UniProtKB - Q9WVC8 (S26A3_MOUSE)
Protein
Chloride anion exchanger
Gene
Slc26a3
Organism
Mus musculus (Mouse)
Status
Functioni
Chloride/bicarbonate exchanger. Mediates the efficient absorption of chloride ions in the colon, participating in fluid homeostasis. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation.2 Publications
GO - Molecular functioni
- anion:anion antiporter activity Source: MGI
- bicarbonate transmembrane transporter activity Source: UniProtKB
- chloride transmembrane transporter activity Source: UniProtKB
- oxalate transmembrane transporter activity Source: GO_Central
- secondary active sulfate transmembrane transporter activity Source: InterPro
- sulfate transmembrane transporter activity Source: GO_Central
GO - Biological processi
- cellular response to cAMP Source: UniProtKB
- intracellular pH elevation Source: UniProtKB
- membrane hyperpolarization Source: UniProtKB
- sperm capacitation Source: UniProtKB
Keywordsi
Biological process | Antiport, Transport |
Ligand | Chloride |
Enzyme and pathway databases
Reactomei | R-MMU-427601, Multifunctional anion exchangers |
Protein family/group databases
TCDBi | 2.A.53.2.3, the sulfate permease (sulp) family |
Names & Taxonomyi
Protein namesi | Recommended name: Chloride anion exchangerAlternative name(s): Down-regulated in adenoma Short name: Protein DRA Solute carrier family 26 member 3 |
Gene namesi | Name:Slc26a3 Synonyms:Dra |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107181, Slc26a3 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- Membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Localized in sperm membranes. Midpiece of sperm tail. Colocalizes with CFTR at the midpiece of sperm tail.
Plasma Membrane
- apical plasma membrane Source: MGI
- brush border membrane Source: MGI
- integral component of plasma membrane Source: MGI
Other locations
- membrane Source: UniProtKB
- sperm midpiece Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 71 | CytoplasmicCuratedAdd BLAST | 71 | |
Transmembranei | 72 – 92 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 93 | ExtracellularCurated | 1 | |
Transmembranei | 94 – 114 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 115 – 124 | CytoplasmicCurated | 10 | |
Transmembranei | 125 – 145 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 146 – 176 | ExtracellularCuratedAdd BLAST | 31 | |
Transmembranei | 177 – 197 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 198 – 201 | CytoplasmicCurated | 4 | |
Transmembranei | 202 – 222 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 223 – 250 | ExtracellularCuratedAdd BLAST | 28 | |
Transmembranei | 251 – 271 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 272 – 278 | CytoplasmicCurated | 7 | |
Transmembranei | 279 – 299 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 300 – 335 | ExtracellularCuratedAdd BLAST | 36 | |
Transmembranei | 336 – 356 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 357 – 367 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 368 – 388 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 389 – 404 | ExtracellularCuratedAdd BLAST | 16 | |
Transmembranei | 405 – 425 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 426 – 462 | CytoplasmicCuratedAdd BLAST | 37 | |
Transmembranei | 463 – 483 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 484 – 757 | ExtracellularCuratedAdd BLAST | 274 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080162 | 1 – 757 | Chloride anion exchangerAdd BLAST | 757 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 149 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 161 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N-glycosylation is required for efficient cell surface expression, and protection from proteolytic degradation.By similarity
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | Q9WVC8 |
PaxDbi | Q9WVC8 |
PRIDEi | Q9WVC8 |
ProteomicsDBi | 256672 |
PTM databases
GlyGeni | Q9WVC8, 2 sites |
Expressioni
Tissue specificityi
Expressed in spermatogenic cells.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000001225, Expressed in left colon and 100 other tissues |
ExpressionAtlasi | Q9WVC8, baseline and differential |
Genevisiblei | Q9WVC8, MM |
Interactioni
Subunit structurei
Interacts with PDZK1 (By similarity).
Interacts with CFTR, SLC26A6 and SLC9A3R1.
By similarity1 PublicationBinary interactionsi
Hide detailsQ9WVC8
With | #Exp. | IntAct |
---|---|---|
Cftr [P26361] | 3 | EBI-6895537,EBI-6115317 |
Slc26a6 [Q8CIW6] | 2 | EBI-6895537,EBI-6895517 |
Slc9a3r1 [P70441] | 2 | EBI-6895537,EBI-1184085 |
Protein-protein interaction databases
IntActi | Q9WVC8, 3 interactors |
STRINGi | 10090.ENSMUSP00000001254 |
Miscellaneous databases
RNActi | Q9WVC8, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9WVC8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 518 – 713 | STASPROSITE-ProRule annotationAdd BLAST | 196 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0236, Eukaryota |
GeneTreei | ENSGT01000000214410 |
HOGENOMi | CLU_003182_9_4_1 |
InParanoidi | Q9WVC8 |
OMAi | MLMQFTE |
OrthoDBi | 690428at2759 |
PhylomeDBi | Q9WVC8 |
TreeFami | TF313784 |
Family and domain databases
Gene3Di | 3.30.750.24, 1 hit |
InterProi | View protein in InterPro IPR018045, S04_transporter_CS IPR011547, SLC26A/SulP_dom IPR001902, SLC26A/SulP_fam IPR030321, SLC26A3 IPR002645, STAS_dom IPR036513, STAS_dom_sf |
PANTHERi | PTHR11814, PTHR11814, 1 hit PTHR11814:SF19, PTHR11814:SF19, 1 hit |
Pfami | View protein in Pfam PF01740, STAS, 1 hit PF00916, Sulfate_transp, 1 hit |
SUPFAMi | SSF52091, SSF52091, 1 hit |
TIGRFAMsi | TIGR00815, sulP, 1 hit |
PROSITEi | View protein in PROSITE PS01130, SLC26A, 1 hit PS50801, STAS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9WVC8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEAIGNQYV VARPVYSTKT FGEEFKKTHR HHKTFLDHLK GCCSCSSQKA
60 70 80 90 100
KKIALSLFPI ASWLPAYKIK EWLLSDIVSG ISTGLVAVLQ GLAFALLVNI
110 120 130 140 150
PPAYGLYAAF FPVITYFFLG TSRHISVGPF PVLSMMVGVV VTRVVSDPNA
160 170 180 190 200
SSELSSSSTE NDSFIEEKVM VAASVTVLSG IIQLLLGVLQ VGFVVIYLSE
210 220 230 240 250
SLISGFTTAA AIHVLVSQLK FMLQLPVPAY SDPFSIFKVL ESVFTQIQKT
260 270 280 290 300
NIADLVTSVI ILVVVFVFKE INQRYRSKLP VPIPIELIMT VIATGVSYGC
310 320 330 340 350
NFEDRFGVAV VGNMSLGFQP PITPSVEVFQ DTIGDSFGIA IVGFAVAFSV
360 370 380 390 400
ASVYSLKYDY PIDGNQELIA LGVSNIFTGA FKGFAGSTAL SRSGVQESTG
410 420 430 440 450
GKTQVAGLLS AVIVLIVIVA IGFLLQPLQK SVLAALALGN LKGMLMQFAE
460 470 480 490 500
IGRLWKKDKY DCLIWIMTFI FAIVLGLGLG LAASVAFQLL TIVFRTQFPK
510 520 530 540 550
CSTLANVGRS NIYKNKKNYA EVYEPEGVKI FRCPSPIYFA NIGFFKQKLI
560 570 580 590 600
DAVGFSPLRI LRKRNKALKK IRKLQKRGLI QMTPKGFICT SDGFKDSDEE
610 620 630 640 650
LDNNQIEELD QPINTTDLPF DIDWNGDLPL NITIPKISLH SLILDFSAVS
660 670 680 690 700
FLDVSSMRGL RTILQEFIRI KVDVYIVGTD DDFIDKLARC EFFDDEVTDS
710 720 730 740 750
IFFLTIHDAI LHILMKKDYS TSKFNSSQEK ERKFDFTINT NGGLRNRECQ
VPVETKF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QAZ3 | E9QAZ3_MOUSE | Chloride anion exchanger | Slc26a3 | 235 | Annotation score: | ||
E9PY22 | E9PY22_MOUSE | Chloride anion exchanger | Slc26a3 | 58 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF136751 mRNA Translation: AAD42784.1 BC139271 mRNA Translation: AAI39272.1 BC139273 mRNA Translation: AAI39274.1 |
