UniProtKB - Q9WV32 (ARC1B_MOUSE)
Protein
Actin-related protein 2/3 complex subunit 1B
Gene
Arpc1b
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).By similarity
GO - Molecular functioni
- actin filament binding Source: GO_Central
- protein-containing complex binding Source: MGI
- structural constituent of cytoskeleton Source: MGI
GO - Biological processi
- Arp2/3 complex-mediated actin nucleation Source: MGI
- response to estradiol Source: Ensembl
- response to estrogen Source: Ensembl
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-3928662, EPHB-mediated forward signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Actin-related protein 2/3 complex subunit 1BAlternative name(s): Arp2/3 complex 41 kDa subunit p41-ARC |
Gene namesi | Name:Arpc1b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1343142, Arpc1b |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Nucleus
- Nucleus By similarity
Cytoskeleton
- Arp2/3 protein complex Source: MGI
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-KW
- tubulobulbar complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050856 | 1 – 372 | Actin-related protein 2/3 complex subunit 1BAdd BLAST | 372 |
Proteomic databases
EPDi | Q9WV32 |
jPOSTi | Q9WV32 |
MaxQBi | Q9WV32 |
PaxDbi | Q9WV32 |
PRIDEi | Q9WV32 |
PTM databases
iPTMneti | Q9WV32 |
PhosphoSitePlusi | Q9WV32 |
SwissPalmi | Q9WV32 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029622, Expressed in humerus cartilage element and 175 other tissues |
Genevisiblei | Q9WV32, MM |
Interactioni
Subunit structurei
Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.
By similarityGO - Molecular functioni
- actin filament binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 198208, 20 interactors |
IntActi | Q9WV32, 4 interactors |
MINTi | Q9WV32 |
STRINGi | 10090.ENSMUSP00000082822 |
Miscellaneous databases
RNActi | Q9WV32, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 6 – 45 | WD 1Add BLAST | 40 | |
Repeati | 50 – 89 | WD 2Add BLAST | 40 | |
Repeati | 94 – 135 | WD 3Add BLAST | 42 | |
Repeati | 140 – 179 | WD 4Add BLAST | 40 | |
Repeati | 242 – 280 | WD 5Add BLAST | 39 | |
Repeati | 324 – 367 | WD 6Add BLAST | 44 |
Sequence similaritiesi
Belongs to the WD repeat ARPC1 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG1523, Eukaryota |
GeneTreei | ENSGT00950000183183 |
HOGENOMi | CLU_034396_1_0_1 |
InParanoidi | Q9WV32 |
OMAi | KWVQVHE |
OrthoDBi | 848569at2759 |
TreeFami | TF315041 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR030141, ARC1B IPR017383, ARPC1 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR10709, PTHR10709, 1 hit PTHR10709:SF10, PTHR10709:SF10, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 2 hits |
PIRSFi | PIRSF038093, ARP2/3_su1, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9WV32-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYHSFLVEP ISCHAWNKDR TQIAICPNNH EVHIYEKSGA KWNKVHELKE
60 70 80 90 100
HNGQVTGIDW APESNRIVTC GTDRNAYVWT LKGRTWKPTL VILRINRAAR
110 120 130 140 150
CVRWAPNENK FAVGSGSRVI SICYFEQEND WWVCKHIKKP IRSTVLSLDW
160 170 180 190 200
HPNNVLLAAG SCDFKCRIFS AYIKEVEERP APTPWGSKMP FGELMFESSS
210 220 230 240 250
SCGWVHGVCF SASGSRVAWV SHDSTVCLVD ADKKMAVATL ASETLPLLAV
260 270 280 290 300
TFITENSLVA AGHDCFPVLF TYDNAAVTLS FGGRLDVPKQ SSQRGMTARE
310 320 330 340 350
RFQNLDKKAS SEGGAATGAG LDSLHKNSVS QISVLSGGKA KCSQFCTTGM
360 370
DGGMSIWDVK SLESALKDLK IK
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ91Z25 | Q91Z25_MOUSE | Actin-related protein 2/3 complex s... | Arpc1b | 376 | Annotation score: | ||
