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UniProtKB - Q9WVE9 (ITSN1_RAT)

Entry version 165 (10 Feb 2021)
Sequence version 2 (05 Dec 2018)
Previous versions | rss
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Protein

Intersectin-1

Gene

Itsn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery. Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (By similarity). Plays a role in the assembly and maturation of clathrin-coated vesicles (PubMed:20448150). Recruits FCHSD2 to clathrin-coated pits (By similarity). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (By similarity). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (By similarity). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines (By similarity). Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (PubMed:23633571). Inhibits ARHGAP31 activity toward RAC1 (By similarity).By similarity2 Publications
Plays a role in synaptic vesicle endocytosis in brain neurons.By similarity

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Ca2+PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi1659CalciumPROSITE-ProRule annotation1
Metal bindingi1662CalciumPROSITE-ProRule annotation1
Metal bindingi1665CalciumPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) of the calcium-binding region(s) within the protein. One common calcium-binding motif is the EF-hand, but other calcium-binding motifs also exist.<p><a href='/help/ca_bind' target='_top'>More...</a></p>Calcium bindingi66 – 771PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi267 – 2782PROSITE-ProRule annotationAdd BLAST12

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processEndocytosis, Exocytosis, Protein transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-RNO-193648, NRAGE signals death through JNK
R-RNO-194840, Rho GTPase cycle
R-RNO-3928662, EPHB-mediated forward signaling
R-RNO-416482, G alpha (12/13) signalling events
R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828, Clathrin-mediated endocytosis

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Intersectin-1
Alternative name(s):
EH domain and SH3 domain regulator of endocytosis 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Itsn1
Synonyms:Ehsh11 Publication, Itsn
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRattus norvegicus (Rat)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10116 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002494 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 11
  • UP000234681 Componentsi: Chromosome 11, Unassembled WGS sequence

Organism-specific databases

Rat genome database

More...RGDi		2935, Itsn1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Coated pit, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane, Nucleus, Synapse, Synaptosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000809591 – 1713Intersectin-1Add BLAST1713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei203PhosphoserineCombined sources1
Modified residuei318PhosphoserineBy similarity1
Modified residuei334PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei685PhosphoserineCombined sources1
Modified residuei889PhosphothreonineBy similarity1
Modified residuei893PhosphoserineBy similarity1
Modified residuei894PhosphoserineCombined sources1
Modified residuei896PhosphoserineCombined sources1
Modified residuei970PhosphoserineCombined sources1
Modified residuei976PhosphothreonineBy similarity1
Modified residuei987PhosphoserineBy similarity1
Modified residuei1129PhosphoserineCombined sources1
Modified residuei1136PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q9WVE9

PRoteomics IDEntifications database

More...PRIDEi		Q9WVE9

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q9WVE9

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q9WVE9

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Detected in brain, spleen, lung, liver and heart (at protein level). Ubiquitous. Detected in brain, spleen, lung, liver, skeletal muscle and kidney.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSRNOG00000002001, Expressed in skeletal muscle tissue and 20 other tissues

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts (via DH domain) with CDC42.

Interacts (via SH3 domain 1) with WASL (By similarity).

Interacts with dynamin, SNAP25 and SNAP23 (PubMed:10373452).

Interacts with clathrin-associated proteins and other components of the endocytic machinery, such as SPIN90, EPS15, EPN1, EPN2, STON2, FCHO1, FCHO2 and DAB2 (PubMed:20448150).

Interacts (via SH3 domains) with REPS1 and SGIP1.

Interacts with ARHGAP31.

Interacts with ADAM15 (By similarity).

Interacts with PRRT2 (PubMed:26797119).

Interacts (via SH3 domain 4) with FCHSD2 (via SH3 domain 2).

Interacts (via SH3 domain 1) with DENND2B (By similarity).

Interacts (via SH3 domains) with CBL (By similarity). Isoform 2:

Interacts with CBL and DNM1. Isoform 2:

Interacts with LMNA. Isoform 2:

Interacts with importin subunit KPNA1; this is likely to mediate its import into the nucleus (By similarity).

