UniProtKB - Q9WV27 (AT1A4_MOUSE)
Protein
Sodium/potassium-transporting ATPase subunit alpha-4
Gene
Atp1a4
Organism
Mus musculus (Mouse)
Status
Functioni
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility (By similarity).By similarity
Catalytic activityi
- EC:7.2.2.13
Activity regulationi
Specifically inhibited by an endogenous cardiac glycoside, ouabain.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 386 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 726 | MagnesiumBy similarity | 1 | |
Metal bindingi | 730 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled cation transmembrane transporter activity Source: MGI
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- sodium:potassium-exchanging ATPase activity Source: MGI
GO - Biological processi
- cellular potassium ion homeostasis Source: GO_Central
- cellular sodium ion homeostasis Source: GO_Central
- fertilization Source: MGI
- flagellated sperm motility Source: MGI
- potassium ion import across plasma membrane Source: GO_Central
- potassium ion transmembrane transport Source: MGI
- proton transmembrane transport Source: GO_Central
- regulation of cellular pH Source: UniProtKB
- regulation of membrane potential Source: MGI
- sodium ion export across plasma membrane Source: GO_Central
- sodium ion transmembrane transport Source: MGI
- sodium ion transport Source: MGI
- spermatogenesis Source: MGI
Keywordsi
Molecular function | Translocase |
Biological process | Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium |
Enzyme and pathway databases
Reactomei | R-MMU-5578775, Ion homeostasis R-MMU-936837, Ion transport by P-type ATPases |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/potassium-transporting ATPase subunit alpha-4 (EC:7.2.2.13)Short name: Na(+)/K(+) ATPase alpha-4 subunit Alternative name(s): Sodium pump subunit alpha-4 |
Gene namesi | Name:Atp1a4 Synonyms:Atp1al2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351335, Atp1a4 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- sodium:potassium-exchanging ATPase complex Source: MGI
Other locations
- photoreceptor cell cilium Source: MGI
- rod photoreceptor outer segment Source: MGI
- sperm midpiece Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 96 | CytoplasmicSequence analysisAdd BLAST | 96 | |
Transmembranei | 97 – 117 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 118 – 141 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 142 – 162 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 163 – 298 | CytoplasmicSequence analysisAdd BLAST | 136 | |
Transmembranei | 299 – 318 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 319 – 330 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 331 – 348 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 349 – 781 | CytoplasmicSequence analysisAdd BLAST | 433 | |
Transmembranei | 782 – 801 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 802 – 811 | ExtracellularSequence analysis | 10 | |
Transmembranei | 812 – 832 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 833 – 852 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 853 – 875 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 876 – 927 | ExtracellularSequence analysisAdd BLAST | 52 | |
Transmembranei | 928 – 947 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 948 – 960 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 961 – 979 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 980 – 994 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 995 – 1015 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1016 – 1032 | CytoplasmicSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046304 | 1 – 1032 | Sodium/potassium-transporting ATPase subunit alpha-4Add BLAST | 1032 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 952 | Phosphoserine; by PKABy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9WV27 |
jPOSTi | Q9WV27 |
MaxQBi | Q9WV27 |
PaxDbi | Q9WV27 |
PeptideAtlasi | Q9WV27 |
PRIDEi | Q9WV27 |
PTM databases
iPTMneti | Q9WV27 |
PhosphoSitePlusi | Q9WV27 |
Expressioni
Tissue specificityi
Expressed at high levels in the testis and at low levels in the epididymis.
Gene expression databases
Bgeei | ENSMUSG00000007107, Expressed in spermatid and 71 other tissues |
Genevisiblei | Q9WV27, MM |
Interactioni
Subunit structurei
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
CuratedProtein-protein interaction databases
IntActi | Q9WV27, 5 interactors |
MINTi | Q9WV27 |
STRINGi | 10090.ENSMUSP00000106874 |
Miscellaneous databases
RNActi | Q9WV27, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 91 – 93 | Interaction with phosphoinositide-3 kinaseBy similarity | 3 |
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0203, Eukaryota |
GeneTreei | ENSGT00940000162378 |
HOGENOMi | CLU_002360_3_3_1 |
InParanoidi | Q9WV27 |
OMAi | FAWDKPE |
OrthoDBi | 388324at2759 |
TreeFami | TF312838 |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9WV27-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPGKEKEVE APGELNQKPR PSTRSSTTNR QPKMKRRKKD LEELKKEVVM
60 70 80 90 100
DDHKLTLDEL SAKYSVDLTK GLSILEAQDI LFQNGPNVLT PPPTTPEWVK
110 120 130 140 150
FCRQLFGGFS LLLWTGACLC FLAYGIHVNY YKENANKDNL YLGIVLSAVV
160 170 180 190 200
IITGCFSYYQ EAKSSKIMES FKNMVPQQAL VIRDGEKMQI NVRDVVLGDL
210 220 230 240 250
VEVKGGDQIP ADIRVISAQG CKVDNSSLTG ESEPQSRCPD CTHENPLETR
260 270 280 290 300
NIIFFSTNCV EGTARGIVIA TGDYTVMGRI ASLTSGLQMG KTPIATEIEH
310 320 330 340 350
FIHLITAVAV FLGVSFFWLS IILGYTWLDA VIFLIGIIVA NVPEGLLATV
360 370 380 390 400
TVCLTLTAKR MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH
410 420 430 440 450
LWFDKTVYEA DTSEEQTTGK TFPKSSDTWF YLARIAGLCN RADFKPHQES
460 470 480 490 500
VPIAKRATTG DASESALLKF IEQSYNPVSE MRQKNPKVAE IPFNSTNKYQ
510 520 530 540 550
MSIHLLEDNS EAHVLLMKGA PERIFDFCSS FLLNGQEYPM DEEMKMDFQN
560 570 580 590 600
AYIELGGLGE RVLGFCFLNL PSNFSKGFQF NTDELNFPME NLCFAGLISM
610 620 630 640 650
IDPPRTAVPD AVSKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISEGNDTA
660 670 680 690 700
EDIAARLNIP ISQVNNKSVK AIVVHGSELK DMESQQLDDI LKSYKEIVFA
710 720 730 740 750
RTSPQQKLII VEGCQRLGAI VAVTGDGVND SPALKKADIG IAMGITGSDV
760 770 780 790 800
SKQAADMILL DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEITPFL
810 820 830 840 850
LFIILSIPLP LGTITILCID LGTDMVPAIS LAYESPESDI MKRLPRNPKT
860 870 880 890 900
DNLVNNRLIG MAYGQIGMIQ ALAGFFTYFV ILAENGFKPL DLLGIRLYWD
910 920 930 940 950
DTQLNDLEDS YGQQWTYEQR KVVEFTCQTA FFISIVIVQW ADLIICKTRR
960 970 980 990 1000
NSLFKQGMKN KILIFGLLEE TVLAAFLSYV PGMDVSLRMY PLKINWWFCA
1010 1020 1030
LPYSVLIFVY DEIRKLIIRR RPGGWLEKET YY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | I → V in AAD43812 (PubMed:10555956).Curated | 1 | |
Sequence conflicti | 476 | N → S in AAD43813 (PubMed:10555956).Curated | 1 | |
Sequence conflicti | 546 | M → T in AAD43813 (PubMed:10555956).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF164348 Genomic DNA Translation: AAD43812.1 AF164349 Genomic DNA Translation: AAD43813.1 AF164350 Genomic DNA Translation: AAD43814.1 AC074310 Genomic DNA No translation available. AC087061 Genomic DNA No translation available. |
CCDSi | CCDS48446.1 |
RefSeqi | NP_038762.1, NM_013734.1 |
Genome annotation databases
Ensembli | ENSMUST00000111243; ENSMUSP00000106874; ENSMUSG00000007107 |
GeneIDi | 27222 |
KEGGi | mmu:27222 |
UCSCi | uc011wwk.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF164348 Genomic DNA Translation: AAD43812.1 AF164349 Genomic DNA Translation: AAD43813.1 AF164350 Genomic DNA Translation: AAD43814.1 AC074310 Genomic DNA No translation available. AC087061 Genomic DNA No translation available. |
CCDSi | CCDS48446.1 |
RefSeqi | NP_038762.1, NM_013734.1 |
3D structure databases
SMRi | Q9WV27 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9WV27, 5 interactors |
MINTi | Q9WV27 |
STRINGi | 10090.ENSMUSP00000106874 |
PTM databases
iPTMneti | Q9WV27 |
PhosphoSitePlusi | Q9WV27 |
Proteomic databases
EPDi | Q9WV27 |
jPOSTi | Q9WV27 |
MaxQBi | Q9WV27 |
PaxDbi | Q9WV27 |
PeptideAtlasi | Q9WV27 |
PRIDEi | Q9WV27 |
Protocols and materials databases
Antibodypediai | 55096, 12 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000111243; ENSMUSP00000106874; ENSMUSG00000007107 |
GeneIDi | 27222 |
KEGGi | mmu:27222 |
UCSCi | uc011wwk.1, mouse |
Organism-specific databases
CTDi | 480 |
MGIi | MGI:1351335, Atp1a4 |
Phylogenomic databases
eggNOGi | KOG0203, Eukaryota |
GeneTreei | ENSGT00940000162378 |
HOGENOMi | CLU_002360_3_3_1 |
InParanoidi | Q9WV27 |
OMAi | FAWDKPE |
OrthoDBi | 388324at2759 |
TreeFami | TF312838 |
Enzyme and pathway databases
Reactomei | R-MMU-5578775, Ion homeostasis R-MMU-936837, Ion transport by P-type ATPases |
Miscellaneous databases
BioGRID-ORCSi | 27222, 0 hits in 17 CRISPR screens |
PROi | PR:Q9WV27 |
RNActi | Q9WV27, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000007107, Expressed in spermatid and 71 other tissues |
Genevisiblei | Q9WV27, MM |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AT1A4_MOUSE | |
Accessioni | Q9WV27Primary (citable) accession number: Q9WV27 Secondary accession number(s): E9QKL5, Q9R173, Q9WV28 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families