UniProtKB - Q9WVA3 (BUB3_MOUSE)
Protein
Mitotic checkpoint protein BUB3
Gene
Bub3
Organism
Mus musculus (Mouse)
Status
Functioni
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1 (By similarity). Regulates chromosome segregation during oocyte meiosis.By similarity1 Publication
GO - Molecular functioni
- ubiquitin binding Source: GO_Central
GO - Biological processi
- attachment of spindle microtubules to kinetochore Source: MGI
- cell division Source: UniProtKB-KW
- chromosome segregation Source: MGI
- meiotic cell cycle Source: UniProtKB-KW
- mitotic sister chromatid segregation Source: MGI
- mitotic spindle assembly checkpoint Source: GO_Central
- protein localization to kinetochore Source: MGI
- regulation of chromosome segregation Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-MMU-179409, APC-Cdc20 mediated degradation of Nek2A R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Mitotic checkpoint protein BUB3Alternative name(s): WD repeat type I transmembrane protein A72.5 |
Gene namesi | Name:Bub3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1343463, Bub3 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- kinetochore 1 Publication
Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.By similarity
Nucleus
- mitotic checkpoint complex Source: GO_Central
- nucleoplasm Source: MGI
Other locations
- bub1-bub3 complex Source: GO_Central
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- kinetochore Source: MGI
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050892 | 1 – 326 | Mitotic checkpoint protein BUB3Add BLAST | 326 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 179 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 211 | PhosphoserineBy similarity | 1 | |
Cross-linki | 216 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity |
Post-translational modificationi
Poly-ADP-ribosylated by PARP1.
Keywords - PTMi
Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9WVA3 |
MaxQBi | Q9WVA3 |
PaxDbi | Q9WVA3 |
PeptideAtlasi | Q9WVA3 |
PRIDEi | Q9WVA3 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9WVA3 |
PTM databases
iPTMneti | Q9WVA3 |
PhosphoSitePlusi | Q9WVA3 |
SwissPalmi | Q9WVA3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000066979, Expressed in vestibular epithelium and 314 other tissues |
Genevisiblei | Q9WVA3, MM |
Interactioni
Subunit structurei
Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1.
Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3 (By similarity).
By similarityGO - Molecular functioni
- ubiquitin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 198406, 9 interactors |
ComplexPortali | CPX-3968, Mitotic Checkpoint Complex |
IntActi | Q9WVA3, 4 interactors |
MINTi | Q9WVA3 |
STRINGi | 10090.ENSMUSP00000081547 |
Miscellaneous databases
RNActi | Q9WVA3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 5 – 43 | WD 1Add BLAST | 39 | |
Repeati | 46 – 83 | WD 2Add BLAST | 38 | |
Repeati | 86 – 124 | WD 3Add BLAST | 39 | |
Repeati | 128 – 163 | WD 4Add BLAST | 36 | |
Repeati | 223 – 262 | WD 5Add BLAST | 40 |
Sequence similaritiesi
Belongs to the WD repeat BUB3 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG1036, Eukaryota |
GeneTreei | ENSGT00950000183091 |
HOGENOMi | CLU_038526_0_0_1 |
InParanoidi | Q9WVA3 |
OrthoDBi | 1048963at2759 |
TreeFami | TF105454 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 3 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 2 hits PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9WVA3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTGSNEFKLN QPPEDGISSV KFSPNTSQFL LVSSWDTSVR LYDVPANSMR
60 70 80 90 100
LKYQHTGAVL DCAFYDPTHA WSGGLDHQLK MHDLNTDQEN LVGTHDAPIR
