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UniProtKB - Q9X1T3 (LYSRA_THEMA)
Protein
Lysine racemase
Gene
TM_1597
Organism
Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
Status
Functioni
Catalyzes the interconversion of D-lysine and L-lysine. Has also high activity toward ornithine, and weaker activity toward alanine. Contributes to production of D-lysine and D-alanine for use as peptidoglycan components.
1 PublicationCatalytic activityi
Cofactori
pyridoxal 5'-phosphate1 Publication
Kineticsi
kcat is 693 sec(-1) with L-lysine as substrate. kcat is 669 sec(-1) with D-lysine as substrate. kcat is 537 sec(-1) with L-ornithine as substrate. kcat is 447 sec(-1) with D-ornithine as substrate. kcat is 965 sec(-1) with L-alanine as substrate. kcat is 725 sec(-1) with D-alanine as substrate.1 Publication
- KM=2.14 mM for L-lysine1 Publication
- KM=1.77 mM for D-lysine1 Publication
- KM=1.13 mM for L-ornithine1 Publication
- KM=1.01 mM for D-ornithine1 Publication
- KM=375 mM for L-alanine1 Publication
- KM=282 mM for D-alanine1 Publication
- Vmax=16.9 µmol/sec/mg enzyme with L-lysine as substrate1 Publication
- Vmax=16.3 µmol/sec/mg enzyme with D-lysine as substrate1 Publication
- Vmax=13.1 µmol/sec/mg enzyme with L-ornithine as substrate1 Publication
- Vmax=10.9 µmol/sec/mg enzyme with D-ornithine as substrate1 Publication
- Vmax=23.5 µmol/sec/mg enzyme with L-alanine as substrate1 Publication
- Vmax=17.7 µmol/sec/mg enzyme with D-alanine as substrate1 Publication
pH dependencei
Optimum pH is 9.0-10.0.1 Publication
Temperature dependencei
Optimum temperature is 85-95 degrees Celsius.1 Publication
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
GO - Molecular functioni
- alanine racemase activity Source: GO_Central
- lysine racemase activity Source: UniProtKB-EC
- ornithine racemase activity Source: UniProtKB-EC
- pyridoxal phosphate binding Source: GO_Central
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:TM_1597Imported |
Organismi | Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) |
Taxonomic identifieri | 243274 [NCBI] |
Taxonomic lineagei | Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000448682 | 1 – 354 | Lysine racemaseAdd BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 36 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 243274.THEMA_06265 |
Family & Domainsi
Sequence similaritiesi
Belongs to the alanine racemase family.Curated
Phylogenomic databases
OMAi | GTNLNCL |
OrthoDBi | 859043at2 |
Family and domain databases
Gene3Di | 3.20.20.10, 1 hit |
InterProi | View protein in InterPro IPR001608, Ala_racemase_N IPR029066, PLP-binding_barrel |
Pfami | View protein in Pfam PF01168, Ala_racemase_N, 1 hit |
SUPFAMi | SSF51419, SSF51419, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9X1T3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVYPRLLINL KEIEENARKV VEMASRRGIE IVGVTKVTLG DPRFAETLRK
60 70 80 90 100
AGIGILGESR IRNVLRMKKA GIEGPFMLLR LPMMSELVED VKHFDYIMVS
110 120 130 140 150
DPDVAKKVDE LSREMKRNVK IIYMIDVGDL REGVWFEKAV EEIAQCRGAN
160 170 180 190 200
IVGIGTNFGC YGGIIPTREK FEILLDIKEK LEKNHGFNIE IVSGGNTPAL
210 220 230 240 250
YALENGEIPE GINQLRIGEA IVLGRDITNN RVIDWLSQNT FLIEAEVIEV
260 270 280 290 300
KEKPSVPLGK RGLDVFGRKV DFVDRGIRKR AICALGEQDI DSRGLIPVDK
310 320 330 340 350
GVEVLHASSD HIVLDVTDFG DVKVGDVFRF RMTYSCLLKA MTSPFVEKVY
EPSI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD36664.1 |
PIRi | A72234 |
RefSeqi | NP_229397.1, NC_000853.1 WP_004082040.1, NZ_CP011107.1 |
Genome annotation databases
EnsemblBacteriai | AAD36664; AAD36664; TM_1597 |
KEGGi | tma:TM1597 tmw:THMA_1637 |
PATRICi | fig|243274.18.peg.1213 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD36664.1 |
PIRi | A72234 |
RefSeqi | NP_229397.1, NC_000853.1 WP_004082040.1, NZ_CP011107.1 |
3D structure databases
SMRi | Q9X1T3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 243274.THEMA_06265 |
Genome annotation databases
EnsemblBacteriai | AAD36664; AAD36664; TM_1597 |
KEGGi | tma:TM1597 tmw:THMA_1637 |
PATRICi | fig|243274.18.peg.1213 |
Phylogenomic databases
OMAi | GTNLNCL |
OrthoDBi | 859043at2 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Family and domain databases
Gene3Di | 3.20.20.10, 1 hit |
InterProi | View protein in InterPro IPR001608, Ala_racemase_N IPR029066, PLP-binding_barrel |
Pfami | View protein in Pfam PF01168, Ala_racemase_N, 1 hit |
SUPFAMi | SSF51419, SSF51419, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LYSRA_THEMA | |
Accessioni | Q9X1T3Primary (citable) accession number: Q9X1T3 Secondary accession number(s): G4FFY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 11, 2019 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 88 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families