UniProtKB - Q9X2E6 (RIBBA_THEMA)
Protein
Riboflavin biosynthesis protein RibBA
Gene
ribBA
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Functioni
Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotation
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotation
Catalytic activityi
- EC:4.1.99.12UniRule annotation
- GTP + 3 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate + formate + 2 H+UniRule annotationEC:3.5.4.25UniRule annotation
Cofactori
Protein has several cofactor binding sites:- Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation
- Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
: riboflavin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Riboflavin biosynthesis protein RibBA (ribBA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.
Pathwayi: riboflavin biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.UniRule annotationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- Riboflavin biosynthesis protein RibBA (ribBA), Riboflavin biosynthesis protein RibBA (ribBA)
- no protein annotated in this organism
- Riboflavin biosynthesis protein RibD (Tmari_1838), Riboflavin biosynthesis protein RibD (TM_1828)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 22 | Magnesium or manganese 1UniRule annotation | 1 | |
Metal bindingi | 22 | Magnesium or manganese 2UniRule annotation | 1 | |
Binding sitei | 26 | D-ribulose 5-phosphateUniRule annotation | 1 | |
Sitei | 113 | Essential for DHBP synthase activityUniRule annotation | 1 | |
Metal bindingi | 128 | Magnesium or manganese 2UniRule annotation | 1 | |
Binding sitei | 149 | D-ribulose 5-phosphateUniRule annotation | 1 | |
Sitei | 149 | Essential for DHBP synthase activityUniRule annotation | 1 | |
Metal bindingi | 240 | Zinc; catalyticUniRule annotation | 1 | |
Metal bindingi | 251 | Zinc; catalyticUniRule annotation | 1 | |
Metal bindingi | 253 | Zinc; catalyticUniRule annotation | 1 | |
Binding sitei | 256 | GTPUniRule annotation | 1 | |
Binding sitei | 299 | GTPUniRule annotation | 1 | |
Active sitei | 311 | Proton acceptor; for GTP cyclohydrolase activityUniRule annotation | 1 | |
Active sitei | 313 | Nucleophile; for GTP cyclohydrolase activityUniRule annotation | 1 | |
Binding sitei | 334 | GTPUniRule annotation | 1 | |
Binding sitei | 339 | GTPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 235 – 239 | GTPUniRule annotation | 5 | |
Nucleotide bindingi | 277 – 279 | GTPUniRule annotation | 3 |
GO - Molecular functioni
- 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-UniRule
- GTP binding Source: UniProtKB-KW
- GTP cyclohydrolase II activity Source: GO_Central
- magnesium ion binding Source: UniProtKB-UniRule
- manganese ion binding Source: UniProtKB-UniRule
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- riboflavin biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Lyase, Multifunctional enzyme |
Biological process | Riboflavin biosynthesis |
Ligand | GTP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00275;UER00399 UPA00275;UER00400 |
Names & Taxonomyi
Protein namesi | Recommended name: Riboflavin biosynthesis protein RibBAUniRule annotationIncluding the following 2 domains: |
Gene namesi | Name:ribBAUniRule annotation Ordered Locus Names:TM_1826 |
Organismi | Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) |
Taxonomic identifieri | 243274 [NCBI] |
