UniProtKB - Q9WUR2 (ECI2_MOUSE)
Protein
Enoyl-CoA delta isomerase 2, mitochondrial
Gene
Eci2
Organism
Mus musculus (Mouse)
Status
Functioni
Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (PubMed:24344334). Has a preference for 3-trans substrates (By similarity).By similarity1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 90 | Acyl-CoABy similarity | 1 | |
Binding sitei | 109 | Acyl-CoABy similarity | 1 | |
Sitei | 277 | Important for catalytic activityBy similarity | 1 |
GO - Molecular functioni
- dodecenoyl-CoA delta-isomerase activity Source: MGI
- fatty-acyl-CoA binding Source: InterPro
- intramolecular oxidoreductase activity, transposing C=C bonds Source: MGI
GO - Biological processi
- fatty acid beta-oxidation Source: MGI
- fatty acid catabolic process Source: UniProtKB
Keywordsi
Molecular function | Isomerase |
Enzyme and pathway databases
Reactomei | R-MMU-390247 Beta-oxidation of very long chain fatty acids R-MMU-9033241 Peroxisomal protein import |
Chemistry databases
SwissLipidsi | SLP:000001194 |
Names & Taxonomyi
Protein namesi | Recommended name: Enoyl-CoA delta isomerase 2, mitochondrial (EC:5.3.3.81 Publication)Alternative name(s): Delta(3),delta(2)-enoyl-CoA isomerase Short name: D3,D2-enoyl-CoA isomerase Dodecenoyl-CoA isomerase Peroxisomal 3,2-trans-enoyl-CoA isomerase Short name: pECI |
Gene namesi | Name:Eci2 Synonyms:Peci |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346064 Eci2 |
Subcellular locationi
Isoform 1 :
Mitochondrion
- Mitochondrion 1 Publication
Isoform 2 :
Peroxisome
- Peroxisome matrix 1 Publication
Mitochondrion
- mitochondrion Source: MGI
Peroxisome
- peroxisomal matrix Source: UniProtKB
- peroxisome Source: HGNC-UCL
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Mitochondrion, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 36 | MitochondrionSequence analysisAdd BLAST | 36 | |
ChainiPRO_0000214028 | 37 – 391 | Enoyl-CoA delta isomerase 2, mitochondrialAdd BLAST | 355 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 49 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 49 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 53 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 60 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 60 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 68 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 79 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 88 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 90 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 90 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 99 | PhosphoserineBy similarity | 1 | |
Modified residuei | 117 | PhosphoserineBy similarity | 1 | |
Modified residuei | 127 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 159 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 286 | N6-succinyllysineCombined sources | 1 |
Post-translational modificationi
Acetylation of Lys-60 is observed in liver mitochondria from fasted mice but not from fed mice.
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q9WUR2 |
PaxDbi | Q9WUR2 |
PeptideAtlasi | Q9WUR2 |
PRIDEi | Q9WUR2 |
PTM databases
iPTMneti | Q9WUR2 |
PhosphoSitePlusi | Q9WUR2 |
SwissPalmi | Q9WUR2 |
Expressioni
Tissue specificityi
Expressed in liver and kidney (at protein level).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000021417 Expressed in 312 organ(s), highest expression level in saccule of membranous labyrinth |
ExpressionAtlasi | Q9WUR2 baseline and differential |
