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Protein

6-phospho-beta-glucosidase BglT

Gene

bglT

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes cellobiose 6'-phosphate into glucose 6-phosphate (Glc6P) and glucose.

Miscellaneous

Is a retaining glucosidase as it hydrolyzes glycosidic bond with retention of anomeric configuration. Reaction mechanism includes redox steps involving NAD and stabilization of intermediates by Mn2+.

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • NAD+Note: Binds 1 NAD+ per subunit.
  • Mn2+Note: Binds 1 Mn2+ ion per subunit.

Kineticsi

  1. KM=41 µM for p-nitrophenyl-beta-D-glucopyranoside 6-phosphate

    pH dependencei

    Optimum pH is 8.0. Active from pH 6.5 to 10.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei87Substrate1
    Sitei103Increases basicity of active site TyrBy similarity1
    Binding sitei140Substrate1
    Metal bindingi162Manganese1
    Binding sitei163Substrate1
    Metal bindingi192Manganese1
    Active sitei241Proton acceptor1
    Binding sitei261Substrate1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi1 – 64NADAdd BLAST64

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processCarbohydrate metabolism
    LigandManganese, Metal-binding, NAD

    Enzyme and pathway databases

    BRENDAi3.2.1.86 6331
    SABIO-RKiQ9X108

    Protein family/group databases

    CAZyiGH4 Glycoside Hydrolase Family 4

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-beta-glucosidase BglT (EC:3.2.1.86)
    Gene namesi
    Name:bglT
    Ordered Locus Names:TM_1281
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    Proteomesi
    • UP000008183 Componenti: Chromosome

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB02007 alpha-D-glucose 6-phosphate

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001698571 – 4156-phospho-beta-glucosidase BglTAdd BLAST415

    Interactioni

    Subunit structurei

    Homodimer or homotetramer. Exists in a homodimer/homotetramer equilibrium state in solution.

    Protein-protein interaction databases

    STRINGi243274.TM1281

    Structurei

    Secondary structure

    1415
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliQ9X108
    SMRiQ9X108
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9X108

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 4 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105F8D Bacteria
    COG1486 LUCA
    InParanoidiQ9X108
    KOiK01222
    OMAiYYFNREA

    Family and domain databases

    Gene3Di3.90.110.10, 2 hits
    InterProiView protein in InterPro
    IPR019802 GlycHydrolase_4_CS
    IPR001088 Glyco_hydro_4
    IPR022616 Glyco_hydro_4_C
    IPR015955 Lactate_DH/Glyco_Ohase_4_C
    IPR036291 NAD(P)-bd_dom_sf
    PANTHERiPTHR32092 PTHR32092, 1 hit
    PfamiView protein in Pfam
    PF02056 Glyco_hydro_4, 1 hit
    PF11975 Glyco_hydro_4C, 1 hit
    PRINTSiPR00732 GLHYDRLASE4
    SUPFAMiSSF51735 SSF51735, 1 hit
    SSF56327 SSF56327, 1 hit
    PROSITEiView protein in PROSITE
    PS01324 GLYCOSYL_HYDROL_F4, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9X108-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MRIAVIGGGS SYTPELVKGL LDISEDVRID EVIFYDIDEE KQKIVVDFVK
    60 70 80 90 100
    RLVKDRFKVL ISDTFEGAVV DAKYVIFQFR PGGLKGREND EGIPLKYGLI
    110 120 130 140 150
    GQETTGVGGF SAALRAFPIV EEYVDTVRKT SNATIVNFTN PSGHITEFVR
    160 170 180 190 200
    NYLEYEKFIG LCNVPINFIR EIAEMFSARL EDVFLKYYGL NHLSFIEKVF
    210 220 230 240 250
    VKGEDVTEKV FENLKLKLSN IPDEDFPTWF YDSVRLIVNP YLRYYLMEKK
    260 270 280 290 300
    MFKKISTHEL RAREVMKIEK ELFEKYRTAV EIPEELTKRG GSMYSTAAAH
    310 320 330 340 350
    LIRDLETDEG KIHIVNTRNN GSIENLPDDY VLEIPCYVRS GRVHTLSQGK
    360 370 380 390 400
    GDHFALSFIH AVKMYERLTI EAYLKRSKKL ALKALLSHPL GPDVEDAKDL
    410
    LEEILEANRE YVKLG
    Length:415
    Mass (Da):47,627
    Last modified:November 1, 1999 - v1
    Checksum:iFB4E3B358245BFEE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000512 Genomic DNA Translation: AAD36356.1
    PIRiE72273
    RefSeqiNP_229086.1, NC_000853.1
    WP_004079953.1, NZ_CP011107.1

    Genome annotation databases

    EnsemblBacteriaiAAD36356; AAD36356; TM_1281
    GeneIDi898202
    KEGGitma:TM1281

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000512 Genomic DNA Translation: AAD36356.1
    PIRiE72273
    RefSeqiNP_229086.1, NC_000853.1
    WP_004079953.1, NZ_CP011107.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1UP4X-ray2.85A/B/C/D/E/F/G/H1-415[»]
    1UP6X-ray2.55A/B/C/D/E/F/G/H1-415[»]
    1UP7X-ray2.40A/B/C/D/E/F/G/H1-415[»]
    ProteinModelPortaliQ9X108
    SMRiQ9X108
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi243274.TM1281

    Chemistry databases

    DrugBankiDB02007 alpha-D-glucose 6-phosphate

    Protein family/group databases

    CAZyiGH4 Glycoside Hydrolase Family 4

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD36356; AAD36356; TM_1281
    GeneIDi898202
    KEGGitma:TM1281

    Phylogenomic databases

    eggNOGiENOG4105F8D Bacteria
    COG1486 LUCA
    InParanoidiQ9X108
    KOiK01222
    OMAiYYFNREA

    Enzyme and pathway databases

    BRENDAi3.2.1.86 6331
    SABIO-RKiQ9X108

    Miscellaneous databases

    EvolutionaryTraceiQ9X108

    Family and domain databases

    Gene3Di3.90.110.10, 2 hits
    InterProiView protein in InterPro
    IPR019802 GlycHydrolase_4_CS
    IPR001088 Glyco_hydro_4
    IPR022616 Glyco_hydro_4_C
    IPR015955 Lactate_DH/Glyco_Ohase_4_C
    IPR036291 NAD(P)-bd_dom_sf
    PANTHERiPTHR32092 PTHR32092, 1 hit
    PfamiView protein in Pfam
    PF02056 Glyco_hydro_4, 1 hit
    PF11975 Glyco_hydro_4C, 1 hit
    PRINTSiPR00732 GLHYDRLASE4
    SUPFAMiSSF51735 SSF51735, 1 hit
    SSF56327 SSF56327, 1 hit
    PROSITEiView protein in PROSITE
    PS01324 GLYCOSYL_HYDROL_F4, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBGLT_THEMA
    AccessioniPrimary (citable) accession number: Q9X108
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: November 1, 1999
    Last modified: February 28, 2018
    This is version 109 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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