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Protein

Protein kinase C and casein kinase substrate in neurons protein 2

Gene

Pacsin2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.7 Publications

GO - Molecular functioni

  • cytoskeletal protein binding Source: MGI
  • identical protein binding Source: MGI
  • lipid binding Source: UniProtKB
  • phosphatidic acid binding Source: UniProtKB
  • phospholipid binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons protein 2
Alternative name(s):
Syndapin-2
Syndapin-II
Gene namesi
Name:Pacsin2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1345153 Pacsin2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40D → K: Slight increase in affinity for membranes. Increases membrane tubulation activity. 1 Publication1
Mutagenesisi50R → D: Slight decrease in affinity for membranes. Nearly abolishes membrane tubulation activity. 1 Publication1
Mutagenesisi124 – 125MM → TT: Nearly abolishes membrane tubulation activity. 1 Publication2
Mutagenesisi189K → E: Decreases affinity for membranes. 1 Publication1
Mutagenesisi478P → L: Loss of DNM1-, SYNJ1- and WASL-binding. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2176817

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001617961 – 486Protein kinase C and casein kinase substrate in neurons protein 2Add BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-acetyllysineCombined sources1
Modified residuei273PhosphoserineBy similarity1
Modified residuei399PhosphoserineCombined sources1
Modified residuei446PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9WVE8
MaxQBiQ9WVE8
PaxDbiQ9WVE8
PRIDEiQ9WVE8

PTM databases

iPTMnetiQ9WVE8
PhosphoSitePlusiQ9WVE8

Expressioni

Tissue specificityi

Widely expressed (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000016664 Expressed in 275 organ(s), highest expression level in interventricular septum
ExpressionAtlasiQ9WVE8 baseline and differential
GenevisibleiQ9WVE8 MM

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4.6 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9WVE8, 3 interactors
MINTiQ9WVE8
STRINGi10090.ENSMUSP00000058320

Structurei

Secondary structure

1486
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9WVE8
SMRiQ9WVE8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WVE8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 282F-BARPROSITE-ProRule annotationAdd BLAST272
Domaini426 – 486SH3PROSITE-ProRule annotationAdd BLAST61

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili25 – 274Add BLAST250

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi362 – 364NPF13
Motifi405 – 407NPF23
Motifi417 – 419NPF33

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain.2 Publications

Sequence similaritiesi

Belongs to the PACSIN family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2856 Eukaryota
ENOG410XRX2 LUCA
GeneTreeiENSGT00510000046376
HOGENOMiHOG000007245
HOVERGENiHBG053486
InParanoidiQ9WVE8
KOiK20123
OMAiSYKNIYR
OrthoDBiEOG091G0AS9
PhylomeDBiQ9WVE8
TreeFamiTF313677

Family and domain databases

CDDicd07679 F-BAR_PACSIN2, 1 hit
cd11998 SH3_PACSIN1-2, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR035743 PACSIN1/PACSIN2_SH3
IPR028521 PACSIN2
IPR037453 PACSIN2_F-BAR
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR23065:SF14 PTHR23065:SF14, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50002 SH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q9WVE8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCGD LMNCLHERAR
60 70 80 90 100
IEKAYAQQLT EWARRWRQLV EKGPQYGTVE KAWIAVMSEA ERVSELHLEV
110 120 130 140 150
KASLMNEDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK
160 170 180 190 200
EVEAAKKAHH TACKEEKLAI SREANSKADP SLNPEQLKKL QDKIEKCKQD
210 220 230 240 250
VLKTKDKYEK SLKELDQTTP QYMENMEQVF EQCQQFEEKR LRFFREVLLE
260 270 280 290 300
VQKHLDLSNV ASYKTIYREL EQSIKAADAV EDLRWFRANH GPGMAMNWPQ
310 320 330 340 350
FEEWSADLNR TLSRREKKKA VDGVTLTGIN QTGDQSGQNK PGSNLSVPSN
360 370 380 390 400
PAQSTQLQSS YNPFEDEDDT GSSISEKEDI KAKNVSSYEK TQTYPTDWSD
410 420 430 440 450
DESNNPFSST DANGDSNPFD EDTTSGTEVR VRALYDYEGQ EHDELSFKAG
460 470 480
DELTKIEDED EQGWCKGRLD SGQVGLYPAN YVEAIQ
Length:486
Mass (Da):55,833
Last modified:November 1, 1999 - v1
Checksum:i66C17ECCC767E0E7
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8W6S4A0A2R8W6S4_MOUSE
Protein kinase C and casein kinase ...
Pacsin2
272Annotation score:
Q3UP40Q3UP40_MOUSE
Protein kinase C and casein kinase ...
Pacsin2
191Annotation score:
A0A2R8W750A0A2R8W750_MOUSE
Protein kinase C and casein kinase ...
Pacsin2
124Annotation score:
A0A338P6P7A0A338P6P7_MOUSE
Protein kinase C and casein kinase ...
Pacsin2
180Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128535 mRNA Translation: AAD41780.1
BC023502 mRNA Translation: AAH23502.1
CCDSiCCDS27701.1
RefSeqiNP_001152981.1, NM_001159509.1
NP_001152982.1, NM_001159510.1
NP_035992.1, NM_011862.3
UniGeneiMm.23978

Genome annotation databases

EnsembliENSMUST00000056177; ENSMUSP00000058320; ENSMUSG00000016664
ENSMUST00000165095; ENSMUSP00000130098; ENSMUSG00000016664
ENSMUST00000171436; ENSMUSP00000131504; ENSMUSG00000016664
ENSMUST00000230679; ENSMUSP00000155481; ENSMUSG00000016664
ENSMUST00000231184; ENSMUSP00000155334; ENSMUSG00000016664
GeneIDi23970
KEGGimmu:23970
UCSCiuc007xba.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128535 mRNA Translation: AAD41780.1
BC023502 mRNA Translation: AAH23502.1
CCDSiCCDS27701.1
RefSeqiNP_001152981.1, NM_001159509.1
NP_001152982.1, NM_001159510.1
NP_035992.1, NM_011862.3
UniGeneiMm.23978

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LLLX-ray3.30A/B16-302[»]
ProteinModelPortaliQ9WVE8
SMRiQ9WVE8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WVE8, 3 interactors
MINTiQ9WVE8
STRINGi10090.ENSMUSP00000058320

Chemistry databases

ChEMBLiCHEMBL2176817

PTM databases

iPTMnetiQ9WVE8
PhosphoSitePlusiQ9WVE8

Proteomic databases

EPDiQ9WVE8
MaxQBiQ9WVE8
PaxDbiQ9WVE8
PRIDEiQ9WVE8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056177; ENSMUSP00000058320; ENSMUSG00000016664
ENSMUST00000165095; ENSMUSP00000130098; ENSMUSG00000016664
ENSMUST00000171436; ENSMUSP00000131504; ENSMUSG00000016664
ENSMUST00000230679; ENSMUSP00000155481; ENSMUSG00000016664
ENSMUST00000231184; ENSMUSP00000155334; ENSMUSG00000016664
GeneIDi23970
KEGGimmu:23970
UCSCiuc007xba.2 mouse

Organism-specific databases

CTDi11252
MGIiMGI:1345153 Pacsin2

Phylogenomic databases

eggNOGiKOG2856 Eukaryota
ENOG410XRX2 LUCA
GeneTreeiENSGT00510000046376
HOGENOMiHOG000007245
HOVERGENiHBG053486
InParanoidiQ9WVE8
KOiK20123
OMAiSYKNIYR
OrthoDBiEOG091G0AS9
PhylomeDBiQ9WVE8
TreeFamiTF313677

Enzyme and pathway databases

ReactomeiR-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiPacsin2 mouse
EvolutionaryTraceiQ9WVE8
PROiPR:Q9WVE8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016664 Expressed in 275 organ(s), highest expression level in interventricular septum
ExpressionAtlasiQ9WVE8 baseline and differential
GenevisibleiQ9WVE8 MM

Family and domain databases

CDDicd07679 F-BAR_PACSIN2, 1 hit
cd11998 SH3_PACSIN1-2, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR035743 PACSIN1/PACSIN2_SH3
IPR028521 PACSIN2
IPR037453 PACSIN2_F-BAR
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR23065:SF14 PTHR23065:SF14, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPACN2_MOUSE
AccessioniPrimary (citable) accession number: Q9WVE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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