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Protein

Methylthioribulose-1-phosphate dehydratase

Gene

Apip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.UniRule annotation2 Publications

Catalytic activityi

S-methyl-5-thio-D-ribulose 1-phosphate = 5-(methylthio)-2,3-dioxopentyl phosphate + H2O.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 2 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (Mri1)
  2. Methylthioribulose-1-phosphate dehydratase (Apip)
  3. Enolase-phosphatase E1 (Enoph1)
  4. Enolase-phosphatase E1 (Enoph1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (Adi1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateUniRule annotation1
Metal bindingi114Zinc; via tele nitrogenUniRule annotation1
Metal bindingi116Zinc; via tele nitrogenUniRule annotation1
Active sitei138Proton donor/acceptorUniRule annotation1
Metal bindingi194Zinc; via tele nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Apoptosis, Methionine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00875

Names & Taxonomyi

Protein namesi
Recommended name:
Methylthioribulose-1-phosphate dehydrataseUniRule annotation (EC:4.2.1.109UniRule annotation)
Short name:
MTRu-1-P dehydrataseUniRule annotation
Alternative name(s):
APAF1-interacting proteinUniRule annotation
Monocyte/macrophage protein 19
Gene namesi
Name:ApipUniRule annotation
Synonyms:Mmrp19
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1926788 Apip

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002390231 – 241Methylthioribulose-1-phosphate dehydrataseAdd BLAST241

Proteomic databases

EPDiQ9WVQ5
MaxQBiQ9WVQ5
PaxDbiQ9WVQ5
PeptideAtlasiQ9WVQ5
PRIDEiQ9WVQ5

PTM databases

iPTMnetiQ9WVQ5
PhosphoSitePlusiQ9WVQ5
SwissPalmiQ9WVQ5

Expressioni

Tissue specificityi

Expressed in skeletal muscle (at protein level).1 Publication

Inductioni

Up-regulated upon ischemia/hypoxia.1 Publication

Gene expression databases

BgeeiENSMUSG00000010911 Expressed in 250 organ(s), highest expression level in brown adipose tissue
CleanExiMM_APIP
GenevisibleiQ9WVQ5 MM

Interactioni

Subunit structurei

Homotetramer. Interacts with APAF1. May interact with CASP1.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9WVQ5, 1 interactor
MINTiQ9WVQ5
STRINGi10090.ENSMUSP00000011055

Structurei

3D structure databases

ProteinModelPortaliQ9WVQ5
SMRiQ9WVQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldolase class II family. MtnB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2631 Eukaryota
COG0235 LUCA
GeneTreeiENSGT00390000001680
HOGENOMiHOG000192424
HOVERGENiHBG080536
InParanoidiQ9WVQ5
KOiK08964
OMAiGHGLYTW
OrthoDBiEOG091G0GMC
PhylomeDBiQ9WVQ5
TreeFamiTF105632

Family and domain databases

Gene3Di3.40.225.10, 1 hit
HAMAPiMF_03116 Salvage_MtnB_euk, 1 hit
InterProiView protein in InterPro
IPR001303 Aldolase_II/adducin_N
IPR036409 Aldolase_II/adducin_N_sf
IPR017714 MethylthioRu-1-P_deHdtase_MtnB
IPR027514 Salvage_MtnB_euk
PANTHERiPTHR10640 PTHR10640, 1 hit
PfamiView protein in Pfam
PF00596 Aldolase_II, 1 hit
SMARTiView protein in SMART
SM01007 Aldolase_II, 1 hit
SUPFAMiSSF53639 SSF53639, 1 hit
TIGRFAMsiTIGR03328 salvage_mtnB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WVQ5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGCQAQGDC CSRPCGAQDK EHPRFLIPEL CKQFYHLGWV TGTGGGISLK
60 70 80 90 100
HGNEIYIAPS GVQKERIQPE DMFVCDINEQ DISGPPASKK LKKSQCTPLF
110 120 130 140 150
MNAYTMRGAG AVIHTHSKAA VMATLLFPGQ EFKITHQEMI KGIRKCTSGG
160 170 180 190 200
YYRYDDMLVV PIIENTPEEK DLKERMAHAM NEYPDSCAVL VRRHGVYVWG
210 220 230 240
ETWEKAKTMC ECYDYLFDIA VSMKKMGLDP TQLPVGENGI V
Length:241
Mass (Da):26,949
Last modified:November 1, 1999 - v1
Checksum:i4366CF4AD2239DB8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11C → S in BAC36968 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028863 mRNA Translation: BAA78906.1
AK077705 mRNA Translation: BAC36968.1
AK145450 mRNA Translation: BAE26445.1
BC028434 mRNA Translation: AAH28434.1
CCDSiCCDS16474.1
RefSeqiNP_062709.3, NM_019735.4
UniGeneiMm.24772