110 120 130 140 150
CVEYCPEVNV MVTGSWDQTV KLWDPRTPCN AGTFSQPEKV YTLSVSGDRL
160 170 180 190 200
IVGTAGRRVL VWDLRNMGYV QQRRESSLKY QTRCIRAFPN KQGYVLSSIE
210 220 230 240 250
GRVAVEYLDP SPEVQKKKYA FKCHRLKENN IEQIYPVNAI SFHNIHNTFA
260 270 280 290 300
TGGSDGFVNI WDPFNKKRLC QFHRYPTSIA SLAFSNDGTT LAIASSYMYE
310 320
MDDTEHPEDG IFIRQVTDAE TKPKST
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LHA2 | A0A140LHA2_MOUSE | Mitotic checkpoint protein BUB3 | Bub3 | 324 | Annotation score: | ||
A0A140LJ21 | A0A140LJ21_MOUSE | Mitotic checkpoint protein BUB3 | Bub3 | 220 | Annotation score: | ||
A0A140LIM5 | A0A140LIM5_MOUSE | Mitotic checkpoint protein BUB3 | Bub3 | 184 | Annotation score: | ||
A0A140LI47 | A0A140LI47_MOUSE | Mitotic checkpoint protein BUB3 | Bub3 | 100 | Annotation score: |
Sequence cautioni
The sequence AAB39606 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | C → W in AAB39606 (Ref. 2) Curated | 1 | |
Sequence conflicti | 156 | G → A in AAB39606 (Ref. 2) Curated | 1 | |
Sequence conflicti | 165 | R → W in AAD38038 (PubMed:10411903).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF149822 mRNA Translation: AAD38038.1 U67327 mRNA Translation: AAB39606.1 Frameshift. AK088534 mRNA Translation: BAC40409.1 BC025089 mRNA Translation: AAH25089.1 |
CCDSi | CCDS40160.1 |
RefSeqi | NP_001304279.1, NM_001317350.1 NP_033904.2, NM_009774.4 |
Genome annotation databases
Ensembli | ENSMUST00000084502; ENSMUSP00000081547; ENSMUSG00000066979 |
GeneIDi | 12237 |
KEGGi | mmu:12237 |
UCSCi | uc009kbq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF149822 mRNA Translation: AAD38038.1 U67327 mRNA Translation: AAB39606.1 Frameshift. AK088534 mRNA Translation: BAC40409.1 BC025089 mRNA Translation: AAH25089.1 |
CCDSi | CCDS40160.1 |
RefSeqi | NP_001304279.1, NM_001317350.1 NP_033904.2, NM_009774.4 |
3D structure databases
SMRi | Q9WVA3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198406, 9 interactors |
ComplexPortali | CPX-3968, Mitotic Checkpoint Complex |
IntActi | Q9WVA3, 4 interactors |
MINTi | Q9WVA3 |
STRINGi | 10090.ENSMUSP00000081547 |
PTM databases
iPTMneti | Q9WVA3 |
PhosphoSitePlusi | Q9WVA3 |
SwissPalmi | Q9WVA3 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9WVA3 |
Proteomic databases
EPDi | Q9WVA3 |
MaxQBi | Q9WVA3 |
PaxDbi | Q9WVA3 |
PeptideAtlasi | Q9WVA3 |
PRIDEi | Q9WVA3 |
Protocols and materials databases
Antibodypediai | 1213, 357 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000084502; ENSMUSP00000081547; ENSMUSG00000066979 |
GeneIDi | 12237 |
KEGGi | mmu:12237 |
UCSCi | uc009kbq.1, mouse |
Organism-specific databases
CTDi | 9184 |
MGIi | MGI:1343463, Bub3 |
Phylogenomic databases
eggNOGi | KOG1036, Eukaryota |
GeneTreei | ENSGT00950000183091 |
HOGENOMi | CLU_038526_0_0_1 |
InParanoidi | Q9WVA3 |
OrthoDBi | 1048963at2759 |
TreeFami | TF105454 |
Enzyme and pathway databases
Reactomei | R-MMU-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-MMU-179409, APC-Cdc20 mediated degradation of Nek2A R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 12237, 7 hits in 17 CRISPR screens |
ChiTaRSi | Bub3, mouse |
PROi | PR:Q9WVA3 |
RNActi | Q9WVA3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000066979, Expressed in vestibular epithelium and 314 other tissues |
Genevisiblei | Q9WVA3, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 3 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 2 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BUB3_MOUSE | |
Accessioni | Q9WVA3Primary (citable) accession number: Q9WVA3 Secondary accession number(s): P97397, Q6ZWM5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families