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Protein

Glutamine-dependent NAD(+) synthetase

Gene

nadE2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (nadE2), Glutamine-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Proton acceptor; for glutaminase activityUniRule annotation1
Active sitei112For glutaminase activityUniRule annotation1
Binding sitei118L-glutamineUniRule annotation1
Active sitei148Nucleophile; for glutaminase activityUniRule annotation1
Binding sitei176L-glutamineUniRule annotation1
Binding sitei182L-glutamineUniRule annotation1
Binding sitei404Deamido-NADUniRule annotation1
Binding sitei428ATPUniRule annotation1
Binding sitei433Deamido-NADUniRule annotation1
Binding sitei545Deamido-NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi321 – 328ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00334

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.5.1UniRule annotation)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]UniRule annotation
Gene namesi
Name:nadE2
Ordered Locus Names:TM_1253
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001522451 – 576Glutamine-dependent NAD(+) synthetaseAdd BLAST576

Proteomic databases

PRIDEiQ9X0Y0

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1253

Structurei

3D structure databases

ProteinModelPortaliQ9X0Y0
SMRiQ9X0Y0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 246CN hydrolasePROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni292 – 576LigaseAdd BLAST285

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C4K Bacteria
COG0171 LUCA
COG0388 LUCA
InParanoidiQ9X0Y0
KOiK01950
OMAiRLAQDCY

Family and domain databases

CDDicd00553 NAD_synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.110.10, 1 hit
HAMAPiMF_02090 NadE_glutamine_dep, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR014445 Gln-dep_NAD_synthase
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR000132 Nitrilase/CN_hydratase_CS
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR23090 PTHR23090, 1 hit
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
PF02540 NAD_synthase, 1 hit
PIRSFiPIRSF006630 NADS_GAT, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
TIGRFAMsiTIGR00552 nadE, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
PS00920 NITRIL_CHT_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9X0Y0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLRVTLAQ LNPTLGDFEG NLKKAIEALR VAEDRGSDLL VFPELFLPGY
60 70 80 90 100
PPEDLMLRLS FLRENRKYLQ KFAQHTRNLG VTVLMGFIDS DEDAYNAAAV
110 120 130 140 150
VKDGEILGVY RKISLPNYGV FDERRYFKPG EELLVVKIGN IKVGVTICED
160 170 180 190 200
IWNPVEPSAS LSLGEGVHLI ANLSASPYHV GKPVLRKDYL SMKAYDYHVA
210 220 230 240 250
MAYCNMVGGQ DELVFDGGSM VVDASGEVIN YGKLFEEEII TVDLDLDENL
260 270 280 290 300
RVSLVDPRRR YMKTQNYPVK TVEAGNLREK SGHFEPVVNP LPVREEEMFR
310 320 330 340 350
ALITGLRDYV RKNGFEKVVI GLSGGMDSSL VAVIATEALG KENVKGVLMP
360 370 380 390 400
SMYTSKESIE DAQTLAKNLG IETFIIPITD VFHSYLETLK GVFAGREPDI
410 420 430 440 450
TEENLQARIR GNYLMALSNK FGWLVLTTGN KSEMATGYAT LYGDMAGGFA
460 470 480 490 500
VIKDVYKTDV YRIGRWYNSW RGKEIIPENI FVKPPTAELR PGQTDQEKLP
510 520 530 540 550
PYEVLDEILR LYIEEGLDPE EIASKGFDRK TVLDVTEMIR KNEYKRKQAA
560 570
IGVKISTRAF GKDWRMPITN RFKEPL
Length:576
Mass (Da):64,920
Last modified:November 1, 1999 - v1
Checksum:i208FDC77964C957F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD36328.1
PIRiG72277
RefSeqiNP_229058.1, NC_000853.1
WP_004080010.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD36328; AAD36328; TM_1253
GeneIDi898230
KEGGitma:TM1253

Similar proteinsi

Entry informationi

Entry nameiNADE2_THEMA
AccessioniPrimary (citable) accession number: Q9X0Y0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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