Genevisiblei | Q9WUR2 MM |
Interactioni
Protein-protein interaction databases
IntActi | Q9WUR2, 4 interactors |
STRINGi | 10090.ENSMUSP00000131735 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 122 | ACBPROSITE-ProRule annotationAdd BLAST | 86 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 68 | Acyl-CoA bindingBy similarity | 5 | |
Regioni | 149 – 319 | ECH-likeAdd BLAST | 171 | |
Regioni | 196 – 200 | Substrate bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 389 – 391 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0016 Eukaryota KOG0817 Eukaryota COG1024 LUCA COG4281 LUCA |
GeneTreei | ENSGT00940000155105 |
InParanoidi | Q9WUR2 |
KOi | K13239 |
OMAi | LGYHRAA |
OrthoDBi | 1471901at2759 |
PhylomeDBi | Q9WUR2 |
TreeFami | TF313375 |
Family and domain databases
CDDi | cd00435 ACBP, 1 hit |
Gene3Di | 1.10.12.10, 1 hit 1.20.80.10, 1 hit |
InterProi | View protein in InterPro IPR022408 Acyl-CoA-binding_prot_CS IPR000582 Acyl-CoA-binding_protein IPR035984 Acyl-CoA-binding_sf IPR029045 ClpP/crotonase-like_dom_sf IPR001753 Enoyl-CoA_hydra/iso IPR014748 Enoyl-CoA_hydra_C IPR014352 FERM/acyl-CoA-bd_prot_sf |
Pfami | View protein in Pfam PF00887 ACBP, 1 hit PF00378 ECH_1, 1 hit |
PRINTSi | PR00689 ACOABINDINGP |
SUPFAMi | SSF47027 SSF47027, 1 hit SSF52096 SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00880 ACB_1, 1 hit PS51228 ACB_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9WUR2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAVTWSRAR CWCPSVLQVF RLQVAKLHLG RPTMRASQQD FENALNQVKL
60 70 80 90 100
LKKDPGNEVK LRLYALYKQA TEGPCNMPKP GMLDFVNKAK WDAWNALGSL
110 120 130 140 150
PKETARQNYV DLVSSLSSSS EAPSQGKRGA DEKARESKDI LVTSEDGITK
160 170 180 190 200
ITFNRPTKKN AISFQMYRDI ILALKNASTD NTVMAVFTGT GDYYCSGNDL
210 220 230 240 250
TNFTSATGGI EEAASNGAVL LRDFVNSFID FPKPLVAVVN GPAVGISVTL
260 270 280 290 300
LGLFDAVFAS DRATFHTPFS QLGQSPEACS SYTFPKMMGS AKAAEMLLFG
310 320 330 340 350
KKLTAREAWA QGLVTEVFPE STFETEVWTR LKTYAKLPPN AMRISKELIR
360 370 380 390
KNEKEKLYAV NAEECTTLQA RWLSEECMNA IMSFVSRKPK L
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TCD4 | Q3TCD4_MOUSE | Enoyl-CoA delta isomerase 2, mitoch... | Eci2 Peci | 378 | Annotation score: | ||
E9Q7A8 | E9Q7A8_MOUSE | Enoyl-CoA delta isomerase 2, mitoch... | Eci2 | 119 | Annotation score: | ||
E9PUY9 | E9PUY9_MOUSE | Enoyl-CoA delta isomerase 2, mitoch... | Eci2 | 202 | Annotation score: | ||
E9PVM6 | E9PVM6_MOUSE | Enoyl-CoA delta isomerase 2, mitoch... | Eci2 | 229 | Annotation score: | ||
E9PYA9 | E9PYA9_MOUSE | Enoyl-CoA delta isomerase 2, mitoch... | Eci2 | 216 | Annotation score: | ||
E9PYC6 | E9PYC6_MOUSE | Enoyl-CoA delta isomerase 2, mitoch... | Eci2 | 170 | Annotation score: | ||
E9Q858 | E9Q858_MOUSE | Enoyl-CoA delta isomerase 2, mitoch... | Eci2 | 199 | Annotation score: |
Sequence cautioni
The sequence AAH01983 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 | R → K in BAB26315 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 128 | R → C in AAH01983 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 135 | R → Q in AAH01983 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037855 | 1 – 33 | Missing in isoform 2. 1 PublicationAdd BLAST | 33 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF153613 mRNA Translation: AAD34174.1 AK009478 mRNA Translation: BAB26315.2 BC001983 mRNA Translation: AAH01983.1 Different initiation. |
CCDSi | CCDS26450.1 [Q9WUR2-2] CCDS49234.1 [Q9WUR2-1] |
