UniProtKB - Q9WVS7 (MP2K5_MOUSE)
Dual specificity mitogen-activated protein kinase kinase 5
Map2k5
Functioni
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
2 PublicationsCatalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 195 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 283 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 172 – 180 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: MGI
- MAP kinase kinase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- protein kinase activity Source: MGI
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein serine kinase activity Source: RHEA
- protein tyrosine kinase activity Source: UniProtKB-KW
GO - Biological processi
- cellular response to growth factor stimulus Source: MGI
- ERK5 cascade Source: MGI
- heart development Source: MGI
- MAPK cascade Source: MGI
- negative regulation of cell migration involved in sprouting angiogenesis Source: MGI
- negative regulation of chemokine (C-X-C motif) ligand 2 production Source: MGI
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- negative regulation of heterotypic cell-cell adhesion Source: MGI
- negative regulation of interleukin-8 production Source: MGI
- negative regulation of NF-kappaB transcription factor activity Source: MGI
- negative regulation of response to cytokine stimulus Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of cell growth Source: MGI
- positive regulation of epithelial cell proliferation Source: MGI
- positive regulation of MAP kinase activity Source: MGI
- positive regulation of protein metabolic process Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein phosphorylation Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Dual specificity mitogen-activated protein kinase kinase 5 (EC:2.7.12.2)Short name: MAP kinase kinase 5 Short name: MAPKK 5 Alternative name(s): MAPK/ERK kinase 5 Short name: MEK 5 |
Gene namesi | Name:Map2k5 Synonyms:Mek5, Mkk5, Prkmk5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346345, Map2k5 |
VEuPathDBi | HostDB:ENSMUSG00000058444 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 20 | R → A: Loss of MAPK7 binding; when associated with A-21. 1 Publication | 1 | |
Mutagenesisi | 21 | I → A: Loss of MAPK7 binding; when associated with A-20. 1 Publication | 1 | |
Mutagenesisi | 64 | D → A: Loss of MAP3K2/MAP3K3 binding; when associated with A-65. 1 Publication | 1 | |
Mutagenesisi | 65 | E → A: Loss of MAP3K2/MAP3K3 binding; when associated with A-64. 1 Publication | 1 | |
Mutagenesisi | 67 | G → A: Loss of MAP3K2/MAP3K3 binding; when associated with A-68. 1 Publication | 1 | |
Mutagenesisi | 68 | D → A: Loss of MAP3K2/MAP3K3 binding; when associated with A-67. 1 Publication | 1 | |
Mutagenesisi | 311 | S → A: Dominant negative form; when associated with V-315. 1 Publication | 1 | |
Mutagenesisi | 311 | S → D: Dominant active form; when associated with D-315. 1 Publication | 1 | |
Mutagenesisi | 315 | T → D: Dominant active form; when associated with D-311. 1 Publication | 1 | |
Mutagenesisi | 315 | T → V: Dominant negative form; when associated with A-311. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086384 | 1 – 448 | Dual specificity mitogen-activated protein kinase kinase 5Add BLAST | 448 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 311 | PhosphoserineBy similarity | 1 | |
Modified residuei | 315 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9WVS7 |
MaxQBi | Q9WVS7 |
PaxDbi | Q9WVS7 |
PRIDEi | Q9WVS7 |
ProteomicsDBi | 290291 [Q9WVS7-1] 290292 [Q9WVS7-2] 290293 [Q9WVS7-3] 290294 [Q9WVS7-4] |
PTM databases
iPTMneti | Q9WVS7 |
PhosphoSitePlusi | Q9WVS7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000058444, Expressed in internal carotid artery and 304 other tissues |
Genevisiblei | Q9WVS7, MM |
Interactioni
Subunit structurei
Interacts with PARD6A, MAP3K3 and MAPK7.
Forms a complex with SQSTM1 and PRKCZ or PRKCI.
2 PublicationsBinary interactionsi
Q9WVS7
With | #Exp. | IntAct |
---|---|---|
Map3k2 [Q61083] | 4 | EBI-446144,EBI-446134 |
Map3k3 [Q61084] | 15 | EBI-446144,EBI-446250 |
Nap1l1 [P28656] | 20 | EBI-446144,EBI-645055 |
Sqstm1 [Q64337] | 3 | EBI-446144,EBI-645025 |
Protein-protein interaction databases
CORUMi | Q9WVS7 |
IntActi | Q9WVS7, 11 interactors |
STRINGi | 10090.ENSMUSP00000034920 |
Miscellaneous databases
RNActi | Q9WVS7, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9WVS7 |
SMRi | Q9WVS7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9WVS7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 109 | PB1PROSITE-ProRule annotationAdd BLAST | 92 | |
Domaini | 166 – 419 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 18 – 25 | Interaction with MAPK71 Publication | 8 | |
Regioni | 64 – 68 | Interaction with MAP3K2/MAP3K32 Publications | 5 | |
Regioni | 116 – 144 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 117 – 131 | Interaction with MAPK71 PublicationAdd BLAST | 15 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 123 – 144 | Polar residuesSequence analysisAdd BLAST | 22 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0581, Eukaryota |
