Ehd1

Functionⁱ

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes (By similarity). Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (PubMed:15930129, PubMed:20159556). Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity). Plays a role in myoblast fusion (PubMed:21177873). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (PubMed:24373286). Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis (By similarity).By similarity4 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	220	ATPBy similarity		1
Binding siteⁱ	258	ATPBy similarity		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	65 – 72	ATPBy similarity		8
Calcium bindingⁱ	489 – 500	PROSITE-ProRule annotationBy similarityAdd BLAST		12

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
calcium ion binding Source: InterPro
GTP binding Source: InterPro
identical protein binding Source: MGI
Rab GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to nerve growth factor stimulus Source: UniProtKB
cholesterol homeostasis
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 17451652
cilium assembly
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 25686250
endocytic recycling Source: UniProtKB
endocytosis
Source: MGI
Inferred from direct assayⁱ
- 15247266
endosomal transport
Source: MGI
Inferred from direct assayⁱ
- 11389441
intracellular protein transport Source: UniProtKB
low-density lipoprotein particle clearance
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 17451652
neuron projection development Source: UniProtKB
positive regulation of cholesterol storage
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 17451652
positive regulation of endocytic recycling
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21177873
positive regulation of myoblast fusion
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21177873
positive regulation of neuron projection development Source: MGI
protein homooligomerization Source: MGI
protein localization to cilium Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cilium biogenesis/degradation, Protein transport, Transport
Ligand	ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Reactomeⁱ

R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: EH domain-containing protein 1Curated Alternative name(s): PAST homolog 1Curated Short name: mPAST1Imported
Gene namesⁱ	Name:Ehd1Imported Synonyms:Past1Imported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 19

Organism-specific databases

MGIⁱ	MGI:1341878 Ehd1

Keywords - Cellular componentⁱ

Cell membrane, Cell projection, Cilium, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	65	G → R: Abolishes interaction with PACSIN2. 1 Publication		1
Mutagenesisⁱ	485	W → A: Abolishes interaction with PACSIN2. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000146110	1 – 534	EH domain-containing protein 1Add BLAST		534

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity	1
Modified residueⁱ	355	PhosphoserineCombined sources	1
Modified residueⁱ	456	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q9WVK4
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9WVK4
PaxDbⁱ	Q9WVK4
PRIDEⁱ	Q9WVK4

PTM databases

iPTMnetⁱ	Q9WVK4
PhosphoSitePlusⁱ	Q9WVK4
SwissPalmⁱ	Q9WVK4

Expressionⁱ

Tissue specificityⁱ

Highly expressed in testis. Also expressed in kidney, heart, intestine, and brain.1 Publication

Developmental stageⁱ

Expression is already noted at day 9.5 in the limb buds and pharyngeal arches and at day 10.5 in sclerotomes, at various elements of the branchial apparatus (mandible and hyoid), and in the occipital region. At day 15.5 expression peaks in cartilage, preceding hypertrophy and ossification, and at day 17.5 there is no expression in the bones.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000024772 Expressed in 285 organ(s), highest expression level in ascending aorta
CleanExⁱ	MM_EHD1
ExpressionAtlasⁱ	Q9WVK4 baseline and differential
Genevisibleⁱ	Q9WVK4 MM

Interactionⁱ

Subunit structureⁱ

Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4, ATP-binding is required for heterooligomerization (By similarity). Interacts (via EH domain) with MICALL1 (via NPF1 motif); the interaction is direct and recruits EHD1 to membranes (PubMed:23572513). Interacts with RAB35; the interaction is indirect through MICALL1 and recruits EHD1 to membranes (PubMed:23572513). Interacts (via EH domain) with PACSIN2 (via NPF motifs); regulates localization to tubular recycling endosome membranes (PubMed:15930129). Interacts with PACSIN1 (PubMed:15930129). Interacts with RAB8A (By similarity). Interacts with FER1L5 (via second C2 domain) (PubMed:21177873). Interacts with MYOF (PubMed:21177873). Interacts with ZFYVE20 (By similarity). Interacts (via EH domain) with RAB11FIP2 (By similarity).By similarity3 Publications

GO - Molecular functionⁱ

identical protein binding Source: MGI
Rab GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	199408, 2 interactors
Database of interacting proteins More...DIPⁱ	DIP-59491N
IntActⁱ	Q9WVK4, 8 interactors
Molecular INTeraction database More...MINTⁱ	Q9WVK4
STRINGⁱ	10090.ENSMUSP00000025684

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9WVK4
SMRⁱ	Q9WVK4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	55 – 286	Dynamin-type GPROSITE-ProRule annotationAdd BLAST	232
Domainⁱ	444 – 532	EHPROSITE-ProRule annotationAdd BLAST	89
Domainⁱ	476 – 511	EF-handPROSITE-ProRule annotationAdd BLAST	36

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	65 – 72	G1 motifPROSITE-ProRule annotation	8
Regionⁱ	91 – 92	G2 motifPROSITE-ProRule annotation	2
Regionⁱ	153 – 156	G3 motifPROSITE-ProRule annotation	4
Regionⁱ	219 – 222	G4 motifPROSITE-ProRule annotation	4
Regionⁱ	243	G5 motifPROSITE-ProRule annotation	1

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	198 – 227	Sequence analysisAdd BLAST		30

Domainⁱ

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.1 Publication

Sequence similaritiesⁱ

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	KOG1954 Eukaryota ENOG410XYGB LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118985
HOVERGENⁱ	HBG018183
InParanoidⁱ	Q9WVK4
KEGG Orthology (KO) More...KOⁱ	K12483
OMAⁱ	RFMCAQM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G04KA
PhylomeDBⁱ	Q9WVK4
TreeFamⁱ	TF314429

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00052 EH, 1 hit
InterProⁱ	View protein in InterPro IPR022812 Dynamin_SF IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR000261 EH_dom IPR031692 EHD_N IPR030381 G_DYNAMIN_dom IPR027417 P-loop_NTPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00350 Dynamin_N, 1 hit PF12763 EF-hand_4, 1 hit PF16880 EHD_N, 1 hit
SMARTⁱ	View protein in SMART SM00027 EH, 1 hit
SUPFAMⁱ	SSF47473 SSF47473, 1 hit SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 1 hit PS50222 EF_HAND_2, 1 hit PS50031 EH, 1 hit PS51718 G_DYNAMIN_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9WVK4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL 
        60         70         80         90        100
EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV 
       110        120        130        140        150
MHGPTEGVVP GNALVVDPRR PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS 
       160        170        180        190        200
IIDTPGILSG EKQRISRGYD FAAVLEWFAE RVDRIILLFD AHKLDISDEF 
       210        220        230        240        250
SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL GKIINTPEVV 
       260        270        280        290        300
RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR 
       310        320        330        340        350
ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISS 
       360        370        380        390        400
GDFPSLRKMQ ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE 
       410        420        430        440        450
ESLMPSQAVK GGAFDGTMNG PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE 
       460        470        480        490        500
IFYTLSPVNG KITGANAKKE MVKSKLPNTV LGKIWKLADV DKDGLLDDEE 
       510        520        530 
FALANHLIKV KLEGHELPAD LPPHLIPPSK RRHE

Length:534

Mass (Da):60,603

Last modified:November 1, 1999 - v1

Checksum:ⁱ35502055BC6164F7

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF099186 mRNA Translation: AAD45423.1 AF173156 mRNA Translation: AAF24223.1 AK012896 mRNA Translation: BAB28540.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS29501.1
NCBI Reference Sequences More...RefSeqⁱ	NP_034249.1, NM_010119.5
UniGeneⁱ	Mm.30169

Genome annotation databases

Ensemblⁱ	ENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772 ENSMUST00000202020; ENSMUSP00000144036; ENSMUSG00000107039
GeneIDⁱ	13660
KEGGⁱ	mmu:13660
UCSC genome browser More...UCSCⁱ	uc008ghz.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9WVK4	EH domain-containing protein 1		RAT		534	UniRef100_Q9WVK4
EH-domain containing 1 (Fragment)		MOUSE		556
EH-domain containing 1		MOUSE		534
Uncharacterized protein (Fragment)		MOUSE		485

Protein	Similar proteins		Species	Score	Length	Source
Q9WVK4	EH domain-containing protein 1	)	HUMAN		534	UniRef90_Q9H4M9
EH domain-containing protein 1		PONAB		534
EH domain-containing protein 1		RAT		534
EH domain-containing protein 1		BOVIN		534
Uncharacterized protein		COLAP		556
+180

Protein	Similar proteins		Species	Score	Length	Source
Q9WVK4	EH domain-containing protein 1	)	HUMAN		534	UniRef50_Q9H4M9
EH domain-containing protein 1		PONAB		534
EH domain-containing protein 1		BOVIN		534
EH domain-containing protein 1		RAT		534
EH domain-containing protein 4	)	MOUSE		541
+312

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF099186 mRNA Translation: AAD45423.1 AF173156 mRNA Translation: AAF24223.1 AK012896 mRNA Translation: BAB28540.1
CCDSⁱ	CCDS29501.1
RefSeqⁱ	NP_034249.1, NM_010119.5
UniGeneⁱ	Mm.30169

3D structure databases

ProteinModelPortalⁱ	Q9WVK4
SMRⁱ	Q9WVK4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	199408, 2 interactors
DIPⁱ	DIP-59491N
IntActⁱ	Q9WVK4, 8 interactors
MINTⁱ	Q9WVK4
STRINGⁱ	10090.ENSMUSP00000025684

PTM databases

iPTMnetⁱ	Q9WVK4
PhosphoSitePlusⁱ	Q9WVK4
SwissPalmⁱ	Q9WVK4

Proteomic databases

EPDⁱ	Q9WVK4
MaxQBⁱ	Q9WVK4
PaxDbⁱ	Q9WVK4
PRIDEⁱ	Q9WVK4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772 ENSMUST00000202020; ENSMUSP00000144036; ENSMUSG00000107039
GeneIDⁱ	13660
KEGGⁱ	mmu:13660
UCSCⁱ	uc008ghz.1 mouse

Organism-specific databases

CTDⁱ	10938
MGIⁱ	MGI:1341878 Ehd1

Phylogenomic databases

eggNOGⁱ	KOG1954 Eukaryota ENOG410XYGB LUCA
GeneTreeⁱ	ENSGT00760000118985
HOVERGENⁱ	HBG018183
InParanoidⁱ	Q9WVK4
KOⁱ	K12483
OMAⁱ	RFMCAQM
OrthoDBⁱ	EOG091G04KA
PhylomeDBⁱ	Q9WVK4
TreeFamⁱ	TF314429

Enzyme and pathway databases

Reactomeⁱ	R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Reactomeⁱ

R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

ChiTaRSⁱ	Ehd1 mouse
Protein Ontology More...PROⁱ	PR:Q9WVK4
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000024772 Expressed in 285 organ(s), highest expression level in ascending aorta
CleanExⁱ	MM_EHD1
ExpressionAtlasⁱ	Q9WVK4 baseline and differential
Genevisibleⁱ	Q9WVK4 MM

Family and domain databases

CDDⁱ	cd00052 EH, 1 hit
InterProⁱ	View protein in InterPro IPR022812 Dynamin_SF IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR000261 EH_dom IPR031692 EHD_N IPR030381 G_DYNAMIN_dom IPR027417 P-loop_NTPase
Pfamⁱ	View protein in Pfam PF00350 Dynamin_N, 1 hit PF12763 EF-hand_4, 1 hit PF16880 EHD_N, 1 hit
SMARTⁱ	View protein in SMART SM00027 EH, 1 hit
SUPFAMⁱ	SSF47473 SSF47473, 1 hit SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 1 hit PS50222 EF_HAND_2, 1 hit PS50031 EH, 1 hit PS51718 G_DYNAMIN_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	EHD1_MOUSE
Accessionⁱ	Q9WVK4Primary (citable) accession number: Q9WVK4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
	Last sequence update:	November 1, 1999
	Last modified:	September 12, 2018
	This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9WVK4 (EHD1_MOUSE)

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EH domain-containing protein 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endosome

Plasma membrane

Other locations

Endosome

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