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UniProtKB - Q9WXM1 (AEEP_THEMA)
Protein
L-Ala-D/L-Glu epimerase
Gene
TM_0006
Organism
Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
Status
Functioni
Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L-Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of a broad range of other dipeptides; is most efficient with L-Ala-D/L-Phe, L-Ala-D/L-Tyr, and L-Ala-D/L-His.
1 PublicationCatalytic activityi
- EC:5.1.1.202 Publications
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
Kineticsi
- KM=2.8 mM for L-Ala-L-Glu (at pH 7.5 and 28 degrees Celsius)1 Publication
- KM=1.3 mM for L-Ala-L-Phe (at pH 7.5 and 28 degrees Celsius)1 Publication
- KM=0.71 mM for L-Ala-L-Tyr (at pH 7.5 and 28 degrees Celsius)1 Publication
- KM=5.3 mM for L-Ala-L-His (at pH 7.5 and 28 degrees Celsius)1 Publication
- KM=2.9 mM for L-Ala-L-Leu (at pH 7.5 and 28 degrees Celsius)1 Publication
- KM=4.7 mM for L-Ile-L-Phe (at pH 7.5 and 28 degrees Celsius)1 Publication
- KM=3.9 mM for L-Lys-L-Phe (at pH 7.5 and 28 degrees Celsius)1 Publication
: peptidoglycan degradation Pathwayi
This protein is involved in the pathway peptidoglycan degradation, which is part of Cell wall degradation.View all proteins of this organism that are known to be involved in the pathway peptidoglycan degradation and in Cell wall degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 134 | Substrate | 1 | |
Binding sitei | 159 | Substrate | 1 | |
Active sitei | 161 | Proton acceptor; specific for (R)-substrate epimerizationBy similarity | 1 | |
Metal bindingi | 188 | Magnesium | 1 | |
Binding sitei | 190 | Substrate | 1 | |
Metal bindingi | 216 | Magnesium | 1 | |
Metal bindingi | 241 | Magnesium | 1 | |
Active sitei | 265 | Proton acceptor; specific for (S)-substrate epimerizationBy similarity | 1 | |
Binding sitei | 292 | Substrate; via carbonyl oxygen | 1 | |
Binding sitei | 317 | Substrate | 1 | |
Binding sitei | 319 | Substrate | 1 |
GO - Molecular functioni
- L-Ala-D/L-Glu epimerase activity Source: RHEA
- metal ion binding Source: UniProtKB-KW
- racemase and epimerase activity, acting on amino acids and derivatives Source: CACAO
GO - Biological processi
- cell wall macromolecule catabolic process Source: UniProtKB-UniPathway
- cell wall organization Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Biological process | Cell wall biogenesis/degradation |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
SABIO-RKi | Q9WXM1 |
UniPathwayi | UPA00549 |
Names & Taxonomyi
Protein namesi | Recommended name: L-Ala-D/L-Glu epimerase (EC:5.1.1.202 Publications)Short name: AE epimerase Short name: AEE |
Gene namesi | Ordered Locus Names:TM_0006 |
Organismi | Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) |
Taxonomic identifieri | 243274 [NCBI] |
Taxonomic lineagei | Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000388972 | 1 – 345 | L-Ala-D/L-Glu epimeraseAdd BLAST | 345 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9WXM1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9WXM1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the mandelate racemase/muconate lactonizing enzyme family.Curated
Phylogenomic databases
eggNOGi | COG4948, Bacteria |
InParanoidi | Q9WXM1 |
OMAi | MFGCYSD |
OrthoDBi | 951991at2 |
Family and domain databases
CDDi | cd03319, L-Ala-DL-Glu_epimerase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR034603, Dipeptide_epimerase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR013342, Mandelate_racemase_C IPR013341, Mandelate_racemase_N_dom |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9WXM1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRIVNVKLS LKRYEYEKPF HITGSVSSES RNVEVEIVLE SGVKGYGEAS
60 70 80 90 100
PSFRVNGERV EALLAIENAV REMITGIDVR NYARIFEITD RLFGFPSLKA
110 120 130 140 150
AVQFATLDAL SQELGTQVCY LLGGKRDEIE TDKTVGIDTV ENRVKEAKKI
160 170 180 190 200
FEEGFRVIKI KVGENLKEDI EAVEEIAKVT RGAKYIVDAN MGYTQKEAVE
210 220 230 240 250
FARAVYQKGI DIAVYEQPVR REDIEGLKFV RFHSPFPVAA DESARTKFDV
260 270 280 290 300
MRLVKEEAVD YVNIKLMKSG ISDALAIVEI AESSGLKLMI GCMGESSLGI
310 320 330 340
NQSVHFALGT GAFEFHDLDS HLMLKEEVFR GKFIQDGPRM RVKDQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD35100.1 |
PIRi | H72429 |
RefSeqi | NP_227822.1, NC_000853.1 WP_004082436.1, NZ_CP011107.1 |
Genome annotation databases
EnsemblBacteriai | AAD35100; AAD35100; TM_0006 |
KEGGi | tma:TM0006 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000512 Genomic DNA Translation: AAD35100.1 |
PIRi | H72429 |
RefSeqi | NP_227822.1, NC_000853.1 WP_004082436.1, NZ_CP011107.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZAD | X-ray | 1.60 | A/B/C/D | 1-345 | [»] | |
3DEQ | X-ray | 2.10 | A/B/C/D | 1-345 | [»] | |
3DER | X-ray | 1.90 | A/B/C/D | 1-345 | [»] | |
3DES | X-ray | 2.30 | A/B/C/D | 1-345 | [»] | |
3DFY | X-ray | 2.10 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P | 1-345 | [»] | |
SMRi | Q9WXM1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 243274.THEMA_04770 |
Protocols and materials databases
DNASUi | 896814 |
Genome annotation databases
EnsemblBacteriai | AAD35100; AAD35100; TM_0006 |
KEGGi | tma:TM0006 |
Phylogenomic databases
eggNOGi | COG4948, Bacteria |
InParanoidi | Q9WXM1 |
OMAi | MFGCYSD |
OrthoDBi | 951991at2 |
Enzyme and pathway databases
UniPathwayi | UPA00549 |
SABIO-RKi | Q9WXM1 |
Miscellaneous databases
EvolutionaryTracei | Q9WXM1 |
Family and domain databases
CDDi | cd03319, L-Ala-DL-Glu_epimerase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR034603, Dipeptide_epimerase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR013342, Mandelate_racemase_C IPR013341, Mandelate_racemase_N_dom |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AEEP_THEMA | |
Accessioni | Q9WXM1Primary (citable) accession number: Q9WXM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 24, 2009 |
Last sequence update: | November 1, 1999 | |
Last modified: | June 2, 2021 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families