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Protein

Single-stranded DNA cytosine deaminase

Gene

Aicda

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.2 Publications

Catalytic activityi

Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.2 Publications

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Zinc; catalyticBy similarity1
Active sitei58Proton donorBy similarity1
Metal bindingi87Zinc; catalyticBy similarity1
Metal bindingi90Zinc; catalyticBy similarity1

GO - Molecular functioni

  • cytidine deaminase activity Source: MGI
  • identical protein binding Source: MGI
  • RNA binding Source: GO_Central
  • ubiquitin protein ligase binding Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processmRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.38 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA cytosine deaminase (EC:3.5.4.382 Publications)
Alternative name(s):
Activation-induced cytidine deaminase
Short name:
AID
Cytidine aminohydrolase
Gene namesi
Name:Aicda
Synonyms:Aid
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1342279 Aicda

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15F → L: Severely decreased cytidine deaminase activity. Loss of mutagenic activity. 1 Publication1
Mutagenesisi56H → R: Loss of the cytidine deaminase activity; when associated with Q-58. 1 Publication1
Mutagenesisi58E → Q: Loss of the cytidine deaminase activity; when associated with R-56. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001716881 – 198Single-stranded DNA cytosine deaminaseAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27Phosphothreonine; by PKABy similarity1
Modified residuei38Phosphoserine; by PKABy similarity1

Post-translational modificationi

Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity (By similarity).By similarity
Probably monoubiquitinated on several residues by RNF126.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9WVE0
PRIDEiQ9WVE0

PTM databases

iPTMnetiQ9WVE0
PhosphoSitePlusiQ9WVE0

Expressioni

Gene expression databases

BgeeiENSMUSG00000040627 Expressed in 60 organ(s), highest expression level in cumulus cell
CleanExiMM_AICDA
ExpressionAtlasiQ9WVE0 baseline and differential
GenevisibleiQ9WVE0 MM

Interactioni

Subunit structurei

Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 (By similarity). Interacts with SUPT6H, TRIM28 and NCL.By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198040, 5 interactors
DIPiDIP-48714N
IntActiQ9WVE0, 54 interactors
MINTiQ9WVE0
STRINGi10090.ENSMUSP00000040524

Structurei

3D structure databases

ProteinModelPortaliQ9WVE0
SMRiQ9WVE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 129CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 26Interaction with SUPT6HBy similarityAdd BLAST25
Regioni39 – 42Important for interaction with CTNNBL1By similarity4
Regioni88 – 116Required for interaction with RNF126By similarityAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1 – 30Bipartite nuclear localization signalBy similarityAdd BLAST30
Motifi183 – 198Nuclear export signalBy similarityAdd BLAST16

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFHW Eukaryota
ENOG4111HYQ LUCA
GeneTreeiENSGT00530000062933
HOGENOMiHOG000033754
HOVERGENiHBG050434
InParanoidiQ9WVE0
KOiK10989
OMAiGYPNLSL
OrthoDBiEOG091G0J2L
PhylomeDBiQ9WVE0
TreeFamiTF331356

Family and domain databases

InterProiView protein in InterPro
IPR016192 APOBEC/CMP_deaminase_Zn-bd
IPR013158 APOBEC_N
IPR002125 CMP_dCMP_dom
IPR016193 Cytidine_deaminase-like
PfamiView protein in Pfam
PF08210 APOBEC_N, 1 hit
SUPFAMiSSF53927 SSF53927, 1 hit
PROSITEiView protein in PROSITE
PS00903 CYT_DCMP_DEAMINASES_1, 1 hit
PS51747 CYT_DCMP_DEAMINASES_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9WVE0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDSLLMKQKK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SCSLDFGHLR
60 70 80 90 100
NKSGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVAEFLRW
110 120 130 140 150
NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIGIMT FKDYFYCWNT
160 170 180 190
FVENRERTFK AWEGLHENSV RLTRQLRRIL LPLYEVDDLR DAFRMLGF
Length:198
Mass (Da):24,031
Last modified:November 1, 1999 - v1
Checksum:i18A3BA10CA54BEB2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E0CXQ9E0CXQ9_MOUSE
Single-stranded DNA cytosine deamin...
Aicda
60Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132979 mRNA Translation: AAD41793.1
CCDSiCCDS20497.1
RefSeqiNP_033775.1, NM_009645.2
UniGeneiMm.391503

Genome annotation databases

EnsembliENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627
GeneIDi11628
KEGGimmu:11628
UCSCiuc009dpj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132979 mRNA Translation: AAD41793.1
CCDSiCCDS20497.1
RefSeqiNP_033775.1, NM_009645.2
UniGeneiMm.391503

3D structure databases

ProteinModelPortaliQ9WVE0
SMRiQ9WVE0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198040, 5 interactors
DIPiDIP-48714N
IntActiQ9WVE0, 54 interactors
MINTiQ9WVE0
STRINGi10090.ENSMUSP00000040524

PTM databases

iPTMnetiQ9WVE0
PhosphoSitePlusiQ9WVE0

Proteomic databases

PaxDbiQ9WVE0
PRIDEiQ9WVE0

Protocols and materials databases

DNASUi11628
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627
GeneIDi11628
KEGGimmu:11628
UCSCiuc009dpj.1 mouse

Organism-specific databases

CTDi57379
MGIiMGI:1342279 Aicda

Phylogenomic databases

eggNOGiENOG410IFHW Eukaryota
ENOG4111HYQ LUCA
GeneTreeiENSGT00530000062933
HOGENOMiHOG000033754
HOVERGENiHBG050434
InParanoidiQ9WVE0
KOiK10989
OMAiGYPNLSL
OrthoDBiEOG091G0J2L
PhylomeDBiQ9WVE0
TreeFamiTF331356

Enzyme and pathway databases

BRENDAi3.5.4.38 3474

Miscellaneous databases

PROiPR:Q9WVE0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040627 Expressed in 60 organ(s), highest expression level in cumulus cell
CleanExiMM_AICDA
ExpressionAtlasiQ9WVE0 baseline and differential
GenevisibleiQ9WVE0 MM

Family and domain databases

InterProiView protein in InterPro
IPR016192 APOBEC/CMP_deaminase_Zn-bd
IPR013158 APOBEC_N
IPR002125 CMP_dCMP_dom
IPR016193 Cytidine_deaminase-like
PfamiView protein in Pfam
PF08210 APOBEC_N, 1 hit
SUPFAMiSSF53927 SSF53927, 1 hit
PROSITEiView protein in PROSITE
PS00903 CYT_DCMP_DEAMINASES_1, 1 hit
PS51747 CYT_DCMP_DEAMINASES_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAICDA_MOUSE
AccessioniPrimary (citable) accession number: Q9WVE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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