RefSeqi | NP_001103801.1, NM_001110331.1 [Q9WUR2-1] NP_035998.2, NM_011868.3 [Q9WUR2-2] XP_006516736.1, XM_006516673.2 [Q9WUR2-2] XP_006516737.1, XM_006516674.2 [Q9WUR2-2] XP_006516738.1, XM_006516675.2 [Q9WUR2-2] XP_006516739.1, XM_006516676.3 [Q9WUR2-2] XP_006516740.1, XM_006516677.1 [Q9WUR2-2] |
Genome annotation databases
Ensembli | ENSMUST00000021854; ENSMUSP00000021854; ENSMUSG00000021417 [Q9WUR2-2] ENSMUST00000171229; ENSMUSP00000131735; ENSMUSG00000021417 [Q9WUR2-1] ENSMUST00000178421; ENSMUSP00000137411; ENSMUSG00000021417 [Q9WUR2-2] |
GeneIDi | 23986 |
KEGGi | mmu:23986 |
UCSCi | uc007qbx.3 mouse [Q9WUR2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF153613 mRNA Translation: AAD34174.1 AK009478 mRNA Translation: BAB26315.2 BC001983 mRNA Translation: AAH01983.1 Different initiation. |
CCDSi | CCDS26450.1 [Q9WUR2-2] CCDS49234.1 [Q9WUR2-1] |
RefSeqi | NP_001103801.1, NM_001110331.1 [Q9WUR2-1] NP_035998.2, NM_011868.3 [Q9WUR2-2] XP_006516736.1, XM_006516673.2 [Q9WUR2-2] XP_006516737.1, XM_006516674.2 [Q9WUR2-2] XP_006516738.1, XM_006516675.2 [Q9WUR2-2] XP_006516739.1, XM_006516676.3 [Q9WUR2-2] XP_006516740.1, XM_006516677.1 [Q9WUR2-2] |
3D structure databases
SMRi | Q9WUR2 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9WUR2, 4 interactors |
STRINGi | 10090.ENSMUSP00000131735 |
Chemistry databases
SwissLipidsi | SLP:000001194 |
PTM databases
iPTMneti | Q9WUR2 |
PhosphoSitePlusi | Q9WUR2 |
SwissPalmi | Q9WUR2 |
Proteomic databases
jPOSTi | Q9WUR2 |
PaxDbi | Q9WUR2 |
PeptideAtlasi | Q9WUR2 |
PRIDEi | Q9WUR2 |
Genome annotation databases
Ensembli | ENSMUST00000021854; ENSMUSP00000021854; ENSMUSG00000021417 [Q9WUR2-2] ENSMUST00000171229; ENSMUSP00000131735; ENSMUSG00000021417 [Q9WUR2-1] ENSMUST00000178421; ENSMUSP00000137411; ENSMUSG00000021417 [Q9WUR2-2] |
GeneIDi | 23986 |
KEGGi | mmu:23986 |
UCSCi | uc007qbx.3 mouse [Q9WUR2-1] |
Organism-specific databases
CTDi | 10455 |
MGIi | MGI:1346064 Eci2 |
Phylogenomic databases
eggNOGi | KOG0016 Eukaryota KOG0817 Eukaryota COG1024 LUCA COG4281 LUCA |
GeneTreei | ENSGT00940000155105 |
InParanoidi | Q9WUR2 |
KOi | K13239 |
OMAi | LGYHRAA |
OrthoDBi | 1471901at2759 |
PhylomeDBi | Q9WUR2 |
TreeFami | TF313375 |
Enzyme and pathway databases
Reactomei | R-MMU-390247 Beta-oxidation of very long chain fatty acids R-MMU-9033241 Peroxisomal protein import |
Miscellaneous databases
ChiTaRSi | Eci2 mouse |
PROi | PR:Q9WUR2 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021417 Expressed in 312 organ(s), highest expression level in saccule of membranous labyrinth |
ExpressionAtlasi | Q9WUR2 baseline and differential |
Genevisiblei | Q9WUR2 MM |
Family and domain databases
CDDi | cd00435 ACBP, 1 hit |
Gene3Di | 1.10.12.10, 1 hit 1.20.80.10, 1 hit |
InterProi | View protein in InterPro IPR022408 Acyl-CoA-binding_prot_CS IPR000582 Acyl-CoA-binding_protein IPR035984 Acyl-CoA-binding_sf IPR029045 ClpP/crotonase-like_dom_sf IPR001753 Enoyl-CoA_hydra/iso IPR014748 Enoyl-CoA_hydra_C IPR014352 FERM/acyl-CoA-bd_prot_sf |
Pfami | View protein in Pfam PF00887 ACBP, 1 hit PF00378 ECH_1, 1 hit |
PRINTSi | PR00689 ACOABINDINGP |
SUPFAMi | SSF47027 SSF47027, 1 hit SSF52096 SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00880 ACB_1, 1 hit PS51228 ACB_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ECI2_MOUSE | |
Accessioni | Q9WUR2Primary (citable) accession number: Q9WUR2 Secondary accession number(s): Q99M61, Q9D785 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | September 1, 2009 | |
Last modified: | November 13, 2019 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot