UniProtKB - Q9WVL4 (GRK1_MOUSE)
Rhodopsin kinase GRK1
Grk1
Functioni
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed:10097103, PubMed:10704496, PubMed:26427409).
This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (PubMed:10097103, PubMed:10704496).
May play a role in the maintenance of the outer nuclear layer in the retina (PubMed:26427409).
3 PublicationsCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 219 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 317 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 196 – 204 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- G protein-coupled receptor kinase activity Source: InterPro
- protein kinase activity Source: GO_Central
- rhodopsin kinase activity Source: UniProtKB
GO - Biological processi
- negative regulation of apoptotic process Source: MGI
- photoreceptor cell morphogenesis Source: MGI
- positive regulation of phosphorylation Source: MGI
- post-embryonic retina morphogenesis in camera-type eye Source: MGI
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: GO_Central
- regulation of rhodopsin mediated signaling pathway Source: UniProtKB
- regulation of signal transduction Source: GO_Central
- response to light stimulus Source: MGI
- signal transduction Source: InterPro
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Sensory transduction, Vision |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade |
Names & Taxonomyi
Protein namesi | Recommended name: Rhodopsin kinase GRK1 (EC:2.7.11.141 Publication)Short name: RK Alternative name(s): G protein-coupled receptor kinase 1Imported |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1345146, Grk1 |
Subcellular locationi
Other locations
- Membrane By similarity; Lipid-anchor By similarity
- photoreceptor outer segment 1 Publication
Note: Subcellular location is not affected by light or dark conditions.1 Publication
Other locations
- cytoplasm Source: GO_Central
- membrane Source: UniProtKB-SubCell
- photoreceptor outer segment Source: MGI
Keywords - Cellular componenti
Cell projection, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 363 | L → P: Defects in photoreceptor light response and loss of photoreceptors by one year of age. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000024377 | 1 – 561 | Rhodopsin kinase GRK1Add BLAST | 561 | |
PropeptideiPRO_0000024378 | 562 – 564 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 5 | PhosphoserineBy similarity | 1 | |
Modified residuei | 8 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 21 | Phosphoserine; by PKA and autocatalysis1 Publication | 1 | |
Modified residuei | 491 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 492 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 561 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 561 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
MaxQBi | Q9WVL4 |
PaxDbi | Q9WVL4 |
PRIDEi | Q9WVL4 |
ProteomicsDBi | 255155 |
PTM databases
iPTMneti | Q9WVL4 |
PhosphoSitePlusi | Q9WVL4 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts (via N-terminus) with RCVRN (via C-terminus); the interaction is Ca2+-dependent (By similarity).
Interacts (when prenylated) with PDE6D; this promotes release from membranes (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca2+-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033827 |
Miscellaneous databases
RNActi | Q9WVL4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 58 – 175 | RGSPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 190 – 455 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 266 | |
Domaini | 456 – 521 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 66 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 189 | N-terminalAdd BLAST | 189 | |
Regioni | 1 – 15 | Interaction with RCVRNBy similarityAdd BLAST | 15 | |
Regioni | 456 – 564 | C-terminalAdd BLAST | 109 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0986, Eukaryota |
InParanoidi | Q9WVL4 |
PhylomeDBi | Q9WVL4 |
Family and domain databases
CDDi | cd05608, STKc_GRK1, 1 hit |
Gene3Di | 1.10.167.10, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR000239, GPCR_kinase IPR032965, GRK1 IPR037716, GRK1_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR016137, RGS IPR036305, RGS_sf IPR044926, RGS_subdomain_2 IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR24355:SF11, PTHR24355:SF11, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00615, RGS, 1 hit |
PRINTSi | PR00717, GPCRKINASE |
SMARTi | View protein in SMART SM00315, RGS, 1 hit SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50132, RGS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MDFGSLETVV ANSAFIAARG SFDGSSTPSS RDKKYLAKLR LPPLSKCEGL
60 70 80 90 100
RDNLSLEFES LCSEQPIGKR LFQQFLKTDE RHVPALELWK DIEDYDTADD
110 120 130 140 150
DLRPQKAQAI LAEYLDPQGT LFCNFLDQGM VTRVKEGPTG SQDGLFQPLL
160 170 180 190 200
QATMEHLSQA PFQEYLESLY FLRFLQWKWL EAQPIGEDWF LDFRVLGKGG
210 220 230 240 250
FGEVSACQMK ATGKMYACKK LNKKRLKKKK GYQGAIVEKR ILTKVHSRFI
260 270 280 290 300
VSLAYAFETK TDLCLVMTIM NGGDVRYHIY NVDEDNPGFS EPRAIYYTAQ
310 320 330 340 350
IISGLEHLHQ RRIVYRDLKP ENVLLDNDGN IRISDLGLAV ELKEGQNKTK
360 370 380 390 400
GYAGTPGFMA PELLRGEEYD FSVDYFALGV TLYEMIAARG PFRARGEKVE
410 420 430 440 450
NKELKQRIIS EPVKYPEKFS QASKDFCEQL LEKDPEKRLG FRDGTCDALR
460 470 480 490 500
ANVLFKDISW RQLEAGMLIP PFIPDSRTVY AKNIQDVGAF STVKGVVFDK
510 520 530 540 550
ADTEFFQEFA SGNCSIPWQE EMIETGLFGD LNVWRADGQM PDDMKGITTE
560
EAAPTAKSGM CLIS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3X905 | G3X905_MOUSE | G protein-coupled receptor kinase | Grk1 | 564 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF085240 mRNA Translation: AAD40189.1 |
CCDSi | CCDS22112.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF085240 mRNA Translation: AAD40189.1 |
CCDSi | CCDS22112.1 |
3D structure databases
AlphaFoldDBi | Q9WVL4 |
SMRi | Q9WVL4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033827 |
PTM databases
iPTMneti | Q9WVL4 |
PhosphoSitePlusi | Q9WVL4 |
Proteomic databases
MaxQBi | Q9WVL4 |
PaxDbi | Q9WVL4 |
PRIDEi | Q9WVL4 |
ProteomicsDBi | 255155 |
Organism-specific databases
MGIi | MGI:1345146, Grk1 |
Phylogenomic databases
eggNOGi | KOG0986, Eukaryota |
InParanoidi | Q9WVL4 |
PhylomeDBi | Q9WVL4 |
Enzyme and pathway databases
Reactomei | R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade |
Miscellaneous databases
ChiTaRSi | Grk1, mouse |
PROi | PR:Q9WVL4 |
RNActi | Q9WVL4, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd05608, STKc_GRK1, 1 hit |
Gene3Di | 1.10.167.10, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR000239, GPCR_kinase IPR032965, GRK1 IPR037716, GRK1_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR016137, RGS IPR036305, RGS_sf IPR044926, RGS_subdomain_2 IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR24355:SF11, PTHR24355:SF11, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00615, RGS, 1 hit |
PRINTSi | PR00717, GPCRKINASE |
SMARTi | View protein in SMART SM00315, RGS, 1 hit SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50132, RGS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GRK1_MOUSE | |
Accessioni | Q9WVL4Primary (citable) accession number: Q9WVL4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families