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Protein

Nucleoside diphosphate kinase, mitochondrial

Gene

Nme4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis. Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity. Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration. Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria. In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (By similarity).By similarity

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44ATPBy similarity1
Binding sitei92ATPBy similarity1
Binding sitei120ATPBy similarity1
Binding sitei126ATPBy similarity1
Binding sitei137ATPBy similarity1
Binding sitei147ATPBy similarity1
Active sitei150Pros-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid transport, Nucleotide metabolism, Transport
LigandATP-binding, Lipid-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-499943 Interconversion of nucleotide di- and triphosphates

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase, mitochondrial (EC:2.7.4.6)
Short name:
NDK
Short name:
NDP kinase, mitochondrial
Alternative name(s):
Nucleoside diphosphate kinase D
Short name:
NDPKD
nm23-M4
Gene namesi
Name:Nme4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1931148 Nme4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionBy similarityAdd BLAST32
ChainiPRO_000001943333 – 186Nucleoside diphosphate kinase, mitochondrialAdd BLAST154

Proteomic databases

EPDiQ9WV84
MaxQBiQ9WV84
PaxDbiQ9WV84
PRIDEiQ9WV84

PTM databases

PhosphoSitePlusiQ9WV84

Expressioni

Tissue specificityi

Expressed in the base region of the oxyntic and pyloric mucosae.1 Publication

Gene expression databases

BgeeiENSMUSG00000024177 Expressed in 176 organ(s), highest expression level in dorsal pancreas
CleanExiMM_NME4
GenevisibleiQ9WV84 MM

Interactioni

Subunit structurei

Homohexamer (By similarity). Interacts with OPA1 (By similarity). Interacts with CAPN8 (PubMed:16476741).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025007

Structurei

3D structure databases

ProteinModelPortaliQ9WV84
SMRiQ9WV84
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0888 Eukaryota
COG0105 LUCA
GeneTreeiENSGT00760000119146
HOVERGENiHBG000423
InParanoidiQ9WV84
KOiK00940
OMAiVVMVWEG
OrthoDBiEOG091G0PLD
PhylomeDBiQ9WV84
TreeFamiTF106373

Family and domain databases

Gene3Di3.30.70.141, 1 hit
HAMAPiMF_00451 NDP_kinase, 1 hit
InterProiView protein in InterPro
IPR034907 NDK-like_dom
IPR036850 NDK-like_dom_sf
IPR001564 Nucleoside_diP_kinase
IPR023005 Nucleoside_diP_kinase_AS
PfamiView protein in Pfam
PF00334 NDK, 1 hit
PRINTSiPR01243 NUCDPKINASE
SMARTiView protein in SMART
SM00562 NDK, 1 hit
SUPFAMiSSF54919 SSF54919, 1 hit
PROSITEiView protein in PROSITE
PS00469 NDP_KINASES, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WV84-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSLFGRVAA LRALLCGPRF QCLLVRPSSG GPPWPQERTL VAVKPDGVQR
60 70 80 90 100
RLVGTVIQRF ERRGFKLVGM KMLQAPESIL AEHYRDLQRK PFYPALISYM
110 120 130 140 150
SSGPVVAMVW EGPNVVHISR AMIGHTDSTE AAPGTIRGDF SVHISRNVIH
160 170 180
ASDSVDGAQR EIELWFQSSE LLNWADGGHH SSCYPA
Length:186
Mass (Da):20,549
Last modified:November 1, 1999 - v1
Checksum:i472A32D65C0B25A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153451 mRNA Translation: AAD38977.1
AF288690 mRNA Translation: AAG02200.1
AF288692 Genomic DNA Translation: AAG02202.1
BC027277 mRNA Translation: AAH27277.1
CCDSiCCDS28544.1
RefSeqiNP_062705.1, NM_019731.1
UniGeneiMm.41787

Genome annotation databases

EnsembliENSMUST00000025007; ENSMUSP00000025007; ENSMUSG00000024177
GeneIDi56520
KEGGimmu:56520
UCSCiuc008bdh.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153451 mRNA Translation: AAD38977.1
AF288690 mRNA Translation: AAG02200.1
AF288692 Genomic DNA Translation: AAG02202.1
BC027277 mRNA Translation: AAH27277.1
CCDSiCCDS28544.1
RefSeqiNP_062705.1, NM_019731.1
UniGeneiMm.41787

3D structure databases

ProteinModelPortaliQ9WV84
SMRiQ9WV84
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025007

PTM databases

PhosphoSitePlusiQ9WV84

Proteomic databases

EPDiQ9WV84
MaxQBiQ9WV84
PaxDbiQ9WV84
PRIDEiQ9WV84

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025007; ENSMUSP00000025007; ENSMUSG00000024177
GeneIDi56520
KEGGimmu:56520
UCSCiuc008bdh.1 mouse

Organism-specific databases

CTDi4833
MGIiMGI:1931148 Nme4

Phylogenomic databases

eggNOGiKOG0888 Eukaryota
COG0105 LUCA
GeneTreeiENSGT00760000119146
HOVERGENiHBG000423
InParanoidiQ9WV84
KOiK00940
OMAiVVMVWEG
OrthoDBiEOG091G0PLD
PhylomeDBiQ9WV84
TreeFamiTF106373

Enzyme and pathway databases

ReactomeiR-MMU-499943 Interconversion of nucleotide di- and triphosphates

Miscellaneous databases

PROiPR:Q9WV84
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024177 Expressed in 176 organ(s), highest expression level in dorsal pancreas
CleanExiMM_NME4
GenevisibleiQ9WV84 MM

Family and domain databases

Gene3Di3.30.70.141, 1 hit
HAMAPiMF_00451 NDP_kinase, 1 hit
InterProiView protein in InterPro
IPR034907 NDK-like_dom
IPR036850 NDK-like_dom_sf
IPR001564 Nucleoside_diP_kinase
IPR023005 Nucleoside_diP_kinase_AS
PfamiView protein in Pfam
PF00334 NDK, 1 hit
PRINTSiPR01243 NUCDPKINASE
SMARTiView protein in SMART
SM00562 NDK, 1 hit
SUPFAMiSSF54919 SSF54919, 1 hit
PROSITEiView protein in PROSITE
PS00469 NDP_KINASES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNDKM_MOUSE
AccessioniPrimary (citable) accession number: Q9WV84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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