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UniProtKB - Q9WYV8 (PRMC_THEMA)
Protein
Release factor glutamine methyltransferase
Gene
prmC
Organism
Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
Status
Functioni
Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.
CuratedMiscellaneous
The crystal structure corresponding to PDB 1SG9 shows a glutamate anhydride cross-link formed between the Glu-97 side chains from two molecules, but this has not been observed in other PrmC structures from T.maritima. This cross-link is suggested being an artifact of concentration during crystallization (PubMed:18247349).1 Publication
Catalytic activityi
- L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H+ + N5-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteineEC:2.1.1.297
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 151 | S-adenosyl-L-methionine2 Publications | 1 | |
Binding sitei | 180 | S-adenosyl-L-methionine2 Publications | 1 | |
Binding sitei | 197 | S-adenosyl-L-methionine2 Publications | 1 |
GO - Molecular functioni
- nucleic acid binding Source: InterPro
- protein-(glutamine-N5) methyltransferase activity Source: UniProtKB-EC
- protein-glutamine N-methyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- peptidyl-glutamine methylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.297, 6331 |
Names & Taxonomyi
Protein namesi | Recommended name: Release factor glutamine methyltransferase (EC:2.1.1.297)Short name: RF MTase Alternative name(s): N5-glutamine methyltransferase PrmC Protein-(glutamine-N5) MTase PrmC Protein-glutamine N-methyltransferase PrmC |
Gene namesi | Name:prmC Ordered Locus Names:TM_0488 |
Organismi | Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) |
Taxonomic identifieri | 243274 [NCBI] |
Taxonomic lineagei | Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
DrugBanki | DB03473, N5-Methylglutamine DB01752, S-adenosyl-L-homocysteine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000414548 | 1 – 282 | Release factor glutamine methyltransferaseAdd BLAST | 282 |
Interactioni
Subunit structurei
Monomer and homodimer.
2 PublicationsProtein-protein interaction databases
STRINGi | 243274.THEMA_02230 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9WYV8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9WYV8 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 129 – 133 | S-adenosyl-L-methionine binding | 5 | |
Regioni | 197 – 200 | Substrate binding | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2890, Bacteria |
InParanoidi | Q9WYV8 |
OMAi | IYYGHGT |
OrthoDBi | 1816476at2 |
Family and domain databases
HAMAPi | MF_02126, RF_methyltr_PrmC, 1 hit |
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR004556, HemK-like IPR040758, PrmC_N IPR019874, Release_fac_Glu-N5_MeTfrase IPR029063, SAM-dependent_MTases IPR007848, Small_mtfrase_dom |
Pfami | View protein in Pfam PF05175, MTS, 1 hit PF17827, PrmC_N, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00536, hemK_fam, 1 hit TIGR03534, RF_mod_PrmC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9WYV8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDTRKNVSGA ERKIWSLIRD CSGKLEGVTE TSVLEVLLIV SRVLGIRKED
60 70 80 90 100
LFLKDLGVSP TEEKRILELV EKRASGYPLH YILGEKEFMG LSFLVEEGVF
110 120 130 140 150
VPRPETEELV ELALELIRKY GIKTVADIGT GSGAIGVSVA KFSDAIVFAT
160 170 180 190 200
DVSSKAVEIA RKNAERHGVS DRFFVRKGEF LEPFKEKFAS IEMILSNPPY
210 220 230 240 250
VKSSAHLPKD VLFEPPEALF GGEDGLDFYR EFFGRYDTSG KIVLMEIGED
260 270 280
QVEELKKIVS DTVFLKDSAG KYRFLLLNRR SS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD35573.1 |
PIRi | G72369 |
RefSeqi | NP_228298.1, NC_000853.1 WP_004081478.1, NZ_CP011107.1 |
Genome annotation databases
EnsemblBacteriai | AAD35573; AAD35573; TM_0488 |
KEGGi | tma:TM0488 tmw:THMA_0501 |
PATRICi | fig|243274.18.peg.442 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD35573.1 |
PIRi | G72369 |
RefSeqi | NP_228298.1, NC_000853.1 WP_004081478.1, NZ_CP011107.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NV8 | X-ray | 2.20 | A/B | 1-282 | [»] | |
1NV9 | X-ray | 2.36 | A | 1-282 | [»] | |
1SG9 | X-ray | 2.30 | A/B/C | 1-282 | [»] | |
1VQ1 | X-ray | 2.80 | A/B | 1-282 | [»] | |
SMRi | Q9WYV8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 243274.THEMA_02230 |
Chemistry databases
DrugBanki | DB03473, N5-Methylglutamine DB01752, S-adenosyl-L-homocysteine |
Protocols and materials databases
DNASUi | 897528 |
Genome annotation databases
EnsemblBacteriai | AAD35573; AAD35573; TM_0488 |
KEGGi | tma:TM0488 tmw:THMA_0501 |
PATRICi | fig|243274.18.peg.442 |
Phylogenomic databases
eggNOGi | COG2890, Bacteria |
InParanoidi | Q9WYV8 |
OMAi | IYYGHGT |
OrthoDBi | 1816476at2 |
Enzyme and pathway databases
BRENDAi | 2.1.1.297, 6331 |
Miscellaneous databases
EvolutionaryTracei | Q9WYV8 |
Family and domain databases
HAMAPi | MF_02126, RF_methyltr_PrmC, 1 hit |
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR004556, HemK-like IPR040758, PrmC_N IPR019874, Release_fac_Glu-N5_MeTfrase IPR029063, SAM-dependent_MTases IPR007848, Small_mtfrase_dom |
Pfami | View protein in Pfam PF05175, MTS, 1 hit PF17827, PrmC_N, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00536, hemK_fam, 1 hit TIGR03534, RF_mod_PrmC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRMC_THEMA | |
Accessioni | Q9WYV8Primary (citable) accession number: Q9WYV8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 14, 2011 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families