UniProtKB - Q9WYH8 (AROF_THEMA)

BLAST

>sp|Q9WYH8|AROF_THEMA Phospho-2-dehydro-3-deoxyheptonate aldolase OS=Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) OX=243274 GN=aroF PE=1 SV=1
MIVVLKPGSTEEDIRKVVKLAESYNLKCHISKGQERTVIGIIGDDRYVVADKFESLDCVE
SVVRVLKPYKLVSREFHPEDTVIDLGDVKIGNGYFTIIAGPCSVEGREMLMETAHFLSEL
GVKVLRGGAYKPRTSPYSFQGLGEKGLEYLREAADKYGMYVVTEALGEDDLPKVAEYADI
IQIGARNAQNFRLLSKAGSYNKPVLLKRGFMNTIEEFLLSAEYIANSGNTKIILCERGIR
TFEKATRNTLDISAVPIIRKESHLPILVDPSHSGGRRDLVIPLSRAAIAVGAHGIIVEVH
PEPEKALSDGKQSLDFELFKELVQEMKKLADALGVKVN

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History
Entry version 121 (07 Nov 2018)
Sequence version 1 (01 Nov 1999)
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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase

Gene

aroF

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activityⁱ

Phosphoenolpyruvate + D-erythrose 4-phosphate + H₂O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Cofactorⁱ

a divalent metal cationNote: Divalent metal ions.

Activity regulationⁱ

Inhibited by L-phenylalanine and L-tyrosine.

pH dependenceⁱ

Optimum pH is 6.3 at 60 degrees Celsius.

Temperature dependenceⁱ

Optimum temperature is 90 degrees Celsius. Extremely thermostable.

Pathwayⁱ: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:

This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functionⁱ

3-deoxy-7-phosphoheptulonate synthase activity Source: UniProtKB-EC
aldehyde-lyase activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aromatic amino acid family biosynthetic process Source: UniProtKB-KW
chorismate biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayⁱ	UPA00053;UER00084

UniPathwayⁱ

UPA00053;UER00084

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54) Alternative name(s): 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DAHP synthase Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesⁱ	Name:aroF Ordered Locus Names:TM_0343
Organismⁱ	Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifierⁱ	243274 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima
Proteomesⁱ	UP000008183 Componentⁱ: Chromosome

Pathology & Biotechⁱ

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB03937 Erythose-4-Phosphate DB01819 Phosphoenolpyruvate

DrugBankⁱ

DB03937 Erythose-4-Phosphate
DB01819 Phosphoenolpyruvate

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000140847	1 – 338	Phospho-2-dehydro-3-deoxyheptonate aldolaseAdd BLAST		338

Proteomic databases

PRIDEⁱ	Q9WYH8

PRIDEⁱ

Q9WYH8

Interactionⁱ

Subunit structureⁱ

Homotetramer.

Protein-protein interaction databases

STRINGⁱ	243274.TM0343

STRINGⁱ

243274.TM0343

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	1 – 5	Combined sources	5
Helixⁱ	11 – 23	Combined sources	13
Beta strandⁱ	26 – 32	Combined sources	7
Beta strandⁱ	37 – 47	Combined sources	11
Helixⁱ	50 – 54	Combined sources	5
Beta strandⁱ	59 – 64	Combined sources	6
Turnⁱ	74 – 76	Combined sources	3
Beta strandⁱ	82 – 84	Combined sources	3
Beta strandⁱ	89 – 91	Combined sources	3
Beta strandⁱ	94 – 100	Combined sources	7
Helixⁱ	107 – 119	Combined sources	13
Beta strandⁱ	124 – 126	Combined sources	3
Beta strandⁱ	128 – 130	Combined sources	3
Helixⁱ	144 – 157	Combined sources	14
Beta strandⁱ	160 – 164	Combined sources	5
Helixⁱ	168 – 170	Combined sources	3
Helixⁱ	171 – 177	Combined sources	7
Beta strandⁱ	179 – 183	Combined sources	5
Helixⁱ	185 – 187	Combined sources	3
Helixⁱ	191 – 198	Combined sources	8
Beta strandⁱ	204 – 207	Combined sources	4
Helixⁱ	214 – 226	Combined sources	13
Beta strandⁱ	232 – 236	Combined sources	5
Beta strandⁱ	245 – 249	Combined sources	5
Helixⁱ	254 – 261	Combined sources	8
Beta strandⁱ	262 – 264	Combined sources	3
Beta strandⁱ	266 – 268	Combined sources	3
Helixⁱ	270 – 274	Combined sources	5
Helixⁱ	277 – 279	Combined sources	3
Helixⁱ	280 – 290	Combined sources	11
Beta strandⁱ	293 – 299	Combined sources	7
Helixⁱ	303 – 305	Combined sources	3
Helixⁱ	310 – 312	Combined sources	3
Helixⁱ	316 – 333	Combined sources	18
Beta strandⁱ	336 – 338	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9WYH8
SMRⁱ	Q9WYH8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9WYH8

EvolutionaryTraceⁱ

Q9WYH8

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108JPM Bacteria COG2876 LUCA
InParanoidⁱ	Q9WYH8
KEGG Orthology (KO) More...KOⁱ	K03856
OMAⁱ	VIVMKPN

