UniProtKB - Q9WVJ9 (FBLN4_MOUSE)
EGF-containing fibulin-like extracellular matrix protein 2
Efemp2
Functioni
Miscellaneous
GO - Molecular functioni
- calcium ion binding Source: InterPro
GO - Biological processi
- aorta development Source: UniProtKB
- aorta smooth muscle tissue morphogenesis Source: UniProtKB
- artery development Source: MGI
- elastic fiber assembly Source: UniProtKB
- negative regulation of vascular associated smooth muscle cell proliferation Source: UniProtKB
- positive regulation of aortic smooth muscle cell differentiation Source: UniProtKB
- positive regulation of collagen fibril organization Source: UniProtKB
- vascular associated smooth muscle cell development Source: UniProtKB
Keywordsi
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-MMU-2129379, Molecules associated with elastic fibres |
Names & Taxonomyi
Protein namesi | Recommended name: EGF-containing fibulin-like extracellular matrix protein 2CuratedAlternative name(s): Fibulin-41 Publication Short name: FIBL-4 Mutant p53-binding protein 1 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1891209, Efemp2 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix 3 Publications
- basement membrane 1 Publication
Note: Localizes on the microfibrils surrounding ELN cores.1 Publication
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
- elastic fiber Source: UniProtKB
- extracellular matrix Source: MGI
- extracellular space Source: BHF-UCL
Keywords - Cellular componenti
Basement membrane, Extracellular matrix, SecretedPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 57 | E → K: Knockin mutant mice are viable and survive into adulthood. Mice display abnormalities in multiple organ systems, including loose skin, bent forelimb, aortic aneurysm, tortuous artery, and pulmonary emphysema. In addition to elastic fiber abnormalities in the skin and large arteries, collagen fibrils are irregularly shaped, with many large fibrils in the dermis. Mice have large artery stiffness and systolic hypertension. Reduces protein secretion. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | 1 PublicationAdd BLAST | 27 | |
ChainiPRO_0000007576 | 28 – 443 | EGF-containing fibulin-like extracellular matrix protein 2Add BLAST | 416 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 28 | Pyrrolidone carboxylic acid1 Publication | 1 | |
Disulfide bondi | 58 ↔ 121 | PROSITE-ProRule annotation | ||
Disulfide bondi | 65 ↔ 80 | PROSITE-ProRule annotation | ||
Disulfide bondi | 71 ↔ 109 | PROSITE-ProRule annotation | ||
Disulfide bondi | 127 ↔ 140 | PROSITE-ProRule annotation | ||
Disulfide bondi | 134 ↔ 149 | PROSITE-ProRule annotation | ||
Disulfide bondi | 151 ↔ 162 | PROSITE-ProRule annotation | ||
Disulfide bondi | 168 ↔ 177 | PROSITE-ProRule annotation | ||
Disulfide bondi | 173 ↔ 186 | PROSITE-ProRule annotation | ||
Disulfide bondi | 188 ↔ 201 | PROSITE-ProRule annotation | ||
Glycosylationi | 198 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 207 ↔ 217 | PROSITE-ProRule annotation | ||
Disulfide bondi | 213 ↔ 226 | PROSITE-ProRule annotation | ||
Disulfide bondi | 228 ↔ 241 | PROSITE-ProRule annotation | ||
Disulfide bondi | 247 ↔ 258 | PROSITE-ProRule annotation | ||
Disulfide bondi | 254 ↔ 267 | PROSITE-ProRule annotation | ||
Disulfide bondi | 269 ↔ 281 | PROSITE-ProRule annotation | ||
Disulfide bondi | 287 ↔ 300 | PROSITE-ProRule annotation | ||
Disulfide bondi | 294 ↔ 309 | PROSITE-ProRule annotation | ||
Disulfide bondi | 315 ↔ 327 | PROSITE-ProRule annotation | ||
Glycosylationi | 394 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 87 – 88 | Cleavage; by ELANEBy similarity | 2 | |
Sitei | 90 – 91 | Cleavage; by MMP2, MMP3, MMP7, MMP9, MMP12By similarity | 2 | |
Sitei | 92 – 93 | CleavageBy similarity | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acidProteomic databases
MaxQBi | Q9WVJ9 |
PaxDbi | Q9WVJ9 |
PRIDEi | Q9WVJ9 |
PTM databases
GlyGeni | Q9WVJ9, 2 sites |
PhosphoSitePlusi | Q9WVJ9 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000024909, Expressed in humerus cartilage element and 252 other tissues |
ExpressionAtlasi | Q9WVJ9, baseline and differential |
Genevisiblei | Q9WVJ9, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (By similarity). Multimer; allows heparin binding (By similarity). Monomer (PubMed:17324935). Binds preferentially to p53 mutants (PubMed:10380882).
Interacts with FBN1 (via N-terminal domain); this interaction inhibits EFEMP2 binding to LOX and ELN (By similarity).
Interacts with ELN with moderate affinity; this interaction regulates ELN self-assembly maturation stage (PubMed:16478991, PubMed:17324935).
Interacts with PCOLCE (PubMed:26220971).
Interacts with collagen type IV trimer (COL4A1-COL4A1-COL4A2), NID2 and moderately with COL15A1-derived endostatin (PubMed:17324935).
Interacts with EMILIN1; this interaction promotes the incorporation of EFEMP2 into the extracellular matrix (PubMed:28717224).
Interacts with LTBP4; the LTBP4 long form (LTBP4L) has a stronger binding affinity than the LTBP4 short form and the LTBP4 long form promotes fibrillar deposition of EFEMP2 (PubMed:25713297).
Interacts with LOX (via propeptide); this interaction is strong and facilitates formation of ternary complexes with ELN during elastic fiber assembly; this interaction limits interation of EFEMP2 with FBLN5 (By similarity).
Interacts with PITX2 (By similarity).
Interacts with FBLN5 with moderate affinity (By similarity).
Interacts with LOXL1 (via propeptide), LTBP1 and TGFB1 stronger than with LOXL2 and LTBP3 (By similarity).
By similarity6 PublicationsProtein-protein interaction databases
BioGRIDi | 208447, 1 interactor |
IntActi | Q9WVJ9, 1 interactor |
STRINGi | 10090.ENSMUSP00000064719 |
Miscellaneous databases
RNActi | Q9WVJ9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 81 | EGF-like 1; atypicalPROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 123 – 163 | EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 41 | |
Domaini | 164 – 202 | EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 203 – 242 | EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 243 – 282 | EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 283 – 328 | EGF-like 6; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 46 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00940000159437 |
HOGENOMi | CLU_004826_0_1_1 |
InParanoidi | Q9WVJ9 |
OMAi | QQCHNLP |
PhylomeDBi | Q9WVJ9 |
Family and domain databases
InterProi | View protein in InterPro IPR026823, cEGF IPR026824, Efemp2 IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf |
PANTHERi | PTHR24034:SF96, PTHR24034:SF96, 1 hit |
Pfami | View protein in Pfam PF12662, cEGF, 2 hits PF07645, EGF_CA, 3 hits |
SMARTi | View protein in SMART SM00181, EGF, 5 hits SM00179, EGF_CA, 6 hits |
SUPFAMi | SSF57184, SSF57184, 3 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 4 hits PS01186, EGF_2, 4 hits PS50026, EGF_3, 4 hits PS01187, EGF_CA, 6 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 12 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MLPFASCLPG SLLLWAFLLL LLGAASPQDP EEPDSYTECT DGYEWDADSQ
60 70 80 90 100
HCRDVNECLT IPEACKGEMK CINHYGGYLC LPRSAAVISD LHGEGPPPPA
110 120 130 140 150
AHAQQPNPCP QGYEPDEQES CVDVDECTQA LHDCRPSQDC HNLPGSYQCT
160 170 180 190 200
CPDGYRKIGP ECVDIDECRY RYCQHRCVNL PGSFRCQCEP GFQLGPNNRS
210 220 230 240 250
CVDVNECDMG APCEQRCFNS YGTFLCRCNQ GYELHRDGFS CSDIDECGYS
260 270 280 290 300
SYLCQYRCVN EPGRFSCHCP QGYQLLATRL CQDIDECETG AHQCSEAQTC
310 320 330 340 350
VNFHGGYRCV DTNRCVEPYV QVSDNRCLCP ASNPLCREQP SSIVHRYMSI
360 370 380 390 400
TSERSVPADV FQIQATSVYP GAYNAFQIRS GNTQGDFYIR QINNVSAMLV
410 420 430 440
LARPVTGPRE YVLDLEMVTM NSLMSYRASS VLRLTVFVGA YTF
Computationally mapped potential isoform sequencesi
There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q3N9 | E9Q3N9_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 402 | Annotation score: | ||
A0A494BAR1 | A0A494BAR1_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 404 | Annotation score: | ||
A0A494BBF0 | A0A494BBF0_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 455 | Annotation score: | ||
E9Q3F3 | E9Q3F3_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 280 | Annotation score: | ||
E9Q2T8 | E9Q2T8_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 191 | Annotation score: | ||
G5E8D6 | G5E8D6_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 462 | Annotation score: | ||
E9Q1U0 | E9Q1U0_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 128 | Annotation score: | ||
E9Q2A0 | E9Q2A0_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 153 | Annotation score: | ||
E9PYW6 | E9PYW6_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 82 | Annotation score: | ||
F6Z1C2 | F6Z1C2_MOUSE | EGF-containing fibulin-like extrace... | Efemp2 | 157 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 | P → T AA sequence (PubMed:17324935).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104223 mRNA Translation: AAD45219.1 BC012269 mRNA Translation: AAH12269.1 |
CCDSi | CCDS29466.1 |
RefSeqi | NP_067449.3, NM_021474.3 XP_006531871.1, XM_006531808.1 |
Genome annotation databases
Ensembli | ENSMUST00000165485; ENSMUSP00000133016; ENSMUSG00000024909 ENSMUST00000166303; ENSMUSP00000128827; ENSMUSG00000024909 |
GeneIDi | 58859 |
KEGGi | mmu:58859 |
UCSCi | uc008gdl.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104223 mRNA Translation: AAD45219.1 BC012269 mRNA Translation: AAH12269.1 |
CCDSi | CCDS29466.1 |
RefSeqi | NP_067449.3, NM_021474.3 XP_006531871.1, XM_006531808.1 |
3D structure databases
SMRi | Q9WVJ9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208447, 1 interactor |
IntActi | Q9WVJ9, 1 interactor |
STRINGi | 10090.ENSMUSP00000064719 |
PTM databases
GlyGeni | Q9WVJ9, 2 sites |
PhosphoSitePlusi | Q9WVJ9 |
Proteomic databases
MaxQBi | Q9WVJ9 |
PaxDbi | Q9WVJ9 |
PRIDEi | Q9WVJ9 |
Protocols and materials databases
Antibodypediai | 16033, 359 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000165485; ENSMUSP00000133016; ENSMUSG00000024909 ENSMUST00000166303; ENSMUSP00000128827; ENSMUSG00000024909 |
GeneIDi | 58859 |
KEGGi | mmu:58859 |
UCSCi | uc008gdl.2, mouse |
Organism-specific databases
CTDi | 30008 |
MGIi | MGI:1891209, Efemp2 |
Phylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00940000159437 |
HOGENOMi | CLU_004826_0_1_1 |
InParanoidi | Q9WVJ9 |
OMAi | QQCHNLP |
PhylomeDBi | Q9WVJ9 |
Enzyme and pathway databases
Reactomei | R-MMU-2129379, Molecules associated with elastic fibres |
Miscellaneous databases
BioGRID-ORCSi | 58859, 5 hits in 17 CRISPR screens |
ChiTaRSi | Efemp2, mouse |
PROi | PR:Q9WVJ9 |
RNActi | Q9WVJ9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024909, Expressed in humerus cartilage element and 252 other tissues |
ExpressionAtlasi | Q9WVJ9, baseline and differential |
Genevisiblei | Q9WVJ9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR026823, cEGF IPR026824, Efemp2 IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf |
PANTHERi | PTHR24034:SF96, PTHR24034:SF96, 1 hit |
Pfami | View protein in Pfam PF12662, cEGF, 2 hits PF07645, EGF_CA, 3 hits |
SMARTi | View protein in SMART SM00181, EGF, 5 hits SM00179, EGF_CA, 6 hits |
SUPFAMi | SSF57184, SSF57184, 3 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 4 hits PS01186, EGF_2, 4 hits PS50026, EGF_3, 4 hits PS01187, EGF_CA, 6 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FBLN4_MOUSE | |
Accessioni | Q9WVJ9Primary (citable) accession number: Q9WVJ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families