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Protein

Sorting nexin-1

Gene

Snx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1). Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking (By similarity).By similarity

Miscellaneous

Binds phosphatidylinositol 3-phosphate (PtdIns-3P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2) in liposome-based assays. Can bind PtdIns(3,4,5)P3 in protein:lipid overlay assays, but not in liposome-based assays (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei188Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei214Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei238Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-1
Gene namesi
Name:Snx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1928395 Snx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice are born at the expected Mendelian ratio and are fertile. Mice lacking both Snx1 and Snx2 die during embryonic development, around 9.5 and 11.5 dpc.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002138361 – 522Sorting nexin-1Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineCombined sources1
Modified residuei39PhosphoserineCombined sources1
Modified residuei41PhosphothreonineCombined sources1
Modified residuei48PhosphothreonineBy similarity1
Modified residuei58PhosphoserineCombined sources1
Modified residuei72PhosphoserineBy similarity1
Modified residuei188PhosphoserineCombined sources1
Modified residuei237N6-acetyllysineBy similarity1
Modified residuei280PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9WV80
MaxQBiQ9WV80
PaxDbiQ9WV80
PeptideAtlasiQ9WV80
PRIDEiQ9WV80

PTM databases

iPTMnetiQ9WV80
PhosphoSitePlusiQ9WV80
SwissPalmiQ9WV80

Expressioni

Gene expression databases

CleanExiMM_SNX1

Interactioni

Subunit structurei

Predominantly forms heterodimers with BAR domain-containing sorting nexins SNX5, SNX6 and SNX32 (By similarity). Can self-associate to form homodimers (PubMed:11726276). The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS) and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. Interacts with SNX5, SNX6, SNX32, VPS26A, VPS29, VPS35, DRD5, DENND5A, KALRN, RHOG (GDP-bound form). The interaction with SNX2 is reported controversially. Interacts with DNAJC13; prevented by presence of HGS. Interacts with HGS (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207981, 2 interactors
IntActiQ9WV80, 2 interactors
MINTiQ9WV80
STRINGi10090.ENSMUSP00000034946

Structurei

3D structure databases

ProteinModelPortaliQ9WV80
SMRiQ9WV80
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 272PXPROSITE-ProRule annotationAdd BLAST130
Domaini302 – 522BARAdd BLAST221

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni281 – 298Membrane-binding amphipathic helixBy similarityAdd BLAST18

Domaini

The BAR domain is able to sense membrane curvature upon dimerization. Membrane remodeling seems to implicate insertion of a N-terminal amphipatric helix (AH) in the membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated

Phylogenomic databases

eggNOGiKOG2273 Eukaryota
COG5391 LUCA
HOGENOMiHOG000293327
HOVERGENiHBG000618
InParanoidiQ9WV80
KOiK17917
PhylomeDBiQ9WV80

Family and domain databases

CDDicd07281 PX_SNX1, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR034901 PX_SNX1
IPR028660 SNX1
IPR039358 Sorting_nexin-like
IPR005329 Sorting_nexin_N
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF129 PTHR10555:SF129, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF03700 Sorting_nexin, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9WV80-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGGGGCSA SERLPPPFPG MDPESEGAAG ASEPEAGDSD TEGEDIFTGA
60 70 80 90 100
AAATKPQSPK KTTSLFPIKN GSKENGIHED QDQEPQDLFA DATVELSLDS
110 120 130 140 150
TQNNQKTMPG KTLTSHSPQE ATNSPKPQPS YEELEEEEQE DQFDLTVGIT
160 170 180 190 200
DPEKIGDGMN AYVAYKVTTQ TSLPMFRSRQ FAVKRRFSDF LGLYEKLSEK
210 220 230 240 250
HSQNGFIVPP PPEKSLIGMT KVKVGKEDSS SAEFLEKRRA ALERYLQRIV
260 270 280 290 300
NHPTMLQDPD VREFLEKEEL PRAVGTQALS GAGLLKMFNK ATDAVSKMTI
310 320 330 340 350
KMNESDIWFE EKLQEVECEE QRLRKLHAVV ETLVNHRKEL ALNTALFAKS
360 370 380 390 400
LAMLGSSEDN TALSRALSQL AEVEEKIEQL HQEQANNDSF LLAELLSDYI
410 420 430 440 450
RLLAIVRAAF DQRMKTWQRW QDAQATLQKK RESEARLLWA NKPDKLQQAK
460 470 480 490 500
DEITEWESRV TQYERDFERI STVVRKEVTR FEKEKSKDFK NHVMKYLETL
510 520
LHSQQQLAKY WEAFLPEAKA IS
Length:522
Mass (Da):58,952
Last modified:November 1, 1999 - v1
Checksum:i1EFC06B3EA551311
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q6NZD2Q6NZD2_MOUSE
Sorting nexin-1
Snx1
521Annotation score:
D3YWH1D3YWH1_MOUSE
Sorting nexin-1
Snx1
192Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31A → G in BAB20283 (PubMed:11726276).Curated1
Sequence conflicti117S → P in BAB20283 (PubMed:11726276).Curated1
Sequence conflicti124S → C in BAB20283 (PubMed:11726276).Curated1
Sequence conflicti138Missing in BAB20283 (PubMed:11726276).Curated1
Sequence conflicti389S → F in BAB20283 (PubMed:11726276).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF154120 mRNA Translation: AAD38805.1
AB019214 mRNA Translation: BAB20283.1
RefSeqiNP_062701.2, NM_019727.2
UniGeneiMm.271891

Genome annotation databases

GeneIDi56440
KEGGimmu:56440

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF154120 mRNA Translation: AAD38805.1
AB019214 mRNA Translation: BAB20283.1
RefSeqiNP_062701.2, NM_019727.2
UniGeneiMm.271891

3D structure databases

ProteinModelPortaliQ9WV80
SMRiQ9WV80
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207981, 2 interactors
IntActiQ9WV80, 2 interactors
MINTiQ9WV80
STRINGi10090.ENSMUSP00000034946

PTM databases

iPTMnetiQ9WV80
PhosphoSitePlusiQ9WV80
SwissPalmiQ9WV80

Proteomic databases

EPDiQ9WV80
MaxQBiQ9WV80
PaxDbiQ9WV80
PeptideAtlasiQ9WV80
PRIDEiQ9WV80

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi56440
KEGGimmu:56440

Organism-specific databases

CTDi6642
MGIiMGI:1928395 Snx1

Phylogenomic databases

eggNOGiKOG2273 Eukaryota
COG5391 LUCA
HOGENOMiHOG000293327
HOVERGENiHBG000618
InParanoidiQ9WV80
KOiK17917
PhylomeDBiQ9WV80

Miscellaneous databases

ChiTaRSiSnx1 mouse
PROiPR:Q9WV80
SOURCEiSearch...

Gene expression databases

CleanExiMM_SNX1

Family and domain databases

CDDicd07281 PX_SNX1, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR034901 PX_SNX1
IPR028660 SNX1
IPR039358 Sorting_nexin-like
IPR005329 Sorting_nexin_N
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF129 PTHR10555:SF129, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF03700 Sorting_nexin, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSNX1_MOUSE
AccessioniPrimary (citable) accession number: Q9WV80
Secondary accession number(s): Q9EQZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: September 12, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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