CCDSi | CCDS25863.1 |
RefSeqi | NP_067328.1, NM_021353.3 XP_017170439.1, XM_017314950.1 |
Genome annotation databases
Ensembli | ENSMUST00000001254; ENSMUSP00000001254; ENSMUSG00000001225 |
GeneIDi | 13487 |
KEGGi | mmu:13487 |
UCSCi | uc007nhj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF136751 mRNA Translation: AAD42784.1 BC139271 mRNA Translation: AAI39272.1 BC139273 mRNA Translation: AAI39274.1 |
CCDSi | CCDS25863.1 |
RefSeqi | NP_067328.1, NM_021353.3 XP_017170439.1, XM_017314950.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5DOW | X-ray | 1.70 | B/D/F/H | 563-583 | [»] | |
SMRi | Q9WVC8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q9WVC8, 3 interactors |
STRINGi | 10090.ENSMUSP00000001254 |
Protein family/group databases
TCDBi | 2.A.53.2.3, the sulfate permease (sulp) family |
PTM databases
GlyGeni | Q9WVC8, 2 sites |
Proteomic databases
MaxQBi | Q9WVC8 |
PaxDbi | Q9WVC8 |
PRIDEi | Q9WVC8 |
ProteomicsDBi | 256672 |
Protocols and materials databases
Antibodypediai | 31383, 80 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000001254; ENSMUSP00000001254; ENSMUSG00000001225 |
GeneIDi | 13487 |
KEGGi | mmu:13487 |
UCSCi | uc007nhj.2, mouse |
Organism-specific databases
CTDi | 1811 |
MGIi | MGI:107181, Slc26a3 |
Phylogenomic databases
eggNOGi | KOG0236, Eukaryota |
GeneTreei | ENSGT01000000214410 |
HOGENOMi | CLU_003182_9_4_1 |
InParanoidi | Q9WVC8 |
OMAi | MLMQFTE |
OrthoDBi | 690428at2759 |
PhylomeDBi | Q9WVC8 |
TreeFami | TF313784 |
Enzyme and pathway databases
Reactomei | R-MMU-427601, Multifunctional anion exchangers |
Miscellaneous databases
BioGRID-ORCSi | 13487, 1 hit in 52 CRISPR screens |
ChiTaRSi | Slc26a3, mouse |
PROi | PR:Q9WVC8 |
RNActi | Q9WVC8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001225, Expressed in left colon and 100 other tissues |
ExpressionAtlasi | Q9WVC8, baseline and differential |
Genevisiblei | Q9WVC8, MM |
Family and domain databases
Gene3Di | 3.30.750.24, 1 hit |
InterProi | View protein in InterPro IPR018045, S04_transporter_CS IPR011547, SLC26A/SulP_dom IPR001902, SLC26A/SulP_fam IPR030321, SLC26A3 IPR002645, STAS_dom IPR036513, STAS_dom_sf |
PANTHERi | PTHR11814, PTHR11814, 1 hit PTHR11814:SF19, PTHR11814:SF19, 1 hit |
Pfami | View protein in Pfam PF01740, STAS, 1 hit PF00916, Sulfate_transp, 1 hit |
SUPFAMi | SSF52091, SSF52091, 1 hit |
TIGRFAMsi | TIGR00815, sulP, 1 hit |
PROSITEi | View protein in PROSITE PS01130, SLC26A, 1 hit PS50801, STAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S26A3_MOUSE | |
Accessioni | Q9WVC8Primary (citable) accession number: Q9WVC8 Secondary accession number(s): B2RTD9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 5, 2002 |
Last sequence update: | November 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families