By similarity3 Publications

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		248132, 5 interactors

Protein interaction database and analysis system

More...IntActi		Q9WVE9, 3 interactors

Molecular INTeraction database

More...MINTi		Q9WVE9

STRING: functional protein association networks

More...STRINGi		10116.ENSRNOP00000045513

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q9WVE9

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q9WVE9

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini21 – 109EH 1PROSITE-ProRule annotationAdd BLAST89
Domaini53 – 88EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini221 – 310EH 2PROSITE-ProRule annotationAdd BLAST90
Domaini254 – 289EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini737 – 798SH3 1PROSITE-ProRule annotationAdd BLAST62
Domaini905 – 963SH3 2PROSITE-ProRule annotationAdd BLAST59
Domaini994 – 1052SH3 3PROSITE-ProRule annotationAdd BLAST59
Domaini1066 – 1130SH3 4PROSITE-ProRule annotationAdd BLAST65
Domaini1147 – 1206SH3 5PROSITE-ProRule annotationAdd BLAST60
Domaini1229 – 1415DHPROSITE-ProRule annotationAdd BLAST187
Domaini1454 – 1563PHPROSITE-ProRule annotationAdd BLAST110
Domaini1571 – 1687C2PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni326 – 702KLERQAdd BLAST377
Regioni1066 – 1130Required for interaction with FCHSD2By similarityAdd BLAST65

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili351 – 670Sequence analysisAdd BLAST320

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi1096 – 1119Bipartite nuclear localization signal; in isoform 2By similarityAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi331 – 449Glu-richPROSITE-ProRule annotationAdd BLAST119
Compositional biasi367 – 453Arg-richPROSITE-ProRule annotationAdd BLAST87
Compositional biasi527 – 669Gln-richPROSITE-ProRule annotationAdd BLAST143

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

SH3-1 and SH3-4 bind to ARHGAP31 (By similarity). SH3-3, SH3-4 and SH3-5, but not SH3-1 and SH3-2 domains, bind to dynamin.By similarity
The KLERQ domain binds to SNAP-25 and SNAP-23.
In an autoinhibited form the SH3 domain 5 may bind intramolecularly to the DH domain, thus blocking the CDC42-binding site.By similarity

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1029, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000157065

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_002819_2_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q9WVE9

Identification of Orthologs from Complete Genome Data

More...OMAi		RAVYEFN

Database of Orthologous Groups

More...OrthoDBi		807060at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q9WVE9

Family and domain databases

Conserved Domains Database

More...CDDi		cd00052, EH, 2 hits
cd00160, RhoGEF, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.900.10, 1 hit
2.30.29.30, 1 hit
2.60.40.150, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR000008, C2_dom
IPR035892, C2_domain_sf
IPR035899, DBL_dom_sf
IPR000219, DH-domain
IPR011992, EF-hand-dom_pair
IPR018247, EF_Hand_1_Ca_BS
IPR002048, EF_hand_dom
IPR000261, EH_dom
IPR001331, GDS_CDC24_CS
IPR011993, PH-like_dom_sf
IPR001849, PH_domain
IPR036028, SH3-like_dom_sf
IPR001452, SH3_domain

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00168, C2, 1 hit
PF12763, EF-hand_4, 2 hits
PF16652, PH_13, 1 hit
PF00621, RhoGEF, 1 hit
PF00018, SH3_1, 2 hits
PF07653, SH3_2, 2 hits
PF14604, SH3_9, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00452, SH3DOMAIN

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00239, C2, 1 hit
SM00054, EFh, 2 hits
SM00027, EH, 2 hits
SM00233, PH, 1 hit
SM00325, RhoGEF, 1 hit
SM00326, SH3, 5 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF47473, SSF47473, 2 hits
SSF48065, SSF48065, 1 hit
SSF50044, SSF50044, 5 hits

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50004, C2, 1 hit
PS00741, DH_1, 1 hit
PS50010, DH_2, 1 hit
PS00018, EF_HAND_1, 2 hits
PS50222, EF_HAND_2, 2 hits
PS50031, EH, 2 hits
PS50003, PH_DOMAIN, 1 hit
PS50002, SH3, 5 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9WVE9-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQFPTPFGG SLDIWAITVE ERAKHDQQFQ SLKPISGFIT GDQARNFFFQ 
        60         70         80         90        100
SGLPQPVLAQ IWALADMNKD GRMDQVEFSI AMKLIKLKLQ GYQLPPALPP 
       110        120        130        140        150
VMKQQPAAIS SAPAFGIGGM AGMPPLTAVA PVPMGSIPVV GMSPPLVSSV 
       160        170        180        190        200
PQAAVPPLAN GAPPVIQPLP AFAHPAATLP KSSSFSRSGP GSQLNTKLQK 
       210        220        230        240        250
AQSFDVASAP AAAEWAVPQS SRLKYRQLFN SHDKTMSGHL TGPQARTILM 
       260        270        280        290        300
QSSLPQAQLA SIWNLSDIDQ DGKLTAEEFI LAMHLIDVAM SGQPLPPVLP 
       310        320        330        340        350
PEYIPPSFRR VRSGSGMSVI SSSSADQRLP EEPSSEDEQQ VEKKLPVTFE 
       360        370        380        390        400
DKKRENFERG NLELEKRRQA LLEQQRKEQE RLAQLERAEQ ERKERERQEQ 
       410        420        430        440        450
ERKRQLELEK QLEKQRELER QREEERRKEI ERREAAKREL ERQRQLEWER 
       460        470        480        490        500
NRRQELLTQR NKDQEGIVVL KARRKTLEFE LEALNDKKHQ LEGKLQDIRC 
       510        520        530        540        550
RLATQRQEIE STNKSRELRI AEITHLQQQL QESQQMLGRL IPEKQILSDQ 
       560        570        580        590        600
LKQVQQNSLH RDSLLTLKRA LEAKELARQQ LREQLDEVEK ETRSKLQEID 
       610        620        630        640        650
VFNNQLKELR EIHSKQQLQK QRSIEAERLK QKEQERKSLE LEKQKEEGQR 
       660        670        680        690        700
RVQERDKQWQ EHVQQEEQQR PRKPHEEDKL KREDSVKKKE AEERAKPEVQ 
       710        720        730        740        750
DKQSRLFHPH QEPAKPAQAP WPTTEKGPLT ISAQESAKVV YYRALYPFES 
       760        770        780        790        800
RSHDEITIQP GDIVMVDESQ TGEPGWLGGE LKGKTGWFPA NYAEKIPENE 
       810        820        830        840        850
IPTPAKPVTD LTSAPAPKLA LRETPAPLPV TSSEPSTTPN NWADFSSTWP 
       860        870        880        890        900
SSTNEKPETD NWDTWAAQPS LTVPSAGQLR QRSAFTPATA TGSSPSPVLG 
       910        920        930        940        950
QGEKVEGLQA QALYPWRAKK DNHLNFNKSD VITVLEQQDM WWFGEVQGQK 
       960        970        980        990       1000
GWFPKSYVKL ISGPVRKSTS IDTGPTEAPS SLKRVASPAA KPAIPGEEFV 
      1010       1020       1030       1040       1050
AMYTYESSEH GDLTFQQGDV IVVTKKDGDW WTGTVGETSG VFPSNYVRLK 
      1060       1070       1080       1090       1100
DSEGSGTAGK TGSLGKKPEI AQVIASYTAT GPEQLTLAPG QLILIRKKNP 
      1110       1120       1130       1140       1150
GGWWEGELQA RGKKRQIGWF PANYVKLLSP GTSKITPTEL PKTAVQPAVC 
      1160       1170       1180       1190       1200
QVIGMYDYTA QNDDELAFSK GQIINVLSKE DPDWWKGEVS GQVGLFPSNY 
      1210       1220       1230       1240       1250
VKLTTDMDPS QQWCSDLHLL DMLTPTERKR QGYIHELIVT EENYVNDLQL 
      1260       1270       1280       1290       1300
VTEIFQKPLT ESELLTEKEV AMIFVNWKEL IMCNIKLLKA LRVRKKMSGE 
      1310       1320       1330       1340       1350
KMPVKMIGDI LSAQLPHMQP YIRFCSCQLN GAALIQQKTD EAPDFKEFVK 
      1360       1370       1380       1390       1400
RLAMDPRCKG MPLSSFILKP MQRVTRYPLI IKNILENTPE NHPDHSHLKH 
      1410       1420       1430       1440       1450
ALEKAEELCS QVNEGVREKE NSDRLEWIQA HVQCEGLSEQ LVFNSVTNCL 
      1460       1470       1480       1490       1500
GPRKFLHSGK LYKAKSNKEL YGFLFNDFLL LTQITKPLGS SSTDKVFSPK 
      1510       1520       1530       1540       1550
SNLQYKMYKT PIFLNEVLVK LPTDPSGDEP IFHISHIDRV YTLRAESINE 
      1560       1570       1580       1590       1600
RTAWVQKIKA ASELYIETEK KKREKAYLVR SQRATGIGRL MVNVVEGIEL 
      1610       1620       1630       1640       1650
KPCRSHGKSN PYCEVTMGSQ CHITKTIQDT LNPKWNSNCQ FFIRDLEQEV 
      1660       1670       1680       1690       1700
LCITVFERDQ FSPDDFLGRT EIRVADIKKD QGSKGPVTKC LLLHEVPTGE 
      1710 
IVVRLDLQLF DEP
Length:1,713
Mass (Da):194,197
Last modified:December 5, 2018 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iDF5ED07492F716BA
GO
Isoform 2 (identifier: Q9WVE9-1) [UniParc]FASTAAdd to basket
Also known as: ITSN-sBy similarity

