UniProtKB - Q9WUQ1 (ATS1_RAT)
Protein
A disintegrin and metalloproteinase with thrombospondin motifs 1
Gene
Adamts1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Cleaves aggrecan, a cartilage proteoglycan, at the '1683-Glu-|-Leu-1684' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity). Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity).By similarity
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 205 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 261 | Calcium 1By similarity | 1 | |
Metal bindingi | 261 | Calcium 2By similarity | 1 | |
Metal bindingi | 344 | Calcium 1By similarity | 1 | |
Metal bindingi | 344 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 351 | Calcium 1By similarity | 1 | |
Metal bindingi | 401 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 402 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 405 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 411 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 462 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 465 | Calcium 1By similarity | 1 | |
Metal bindingi | 465 | Calcium 2By similarity | 1 |
GO - Molecular functioni
- heparin binding Source: RGD
- metalloendopeptidase activity Source: RGD
- zinc ion binding Source: InterPro
GO - Biological processi
- cellular response to parathyroid hormone stimulus Source: RGD
- cellular response to prostaglandin E stimulus Source: RGD
- cellular response to vitamin D Source: RGD
- extracellular matrix organization Source: GO_Central
- heart trabecula formation Source: RGD
- kidney development Source: RGD
- negative regulation of angiogenesis Source: RGD
- ovulation Source: RGD
- ovulation from ovarian follicle Source: RGD
- positive regulation of G1/S transition of mitotic cell cycle Source: RGD
- positive regulation of neuron projection development Source: RGD
- positive regulation of vascular associated smooth muscle cell migration Source: RGD
- positive regulation of vascular associated smooth muscle cell proliferation Source: RGD
- tooth eruption Source: RGD
Keywordsi
Molecular function | Heparin-binding, Hydrolase, Metalloprotease, Protease |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.24.B11, 5301 |
Reactomei | R-RNO-5173214, O-glycosylation of TSR domain-containing proteins |
Protein family/group databases
MEROPSi | M12.222 |
Names & Taxonomyi
Protein namesi | Recommended name: A disintegrin and metalloproteinase with thrombospondin motifs 1 (EC:3.4.24.-)Short name: ADAM-TS 1 Short name: ADAM-TS1 Short name: ADAMTS-1 |
Gene namesi | Name:Adamts1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621241, Adamts1 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
Extracellular region or secreted
- basement membrane Source: RGD
- collagen-containing extracellular matrix Source: RGD
- extracellular matrix Source: RGD
Other locations
- cytoplasmic vesicle Source: RGD
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 54 | Sequence analysisAdd BLAST | 54 | |
PropeptideiPRO_0000029154 | 55 – 252 | By similarityAdd BLAST | 198 | |
ChainiPRO_0000029155 | 253 – 967 | A disintegrin and metalloproteinase with thrombospondin motifs 1Add BLAST | 715 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 333 ↔ 385 | By similarity | ||
Disulfide bondi | 362 ↔ 367 | By similarity | ||
Disulfide bondi | 379 ↔ 462 | By similarity | ||
Disulfide bondi | 417 ↔ 446 | By similarity | ||
Disulfide bondi | 488 ↔ 511 | By similarity | ||
Disulfide bondi | 499 ↔ 521 | By similarity | ||
Disulfide bondi | 506 ↔ 540 | By similarity | ||
Disulfide bondi | 534 ↔ 545 | By similarity | ||
Glycosylationi | 547 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 571 ↔ 608 | By similarity | ||
Disulfide bondi | 575 ↔ 613 | By similarity | ||
Disulfide bondi | 586 ↔ 598 | By similarity | ||
Glycosylationi | 720 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 764 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 782 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 945 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | Q9WUQ1 |
PRIDEi | Q9WUQ1 |
PTM databases
GlyGeni | Q9WUQ1, 5 sites |
Expressioni
Inductioni
Down-regulated in endothelial cells derived from cirrhotic liver.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 258 – 467 | Peptidase M12BPROSITE-ProRule annotationAdd BLAST | 210 | |
Domaini | 476 – 558 | DisintegrinAdd BLAST | 83 | |
Domaini | 559 – 614 | TSP type-1 1PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 854 – 910 | TSP type-1 2PROSITE-ProRule annotationAdd BLAST | 57 | |
Domaini | 911 – 967 | TSP type-1 3PROSITE-ProRule annotationAdd BLAST | 57 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 725 – 857 | SpacerAdd BLAST | 133 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 203 – 210 | Cysteine switchBy similarity | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 194 – 198 | Poly-Arg | 5 | |
Compositional biasi | 616 – 724 | Cys-richAdd BLAST | 109 |
Domaini
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG3538, Eukaryota |
InParanoidi | Q9WUQ1 |
PhylomeDBi | Q9WUQ1 |
Family and domain databases
Gene3Di | 2.20.100.10, 3 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR041645, ADAM_CR_2 IPR006586, ADAM_Cys-rich IPR010294, ADAM_spacer1 IPR013273, ADAMTS/ADAMTS-like IPR024079, MetalloPept_cat_dom_sf IPR013274, Pept_M12B_ADAM-TS1 IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01858, ADAMTS1 PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00209, TSP1, 3 hits |
SUPFAMi | SSF82895, SSF82895, 3 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50092, TSP1, 3 hits PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9WUQ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQPEVPLGSG KLKPCSDMGD IQRAAKFRSS QSAHMLLLLL ASITMLLCVR
60 70 80 90 100
GAHGRPTEED EELVLPSLER ARGHDSTTLL RLDAFGQQLH LKLQPDSGFL
110 120 130 140 150
APGFTLQTVG RSPGSEAQHL DPTGDLAHCF YSGTVNGDPS SAAALSLCEG
160 170 180 190 200
VRGAFYLQGE EFFIQPAPAV ATERLVPAEP KEESIAPPRF HILRRRRRGS
210 220 230 240 250
GGAKCGVMDE ETLPTSNSGR ESQNTPDQWP LRNPTPQGAG KPTGPGSIRK
260 270 280 290 300
KRFVSSPRYV ETMLVADQSM ADFHGSGLKH YLLTLFSVAA RFYKHPSIRN
310 320 330 340 350
SISLVVVKIL VIYEEQKGPE VTSNAALTLR NFCSWQKQHN SPSDRDPEHY
360 370 380 390 400
DTAILFTRQD LCGSHTCDTL GMADVGTVCD PSRSCSVIED DGLQAAFTTA
410 420 430 440 450
HELGHVFNMP HDDAKHCASF NGVSGDSHLM ASMLSSLDHS QPWSPCSAYM
460 470 480 490 500
VTSFLDNGHG ECLMDKPQNP IKLPSDLPGT LYDANRQCQF TFGEESTHCP
510 520 530 540 550
DAASTCSTLW CTGTSGGLLV CQTKHFPWAD GTSCGEGKWC VSGKCVNKTD
560 570 580 590 600
MKHFATPVHG SWGPWGPWGD CSRTCGGGVQ YTMRECDNPV PKNGGKYCEG
610 620 630 640 650
KRVRYRSCNI EDCPDNNGKT FREEQCEAHN EFSKASFGNE PTVEWTPKYA
660 670 680 690 700
GVSPKDRCKL TCEAKGIGYF FVLQPKVVDG TPCSPDSTSV CVQGQCVKAG
710 720 730 740 750
CDRIIDSKKK FDKCGVCGGN GSTCKKISGT VTSTRPGYHD IVTIPAGATN
760 770 780 790 800
IEVKHRNPRG SRNNGSFLAI RAADGTYILN GNFTLSTLEQ DLTYKGTVLR
810 820 830 840 850
YSGSSAALER IRSFSPLKEP LTIQVLMVGH ALRPKIKYTY FMKKKTEPFN
860 870 880 890 900
AIPTFSEWVI EEWGECSKTC GSGWQRRVVE CRDINGHPAS ECAKEVKPAS
910 920 930 940 950
TRPCADLPCP RWQVGDWSPC SKTCGKGYKK RTLKCLSHDG GVLSNESCDP
960
LKKPKHYIDF CILTQCS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ68EJ2 | Q68EJ2_RAT | A disintegrin and metalloproteinase... | Adamts1 rCG_58766 | 967 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 21 | I → V in AAG29823 (PubMed:10847486).Curated | 1 | |
Sequence conflicti | 26 – 31 | KFRSSQ → RSRGSL in AAG29823 (PubMed:10847486).Curated | 6 | |
Sequence conflicti | 49 | V → A in AAG29823 (PubMed:10847486).Curated | 1 | |
Sequence conflicti | 72 | R → P in AAG29823 (PubMed:10847486).Curated | 1 | |
Sequence conflicti | 79 | L → TR in AAG29823 (PubMed:10847486).Curated | 1 | |
Sequence conflicti | 249 | R → G in AAG29823 (PubMed:10847486).Curated | 1 | |
Sequence conflicti | 262 – 265 | TMLV → NLLK in AAG29823 (PubMed:10847486).Curated | 4 | |
Sequence conflicti | 607 | S → F in AAG29823 (PubMed:10847486).Curated | 1 | |
Sequence conflicti | 936 | L → V in AAG29823 (PubMed:10847486).Curated | 1 | |
Sequence conflicti | 962 | I → T in AAG29823 (PubMed:10847486).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF149118 mRNA Translation: AAD34012.1 AF304446 mRNA Translation: AAG29823.1 |
Genome annotation databases
UCSCi | RGD:621241, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF149118 mRNA Translation: AAD34012.1 AF304446 mRNA Translation: AAG29823.1 |
3D structure databases
SMRi | Q9WUQ1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000002187 |
Protein family/group databases
MEROPSi | M12.222 |
PTM databases
GlyGeni | Q9WUQ1, 5 sites |
Proteomic databases
PaxDbi | Q9WUQ1 |
PRIDEi | Q9WUQ1 |
Genome annotation databases
UCSCi | RGD:621241, rat |
Organism-specific databases
RGDi | 621241, Adamts1 |
Phylogenomic databases
eggNOGi | KOG3538, Eukaryota |
InParanoidi | Q9WUQ1 |
PhylomeDBi | Q9WUQ1 |
Enzyme and pathway databases
BRENDAi | 3.4.24.B11, 5301 |
Reactomei | R-RNO-5173214, O-glycosylation of TSR domain-containing proteins |
Miscellaneous databases
PROi | PR:Q9WUQ1 |
Family and domain databases
Gene3Di | 2.20.100.10, 3 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR041645, ADAM_CR_2 IPR006586, ADAM_Cys-rich IPR010294, ADAM_spacer1 IPR013273, ADAMTS/ADAMTS-like IPR024079, MetalloPept_cat_dom_sf IPR013274, Pept_M12B_ADAM-TS1 IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01858, ADAMTS1 PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00209, TSP1, 3 hits |
SUPFAMi | SSF82895, SSF82895, 3 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50092, TSP1, 3 hits PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATS1_RAT | |
Accessioni | Q9WUQ1Primary (citable) accession number: Q9WUQ1 Secondary accession number(s): Q9ERI1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1999 | |
Last modified: | October 7, 2020 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |