UniProtKB - Q9WZW0 (TRUB_THEMA)
Protein
tRNA pseudouridine synthase B
Gene
truB
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Functioni
Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation
Catalytic activityi
- EC:5.4.99.25UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 39 | NucleophileUniRule annotation | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: GO_Central
- RNA binding Source: InterPro
- tRNA pseudouridine synthase activity Source: UniProtKB-EC
GO - Biological processi
- mRNA pseudouridine synthesis Source: GO_Central
- tRNA modification Source: GO_Central
- tRNA pseudouridine synthesis Source: UniProtKB-UniRule
Keywordsi
Molecular function | Isomerase |
Biological process | tRNA processing |
Enzyme and pathway databases
BRENDAi | 5.4.99.25, 6331 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)Alternative name(s): tRNA pseudouridine(55) synthase1 PublicationUniRule annotation Short name: Psi55 synthaseUniRule annotation tRNA pseudouridylate synthaseUniRule annotation tRNA-uridine isomeraseUniRule annotation |
Gene namesi | Name:truB1 PublicationUniRule annotation Ordered Locus Names:TM_0856 |
Organismi | Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) |
Taxonomic identifieri | 243274 [NCBI] |
Taxonomic lineagei | Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121929 | 1 – 309 | tRNA pseudouridine synthase BAdd BLAST | 309 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9WZW0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9WZW0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 229 – 306 | PUAUniRule annotationAdd BLAST | 78 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0130, Bacteria |
InParanoidi | Q9WZW0 |
OMAi | IMQVPSK |
OrthoDBi | 1166299at2 |
Family and domain databases
CDDi | cd02573, PseudoU_synth_EcTruB, 1 hit |
Gene3Di | 2.30.130.10, 1 hit |
HAMAPi | MF_01080, TruB_bact, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR002501, PsdUridine_synth_N IPR002478, PUA IPR015947, PUA-like_sf IPR036974, PUA_sf IPR014780, tRNA_psdUridine_synth_TruB IPR032819, TruB_C |
PANTHERi | PTHR13767, PTHR13767, 1 hit |
Pfami | View protein in Pfam PF01472, PUA, 1 hit PF16198, TruB_C_2, 1 hit PF01509, TruB_N, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00431, TruB, 1 hit |
PROSITEi | View protein in PROSITE PS50890, PUA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9WZW0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKHGILVAYK PKGPTSHDVV DEVRKKLKTR KVGHGGTLDP FACGVLIIGV
60 70 80 90 100
NQGTRILEFY KDLKKVYWVK MRLGLITETF DITGEVVEER ECNVTEEEIR
110 120 130 140 150
EAIFSFVGEY DQVPPAYSAK KYKGERLYKL AREGKIINLP PKRVKIFKIW
160 170 180 190 200
DVNIEGRDVS FRVEVSPGTY IRSLCMDIGY KLGCGATAVE LVRESVGPHT
210 220 230 240 250
IEESLNVFEA APEEIENRII PLEKCLEWLP RVVVHQESTK MILNGSQIHL
260 270 280 290 300
EMLKEWDGFK KGEVVRVFNE EGRLLALAEA ERNSSFLETL RKHERNERVL
TLRKVFNTR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD35938.1 |
PIRi | A72325 |
RefSeqi | NP_228665.1, NC_000853.1 WP_004080766.1, NZ_CP011107.1 |
Genome annotation databases
EnsemblBacteriai | AAD35938; AAD35938; TM_0856 |
KEGGi | tma:TM0856 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD35938.1 |
PIRi | A72325 |
RefSeqi | NP_228665.1, NC_000853.1 WP_004080766.1, NZ_CP011107.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R3E | X-ray | 2.10 | A | 1-309 | [»] | |
1ZE1 | X-ray | 2.90 | A/B/C/D | 1-309 | [»] | |
1ZE2 | X-ray | 3.00 | A/B | 1-309 | [»] | |
2AB4 | X-ray | 2.40 | A | 1-309 | [»] | |
SMRi | Q9WZW0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 243274.THEMA_00355 |
Genome annotation databases
EnsemblBacteriai | AAD35938; AAD35938; TM_0856 |
KEGGi | tma:TM0856 |
Phylogenomic databases
eggNOGi | COG0130, Bacteria |
InParanoidi | Q9WZW0 |
OMAi | IMQVPSK |
OrthoDBi | 1166299at2 |
Enzyme and pathway databases
BRENDAi | 5.4.99.25, 6331 |
Miscellaneous databases
EvolutionaryTracei | Q9WZW0 |
Family and domain databases
CDDi | cd02573, PseudoU_synth_EcTruB, 1 hit |
Gene3Di | 2.30.130.10, 1 hit |
HAMAPi | MF_01080, TruB_bact, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR002501, PsdUridine_synth_N IPR002478, PUA IPR015947, PUA-like_sf IPR036974, PUA_sf IPR014780, tRNA_psdUridine_synth_TruB IPR032819, TruB_C |
PANTHERi | PTHR13767, PTHR13767, 1 hit |
Pfami | View protein in Pfam PF01472, PUA, 1 hit PF16198, TruB_C_2, 1 hit PF01509, TruB_N, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00431, TruB, 1 hit |
PROSITEi | View protein in PROSITE PS50890, PUA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRUB_THEMA | |
Accessioni | Q9WZW0Primary (citable) accession number: Q9WZW0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 4, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families