The sequence of this isoform differs from the canonical sequence as follows:
     766-766: V → VKGEWV
     1213-1713: Missing.

Show »
Length:1,217
Mass (Da):137,077
Checksum:iAF2B0693354C3BB8
GO
Isoform 3 (identifier: Q9WVE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     766-766: V → VKGEWV
     998-1068: Missing.
     1213-1713: Missing.

Show »
Length:1,146
Mass (Da):129,460
Checksum:i0E4CFDC5ACFF0C1D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti781L → P in AAD30271 (PubMed:10373452).Curated1
Sequence conflicti781L → P in AAD31026 (PubMed:10373452).Curated1
Sequence conflicti1019D → H in AAD30271 (PubMed:10373452).Curated1
Sequence conflicti1065G → E in AAD30271 (PubMed:10373452).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_059959766V → VKGEWV in isoform 2 and isoform 3. Curated1
Alternative sequenceiVSP_059960998 – 1068Missing in isoform 3. CuratedAdd BLAST71
Alternative sequenceiVSP_0599611213 – 1713Missing in isoform 2 and isoform 3. CuratedAdd BLAST501

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF127798 mRNA Translation: AAD30271.1
AF132672 mRNA Translation: AAD31026.1
AC123507 Genomic DNA No translation available.
AC142009 Genomic DNA No translation available.
CH473989 Genomic DNA Translation: EDM10741.1

NCBI Reference Sequences

More...RefSeqi		NP_001129568.1, NM_001136096.1 [Q9WVE9-3]
NP_062100.1, NM_019227.1

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSRNOT00000042750; ENSRNOP00000044773; ENSRNOG00000002001 [Q9WVE9-3]
ENSRNOT00000047843; ENSRNOP00000045513; ENSRNOG00000002001 [Q9WVE9-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		29491