F6VVE6 | F6VVE6_MOUSE | Actin-related protein 2/3 complex s... | Arpc1b | 232 | Annotation score: | ||
F6THG2 | F6THG2_MOUSE | Actin-related protein 2/3 complex s... | Arpc1b | 105 | Annotation score: | ||
D3Z6S0 | D3Z6S0_MOUSE | Actin-related protein 2/3 complex s... | Arpc1b | 56 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 | A → R in AAD43352 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF162768 mRNA Translation: AAD43352.1 AK002274 mRNA Translation: BAB21980.1 AK005378 mRNA Translation: BAB23985.1 AK147731 mRNA Translation: BAE28101.1 AK148145 mRNA Translation: BAE28373.1 AK159371 mRNA Translation: BAE35028.1 AK167978 mRNA Translation: BAE39970.1 AK168774 mRNA Translation: BAE40610.1 AK172124 mRNA Translation: BAE42835.1 CH466529 Genomic DNA Translation: EDL19001.1 CH466529 Genomic DNA Translation: EDL19002.1 BC003441 mRNA Translation: AAH03441.1 |
CCDSi | CCDS19856.1 |
RefSeqi | NP_075631.2, NM_023142.2 |
Genome annotation databases
Ensembli | ENSMUST00000085679; ENSMUSP00000082822; ENSMUSG00000029622 |
GeneIDi | 11867 |
KEGGi | mmu:11867 |
UCSCi | uc009amb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF162768 mRNA Translation: AAD43352.1 AK002274 mRNA Translation: BAB21980.1 AK005378 mRNA Translation: BAB23985.1 AK147731 mRNA Translation: BAE28101.1 AK148145 mRNA Translation: BAE28373.1 AK159371 mRNA Translation: BAE35028.1 AK167978 mRNA Translation: BAE39970.1 AK168774 mRNA Translation: BAE40610.1 AK172124 mRNA Translation: BAE42835.1 CH466529 Genomic DNA Translation: EDL19001.1 CH466529 Genomic DNA Translation: EDL19002.1 BC003441 mRNA Translation: AAH03441.1 |
CCDSi | CCDS19856.1 |
RefSeqi | NP_075631.2, NM_023142.2 |
3D structure databases
SMRi | Q9WV32 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198208, 20 interactors |
IntActi | Q9WV32, 4 interactors |
MINTi | Q9WV32 |
STRINGi | 10090.ENSMUSP00000082822 |
PTM databases
iPTMneti | Q9WV32 |
PhosphoSitePlusi | Q9WV32 |
SwissPalmi | Q9WV32 |
Proteomic databases
EPDi | Q9WV32 |
jPOSTi | Q9WV32 |
MaxQBi | Q9WV32 |
PaxDbi | Q9WV32 |
PRIDEi | Q9WV32 |
Protocols and materials databases
Antibodypediai | 1378, 202 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000085679; ENSMUSP00000082822; ENSMUSG00000029622 |
GeneIDi | 11867 |
KEGGi | mmu:11867 |
UCSCi | uc009amb.2, mouse |
Organism-specific databases
CTDi | 10095 |
MGIi | MGI:1343142, Arpc1b |
Phylogenomic databases
eggNOGi | KOG1523, Eukaryota |
GeneTreei | ENSGT00950000183183 |
HOGENOMi | CLU_034396_1_0_1 |
InParanoidi | Q9WV32 |
OMAi | KWVQVHE |
OrthoDBi | 848569at2759 |
TreeFami | TF315041 |
Enzyme and pathway databases
Reactomei | R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-3928662, EPHB-mediated forward signaling |
Miscellaneous databases
BioGRID-ORCSi | 11867, 0 hits in 17 CRISPR screens |
ChiTaRSi | Arpc1b, mouse |
PROi | PR:Q9WV32 |
RNActi | Q9WV32, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029622, Expressed in humerus cartilage element and 175 other tissues |
Genevisiblei | Q9WV32, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR030141, ARC1B IPR017383, ARPC1 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR10709, PTHR10709, 1 hit PTHR10709:SF10, PTHR10709:SF10, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 2 hits |
PIRSFi | PIRSF038093, ARP2/3_su1, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARC1B_MOUSE | |
Accessioni | Q9WV32Primary (citable) accession number: Q9WV32 Secondary accession number(s): Q9CRC4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families