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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Gene

ispD

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei14Transition state stabilizerUniRule annotation1
Sitei21Transition state stabilizerUniRule annotation1
Sitei149Positions MEP for the nucleophilic attackUniRule annotation1
Sitei201Positions MEP for the nucleophilic attackUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processIsoprene biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseUniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthaseUniRule annotation
MEP cytidylyltransferaseUniRule annotation
Short name:
MCTUniRule annotation
Gene namesi
Name:ispDUniRule annotation
Ordered Locus Names:TM_1393
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB02431 Cytidine-5'-Triphosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000756381 – 2222-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseAdd BLAST222

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1393

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi13 – 15Combined sources3
Helixi21 – 23Combined sources3
Helixi33 – 41Combined sources9
Beta strandi46 – 52Combined sources7
Helixi54 – 56Combined sources3
Helixi57 – 61Combined sources5
Beta strandi69 – 74Combined sources6
Helixi79 – 90Combined sources12
Helixi91 – 93Combined sources3
Beta strandi96 – 102Combined sources7
Helixi110 – 123Combined sources14
Beta strandi124 – 131Combined sources8
Beta strandi134 – 140Combined sources7
Beta strandi143 – 147Combined sources5
Beta strandi152 – 162Combined sources11
Helixi163 – 170Combined sources8
Beta strandi177 – 179Combined sources3
Helixi180 – 184Combined sources5
Turni185 – 187Combined sources3
Beta strandi191 – 194Combined sources4
Helixi197 – 199Combined sources3
Helixi207 – 218Combined sources12

3D structure databases

ProteinModelPortaliQ9X1B3
SMRiQ9X1B3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X1B3

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE5 Bacteria
COG1211 LUCA
InParanoidiQ9X1B3
KOiK00991
OMAiERQHSVY

Family and domain databases

CDDicd02516 CDP-ME_synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_00108 IspD, 1 hit
InterProiView protein in InterPro
IPR001228 IspD
IPR034683 IspD/TarI
IPR018294 ISPD_synthase_CS
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01128 IspD, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR00453 ispD, 1 hit
PROSITEiView protein in PROSITE
PS01295 ISPD, 1 hit

Sequencei

Sequence statusi: Complete.

Q9X1B3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVAILLAAG KGERMSENVP KQFLEIEGRM LFEYPLSTFL KSEAIDGVVI
60 70 80 90 100
VTRREWFEVV EKRVFHEKVL GIVEGGDTRS QSVRSALEFL EKFSPSYVLV
110 120 130 140 150
HDSARPFLRK KHVSEVLRRA RETGAATLAL KNSDALVRVE NDRIEYIPRK
160 170 180 190 200
GVYRILTPQA FSYEILKKAH ENGGEWADDT EPVQKLGVKI ALVEGDPLCF
210 220
KVTFKEDLEL ARIIAREWER IP
Length:222
Mass (Da):25,370
Last modified:November 1, 1999 - v1
Checksum:i51D5E945D078ACEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD36464.1
PIRiB72259
RefSeqiNP_229194.1, NC_000853.1
WP_004081602.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD36464; AAD36464; TM_1393
GeneIDi898083
KEGGitma:TM1393

Similar proteinsi

Entry informationi

Entry nameiISPD_THEMA
AccessioniPrimary (citable) accession number: Q9X1B3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: June 7, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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