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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (EC:6.3.5.-)
Short name:
Asp/Glu-ADT subunit B
Gene namesi
Name:gatB
Ordered Locus Names:TM_1273
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001488571 – 482Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BAdd BLAST482

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

DIPiDIP-59229N
IntActiQ9X100, 1 interactor
STRINGi243274.TM1273

Structurei

Secondary structure

1482
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9X100
SMRiQ9X100
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X100

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CHT Bacteria
COG0064 LUCA
InParanoidiQ9X100
KOiK02434
OMAiSDGNMQE

Family and domain databases

Gene3Di1.10.10.410, 1 hit
HAMAPiMF_00121 GatB, 1 hit
InterProiView protein in InterPro
IPR004413 Apn/Gln-ADT_bsu
IPR017959 Asn/Gln-tRNA_amidoTrfase_suB/E
IPR006075 Asn/Gln-tRNA_Trfase_suB/E_cat
IPR018027 Asn/Gln_amidotransferase
IPR003789 Asn/Gln_tRNA_amidoTrase-B-like
IPR023168 GatB_Yqey_C
IPR017958 Gln-tRNA_amidoTrfase_suB_CS
IPR014746 Gln_synth/guanido_kin_cat_dom
PANTHERiPTHR11659 PTHR11659, 1 hit
PfamiView protein in Pfam
PF02934 GatB_N, 1 hit
PF02637 GatB_Yqey, 1 hit
SMARTiView protein in SMART
SM00845 GatB_Yqey, 1 hit
SUPFAMiSSF55931 SSF55931, 1 hit
SSF89095 SSF89095, 1 hit
TIGRFAMsiTIGR00133 gatB, 1 hit
PROSITEiView protein in PROSITE
PS01234 GATB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9X100-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRYRPVIGLE IHVQLSTKTK AFCSCPADVF ELPPNTAICP VCTGQPGALP
60 70 80 90 100
VPNEEMIRFA VKTALALNCK IHKYSRFDRK NYFYPDLPKG YQISQYFYPI
110 120 130 140 150
ATEGFLEIDG DEGRKKVRIR RLHLEEDAGK LVHEGDSITR ASYSLVDMNR
160 170 180 190 200
CGVPLIEIVT EPDISSPREA RVFMEKLRSI VRYLGVSTGD MEKGALRCDA
210 220 230 240 250
NISVVDTETG RQSNRVEVKN MNSFRFVERA LEYEFERIVK AMERGEDVER
260 270 280 290 300
ETRGWDMATK ITVSMRGKEE ESDYRYFPEP DIPPVVLSDE YLEEVKKELP
310 320 330 340 350
ELPDEKAERF MREYGLPEYD AKVLTSSKEL AEFFEECVKV VNRPKDLSNW
360 370 380 390 400
IMTEVLRELN ERNIEITESK LTPQHFADLF KLMDEGKISI KIAKEIFPEV
410 420 430 440 450
FETGKMPSQI VEEKGLTQIN DEKLIEELVK KAMEQNPKAV QDYKSGKKKA
460 470 480
AGFFVGYVMR ETKGKANPEL TNRIIQKLLE GE
Length:482
Mass (Da):55,436
Last modified:November 1, 1999 - v1
Checksum:i34988170E32F312F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD36348.1
PIRiH72274
RefSeqiNP_229078.1, NC_000853.1
WP_004079967.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD36348; AAD36348; TM_1273
GeneIDi898210
KEGGitma:TM1273

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD36348.1
PIRiH72274
RefSeqiNP_229078.1, NC_000853.1
WP_004079967.1, NZ_CP011107.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AL0X-ray3.37B1-482[»]
ProteinModelPortaliQ9X100
SMRiQ9X100
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59229N
IntActiQ9X100, 1 interactor
STRINGi243274.TM1273

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36348; AAD36348; TM_1273
GeneIDi898210
KEGGitma:TM1273

Phylogenomic databases

eggNOGiENOG4105CHT Bacteria
COG0064 LUCA
InParanoidiQ9X100
KOiK02434
OMAiSDGNMQE

Miscellaneous databases

EvolutionaryTraceiQ9X100

Family and domain databases

Gene3Di1.10.10.410, 1 hit
HAMAPiMF_00121 GatB, 1 hit
InterProiView protein in InterPro
IPR004413 Apn/Gln-ADT_bsu
IPR017959 Asn/Gln-tRNA_amidoTrfase_suB/E
IPR006075 Asn/Gln-tRNA_Trfase_suB/E_cat
IPR018027 Asn/Gln_amidotransferase
IPR003789 Asn/Gln_tRNA_amidoTrase-B-like
IPR023168 GatB_Yqey_C
IPR017958 Gln-tRNA_amidoTrfase_suB_CS
IPR014746 Gln_synth/guanido_kin_cat_dom
PANTHERiPTHR11659 PTHR11659, 1 hit
PfamiView protein in Pfam
PF02934 GatB_N, 1 hit
PF02637 GatB_Yqey, 1 hit
SMARTiView protein in SMART
SM00845 GatB_Yqey, 1 hit
SUPFAMiSSF55931 SSF55931, 1 hit
SSF89095 SSF89095, 1 hit
TIGRFAMsiTIGR00133 gatB, 1 hit
PROSITEiView protein in PROSITE
PS01234 GATB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGATB_THEMA
AccessioniPrimary (citable) accession number: Q9X100
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: October 10, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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