Family and domain databases

Gene3Dⁱ	3.20.20.70, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR006218 DAHP1/KDSA IPR006268 DAHP_syn_2
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00793 DAHP_synth_1, 1 hit
TIGRFAMsⁱ	TIGR01361 DAHP_synth_Bsub, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9WYH8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIVVLKPGST EEDIRKVVKL AESYNLKCHI SKGQERTVIG IIGDDRYVVA 
        60         70         80         90        100
DKFESLDCVE SVVRVLKPYK LVSREFHPED TVIDLGDVKI GNGYFTIIAG 
       110        120        130        140        150
PCSVEGREML METAHFLSEL GVKVLRGGAY KPRTSPYSFQ GLGEKGLEYL 
       160        170        180        190        200
REAADKYGMY VVTEALGEDD LPKVAEYADI IQIGARNAQN FRLLSKAGSY 
       210        220        230        240        250
NKPVLLKRGF MNTIEEFLLS AEYIANSGNT KIILCERGIR TFEKATRNTL 
       260        270        280        290        300
DISAVPIIRK ESHLPILVDP SHSGGRRDLV IPLSRAAIAV GAHGIIVEVH 
       310        320        330 
PEPEKALSDG KQSLDFELFK ELVQEMKKLA DALGVKVN

Length:338

Mass (Da):37,378

Last modified:November 1, 1999 - v1

Checksum:ⁱE9634B6704D3DF4D

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE000512 Genomic DNA Translation: AAD35429.1
PIRⁱ	E72388
NCBI Reference Sequences More...RefSeqⁱ	NP_228154.1, NC_000853.1 WP_004083125.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaⁱ	AAD35429; AAD35429; TM_0343
GeneIDⁱ	29653123 897299
KEGGⁱ	tma:TM0343

Protein	Similar proteins		Species	Score	Length	Source
Q9WYH8	Phospho-2-dehydro-3-deoxyheptonate aldolase		THENN		380	UniRef100_Q9WYH8
Phospho-2-dehydro-3-deoxyheptonate aldolase		Thermotoga sp. 47_83		338
Phospho-2-dehydro-3-deoxyheptonate aldolase		Thermotoga naphthophila		338
3-deoxy-D-arabinoheptulosonate-7-phosphate synthase		THEP1		338
Phospho-2-dehydro-3-deoxyheptonate aldolase		THENR		338
+3

Protein	Similar proteins		Species	Score	Length	Source
Q9WYH8	Phospho-2-dehydro-3-deoxyheptonate aldolase		THENN		380	UniRef90_Q9WYH8
Phospho-2-dehydro-3-deoxyheptonate aldolase		Thermotoga sp. 47_83		338
Phospho-2-dehydro-3-deoxyheptonate aldolase		Thermotoga naphthophila		338
3-deoxy-D-arabinoheptulosonate-7-phosphate synthase		THEP1		338
Phospho-2-dehydro-3-deoxyheptonate aldolase		THENR		338
+7

Protein	Similar proteins		Species	Score	Length	Source
Q9WYH8	Phospho-2-dehydro-3-deoxyheptonate aldolase		THENN		380	UniRef50_Q9WYH8
Phospho-2-dehydro-3-deoxyheptonate aldolase		Thermotoga sp. 47_83		338
Phospho-2-dehydro-3-deoxyheptonate aldolase		Thermotoga naphthophila		338
3-deoxy-D-arabinoheptulosonate-7-phosphate synthase		THEP1		338
Phospho-2-dehydro-3-deoxyheptonate aldolase		THENR		338
+185

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE000512 Genomic DNA Translation: AAD35429.1
PIRⁱ	E72388
RefSeqⁱ	NP_228154.1, NC_000853.1 WP_004083125.1, NZ_CP011107.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1RZM	X-ray	2.20	A/B	1-338	[»]
	1VR6	X-ray	1.92	A/B/C/D	1-338	[»]
	3PG8	X-ray	2.00	A/B	71-338	[»]
	3PG9	X-ray	2.35	A/B/C/D/E/F/G/H	1-338	[»]
	4GRS	X-ray	3.00	A/B/C/D	1-103	[»]
ProteinModelPortalⁱ	Q9WYH8
SMRⁱ	Q9WYH8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	243274.TM0343

STRINGⁱ

243274.TM0343

Chemistry databases

DrugBankⁱ	DB03937 Erythose-4-Phosphate DB01819 Phosphoenolpyruvate

DrugBankⁱ

DB03937 Erythose-4-Phosphate
DB01819 Phosphoenolpyruvate

Proteomic databases

PRIDEⁱ	Q9WYH8

PRIDEⁱ

Q9WYH8

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAD35429; AAD35429; TM_0343
GeneIDⁱ	29653123 897299
KEGGⁱ	tma:TM0343

Phylogenomic databases

eggNOGⁱ	ENOG4108JPM Bacteria COG2876 LUCA
InParanoidⁱ	Q9WYH8
KOⁱ	K03856
OMAⁱ	VIVMKPN

Enzyme and pathway databases

UniPathwayⁱ	UPA00053;UER00084

UniPathwayⁱ

UPA00053;UER00084

Miscellaneous databases

EvolutionaryTraceⁱ	Q9WYH8

EvolutionaryTraceⁱ

Q9WYH8

Family and domain databases

Gene3Dⁱ	3.20.20.70, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR006218 DAHP1/KDSA IPR006268 DAHP_syn_2
Pfamⁱ	View protein in Pfam PF00793 DAHP_synth_1, 1 hit
TIGRFAMsⁱ	TIGR01361 DAHP_synth_Bsub, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AROF_THEMA
Accessionⁱ	Q9WYH8Primary (citable) accession number: Q9WYH8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
	Last sequence update:	November 1, 1999
	Last modified:	November 7, 2018
	This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9WYH8 (AROF_THEMA)

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Phospho-2-dehydro-3-deoxyheptonate aldolase

aroF

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Temperature dependencei

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: chorismate biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

pH dependenceⁱ

Temperature dependenceⁱ

Pathwayⁱ: chorismate biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