Genome annotation databases

EnsembliENSMUST00000011055; ENSMUSP00000011055; ENSMUSG00000010911
GeneIDi56369
KEGGimmu:56369
UCSCiuc008lin.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028863 mRNA Translation: BAA78906.1
AK077705 mRNA Translation: BAC36968.1
AK145450 mRNA Translation: BAE26445.1
BC028434 mRNA Translation: AAH28434.1
CCDSiCCDS16474.1
RefSeqiNP_062709.3, NM_019735.4
UniGeneiMm.24772

3D structure databases

ProteinModelPortaliQ9WVQ5
SMRiQ9WVQ5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WVQ5, 1 interactor
MINTiQ9WVQ5
STRINGi10090.ENSMUSP00000011055

PTM databases

iPTMnetiQ9WVQ5
PhosphoSitePlusiQ9WVQ5
SwissPalmiQ9WVQ5

Proteomic databases

EPDiQ9WVQ5
MaxQBiQ9WVQ5
PaxDbiQ9WVQ5
PeptideAtlasiQ9WVQ5
PRIDEiQ9WVQ5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000011055; ENSMUSP00000011055; ENSMUSG00000010911
GeneIDi56369
KEGGimmu:56369
UCSCiuc008lin.2 mouse

Organism-specific databases

CTDi51074
MGIiMGI:1926788 Apip

Phylogenomic databases

eggNOGiKOG2631 Eukaryota
COG0235 LUCA
GeneTreeiENSGT00390000001680
HOGENOMiHOG000192424
HOVERGENiHBG080536
InParanoidiQ9WVQ5
KOiK08964
OMAiGHGLYTW
OrthoDBiEOG091G0GMC
PhylomeDBiQ9WVQ5
TreeFamiTF105632

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00875

Miscellaneous databases

ChiTaRSiApip mouse
PROiPR:Q9WVQ5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010911 Expressed in 250 organ(s), highest expression level in brown adipose tissue
CleanExiMM_APIP
GenevisibleiQ9WVQ5 MM

Family and domain databases

Gene3Di3.40.225.10, 1 hit
HAMAPiMF_03116 Salvage_MtnB_euk, 1 hit
InterProiView protein in InterPro
IPR001303 Aldolase_II/adducin_N
IPR036409 Aldolase_II/adducin_N_sf
IPR017714 MethylthioRu-1-P_deHdtase_MtnB
IPR027514 Salvage_MtnB_euk
PANTHERiPTHR10640 PTHR10640, 1 hit
PfamiView protein in Pfam
PF00596 Aldolase_II, 1 hit
SMARTiView protein in SMART
SM01007 Aldolase_II, 1 hit
SUPFAMiSSF53639 SSF53639, 1 hit
TIGRFAMsiTIGR03328 salvage_mtnB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTNB_MOUSE
AccessioniPrimary (citable) accession number: Q9WVQ5
Secondary accession number(s): Q8BP46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1999
Last modified: October 10, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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