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Protein

Exostosin-like 3

Gene

Extl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase which regulates the biosynthesis of heparan sulfate (HS). Important for both skeletal development and hematopoiesis, through the formation of HS proteoglycans (HSPGs). Required for the function of REG3A in regulating keratinocyte proliferation and differentiation (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(alpha-D-GlcNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.By similarity

Cofactori

Mn2+By similarity

Pathwayi: heparan sulfate biosynthesis

This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei696SubstrateBy similarity1
Metal bindingi745Manganese; catalyticBy similarity1
Binding sitei773SubstrateBy similarity1
Active sitei832By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00756

Protein family/group databases

CAZyiGT47 Glycosyltransferase Family 47
GT64 Glycosyltransferase Family 64

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-like 3By similarity (EC:2.4.1.223By similarity)
Alternative name(s):
Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
Multiple exostosis-like protein 3By similarity
Gene namesi
Name:Extl3Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1860765 Extl3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 51Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini52 – 918LumenalSequence analysisAdd BLAST867

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001496581 – 918Exostosin-like 3Add BLAST918

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi277N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi290N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei361PhosphoserineCombined sources1
Glycosylationi591N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi789N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi830 ↔ 878By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9WVL6
PaxDbiQ9WVL6
PeptideAtlasiQ9WVL6
PRIDEiQ9WVL6

PTM databases

iPTMnetiQ9WVL6
PhosphoSitePlusiQ9WVL6
SwissPalmiQ9WVL6

Expressioni

Gene expression databases

CleanExiMM_EXTL3

Interactioni

Subunit structurei

Interacts with REG3A.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022550

Structurei

3D structure databases

ProteinModelPortaliQ9WVL6
SMRiQ9WVL6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni722 – 727Substrate bindingBy similarity6
Regioni743 – 745Substrate bindingBy similarity3
Regioni828 – 832Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2264 Eukaryota
ENOG410XNPM LUCA
HOVERGENiHBG051526
InParanoidiQ9WVL6

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004263 Exostosin
IPR015338 EXT_C
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF03016 Exostosin, 1 hit
PF09258 Glyco_transf_64, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WVL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGYTMLRNG GVGNGGQTCM LRWSNRIRLT WLSFTLFIIL VFFPLIAHYY
60 70 80 90 100
LTTLDEADEA GKRIFGPRAG SELCEVKHVL DLCRIRESVS EELLQLEAKR
110 120 130 140 150
QELNSEIAKL NLKIEACKKS IENAKQDLLQ LKNVISQTEH SYKELMAQNQ
160 170 180 190 200
PKLSLPIRLL PEKDDAGLPP PKVTRGCRLH NCFDYSRCPL TSGFPVYVYD
210 220 230 240 250
SDQFAFGSYL DPLVKQAFQA TVRANVYVTE NAAIACLYVV LVGEMQEPTV
260 270 280 290 300
LRPADLEKQL FSLPHWRTDG HNHVIINLSR KSDTQNLLYN VSTGRHVAQS
310 320 330 340 350
TFYAAQYRAG FDLVVSPLVH AMSEPNFMEI PPQVPVKRKY LFTFQGEKIE
360 370 380 390 400
SLRSSLQEAR SFEEEMEGDP PADYDDRIIA TLKAVQDSKL DQVLVEFTCK
410 420 430 440 450
NQPKPSLPTE WALCGEREDR LELLKLSTFA LIITPGDPHL LISSGCATRL
460 470 480 490 500
FEALEVGAVP VVLGEQVQLP YHDMLQWNEA ALVVPKPRVT EVHFLLRSLS
510 520 530 540 550
DSDLLAMRRQ GRFLWETYFS TADSIFNTVL AMIRTRIQIP AAPIREEVAA
560 570 580 590 600
EIPHRSGKAA GTDPNMADNG DLDLGPVETE PPYASPKYLR NFTLTVTDCY
610 620 630 640 650
RGWNSAPGPF HLFPHTPFDP VLPSEAKFLG SGTGFRPIGG GAGGSGKEFQ
660 670 680 690 700
AALGGNVQRE QFTVVMLTYE REEVLMNSLE RLNGLPYLNK VVVVWNSPKL
710 720 730 740 750
PSEDLLWPDI GVPIMVVRTE KNSLNNRFLP WNEIETEAIL SIDDDAHLRH
760 770 780 790 800
DEIMFGFRVW REARDRIVGF PGRYHAWDIP HQSWLYNSNY SCELSMVLTG
810 820 830 840 850
AAFFHKYYAY LYSYVMPQAI RDMVDEYINC EDIAMNFLVS HITRKPPIKV
860 870 880 890 900
TSRWTFRCPG CPQALSHDDS HFHERHKCIN FFVKVYGYMP LLYTQFRVDS
910
VLFKTRLPHD KTKCFKFI
Length:918
Mass (Da):104,474
Last modified:July 27, 2011 - v2
Checksum:i5A881B9BFCCB8115
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302F → L in AAD42040 (Ref. 1) Curated1
Sequence conflicti439H → R in AAD42040 (Ref. 1) Curated1
Sequence conflicti609P → R in AAD42040 (Ref. 1) Curated1
Sequence conflicti758R → W in AAD42040 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083550 mRNA Translation: AAD42040.1
AC155172 Genomic DNA No translation available.
UniGeneiMm.103748

Similar proteinsi

Entry informationi

Entry nameiEXTL3_MOUSE
AccessioniPrimary (citable) accession number: Q9WVL6
Secondary accession number(s): E9QKN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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