UniProtKB - Q9WVL2 (STAT2_MOUSE)
Signal transducer and activator of transcription 2
Stat2
Functioni
Signal transducer and activator of transcription that mediates signaling by type I interferons (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In addition, has also a negative feedback regulatory role in the type I interferon signaling by recruiting USP18 to the type I IFN receptor subunit IFNAR2 thereby mitigating the response to type I IFNs. Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively.
By similarityGO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- identical protein binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- ubiquitin-like protein ligase binding Source: MGI
GO - Biological processi
- cytokine-mediated signaling pathway Source: GO_Central
- defense response Source: GO_Central
- defense response to virus Source: UniProtKB
- negative regulation of type I interferon-mediated signaling pathway Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- receptor signaling pathway via JAK-STAT Source: GO_Central
- regulation of cell population proliferation Source: GO_Central
- regulation of mitochondrial fission Source: UniProtKB
- regulation of protein phosphorylation Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to peptide hormone Source: GO_Central
- type I interferon signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-8854691, Interleukin-20 family signaling R-MMU-909733, Interferon alpha/beta signaling R-MMU-912694, Regulation of IFNA signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Signal transducer and activator of transcription 2 |
Gene namesi | Name:Stat2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103039, Stat2 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- ISGF3 complex Source: MGI
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000182414 | 1 – 923 | Signal transducer and activator of transcription 2Add BLAST | 923 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 284 | PhosphoserineBy similarity | 1 | |
Modified residuei | 689 | Phosphotyrosine; by JAKBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9WVL2 |
MaxQBi | Q9WVL2 |
PaxDbi | Q9WVL2 |
PeptideAtlasi | Q9WVL2 |
PRIDEi | Q9WVL2 |
ProteomicsDBi | 258658 |
PTM databases
iPTMneti | Q9WVL2 |
PhosphoSitePlusi | Q9WVL2 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation. The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9; interacts with IRF9 in the cytoplasm (PubMed:17332413).
Interacts with CRSP2 and CRSP6 (By similarity). Can form a homodimer upon IFN-alpha induced phosphorylation.
Interacts with IFNAR1 and IFNAR2; the interaction is direct.
Interacts with ARL2BP (PubMed:18234692).
Interacts with E3 ubiquitin ligase DCST1; the interaction results in STAT2 ubiquitin-mediated proteasomal degradation (By similarity).
Interacts with USP18; the interaction is direct and allows the recruitment of USP18 to IFNAR2 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- ubiquitin-like protein ligase binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000100872 |
Miscellaneous databases
RNActi | Q9WVL2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 571 – 666 | SH2PROSITE-ProRule annotationAdd BLAST | 96 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 137 – 571 | Mediates interaction with USP18By similarityAdd BLAST | 435 |
Sequence similaritiesi
Keywords - Domaini
SH2 domainPhylogenomic databases
eggNOGi | KOG3667, Eukaryota |
InParanoidi | Q9WVL2 |
PhylomeDBi | Q9WVL2 |
Family and domain databases
CDDi | cd10373, SH2_STAT2, 1 hit |
Gene3Di | 1.10.532.10, 1 hit 2.60.40.630, 1 hit 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR008967, p53-like_TF_DNA-bd IPR000980, SH2 IPR036860, SH2_dom_sf IPR001217, STAT IPR022756, STAT2_C IPR035854, STAT2_SH2 IPR036535, STAT_N_sf IPR013800, STAT_TF_alpha IPR015988, STAT_TF_coiled-coil IPR013801, STAT_TF_DNA-bd IPR012345, STAT_TF_DNA-bd_N IPR013799, STAT_TF_prot_interaction |
PANTHERi | PTHR11801, PTHR11801, 1 hit |
Pfami | View protein in Pfam PF00017, SH2, 1 hit PF12188, STAT2_C, 1 hit PF01017, STAT_alpha, 1 hit PF02864, STAT_bind, 1 hit PF02865, STAT_int, 1 hit |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00964, STAT_int, 1 hit |
