UniProtKB - Q9X4K2 (AZOR_GEOSE)
Protein
FMN-dependent NADH:quinone oxidoreductase
Gene
azoR
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Functioni
Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones.UniRule annotation
Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines.UniRule annotation
Caution
Was originally (Ref. 1) thought to be a thermostable diaphorase.Curated
Catalytic activityi
- anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H+ + 2 NADHUniRule annotationEC:1.7.1.17UniRule annotation
Cofactori
FMNUniRule annotationNote: Binds 1 FMN per subunit.UniRule annotation
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 17 – 19 | FMNUniRule annotation | 3 | |
Nucleotide bindingi | 102 – 105 | FMNUniRule annotation | 4 |
GO - Molecular functioni
- electron transfer activity Source: UniProtKB-UniRule
- FMN binding Source: UniProtKB-UniRule
- oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor Source: UniProtKB-UniRule
- oxidoreductase activity, acting on other nitrogenous compounds as donors Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Flavoprotein, FMN, NAD |
Enzyme and pathway databases
BRENDAi | 1.7.1.6, 623 |
Names & Taxonomyi
Protein namesi | Recommended name: FMN-dependent NADH:quinone oxidoreductaseUniRule annotation (EC:1.6.5.-UniRule annotation)Alternative name(s): Azo-dye reductaseUniRule annotation FMN-dependent NADH-azo compound oxidoreductaseUniRule annotation FMN-dependent NADH-azoreductaseUniRule annotation (EC:1.7.1.17UniRule annotation) |
Gene namesi | Name:azoRUniRule annotation |
Organismi | Geobacillus stearothermophilus (Bacillus stearothermophilus) |
Taxonomic identifieri | 1422 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166327 | 1 – 211 | FMN-dependent NADH:quinone oxidoreductaseAdd BLAST | 211 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationFamily & Domainsi
Sequence similaritiesi
Belongs to the azoreductase type 1 family.UniRule annotation
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
HAMAPi | MF_01216, Azoreductase_type1, 1 hit |
InterProi | View protein in InterPro IPR003680, Flavodoxin_fold IPR029039, Flavoprotein-like_sf IPR023048, NADH-azoreductase_FMN-depdnt |
Pfami | View protein in Pfam PF02525, Flavodoxin_2, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9X4K2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTNVLYITAH PHDDTQSYSM AVGKAFIDTY KQVHPDHEVI HLDLYKEYIP
60 70 80 90 100
EIDVDVFSGW GKLRSGKSFE ELSDEEKAKV GRMNELCEQF ISADKYVFVT
110 120 130 140 150
PMWNFSFPPV LKAYIDAVAV AGKTFKYTEQ GPVGLLTDKK ALHIQARGGF
160 170 180 190 200
YSEGPAAEME MGHRYLSVIM QFFGVPSFEG LFVEGHAAVP EKAEEIKANA
210
IARAKDLAHT F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF112858 Genomic DNA Translation: AAD24436.1 |
RefSeqi | WP_033015399.1, NZ_RCTG01000072.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF112858 Genomic DNA Translation: AAD24436.1 |
RefSeqi | WP_033015399.1, NZ_RCTG01000072.1 |
3D structure databases
SMRi | Q9X4K2 |
ModBasei | Search... |
Enzyme and pathway databases
BRENDAi | 1.7.1.6, 623 |
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
HAMAPi | MF_01216, Azoreductase_type1, 1 hit |
InterProi | View protein in InterPro IPR003680, Flavodoxin_fold IPR029039, Flavoprotein-like_sf IPR023048, NADH-azoreductase_FMN-depdnt |
Pfami | View protein in Pfam PF02525, Flavodoxin_2, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AZOR_GEOSE | |
Accessioni | Q9X4K2Primary (citable) accession number: Q9X4K2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 26, 2002 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 60 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |