UniProtKB - Q9WVK4 (EHD1_MOUSE)
EH domain-containing protein 1
Ehd1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 220 | ATPBy similarity | 1 | |
Binding sitei | 258 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 65 – 72 | ATPBy similarity | 8 | |
Calcium bindingi | 489 – 500 | PROSITE-ProRule annotationBy similarityAdd BLAST | 12 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium ion binding Source: InterPro
- GTP binding Source: InterPro
- identical protein binding Source: MGI
- Rab GTPase binding Source: MGI
GO - Biological processi
- cellular response to nerve growth factor stimulus Source: UniProtKB
- cholesterol homeostasis Source: BHF-UCL
- cilium assembly Source: UniProtKB
- endocytic recycling Source: UniProtKB
- endocytosis Source: MGI
- endosomal transport Source: MGI
- intracellular protein transport Source: UniProtKB
- low-density lipoprotein particle clearance Source: BHF-UCL
- neuron projection development Source: UniProtKB
- positive regulation of cholesterol storage Source: BHF-UCL
- positive regulation of endocytic recycling Source: UniProtKB
- positive regulation of myoblast fusion Source: UniProtKB
- positive regulation of neuron projection development Source: MGI
- protein homooligomerization Source: MGI
- protein localization to cilium Source: UniProtKB
- protein localization to plasma membrane Source: GO_Central
Keywordsi
Biological process | Cilium biogenesis/degradation, Protein transport, Transport |
Ligand | ATP-binding, Calcium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: EH domain-containing protein 1CuratedAlternative name(s): PAST homolog 1Curated Short name: mPAST1Imported |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1341878, Ehd1 |
Subcellular locationi
Endosome
- Recycling endosome membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
- Early endosome membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein Curated; Cytoplasmic side Curated
- cilium membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
Other locations
- Cytoplasmic vesicle 1 Publication
Note: Preferentially associates with tubular recycling endosomes (By similarity). Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes (PubMed:21177873). Localizes to the ciliary pocket from where the cilium protrudes (By similarity). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals.By similarity2 Publications
Endosome
- early endosome Source: MGI
- early endosome membrane Source: UniProtKB
- endosome Source: MGI
- endosome membrane Source: UniProtKB
- recycling endosome membrane Source: UniProtKB
Plasma Membrane
- ciliary pocket membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- endocytic vesicle Source: MGI
- intracellular membrane-bounded organelle Source: GO_Central
- lipid droplet Source: BHF-UCL
- myelin sheath Source: UniProtKB
- perinuclear region of cytoplasm Source: MGI
- platelet dense tubular network membrane Source: BHF-UCL
Keywords - Cellular componenti
Cell membrane, Cell projection, Cilium, Cytoplasmic vesicle, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 65 | G → R: Abolishes interaction with PACSIN2. 1 Publication | 1 | |
Mutagenesisi | 485 | W → A: Abolishes interaction with PACSIN2. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000146110 | 1 – 534 | EH domain-containing protein 1Add BLAST | 534 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 355 | PhosphoserineCombined sources | 1 | |
Modified residuei | 456 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9WVK4 |
jPOSTi | Q9WVK4 |
MaxQBi | Q9WVK4 |
PaxDbi | Q9WVK4 |
PRIDEi | Q9WVK4 |
PTM databases
iPTMneti | Q9WVK4 |
PhosphoSitePlusi | Q9WVK4 |
SwissPalmi | Q9WVK4 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000024772, Expressed in ascending aorta and 303 other tissues |
ExpressionAtlasi | Q9WVK4, baseline and differential |
Genevisiblei | Q9WVK4, MM |
Interactioni
Subunit structurei
Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4, ATP-binding is required for heterooligomerization (By similarity).
Interacts (via EH domain) with MICALL1 (via NPF1 motif); the interaction is direct and recruits EHD1 to membranes (PubMed:23572513).
Interacts with RAB35; the interaction is indirect through MICALL1 and recruits EHD1 to membranes (PubMed:23572513).
Interacts (via EH domain) with PACSIN2 (via NPF motifs); regulates localization to tubular recycling endosome membranes (PubMed:15930129).
Interacts with PACSIN1 (PubMed:15930129).
Interacts with RAB8A (By similarity).
Interacts with FER1L5 (via second C2 domain) (PubMed:21177873).
Interacts with MYOF (PubMed:21177873).
Interacts with ZFYVE20 (By similarity).
Interacts (via EH domain) with RAB11FIP2 (By similarity).