GeneTreei | ENSGT00940000157505 |
HOGENOMi | CLU_000288_2_0_1 |
InParanoidi | Q9WVS7 |
OMAi | ILANGQX |
OrthoDBi | 688282at2759 |
PhylomeDBi | Q9WVS7 |
TreeFami | TF106468 |
Family and domain databases
CDDi | cd06395, PB1_Map2k5, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000270, PB1_dom IPR034851, PB1_MAP2K5 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00564, PB1, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00666, PB1, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51745, PB1, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLWLALGPFC AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ
60 70 80 90 100
VLPEATTTAF EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE
110 120 130 140 150
PLQIFPRACK PPGERNIHGL KVNTRAGPSQ HTSPVVSDSL PSNSLKKSSA
160 170 180 190 200
ELRKILANGQ MNEQDIRYRD TLGHGNGGTV YKAHHVPSGK ILAVKVILLD
210 220 230 240 250
ITLELQKQIM SELEILYKCD SSYIIGFYGA FFVENRISIC TEFMDGGSLD
260 270 280 290 300
VYRKIPEHVL GRIAVAVVKG LTYLWSLKIL HRDVKPSNML VNTGGQVKLC
310 320 330 340 350
DFGVSTQLVN SIAKTYVGTN AYMAPERISG EQYGIHSDVW SLGISFMELA
360 370 380 390 400
LGRFPYPQIQ KNQGSLMPLQ LLQCIVDEDS PVLPLGEFSE PFVHFITQCM
410 420 430 440
RKQPKERPAP EELMGHPFIV QFNDGNSTVV SMWVCRALEE RRSQQGPP
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015836 | 108 – 118 | ACKPPGERNIH → GYRRGSRLREY in isoform 4. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_015837 | 119 – 448 | Missing in isoform 4. 1 PublicationAdd BLAST | 330 | |
Alternative sequenceiVSP_015838 | 183 – 186 | AHHV → LLHI in isoform 3. 1 Publication | 4 | |
Alternative sequenceiVSP_015839 | 187 – 448 | Missing in isoform 3. 1 PublicationAdd BLAST | 262 | |
Alternative sequenceiVSP_015840 | 359 – 367 | Missing in isoform 2. 1 Publication | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019374 mRNA Translation: BAA82040.1 AK020716 mRNA Translation: BAB32187.1 BC013697 mRNA Translation: AAH13697.1 BC028260 mRNA Translation: AAH28260.1 |
CCDSi | CCDS23269.1 [Q9WVS7-1] |
RefSeqi | NP_035970.1, NM_011840.2 [Q9WVS7-1] XP_006511208.1, XM_006511145.2 |
Genome annotation databases
Ensembli | ENSMUST00000034920; ENSMUSP00000034920; ENSMUSG00000058444 [Q9WVS7-1] |
GeneIDi | 23938 |
KEGGi | mmu:23938 |
UCSCi | uc009qaw.1, mouse [Q9WVS7-1] uc009qaz.1, mouse [Q9WVS7-4] uc012guv.1, mouse [Q9WVS7-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019374 mRNA Translation: BAA82040.1 AK020716 mRNA Translation: BAB32187.1 BC013697 mRNA Translation: AAH13697.1 BC028260 mRNA Translation: AAH28260.1 |
CCDSi | CCDS23269.1 [Q9WVS7-1] |
RefSeqi | NP_035970.1, NM_011840.2 [Q9WVS7-1] XP_006511208.1, XM_006511145.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WI0 | NMR | - | A | 8-107 | [»] | |
AlphaFoldDBi | Q9WVS7 | |||||
SMRi | Q9WVS7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | Q9WVS7 |
IntActi | Q9WVS7, 11 interactors |
STRINGi | 10090.ENSMUSP00000034920 |
PTM databases
iPTMneti | Q9WVS7 |
PhosphoSitePlusi | Q9WVS7 |
Proteomic databases
EPDi | Q9WVS7 |
MaxQBi | Q9WVS7 |
PaxDbi | Q9WVS7 |
PRIDEi | Q9WVS7 |
ProteomicsDBi | 290291 [Q9WVS7-1] 290292 [Q9WVS7-2] 290293 [Q9WVS7-3] 290294 [Q9WVS7-4] |
Protocols and materials databases
Antibodypediai | 26267, 601 antibodies from 37 providers |
DNASUi | 23938 |
Genome annotation databases
Ensembli | ENSMUST00000034920; ENSMUSP00000034920; ENSMUSG00000058444 [Q9WVS7-1] |
GeneIDi | 23938 |
KEGGi | mmu:23938 |
UCSCi | uc009qaw.1, mouse [Q9WVS7-1] uc009qaz.1, mouse [Q9WVS7-4] uc012guv.1, mouse [Q9WVS7-2] |
Organism-specific databases
CTDi | 5607 |
MGIi | MGI:1346345, Map2k5 |
VEuPathDBi | HostDB:ENSMUSG00000058444 |
Phylogenomic databases
eggNOGi | KOG0581, Eukaryota |
GeneTreei | ENSGT00940000157505 |
HOGENOMi | CLU_000288_2_0_1 |
InParanoidi | Q9WVS7 |
OMAi | ILANGQX |
OrthoDBi | 688282at2759 |
PhylomeDBi | Q9WVS7 |
TreeFami | TF106468 |
Miscellaneous databases
BioGRID-ORCSi | 23938, 5 hits in 74 CRISPR screens |
ChiTaRSi | Map2k5, mouse |
EvolutionaryTracei | Q9WVS7 |
PROi | PR:Q9WVS7 |
RNActi | Q9WVS7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000058444, Expressed in internal carotid artery and 304 other tissues |
Genevisiblei | Q9WVS7, MM |
Family and domain databases
CDDi | cd06395, PB1_Map2k5, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000270, PB1_dom IPR034851, PB1_MAP2K5 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00564, PB1, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00666, PB1, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51745, PB1, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MP2K5_MOUSE | |
Accessioni | Q9WVS7Primary (citable) accession number: Q9WVS7 Secondary accession number(s): Q8CFM3, Q8K360, Q9D222 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | November 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families