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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase

Gene

aroF

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Cofactori

a divalent metal cationNote: Divalent metal ions.

Activity regulationi

Inhibited by L-phenylalanine and L-tyrosine.

pH dependencei

Optimum pH is 6.3 at 60 degrees Celsius.

Temperature dependencei

Optimum temperature is 90 degrees Celsius. Extremely thermostable.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF)
  2. Bifunctional shikimate kinase/3-dehydroquinate synthase (aroKB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Bifunctional shikimate kinase/3-dehydroquinate synthase (aroKB), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00084

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:aroF
Ordered Locus Names:TM_0343
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB03937 Erythose-4-Phosphate
DB01819 Phosphoenolpyruvate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001408471 – 338Phospho-2-dehydro-3-deoxyheptonate aldolaseAdd BLAST338

Proteomic databases

PRIDEiQ9WYH8

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi243274.TM0343

Structurei

Secondary structure

1338
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9WYH8
SMRiQ9WYH8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYH8

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4108JPM Bacteria
COG2876 LUCA
InParanoidiQ9WYH8
KOiK03856
OMAiVIVMKPN

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006268 DAHP_syn_2
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
TIGRFAMsiTIGR01361 DAHP_synth_Bsub, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WYH8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIVVLKPGST EEDIRKVVKL AESYNLKCHI SKGQERTVIG IIGDDRYVVA
60 70 80 90 100
DKFESLDCVE SVVRVLKPYK LVSREFHPED TVIDLGDVKI GNGYFTIIAG
110 120 130 140 150
PCSVEGREML METAHFLSEL GVKVLRGGAY KPRTSPYSFQ GLGEKGLEYL
160 170 180 190 200
REAADKYGMY VVTEALGEDD LPKVAEYADI IQIGARNAQN FRLLSKAGSY
210 220 230 240 250
NKPVLLKRGF MNTIEEFLLS AEYIANSGNT KIILCERGIR TFEKATRNTL
260 270 280 290 300
DISAVPIIRK ESHLPILVDP SHSGGRRDLV IPLSRAAIAV GAHGIIVEVH
310 320 330
PEPEKALSDG KQSLDFELFK ELVQEMKKLA DALGVKVN
Length:338
Mass (Da):37,378
Last modified:November 1, 1999 - v1
Checksum:iE9634B6704D3DF4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD35429.1
PIRiE72388
RefSeqiNP_228154.1, NC_000853.1
WP_004083125.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD35429; AAD35429; TM_0343
GeneIDi29653123
897299
KEGGitma:TM0343

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD35429.1
PIRiE72388
RefSeqiNP_228154.1, NC_000853.1
WP_004083125.1, NZ_CP011107.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RZMX-ray2.20A/B1-338[»]
1VR6X-ray1.92A/B/C/D1-338[»]
3PG8X-ray2.00A/B71-338[»]
3PG9X-ray2.35A/B/C/D/E/F/G/H1-338[»]
4GRSX-ray3.00A/B/C/D1-103[»]
ProteinModelPortaliQ9WYH8
SMRiQ9WYH8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0343

Chemistry databases

DrugBankiDB03937 Erythose-4-Phosphate
DB01819 Phosphoenolpyruvate

Proteomic databases

PRIDEiQ9WYH8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35429; AAD35429; TM_0343
GeneIDi29653123
897299
KEGGitma:TM0343

Phylogenomic databases

eggNOGiENOG4108JPM Bacteria
COG2876 LUCA
InParanoidiQ9WYH8
KOiK03856
OMAiVIVMKPN

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00084

Miscellaneous databases

EvolutionaryTraceiQ9WYH8

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006268 DAHP_syn_2
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
TIGRFAMsiTIGR01361 DAHP_synth_Bsub, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAROF_THEMA
AccessioniPrimary (citable) accession number: Q9WYH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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