UniProtKB - Q9WYH8 (AROF_THEMA)
Protein
Phospho-2-dehydro-3-deoxyheptonate aldolase
Gene
aroF
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Functioni
Catalyzes the condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Catalytic activityi
- D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphateEC:2.5.1.54
Cofactori
a divalent metal cationNote: Divalent metal ions.
Activity regulationi
Inhibited by L-phenylalanine and L-tyrosine.
pH dependencei
Optimum pH is 6.3 at 60 degrees Celsius.
Temperature dependencei
Optimum temperature is 90 degrees Celsius. Extremely thermostable.
: chorismate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.Proteins known to be involved in the 7 steps of the subpathway in this organism are:
- Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF)
- Bifunctional shikimate kinase/3-dehydroquinate synthase (aroKB)
- 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
- Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
- Shikimate kinase (aroK), Bifunctional shikimate kinase/3-dehydroquinate synthase (aroKB)
- 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
- Chorismate synthase (aroC), Chorismate synthase (aroC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.
GO - Molecular functioni
- 3-deoxy-7-phosphoheptulonate synthase activity Source: UniProtKB-EC
- aldehyde-lyase activity Source: InterPro
GO - Biological processi
- aromatic amino acid family biosynthetic process Source: UniProtKB-KW
- chorismate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00053;UER00084 |
Names & Taxonomyi
Protein namesi | Recommended name: Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54)Alternative name(s): 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DAHP synthase Phospho-2-keto-3-deoxyheptonate aldolase |
Gene namesi | Name:aroF Ordered Locus Names:TM_0343 |
Organismi | Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) |
Taxonomic identifieri | 243274 [NCBI] |
Taxonomic lineagei | Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
DrugBanki | DB03937, D-erythrose 4-phosphate DB01819, Phosphoenolpyruvate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000140847 | 1 – 338 | Phospho-2-dehydro-3-deoxyheptonate aldolaseAdd BLAST | 338 |
Interactioni
Subunit structurei
Homotetramer.
Protein-protein interaction databases
STRINGi | 243274.THEMA_03000 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9WYH8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9WYH8 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I DAHP synthase family.Curated
Phylogenomic databases
eggNOGi | COG2876, Bacteria |
InParanoidi | Q9WYH8 |
OMAi | HISQGVE |
OrthoDBi | 687380at2 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006218, DAHP1/KDSA IPR041071, DAHP_snth_FXD IPR006268, DAHP_syn_2 |
Pfami | View protein in Pfam PF18152, DAHP_snth_FXD, 1 hit PF00793, DAHP_synth_1, 1 hit |
TIGRFAMsi | TIGR01361, DAHP_synth_Bsub, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9WYH8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVVLKPGST EEDIRKVVKL AESYNLKCHI SKGQERTVIG IIGDDRYVVA
60 70 80 90 100
DKFESLDCVE SVVRVLKPYK LVSREFHPED TVIDLGDVKI GNGYFTIIAG
110 120 130 140 150
PCSVEGREML METAHFLSEL GVKVLRGGAY KPRTSPYSFQ GLGEKGLEYL
160 170 180 190 200
REAADKYGMY VVTEALGEDD LPKVAEYADI IQIGARNAQN FRLLSKAGSY
210 220 230 240 250
NKPVLLKRGF MNTIEEFLLS AEYIANSGNT KIILCERGIR TFEKATRNTL
260 270 280 290 300
DISAVPIIRK ESHLPILVDP SHSGGRRDLV IPLSRAAIAV GAHGIIVEVH
310 320 330
PEPEKALSDG KQSLDFELFK ELVQEMKKLA DALGVKVN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD35429.1 |
PIRi | E72388 |
RefSeqi | NP_228154.1, NC_000853.1 WP_004083125.1, NZ_CP011107.1 |
Genome annotation databases
EnsemblBacteriai | AAD35429; AAD35429; TM_0343 |
KEGGi | tma:TM0343 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD35429.1 |
PIRi | E72388 |
RefSeqi | NP_228154.1, NC_000853.1 WP_004083125.1, NZ_CP011107.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RZM | X-ray | 2.20 | A/B | 1-338 | [»] | |
1VR6 | X-ray | 1.92 | A/B/C/D | 1-338 | [»] | |
3PG8 | X-ray | 2.00 | A/B | 71-338 | [»] | |
3PG9 | X-ray | 2.35 | A/B/C/D/E/F/G/H | 1-338 | [»] | |
4GRS | X-ray | 3.00 | A/B/C/D | 1-103 | [»] | |
SMRi | Q9WYH8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 243274.THEMA_03000 |
Chemistry databases
DrugBanki | DB03937, D-erythrose 4-phosphate DB01819, Phosphoenolpyruvate |
Genome annotation databases
EnsemblBacteriai | AAD35429; AAD35429; TM_0343 |
KEGGi | tma:TM0343 |
Phylogenomic databases
eggNOGi | COG2876, Bacteria |
InParanoidi | Q9WYH8 |
OMAi | HISQGVE |
OrthoDBi | 687380at2 |
Enzyme and pathway databases
UniPathwayi | UPA00053;UER00084 |
Miscellaneous databases
EvolutionaryTracei | Q9WYH8 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006218, DAHP1/KDSA IPR041071, DAHP_snth_FXD IPR006268, DAHP_syn_2 |
Pfami | View protein in Pfam PF18152, DAHP_snth_FXD, 1 hit PF00793, DAHP_synth_1, 1 hit |
TIGRFAMsi | TIGR01361, DAHP_synth_Bsub, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AROF_THEMA | |
Accessioni | Q9WYH8Primary (citable) accession number: Q9WYH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 24, 2003 |
Last sequence update: | November 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families