
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9X4Y0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASFKIAIIG AGSIGFTKKL FTDILSVPEL RDVEFALTDL SEHNLAMIKS
60 70 80 90 100
ILDRIVEANE LPTRVTATTD RRKALEGARY IISCVRVGGL EAYADDIRIP
110 120 130 140 150
LKYGVDQCVG DTICAGGILY GQRNIPVILD FCKDIREVAE PGAKFLNYAN
160 170 180 190 200
PMAMNTWAAI EYGKVDTVGL CHGVQHGAEQ IAEILGARDG ELDYICSGIN
210 220 230 240 250
HQTWFVDVRL NGRKVGKDEL VAAFEAHPVF SKQEKLRIDV LKRFGVYSTE
260 270 280 290 300
SNGHLSEYLP WYRKRPDEIS RWIDMSDWIH GETGGYLRYS TETRNWFETE
310 320 330 340 350
YPRFLEEAGR PLETIRRSNE HASRILEALE TGRVYRGHFN VKNNGVITNL
360 370 380 390 400
PADAIIESPG FVDRFGINMV AGITLPEACA ATCISSVNVQ RMSVHAAITG
410 420 430 440 450
DIDLLKLAVL HDPLVGAICT PEEVWQMVDE MVVAQAKWLP QYAHAIDAAK
460 470 480 490
ERLARATVAT REWKGAARRE VRSIEEIRAE KEAAKLRAAG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF119834 Genomic DNA Translation: AAD26273.1 AL591985 Genomic DNA Translation: CAC49967.1 |
PIRi | G96037 |
RefSeqi | NP_438107.1, NC_003078.1 WP_003527988.1, NC_003078.1 |
Genome annotation databases
EnsemblBacteriai | CAC49967; CAC49967; SM_b21648 |
KEGGi | sme:SM_b21648 |
PATRICi | fig|266834.11.peg.6494 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF119834 Genomic DNA Translation: AAD26273.1 AL591985 Genomic DNA Translation: CAC49967.1 |
PIRi | G96037 |
RefSeqi | NP_438107.1, NC_003078.1 WP_003527988.1, NC_003078.1 |
3D structure databases
SMRi | Q9X4Y0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 266834.SM_b21648 |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Genome annotation databases
EnsemblBacteriai | CAC49967; CAC49967; SM_b21648 |
KEGGi | sme:SM_b21648 |
PATRICi | fig|266834.11.peg.6494 |
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
HOGENOMi | CLU_045951_1_1_5 |
OMAi | NEHASHI |
Family and domain databases
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AGAL_RHIME | |
Accessioni | Q9X4Y0Primary (citable) accession number: Q9X4Y0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Plasmid, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families