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UniProtKB - Q9X0C6 (HIS6_THEMA)
Protein
Imidazole glycerol phosphate synthase subunit HisF
Gene
hisF
Organism
Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
Status
Functioni
IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.
Catalytic activityi
- 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H+ + L-glutamateEC:4.3.2.10
Kineticsi
- KM=1.5 µM for PRFAR1 Publication
- KM=2.2 mM for NH31 Publication
: L-histidine biosynthesis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate. This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 11 | Sequence analysis | 1 | |
Active sitei | 130 | Sequence analysis | 1 |
GO - Molecular functioni
- imidazoleglycerol-phosphate synthase activity Source: GO_Central
- lyase activity Source: UniProtKB-KW
GO - Biological processi
- histidine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Enzyme and pathway databases
BRENDAi | 4.3.1.B2, 6331 4.3.2.10, 6331 |
SABIO-RKi | Q9X0C6 |
UniPathwayi | UPA00031;UER00010 |
Names & Taxonomyi
Protein namesi | Recommended name: Imidazole glycerol phosphate synthase subunit HisF (EC:4.3.2.10)Alternative name(s): IGP synthase cyclase subunit IGP synthase subunit HisF ImGP synthase subunit HisF Short name: IGPS subunit HisF |
Gene namesi | Name:hisF Ordered Locus Names:TM_1036 |
Organismi | Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) |
Taxonomic identifieri | 243274 [NCBI] |
Taxonomic lineagei | Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | C → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 11 | D → X: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 19 | K → S: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 51 | D → N: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 103 | N → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 130 | D → A, C, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W or Y: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 130 | D → E: Weak activity. 1 Publication | 1 | |
Mutagenesisi | 176 | D → N: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 183 | D → N: No change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142253 | 1 – 253 | Imidazole glycerol phosphate synthase subunit HisFAdd BLAST | 253 |
Interactioni
Subunit structurei
Heterodimer of HisH and HisF.
Binary interactionsi
Q9X0C6
With | #Exp. | IntAct |
---|---|---|
hisH [Q9X0C8] | 2 | EBI-9026911,EBI-9026913 |
Protein-protein interaction databases
DIPi | DIP-59020N |
IntActi | Q9X0C6, 1 interactor |
STRINGi | 243274.THEMA_09180 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q9X0C6 |
BMRBi | Q9X0C6 |
SMRi | Q9X0C6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9X0C6 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HisA/HisF family.Curated
Phylogenomic databases
eggNOGi | COG0107, Bacteria |
InParanoidi | Q9X0C6 |
OMAi | IFHYKET |
OrthoDBi | 1522718at2 |
Family and domain databases
CDDi | cd04731, HisF, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01013, HisF, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR004651, HisF IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00735, hisF, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9X0C6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAKRIIACL DVKDGRVVKG TNFENLRDSG DPVELGKFYS EIGIDELVFL
60 70 80 90 100
DITASVEKRK TMLELVEKVA EQIDIPFTVG GGIHDFETAS ELILRGADKV
110 120 130 140 150
SINTAAVENP SLITQIAQTF GSQAVVVAID AKRVDGEFMV FTYSGKKNTG
160 170 180 190 200
ILLRDWVVEV EKRGAGEILL TSIDRDGTKS GYDTEMIRFV RPLTTLPIIA
210 220 230 240 250
SGGAGKMEHF LEAFLAGADA ALAASVFHFR EIDVRELKEY LKKHGVNVRL
EGL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD36113.1 |
PIRi | C72304 |
RefSeqi | NP_228842.1, NC_000853.1 WP_004080486.1, NZ_CP011107.1 |
Genome annotation databases
EnsemblBacteriai | AAD36113; AAD36113; TM_1036 |
KEGGi | tma:TM1036 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD36113.1 |
PIRi | C72304 |
RefSeqi | NP_228842.1, NC_000853.1 WP_004080486.1, NZ_CP011107.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GPW | X-ray | 2.40 | A/C/E | 1-253 | [»] | |
1THF | X-ray | 1.45 | D | 1-253 | [»] | |
1VH7 | X-ray | 1.90 | A | 2-253 | [»] | |
2A0N | X-ray | 1.64 | A | 1-253 | [»] | |
2LLE | NMR | - | A | 103-247 | [»] | |
2W6R | X-ray | 2.10 | A | 123-253 | [»] | |
2WJZ | X-ray | 2.60 | A/C/E | 1-253 | [»] | |
3CWO | X-ray | 3.10 | X | 103-253 | [»] | |
3OG3 | X-ray | 2.08 | A | 43-221 | [»] | |
3ZR4 | X-ray | 2.41 | A/C/E | 1-253 | [»] | |
4EWN | X-ray | 1.90 | D | 1-253 | [»] | |
4FX7 | X-ray | 2.08 | A/B/C/D | 99-219 | [»] | |
4J9J | X-ray | 2.30 | A | 91-220 | [»] | |
5TQL | X-ray | 1.90 | A/B | 2-253 | [»] | |
6RTZ | X-ray | 2.87 | A | 1-253 | [»] | |
6RU0 | X-ray | 2.65 | A/C/E | 1-253 | [»] | |
6VDG | X-ray | 2.79 | A | 1-253 | [»] | |
6YMU | X-ray | 2.11 | A/C/E | 1-253 | [»] | |
7AC8 | X-ray | 2.06 | A/C/E | 1-253 | [»] | |
AlphaFoldDBi | Q9X0C6 | |||||
BMRBi | Q9X0C6 | |||||
SMRi | Q9X0C6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-59020N |
IntActi | Q9X0C6, 1 interactor |
STRINGi | 243274.THEMA_09180 |
Genome annotation databases
EnsemblBacteriai | AAD36113; AAD36113; TM_1036 |
KEGGi | tma:TM1036 |
Phylogenomic databases
eggNOGi | COG0107, Bacteria |
InParanoidi | Q9X0C6 |
OMAi | IFHYKET |
OrthoDBi | 1522718at2 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00010 |
BRENDAi | 4.3.1.B2, 6331 4.3.2.10, 6331 |
SABIO-RKi | Q9X0C6 |
Miscellaneous databases
EvolutionaryTracei | Q9X0C6 |
PROi | PR:Q9X0C6 |
Family and domain databases
CDDi | cd04731, HisF, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01013, HisF, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR004651, HisF IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00735, hisF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS6_THEMA | |
Accessioni | Q9X0C6Primary (citable) accession number: Q9X0C6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | November 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families