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Protein

EH domain-containing protein 1

Gene

Ehd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes (By similarity). Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (PubMed:15930129, PubMed:20159556). Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity). Plays a role in myoblast fusion (PubMed:21177873). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (PubMed:24373286). Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis (By similarity).By similarity4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPBy similarity1
Binding sitei258ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi65 – 72ATPBy similarity8
Calcium bindingi489 – 500PROSITE-ProRule annotationBy similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCilium biogenesis/degradation, Protein transport, Transport
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 1Curated
Alternative name(s):
PAST homolog 1Curated
Short name:
mPAST1Imported
Gene namesi
Name:Ehd1Imported
Synonyms:Past1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1341878 Ehd1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65G → R: Abolishes interaction with PACSIN2. 1 Publication1
Mutagenesisi485W → A: Abolishes interaction with PACSIN2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001461101 – 534EH domain-containing protein 1Add BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei355PhosphoserineCombined sources1
Modified residuei456PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9WVK4
MaxQBiQ9WVK4
PaxDbiQ9WVK4
PRIDEiQ9WVK4

PTM databases

iPTMnetiQ9WVK4
PhosphoSitePlusiQ9WVK4
SwissPalmiQ9WVK4

Expressioni

Tissue specificityi

Highly expressed in testis. Also expressed in kidney, heart, intestine, and brain.1 Publication

Developmental stagei

Expression is already noted at day 9.5 in the limb buds and pharyngeal arches and at day 10.5 in sclerotomes, at various elements of the branchial apparatus (mandible and hyoid), and in the occipital region. At day 15.5 expression peaks in cartilage, preceding hypertrophy and ossification, and at day 17.5 there is no expression in the bones.1 Publication

Gene expression databases

BgeeiENSMUSG00000024772 Expressed in 285 organ(s), highest expression level in ascending aorta
CleanExiMM_EHD1
ExpressionAtlasiQ9WVK4 baseline and differential
GenevisibleiQ9WVK4 MM

Interactioni

Subunit structurei

Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4, ATP-binding is required for heterooligomerization (By similarity). Interacts (via EH domain) with MICALL1 (via NPF1 motif); the interaction is direct and recruits EHD1 to membranes (PubMed:23572513). Interacts with RAB35; the interaction is indirect through MICALL1 and recruits EHD1 to membranes (PubMed:23572513). Interacts (via EH domain) with PACSIN2 (via NPF motifs); regulates localization to tubular recycling endosome membranes (PubMed:15930129). Interacts with PACSIN1 (PubMed:15930129). Interacts with RAB8A (By similarity). Interacts with FER1L5 (via second C2 domain) (PubMed:21177873). Interacts with MYOF (PubMed:21177873). Interacts with ZFYVE20 (By similarity). Interacts (via EH domain) with RAB11FIP2 (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199408, 2 interactors
DIPiDIP-59491N
IntActiQ9WVK4, 8 interactors
MINTiQ9WVK4
STRINGi10090.ENSMUSP00000025684

Structurei

3D structure databases

ProteinModelPortaliQ9WVK4
SMRiQ9WVK4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 286Dynamin-type GPROSITE-ProRule annotationAdd BLAST232
Domaini444 – 532EHPROSITE-ProRule annotationAdd BLAST89
Domaini476 – 511EF-handPROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 72G1 motifPROSITE-ProRule annotation8
Regioni91 – 92G2 motifPROSITE-ProRule annotation2
Regioni153 – 156G3 motifPROSITE-ProRule annotation4
Regioni219 – 222G4 motifPROSITE-ProRule annotation4
Regioni243G5 motifPROSITE-ProRule annotation1

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili198 – 227Sequence analysisAdd BLAST30

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.1 Publication

Sequence similaritiesi

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1954 Eukaryota
ENOG410XYGB LUCA
GeneTreeiENSGT00760000118985
HOVERGENiHBG018183
InParanoidiQ9WVK4
KOiK12483
OMAiRFMCAQM
OrthoDBiEOG091G04KA
PhylomeDBiQ9WVK4
TreeFamiTF314429

Family and domain databases

CDDicd00052 EH, 1 hit
InterProiView protein in InterPro
IPR022812 Dynamin_SF
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000261 EH_dom
IPR031692 EHD_N
IPR030381 G_DYNAMIN_dom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00350 Dynamin_N, 1 hit
PF12763 EF-hand_4, 1 hit
PF16880 EHD_N, 1 hit
SMARTiView protein in SMART
SM00027 EH, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS50031 EH, 1 hit
PS51718 G_DYNAMIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WVK4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL
60 70 80 90 100
EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV
110 120 130 140 150
MHGPTEGVVP GNALVVDPRR PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS
160 170 180 190 200
IIDTPGILSG EKQRISRGYD FAAVLEWFAE RVDRIILLFD AHKLDISDEF
210 220 230 240 250
SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL GKIINTPEVV
260 270 280 290 300
RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR
310 320 330 340 350
ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISS
360 370 380 390 400
GDFPSLRKMQ ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE
410 420 430 440 450
ESLMPSQAVK GGAFDGTMNG PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE
460 470 480 490 500
IFYTLSPVNG KITGANAKKE MVKSKLPNTV LGKIWKLADV DKDGLLDDEE
510 520 530
FALANHLIKV KLEGHELPAD LPPHLIPPSK RRHE
Length:534
Mass (Da):60,603
Last modified:November 1, 1999 - v1
Checksum:i35502055BC6164F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099186 mRNA Translation: AAD45423.1
AF173156 mRNA Translation: AAF24223.1
AK012896 mRNA Translation: BAB28540.1
CCDSiCCDS29501.1
RefSeqiNP_034249.1, NM_010119.5
UniGeneiMm.30169

Genome annotation databases

EnsembliENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772
ENSMUST00000202020; ENSMUSP00000144036; ENSMUSG00000107039
GeneIDi13660
KEGGimmu:13660
UCSCiuc008ghz.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099186 mRNA Translation: AAD45423.1
AF173156 mRNA Translation: AAF24223.1
AK012896 mRNA Translation: BAB28540.1
CCDSiCCDS29501.1
RefSeqiNP_034249.1, NM_010119.5
UniGeneiMm.30169

3D structure databases

ProteinModelPortaliQ9WVK4
SMRiQ9WVK4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199408, 2 interactors
DIPiDIP-59491N
IntActiQ9WVK4, 8 interactors
MINTiQ9WVK4
STRINGi10090.ENSMUSP00000025684

PTM databases

iPTMnetiQ9WVK4
PhosphoSitePlusiQ9WVK4
SwissPalmiQ9WVK4

Proteomic databases

EPDiQ9WVK4
MaxQBiQ9WVK4
PaxDbiQ9WVK4
PRIDEiQ9WVK4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772
ENSMUST00000202020; ENSMUSP00000144036; ENSMUSG00000107039
GeneIDi13660
KEGGimmu:13660
UCSCiuc008ghz.1 mouse

Organism-specific databases

CTDi10938
MGIiMGI:1341878 Ehd1

Phylogenomic databases

eggNOGiKOG1954 Eukaryota
ENOG410XYGB LUCA
GeneTreeiENSGT00760000118985
HOVERGENiHBG018183
InParanoidiQ9WVK4
KOiK12483
OMAiRFMCAQM
OrthoDBiEOG091G04KA
PhylomeDBiQ9WVK4
TreeFamiTF314429

Enzyme and pathway databases

ReactomeiR-MMU-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

ChiTaRSiEhd1 mouse
PROiPR:Q9WVK4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024772 Expressed in 285 organ(s), highest expression level in ascending aorta
CleanExiMM_EHD1
ExpressionAtlasiQ9WVK4 baseline and differential
GenevisibleiQ9WVK4 MM

Family and domain databases

CDDicd00052 EH, 1 hit
InterProiView protein in InterPro
IPR022812 Dynamin_SF
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000261 EH_dom
IPR031692 EHD_N
IPR030381 G_DYNAMIN_dom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00350 Dynamin_N, 1 hit
PF12763 EF-hand_4, 1 hit
PF16880 EHD_N, 1 hit
SMARTiView protein in SMART
SM00027 EH, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS50031 EH, 1 hit
PS51718 G_DYNAMIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEHD1_MOUSE
AccessioniPrimary (citable) accession number: Q9WVK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1999
Last modified: September 12, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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