SUPFAMi | SSF47655, SSF47655, 1 hit SSF48092, SSF48092, 1 hit SSF49417, SSF49417, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAQWEMLQNL DSPFLDQLHQ VYSQSFLPMD FRQHLASWIE DQNWREAALE
60 70 80 90 100
SDDAKANMLY FSILDQLNQW DHYSSDPKSL LLQHNLRKFS RDIQPFPNGP
110 120 130 140 150
SQLAEMIFNL LLEEQRILIQ AQRAQEVQPP PAPEAVVESQ QLEIENRIQG
160 170 180 190 200
LHVDIEFLVR SIRQLKDEQD VFSFRYTVFS LKKTSSSDPH QSQQAHVVQA
210 220 230 240 250
TANKVDRMRK EVLDISKGLV GRLTTLVDLL LPKLDEWKVQ QQKSCIGAPP
260 270 280 290 300
PVKSAAEQLE QWLTAGAKFL FHLRQLLKQL KEMSCLRYQG DMFAKGVDLR
310 320 330 340 350
NAQVMELLQR LLQRSFVVET QPCMPQTLHR PLILKTGNKF TVRTRLLVRL
360 370 380 390 400
QEGSESLKAE VSVDRNSDLP GFRKFNILTS NQKTLTPEKG QRQGLIWDFG
410 420 430 440 450
FLTLVEQRAV GAGKGNNKGP LAVTEELHVI SFVVEYTYQG LKMKLQTDTL
460 470 480 490 500
PVVIISNMNQ LSFAWASILW FNMLSPNPKN QQFFCQAPKA PWSLLGPVLS
510 520 530 540 550
WQFSSYVARG LDSEQLGMLR TKLFGKSCKM EDALLSWVDF CKRESPPGKI
560 570 580 590 600
PFWTWLDKIL ELVHDHLKDL WKDGRIMGFV SRNQERRLLK KMLSGTFLLR
610 620 630 640 650
FSETSEGGIT CSWVEHQDDH KVEIYSVQPY TKEVLQSLPL TEIIRHYQVL
660 670 680 690 700
AEENIPENPL RFLYPRIPRD EAFGCYYQEK VNLEEQEEYL KHKLIVISNR
710 720 730 740 750
QVDELQQPLE LKQDSESLEV NAELLLAHDQ ELPLMMQTGL VLGTELKVDP
760 770 780 790 800
ILSTAPQVLL EPAPQVLLEP APQVLLEPAP QVLLEPAPQV LLEPAPQVLL
810 820 830 840 850
EPAPQVLLEP APQVQLEPAP QVLLELAPQV LLEPAPQVLL ELAPQVQLEP
860 870 880 890 900
AHLLQQPSES DLPEDLQQIS VEDLKKLSNP STEYITTNEN PMLAGESSGD
910 920
ETSIPYHSHF DADGLLGWTL DTF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QJX9 | E9QJX9_MOUSE | Signal transducer and activator of ... | Stat2 | 923 | Annotation score: | ||
Q9QXJ2 | Q9QXJ2_MOUSE | Signal transducer and activator of ... | Stat2 | 922 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 596 | T → A in AAB36228 (PubMed:8601453).Curated | 1 | |
Sequence conflicti | 596 | T → A in AAB36231 (PubMed:8601453).Curated | 1 | |
Sequence conflicti | 620 | H → D in AAB36228 (PubMed:8601453).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF088862 mRNA Translation: AAD38329.1 S81342 Genomic DNA Translation: AAB36228.2 S81342 Genomic DNA Translation: AAB36230.1 Sequence problems. S81342 Genomic DNA Translation: AAB36231.1 Sequence problems. |
PIRi | S63681 S63682 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF088862 mRNA Translation: AAD38329.1 S81342 Genomic DNA Translation: AAB36228.2 S81342 Genomic DNA Translation: AAB36230.1 Sequence problems. S81342 Genomic DNA Translation: AAB36231.1 Sequence problems. |
PIRi | S63681 S63682 |
3D structure databases
AlphaFoldDBi | Q9WVL2 |
SMRi | Q9WVL2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000100872 |
PTM databases
iPTMneti | Q9WVL2 |
PhosphoSitePlusi | Q9WVL2 |
Proteomic databases
EPDi | Q9WVL2 |
MaxQBi | Q9WVL2 |
PaxDbi | Q9WVL2 |
PeptideAtlasi | Q9WVL2 |
PRIDEi | Q9WVL2 |
ProteomicsDBi | 258658 |
Organism-specific databases
MGIi | MGI:103039, Stat2 |
Phylogenomic databases
eggNOGi | KOG3667, Eukaryota |
InParanoidi | Q9WVL2 |
PhylomeDBi | Q9WVL2 |
Enzyme and pathway databases
Reactomei | R-MMU-8854691, Interleukin-20 family signaling R-MMU-909733, Interferon alpha/beta signaling R-MMU-912694, Regulation of IFNA signaling |
Miscellaneous databases
ChiTaRSi | Stat2, mouse |
PROi | PR:Q9WVL2 |
RNActi | Q9WVL2, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd10373, SH2_STAT2, 1 hit |
Gene3Di | 1.10.532.10, 1 hit 2.60.40.630, 1 hit 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR008967, p53-like_TF_DNA-bd IPR000980, SH2 IPR036860, SH2_dom_sf IPR001217, STAT IPR022756, STAT2_C IPR035854, STAT2_SH2 IPR036535, STAT_N_sf IPR013800, STAT_TF_alpha IPR015988, STAT_TF_coiled-coil IPR013801, STAT_TF_DNA-bd IPR012345, STAT_TF_DNA-bd_N IPR013799, STAT_TF_prot_interaction |
PANTHERi | PTHR11801, PTHR11801, 1 hit |
Pfami | View protein in Pfam PF00017, SH2, 1 hit PF12188, STAT2_C, 1 hit PF01017, STAT_alpha, 1 hit PF02864, STAT_bind, 1 hit PF02865, STAT_int, 1 hit |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00964, STAT_int, 1 hit |
SUPFAMi | SSF47655, SSF47655, 1 hit SSF48092, SSF48092, 1 hit SSF49417, SSF49417, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | STAT2_MOUSE | |
Accessioni | Q9WVL2Primary (citable) accession number: Q9WVL2 Secondary accession number(s): Q64188, Q64189, Q64250 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families