Taxonomic lineagei | Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000151744 | 1 – 388 | Riboflavin biosynthesis protein RibBAAdd BLAST | 388 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 186 | DHBP synthaseAdd BLAST | 186 | |
Regioni | 21 – 22 | D-ribulose 5-phosphate bindingUniRule annotation | 2 | |
Regioni | 125 – 129 | D-ribulose 5-phosphate bindingUniRule annotation | 5 | |
Regioni | 187 – 388 | GTP cyclohydrolase IIAdd BLAST | 202 |
Sequence similaritiesi
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotation
In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0108, Bacteria COG0807, Bacteria |
InParanoidi | Q9X2E6 |
OMAi | ECRGLIC |
OrthoDBi | 900513at2 |
Family and domain databases
CDDi | cd00641, GTP_cyclohydro2, 1 hit |
Gene3Di | 3.40.50.10990, 1 hit |
HAMAPi | MF_00179, RibA, 1 hit MF_01283, RibBA, 1 hit |
InterProi | View protein in InterPro IPR017945, DHBP_synth_RibB-like_a/b_dom IPR000422, DHBP_synthase_RibB IPR032677, GTP_cyclohydro_II IPR000926, RibA IPR036144, RibA-like_sf IPR016299, Riboflavin_synth_RibBA |
Pfami | View protein in Pfam PF00926, DHBP_synthase, 1 hit PF00925, GTP_cyclohydro2, 1 hit |
SUPFAMi | SSF142695, SSF142695, 1 hit SSF55821, SSF55821, 1 hit |
TIGRFAMsi | TIGR00505, ribA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9X2E6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEELREAFEE GKPVILIDRN RENEADFVFP AQLITEDVVS FFVTYGKGLF
60 70 80 90 100
CVTADEEDLL KRGFVKLSSN YGANYFVPVD WGTGTGISAL ERAETCRKLA
110 120 130 140 150
EGRYFHEFRY PGHVTVIGGI GFQRRKGHTE ASLEISELAG FSRHAVIVEI
160 170 180 190 200
LDEKGNSHNL DYVLKLSEKF SLPVLEMDDV WREFVKRKLL MKKKAEATLP
210 220 230 240 250
TDFGVFKVVS FENHLDGKEH FAIVKEPLED PVAVRIHSEC VTGDVLSSLR
260 270 280 290 300
CDCGSQLANF LRYMSAHGGI LIYLRQEGRG IGLSNKIAAY SLQDKGLDTV
310 320 330 340 350
EANRVLGFSE DERDYAPAAQ ILKALGIERV LLFTNNQRKT VGLEKYGIEV
360 370 380
VETKRLYGRV TPHNRFYLST KMKKLGHELE EIFREVNS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD36889.1 |
PIRi | E72207 |
RefSeqi | NP_229623.1, NC_000853.1 WP_004082372.1, NZ_CP011107.1 |
Genome annotation databases
EnsemblBacteriai | AAD36889; AAD36889; TM_1826 |
KEGGi | tma:TM1826 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD36889.1 |
PIRi | E72207 |
RefSeqi | NP_229623.1, NC_000853.1 WP_004082372.1, NZ_CP011107.1 |
3D structure databases
SMRi | Q9X2E6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 243274.THEMA_05065 |
Genome annotation databases
EnsemblBacteriai | AAD36889; AAD36889; TM_1826 |
KEGGi | tma:TM1826 |
Phylogenomic databases
eggNOGi | COG0108, Bacteria COG0807, Bacteria |
InParanoidi | Q9X2E6 |
OMAi | ECRGLIC |
OrthoDBi | 900513at2 |
Enzyme and pathway databases
UniPathwayi | UPA00275;UER00399 UPA00275;UER00400 |
Family and domain databases
CDDi | cd00641, GTP_cyclohydro2, 1 hit |
Gene3Di | 3.40.50.10990, 1 hit |
HAMAPi | MF_00179, RibA, 1 hit MF_01283, RibBA, 1 hit |
InterProi | View protein in InterPro IPR017945, DHBP_synth_RibB-like_a/b_dom IPR000422, DHBP_synthase_RibB IPR032677, GTP_cyclohydro_II IPR000926, RibA IPR036144, RibA-like_sf IPR016299, Riboflavin_synth_RibBA |
Pfami | View protein in Pfam PF00926, DHBP_synthase, 1 hit PF00925, GTP_cyclohydro2, 1 hit |
SUPFAMi | SSF142695, SSF142695, 1 hit SSF55821, SSF55821, 1 hit |
TIGRFAMsi | TIGR00505, ribA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIBBA_THEMA | |
Accessioni | Q9X2E6Primary (citable) accession number: Q9X2E6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families