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		rno:29491

UCSC genome browser

More...UCSCi		RGD:2935, rat [Q9WVE9-3]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127798 mRNA Translation: AAD30271.1
AF132672 mRNA Translation: AAD31026.1
AC123507 Genomic DNA No translation available.
AC142009 Genomic DNA No translation available.
CH473989 Genomic DNA Translation: EDM10741.1
RefSeqiNP_001129568.1, NM_001136096.1 [Q9WVE9-3]
NP_062100.1, NM_019227.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HS9X-ray2.15P844-855[»]
SMRiQ9WVE9
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi248132, 5 interactors
IntActiQ9WVE9, 3 interactors
MINTiQ9WVE9
STRINGi10116.ENSRNOP00000045513

PTM databases

iPTMnetiQ9WVE9
PhosphoSitePlusiQ9WVE9

Proteomic databases

PaxDbiQ9WVE9
PRIDEiQ9WVE9

Genome annotation databases

EnsembliENSRNOT00000042750; ENSRNOP00000044773; ENSRNOG00000002001 [Q9WVE9-3]
ENSRNOT00000047843; ENSRNOP00000045513; ENSRNOG00000002001 [Q9WVE9-1]
GeneIDi29491
KEGGirno:29491
UCSCiRGD:2935, rat [Q9WVE9-3]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		6453
RGDi2935, Itsn1

Phylogenomic databases

eggNOGiKOG1029, Eukaryota
GeneTreeiENSGT00940000157065
HOGENOMiCLU_002819_2_0_1
InParanoidiQ9WVE9
OMAiRAVYEFN
OrthoDBi807060at2759
PhylomeDBiQ9WVE9

Enzyme and pathway databases

ReactomeiR-RNO-193648, NRAGE signals death through JNK
R-RNO-194840, Rho GTPase cycle
R-RNO-3928662, EPHB-mediated forward signaling
R-RNO-416482, G alpha (12/13) signalling events
R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828, Clathrin-mediated endocytosis

Miscellaneous databases

EvolutionaryTraceiQ9WVE9

Protein Ontology

More...PROi		PR:Q9WVE9

Gene expression databases

BgeeiENSRNOG00000002001, Expressed in skeletal muscle tissue and 20 other tissues

Family and domain databases

CDDicd00052, EH, 2 hits
cd00160, RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008, C2_dom
IPR035892, C2_domain_sf
IPR035899, DBL_dom_sf
IPR000219, DH-domain
IPR011992, EF-hand-dom_pair
IPR018247, EF_Hand_1_Ca_BS
IPR002048, EF_hand_dom
IPR000261, EH_dom
IPR001331, GDS_CDC24_CS
IPR011993, PH-like_dom_sf
IPR001849, PH_domain
IPR036028, SH3-like_dom_sf
IPR001452, SH3_domain
PfamiView protein in Pfam
PF00168, C2, 1 hit
PF12763, EF-hand_4, 2 hits
PF16652, PH_13, 1 hit
PF00621, RhoGEF, 1 hit
PF00018, SH3_1, 2 hits
PF07653, SH3_2, 2 hits
PF14604, SH3_9, 1 hit
PRINTSiPR00452, SH3DOMAIN
SMARTiView protein in SMART
SM00239, C2, 1 hit
SM00054, EFh, 2 hits
SM00027, EH, 2 hits
SM00233, PH, 1 hit
SM00325, RhoGEF, 1 hit
SM00326, SH3, 5 hits
SUPFAMiSSF47473, SSF47473, 2 hits
SSF48065, SSF48065, 1 hit
SSF50044, SSF50044, 5 hits
PROSITEiView protein in PROSITE
PS50004, C2, 1 hit
PS00741, DH_1, 1 hit
PS50010, DH_2, 1 hit
PS00018, EF_HAND_1, 2 hits
PS50222, EF_HAND_2, 2 hits
PS50031, EH, 2 hits
PS50003, PH_DOMAIN, 1 hit
PS50002, SH3, 5 hits

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiITSN1_RAT
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9WVE9
Secondary accession number(s): D3ZV52, F1M823, Q9WVE1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 5, 2018
Last modified: February 10, 2021
This is version 165 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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