By similarity3 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- Rab GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199408, 14 interactors |
DIPi | DIP-59491N |
IntActi | Q9WVK4, 8 interactors |
MINTi | Q9WVK4 |
STRINGi | 10090.ENSMUSP00000025684 |
Miscellaneous databases
RNActi | Q9WVK4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 286 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 232 | |
Domaini | 444 – 532 | EHPROSITE-ProRule annotationAdd BLAST | 89 | |
Domaini | 476 – 511 | EF-handPROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 65 – 72 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 91 – 92 | G2 motifPROSITE-ProRule annotation | 2 | |
Regioni | 153 – 156 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 219 – 222 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 243 | G5 motifPROSITE-ProRule annotation | 1 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 198 – 227 | Sequence analysisAdd BLAST | 30 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1954, Eukaryota |
GeneTreei | ENSGT00940000158249 |
HOGENOMi | CLU_017595_1_1_1 |
InParanoidi | Q9WVK4 |
OMAi | MAMVRQE |
OrthoDBi | 377342at2759 |
PhylomeDBi | Q9WVK4 |
TreeFami | TF314429 |
Family and domain databases
CDDi | cd00052, EH, 1 hit |
InterProi | View protein in InterPro IPR040990, DUF5600 IPR022812, Dynamin_SF IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR000261, EH_dom IPR029951, EHD1/EHD3 IPR031692, EHD_N IPR030381, G_DYNAMIN_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR11216:SF127, PTHR11216:SF127, 1 hit |
Pfami | View protein in Pfam PF18150, DUF5600, 1 hit PF00350, Dynamin_N, 1 hit PF12763, EF-hand_4, 1 hit PF16880, EHD_N, 1 hit |
SMARTi | View protein in SMART SM00027, EH, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS50031, EH, 1 hit PS51718, G_DYNAMIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL
60 70 80 90 100
EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV
110 120 130 140 150
MHGPTEGVVP GNALVVDPRR PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS
160 170 180 190 200
IIDTPGILSG EKQRISRGYD FAAVLEWFAE RVDRIILLFD AHKLDISDEF
210 220 230 240 250
SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL GKIINTPEVV
260 270 280 290 300
RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR
310 320 330 340 350
ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISS
360 370 380 390 400
GDFPSLRKMQ ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE
410 420 430 440 450
ESLMPSQAVK GGAFDGTMNG PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE
460 470 480 490 500
IFYTLSPVNG KITGANAKKE MVKSKLPNTV LGKIWKLADV DKDGLLDDEE
510 520 530
FALANHLIKV KLEGHELPAD LPPHLIPPSK RRHE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF099186 mRNA Translation: AAD45423.1 AF173156 mRNA Translation: AAF24223.1 AK012896 mRNA Translation: BAB28540.1 |
CCDSi | CCDS29501.1 |
RefSeqi | NP_034249.1, NM_010119.5 |
Genome annotation databases
Ensembli | ENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772 ENSMUST00000202020; ENSMUSP00000144036; ENSMUSG00000107039 |
GeneIDi | 13660 |
KEGGi | mmu:13660 |
UCSCi | uc008ghz.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF099186 mRNA Translation: AAD45423.1 AF173156 mRNA Translation: AAF24223.1 AK012896 mRNA Translation: BAB28540.1 |
CCDSi | CCDS29501.1 |
RefSeqi | NP_034249.1, NM_010119.5 |
3D structure databases
BMRBi | Q9WVK4 |
SMRi | Q9WVK4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199408, 14 interactors |
DIPi | DIP-59491N |
IntActi | Q9WVK4, 8 interactors |
MINTi | Q9WVK4 |
STRINGi | 10090.ENSMUSP00000025684 |
PTM databases
iPTMneti | Q9WVK4 |
PhosphoSitePlusi | Q9WVK4 |
SwissPalmi | Q9WVK4 |
Proteomic databases
EPDi | Q9WVK4 |
jPOSTi | Q9WVK4 |
MaxQBi | Q9WVK4 |
PaxDbi | Q9WVK4 |
PRIDEi | Q9WVK4 |
Protocols and materials databases
Antibodypediai | 44098, 164 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772 ENSMUST00000202020; ENSMUSP00000144036; ENSMUSG00000107039 |
GeneIDi | 13660 |
KEGGi | mmu:13660 |
UCSCi | uc008ghz.1, mouse |
Organism-specific databases
CTDi | 10938 |
MGIi | MGI:1341878, Ehd1 |
Phylogenomic databases
eggNOGi | KOG1954, Eukaryota |
GeneTreei | ENSGT00940000158249 |
HOGENOMi | CLU_017595_1_1_1 |
InParanoidi | Q9WVK4 |
OMAi | MAMVRQE |
OrthoDBi | 377342at2759 |
PhylomeDBi | Q9WVK4 |
TreeFami | TF314429 |
Enzyme and pathway databases
Reactomei | R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
BioGRID-ORCSi | 13660, 0 hits in 18 CRISPR screens |
ChiTaRSi | Ehd1, mouse |
PROi | PR:Q9WVK4 |
RNActi | Q9WVK4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024772, Expressed in ascending aorta and 303 other tissues |
ExpressionAtlasi | Q9WVK4, baseline and differential |
Genevisiblei | Q9WVK4, MM |
Family and domain databases
CDDi | cd00052, EH, 1 hit |
InterProi | View protein in InterPro IPR040990, DUF5600 IPR022812, Dynamin_SF IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR000261, EH_dom IPR029951, EHD1/EHD3 IPR031692, EHD_N IPR030381, G_DYNAMIN_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR11216:SF127, PTHR11216:SF127, 1 hit |
Pfami | View protein in Pfam PF18150, DUF5600, 1 hit PF00350, Dynamin_N, 1 hit PF12763, EF-hand_4, 1 hit PF16880, EHD_N, 1 hit |
SMARTi | View protein in SMART SM00027, EH, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS50031, EH, 1 hit PS51718, G_DYNAMIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EHD1_MOUSE | |
Accessioni | Q9WVK4Primary (citable) accession number: Q9WVK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families