UniProtKB - Q9WVI9 (JIP1_MOUSE)
C-Jun-amino-terminal kinase-interacting protein 1
Mapk8ip1
Functioni
GO - Molecular functioni
- identical protein binding Source: MGI
- JUN kinase binding Source: MGI
- kinesin binding Source: UniProtKB
- MAP-kinase scaffold activity Source: MGI
- mitogen-activated protein kinase kinase binding Source: MGI
- mitogen-activated protein kinase kinase kinase binding Source: MGI
- protein kinase binding Source: UniProtKB
GO - Biological processi
- JUN phosphorylation Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of intrinsic apoptotic signaling pathway Source: MGI
- negative regulation of JNK cascade Source: MGI
- negative regulation of JUN kinase activity Source: UniProtKB
- positive regulation of JNK cascade Source: UniProtKB
- regulation of CD8-positive, alpha-beta T cell proliferation Source: UniProtKB
- regulation of JNK cascade Source: UniProtKB
- regulation of transcription, DNA-templated Source: MGI
- signal transduction Source: MGI
- vesicle-mediated transport Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: C-Jun-amino-terminal kinase-interacting protein 1Short name: JIP-1 Short name: JNK-interacting protein 1 Alternative name(s): Islet-brain-1 Short name: IB-1 JNK MAP kinase scaffold protein 1 Mitogen-activated protein kinase 8-interacting protein 1 |
Gene namesi | Name:Mapk8ip1 Synonyms:Ib1, Jip1, Mapk8ip, Prkm8ip |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1309464, Mapk8ip1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity
Nucleus
- Nucleus By similarity
Mitochondrion
- Mitochondrion membrane By similarity
Other locations
- Cytoplasm By similarity
- perinuclear region By similarity
Note: Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- axon Source: MGI
- axonal growth cone Source: MGI
- cell body Source: MGI
- cytoplasm Source: UniProtKB
- dendrite Source: MGI
- dendritic growth cone Source: MGI
- dentate gyrus mossy fiber Source: MGI
- membrane Source: UniProtKB
- neuron projection Source: MGI
- perinuclear region of cytoplasm Source: MGI
- synapse Source: MGI
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000220629 | 1 – 707 | C-Jun-amino-terminal kinase-interacting protein 1Add BLAST | 707 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineCombined sources | 1 | |
Modified residuei | 28 | PhosphoserineCombined sources | 1 | |
Modified residuei | 39 | PhosphoserineBy similarity | 1 | |
Modified residuei | 103 | Phosphothreonine; by MAPK8, MAPK9 and MAPK10By similarity | 1 | |
Modified residuei | 148 | PhosphoserineBy similarity | 1 | |
Modified residuei | 177 | PhosphoserineBy similarity | 1 | |
Modified residuei | 183 | PhosphoserineBy similarity | 1 | |
Modified residuei | 189 | PhosphoserineBy similarity | 1 | |
Modified residuei | 191 | PhosphoserineBy similarity | 1 | |
Modified residuei | 192 | PhosphoserineBy similarity | 1 | |
Modified residuei | 201 | Phosphothreonine; by MAPK8, MAPK9 and MAPK10By similarity | 1 | |
Modified residuei | 210 | PhosphoserineBy similarity | 1 | |
Modified residuei | 307 | PhosphoserineBy similarity | 1 | |
Modified residuei | 324 | PhosphoserineBy similarity | 1 | |
Modified residuei | 326 | PhosphoserineBy similarity | 1 | |
Modified residuei | 336 | PhosphoserineBy similarity | 1 | |
Modified residuei | 351 | PhosphoserineBy similarity | 1 | |
Modified residuei | 362 | PhosphoserineBy similarity | 1 | |
Modified residuei | 365 | PhosphoserineBy similarity | 1 | |
Modified residuei | 403 | PhosphoserineBy similarity | 1 | |
Modified residuei | 405 | PhosphoserineBy similarity | 1 | |
Modified residuei | 407 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 440 | PhosphoserineBy similarity | 1 | |
Modified residuei | 443 | PhosphoserineBy similarity | 1 | |
Modified residuei | 444 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 465 | PhosphoserineBy similarity | 1 | |
Modified residuei | 467 | PhosphoserineBy similarity | 1 | |
Modified residuei | 468 | PhosphoserineBy similarity | 1 | |
Modified residuei | 469 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q9WVI9 |
PeptideAtlasi | Q9WVI9 |
PRIDEi | Q9WVI9 |
PTM databases
iPTMneti | Q9WVI9 |
PhosphoSitePlusi | Q9WVI9 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000027223, Expressed in cingulate cortex and 275 other tissues |
Genevisiblei | Q9WVI9, MM |
Interactioni
Subunit structurei
Forms homo- or heterooligomeric complexes. Binds specific components of the JNK signaling pathway namely MAPK8/JNK1, MAPK9/JNK2, MAPK10/JNK3, MAP2K7/MKK7, MAP3K11/MLK3 and DLK1. Also binds the proline-rich domain-containing splice variant of apolipoprotein E receptor 2 (ApoER2) (By similarity). Binds the cytoplasmic tails of LRP1 and LRP2 (Megalin). Binds the TPR motif-containing C-terminal of kinesin light chain, KLC1. Pre-assembled MAPK8IP1 scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location.
Interacts with the cytoplasmic domain of APP (By similarity).
Interacts, via the PID domain, with ARHGEF28.
Interacts with MAP3K7/TAK1 and VRK2 (By similarity).
Interacts with DCLK2 (PubMed:16628014).
Found in a complex with SH3RF1, RAC1, MAP3K11/MLK3, MAP2K7/MKK7 and MAPK8/JNK1 (PubMed:23963642).
Found in a complex with SH3RF1, RAC2, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and MAPK9/JNK2 (PubMed:27084103).
Interacts with SH3RF2 (By similarity).
By similarity6 PublicationsBinary interactionsi
Hide detailsQ9WVI9
With | #Exp. | IntAct |
---|---|---|
Lrp1 [Q91ZX7] | 2 | EBI-74515,EBI-300955 |
Lrp2 [A2ARV4] | 2 | EBI-74515,EBI-300875 |
Isoform JIP-1b [Q9WVI9-1]
With | #Exp. | IntAct |
---|---|---|
App [P12023] | 3 | EBI-288461,EBI-78814 |
App - isoform APP695 [P12023-2] | 2 | EBI-288461,EBI-286828 |
Lrp1b [Q9JI18] | 3 | EBI-288461,EBI-8294317 |
MAPK8 [P45983] from Homo sapiens. | 2 | EBI-288461,EBI-286483 |
Isoform JIP-1a [Q9WVI9-2]
With | #Exp. | IntAct |
---|---|---|
Map3k7 [Q62073] | 4 | EBI-288464,EBI-1775345 |
VRK2 - isoform 2 [Q86Y07-2] from Homo sapiens. | 2 | EBI-288464,EBI-1207636 |
GO - Molecular functioni
- identical protein binding Source: MGI
- JUN kinase binding Source: MGI
- kinesin binding Source: UniProtKB
- MAP-kinase scaffold activity Source: MGI
- mitogen-activated protein kinase kinase binding Source: MGI
- mitogen-activated protein kinase kinase kinase binding Source: MGI
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 202375, 9 interactors |
IntActi | Q9WVI9, 18 interactors |
MINTi | Q9WVI9 |
STRINGi | 10090.ENSMUSP00000050773 |
Miscellaneous databases
RNActi | Q9WVI9, protein |
Structurei
3D structure databases
SMRi | Q9WVI9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9WVI9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 484 – 545 | SH3PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 557 – 696 | PIDPROSITE-ProRule annotationAdd BLAST | 140 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 127 – 281 | JNK-binding domain (JBD)Add BLAST | 155 | |
Regioni | 153 – 172 | Minimal inhibitory domain (MID)Add BLAST | 20 | |
Regioni | 279 – 467 | Interaction with MAP3K7By similarityAdd BLAST | 189 | |
Regioni | 467 – 656 | Interaction with VRK2By similarityAdd BLAST | 190 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 349 – 356 | D-box 1 | 8 | |
Motifi | 360 – 368 | D-box 2 | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 41 – 47 | Asp/Glu-rich (acidic) | 7 | |
Compositional biasi | 107 – 116 | Asp/Glu-rich (acidic) | 10 | |
Compositional biasi | 355 – 359 | Poly-Pro | 5 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG3775, Eukaryota |
GeneTreei | ENSGT00940000157089 |
HOGENOMi | CLU_006711_1_1_1 |
InParanoidi | Q9WVI9 |
OMAi | QGNKCSH |
OrthoDBi | 372907at2759 |
PhylomeDBi | Q9WVI9 |
TreeFami | TF325073 |
Family and domain databases
CDDi | cd11943, SH3_JIP1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
IDEALi | IID50056 |
InterProi | View protein in InterPro IPR035638, JIP1_SH3 IPR011993, PH-like_dom_sf IPR006020, PTB/PI_dom IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00640, PID, 1 hit PF14604, SH3_9, 1 hit |
SMARTi | View protein in SMART SM00462, PTB, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS01179, PID, 1 hit PS50002, SH3, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAERESGLGG GAASPPAASP FLGLHIASPP NFRLTHDISL EEFEDEDLSE
60 70 80 90 100
ITDECGISLQ CKDTLSLRPP RAGLLSAGSS GSAGSRLQAE MLQMDLIDAA
110 120 130 140 150
GDTPGAEDDE EEEDDELAAQ RPGVGPPKAE SNQDPAPRSQ GQGPGTGSGD
160 170 180 190 200
TYRPKRPTTL NLFPQVPRSQ DTLNNNSLGK KHSWQDRVSR SSSPLKTGEQ
210 220 230 240 250
TPPHEHICLS DELPPQGSPV PTQDRGTSTD SPCRRSAATQ MAPPSGPPAT
260 270 280 290 300
APGGRGHSHR DRIHYQADVR LEATEEIYLT PVQRPPDPAE PTSTFMPPTE
310 320 330 340 350
SRMSVSSDPD PAAYSVTAGR PHPSISEEDE GFDCLSSPER AEPPGGGWRG
360 370 380 390 400
SLGEPPPPPR ASLSSDTSAL SYDSVKYTLV VDEHAQLELV SLRPCFGDYS
410 420 430 440 450
DESDSATVYD NCASASSPYE SAIGEEYEEA PQPRPPTCLS EDSTPDEPDV
460 470 480 490 500
HFSKKFLNVF MSGRSRSSSA ESFGLFSCVI NGEEHEQTHR AIFRFVPRHE
510 520 530 540 550
DELELEVDDP LLVELQAEDY WYEAYNMRTG ARGVFPAYYA IEVTKEPEHM
560 570 580 590 600
AALAKNSDWI DQFRVKFLGS VQVPYHKGND VLCAAMQKIA TTRRLTVHFN
610 620 630 640 650
PPSSCVLEIS VRGVKIGVKA DDALEAKGNK CSHFFQLKNI SFCGYHPKNN
660 670 680 690 700
KYFGFITKHP ADHRFACHVF VSEDSTKALA ESVGRAFQQF YKQFVEYTCP
TEDIYLE
The sequence of this isoform differs from the canonical sequence as follows:
558-604: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-33: MAERESGLGGGAASPPAASPFLGLHIASPPNFR → MQLVLKMDSSPDNDSWLEDQWEHW
The sequence of this isoform differs from the canonical sequence as follows:
1-33: MAERESGLGGGAASPPAASPFLGLHIASPPNFR → MQLVLKMDSSPDNDSWLEDQWEHW
69-93: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-90: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 144 – 145 | PG → A in AAD38346 (PubMed:10098834).Curated | 2 | |
Sequence conflicti | 144 – 145 | PG → A in AAD38347 (PubMed:10098834).Curated | 2 | |
Sequence conflicti | 144 – 145 | PG → A in AAD38348 (PubMed:10098834).Curated | 2 | |
Sequence conflicti | 593 | R → RP in AAD38346 (PubMed:10098834).Curated | 1 | |
Sequence conflicti | 593 | R → RP in AAD38347 (PubMed:10098834).Curated | 1 | |
Sequence conflicti | 593 | R → RP in AAD38348 (PubMed:10098834).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 10 | G → R in strain: ILS. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002764 | 1 – 90 | Missing in isoform JIP-1e. 1 PublicationAdd BLAST | 90 | |
Alternative sequenceiVSP_002763 | 1 – 33 | MAERE…PPNFR → MQLVLKMDSSPDNDSWLEDQ WEHW in isoform JIP-1c and isoform JIP-1d. 1 PublicationAdd BLAST | 33 | |
Alternative sequenceiVSP_002765 | 69 – 93 | Missing in isoform JIP-1d. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_002766 | 558 – 604 | Missing in isoform JIP-1a. 1 PublicationAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF003115 mRNA Translation: AAB66317.1 AF109768 mRNA Translation: AAD38346.1 AF109769 mRNA Translation: AAD38347.1 AF109770 mRNA Translation: AAD38348.1 AF109771 mRNA Translation: AAD38349.1 AF054611 mRNA Translation: AAD22580.1 AF332075 mRNA Translation: AAK56103.1 AF332076 mRNA Translation: AAK56104.1 |
CCDSi | CCDS16446.1 [Q9WVI9-1] CCDS57180.1 [Q9WVI9-3] |
PIRi | T03038 |
RefSeqi | NP_035292.2, NM_011162.5 [Q9WVI9-1] |
Genome annotation databases
Ensembli | ENSMUST00000050312; ENSMUSP00000050773; ENSMUSG00000027223 [Q9WVI9-1] ENSMUST00000111279; ENSMUSP00000106910; ENSMUSG00000027223 [Q9WVI9-3] |
GeneIDi | 19099 |
KEGGi | mmu:19099 |
UCSCi | uc008kxv.2, mouse [Q9WVI9-3] uc008kxw.2, mouse [Q9WVI9-4] uc008kxx.2, mouse [Q9WVI9-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF003115 mRNA Translation: AAB66317.1 AF109768 mRNA Translation: AAD38346.1 AF109769 mRNA Translation: AAD38347.1 AF109770 mRNA Translation: AAD38348.1 AF109771 mRNA Translation: AAD38349.1 AF054611 mRNA Translation: AAD22580.1 AF332075 mRNA Translation: AAK56103.1 AF332076 mRNA Translation: AAK56104.1 |
CCDSi | CCDS16446.1 [Q9WVI9-1] CCDS57180.1 [Q9WVI9-3] |
PIRi | T03038 |
RefSeqi | NP_035292.2, NM_011162.5 [Q9WVI9-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UKH | X-ray | 2.35 | B | 153-163 | [»] | |
1UKI | X-ray | 2.70 | B | 153-163 | [»] | |
3O17 | X-ray | 3.00 | F/G | 154-163 | [»] | |
3O2M | X-ray | 2.70 | F/G | 154-163 | [»] | |
3V3V | X-ray | 2.70 | B | 153-163 | [»] | |
6F5E | X-ray | 2.70 | C | 153-163 | [»] | |
SMRi | Q9WVI9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202375, 9 interactors |
IntActi | Q9WVI9, 18 interactors |
MINTi | Q9WVI9 |
STRINGi | 10090.ENSMUSP00000050773 |
PTM databases
iPTMneti | Q9WVI9 |
PhosphoSitePlusi | Q9WVI9 |
Proteomic databases
PaxDbi | Q9WVI9 |
PeptideAtlasi | Q9WVI9 |
PRIDEi | Q9WVI9 |
Protocols and materials databases
Antibodypediai | 26197, 261 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000050312; ENSMUSP00000050773; ENSMUSG00000027223 [Q9WVI9-1] ENSMUST00000111279; ENSMUSP00000106910; ENSMUSG00000027223 [Q9WVI9-3] |
GeneIDi | 19099 |
KEGGi | mmu:19099 |
UCSCi | uc008kxv.2, mouse [Q9WVI9-3] uc008kxw.2, mouse [Q9WVI9-4] uc008kxx.2, mouse [Q9WVI9-1] |
Organism-specific databases
CTDi | 9479 |
MGIi | MGI:1309464, Mapk8ip1 |
Phylogenomic databases
eggNOGi | KOG3775, Eukaryota |
GeneTreei | ENSGT00940000157089 |
HOGENOMi | CLU_006711_1_1_1 |
InParanoidi | Q9WVI9 |
OMAi | QGNKCSH |
OrthoDBi | 372907at2759 |
PhylomeDBi | Q9WVI9 |
TreeFami | TF325073 |
Miscellaneous databases
BioGRID-ORCSi | 19099, 0 hits in 17 CRISPR screens |
EvolutionaryTracei | Q9WVI9 |
PROi | PR:Q9WVI9 |
RNActi | Q9WVI9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027223, Expressed in cingulate cortex and 275 other tissues |
Genevisiblei | Q9WVI9, MM |
Family and domain databases
CDDi | cd11943, SH3_JIP1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
IDEALi | IID50056 |
InterProi | View protein in InterPro IPR035638, JIP1_SH3 IPR011993, PH-like_dom_sf IPR006020, PTB/PI_dom IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00640, PID, 1 hit PF14604, SH3_9, 1 hit |
SMARTi | View protein in SMART SM00462, PTB, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS01179, PID, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | JIP1_MOUSE | |
Accessioni | Q9WVI9Primary (citable) accession number: Q9WVI9 Secondary accession number(s): O35145 Q9WVI8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 5, 2001 |
Last sequence update: | December 5, 2001 | |
Last modified: | December 2, 2020 | |
This is version 195 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families