UniProtKB - Q9WUM4 (COR1C_MOUSE)
Protein
Coronin-1C
Gene
Coro1c
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in directed cell migration by regulating the activation and subcellular location of RAC1 (PubMed:25074804, PubMed:25925950). Increases the presence of activated RAC1 at the leading edge of migrating cells (PubMed:25074804, PubMed:25925950). Required for normal organization of the cytoskeleton, including the actin cytoskeleton, microtubules and the vimentin intermediate filaments (PubMed:27178841). Required for normal cell proliferation, cell migration, and normal formation of lamellipodia (PubMed:27178841). Plays a role in endoplasmic reticulum-associated endosome fission: localizes to endosome membrane tubules and promotes recruitment of TMCC1, leading to recruitment of the endoplasmic reticulum to endosome tubules for fission. Endosome membrane fission of early and late endosomes is essential to separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane (By similarity). Required for normal distribution of mitochondria within cells (PubMed:27178841).By similarity3 Publications
GO - Molecular functioni
- actin filament binding Source: MGI
- Rac GTPase binding Source: UniProtKB
GO - Biological processi
- actin cortical patch assembly Source: MGI
- actin cytoskeleton organization Source: MGI
- actin filament organization Source: GO_Central
- activation of GTPase activity Source: UniProtKB
- cell migration Source: GO_Central
- endosomal transport Source: UniProtKB
- endosome fission Source: UniProtKB
- endosome membrane tubulation Source: UniProtKB
- establishment of protein localization Source: UniProtKB
- membrane fission Source: UniProtKB
- negative regulation of epithelial cell migration Source: MGI
- negative regulation of focal adhesion assembly Source: MGI
- negative regulation of protein kinase activity by regulation of protein phosphorylation Source: MGI
- negative regulation of protein phosphorylation Source: MGI
- negative regulation of substrate adhesion-dependent cell spreading Source: MGI
- neural crest cell migration Source: MGI
- phagocytosis Source: MGI
- positive regulation of lamellipodium morphogenesis Source: MGI
- regulation of epithelial cell migration Source: MGI
- regulation of fibroblast migration Source: UniProtKB
- regulation of focal adhesion assembly Source: MGI
- regulation of protein phosphorylation Source: MGI
- regulation of ruffle assembly Source: UniProtKB
- regulation of substrate adhesion-dependent cell spreading Source: MGI
Keywordsi
Molecular function | Actin-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Coronin-1CAlternative name(s): Coronin-31 Publication |
Gene namesi | Name:Coro1c |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1345964, Coro1c |
Subcellular locationi
Cytoskeleton
- cytoskeleton 4 Publications
Plasma membrane
- Cell membrane 4 Publications; Peripheral membrane protein 4 Publications; Cytoplasmic side 4 Publications
- ruffle membrane 1 Publication
Endosome
- Endosome membrane By similarity
Other locations
- lamellipodium 2 Publications
- cell cortex 2 Publications
Note: Colocalizes with the actin cytoskeleton in the cytosol, and especially in the cell cortex (PubMed:19651142, PubMed:22364218, PubMed:25074804, PubMed:27178841). Colocalizes with F-actin at the leading edge of lamellipodia (PubMed:22364218). Partially colocalizes with microtubules and vimentin intermediate filaments (PubMed:27178841). Localizes to endosome membrane tubules/buds (By similarity).By similarity4 Publications
Cytoskeleton
- actin cytoskeleton Source: MGI
- cytoskeleton Source: UniProtKB
Endosome
- endosome membrane Source: UniProtKB
Plasma Membrane
- flotillin complex Source: UniProtKB
- lateral plasma membrane Source: MGI
- ruffle membrane Source: UniProtKB-SubCell
Other locations
- cell cortex Source: UniProtKB-SubCell
- lamellipodium Source: MGI
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Endosome, MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype (PubMed:27178841). Fibroblasts from mutant mice display a disordered actin cytoskeleton with a reduced width of the actin stress fibers. Likewise, these cells have several microtubule-organizing centers (MTOCs) and a disordered microbutule network (PubMed:27178841).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 28 | R → D: Decreased actin-binding. Loss of actin binding; when associated with 418-E-E-419 and 427-E-E-428. 1 Publication | 1 | |
Mutagenesisi | 418 – 419 | KK → EE: Decreased actin-binding. Loss of actin binding; when associated with D-28 and 427-E-E-428. 1 Publication | 2 | |
Mutagenesisi | 427 – 428 | KK → EE: Decreased actin-binding. Loss of actin binding; when associated with D-28 and 418-E-E-419. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050927 | 1 – 474 | Coronin-1CAdd BLAST | 474 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 446 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
CPTACi | non-CPTAC-3699 non-CPTAC-3780 |
EPDi | Q9WUM4 |
jPOSTi | Q9WUM4 |
MaxQBi | Q9WUM4 |
PaxDbi | Q9WUM4 |
PRIDEi | Q9WUM4 |
PTM databases
iPTMneti | Q9WUM4 |
PhosphoSitePlusi | Q9WUM4 |
SwissPalmi | Q9WUM4 |
Expressioni
Tissue specificityi
Detected in skeletal muscle (at protein level) (PubMed:19651142). Detected in fibroblasts (at protein level) (PubMed:27178841). Ubiquitous (PubMed:9778037).3 Publications
Gene expression databases
Bgeei | ENSMUSG00000004530, Expressed in placenta and 300 other tissues |
Genevisiblei | Q9WUM4, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- actin filament binding Source: MGI
- Rac GTPase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 204714, 162 interactors |
IntActi | Q9WUM4, 152 interactors |
MINTi | Q9WUM4 |
STRINGi | 10090.ENSMUSP00000004646 |
Miscellaneous databases
RNActi | Q9WUM4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 78 – 118 | WD 1Add BLAST | 41 | |
Repeati | 128 – 168 | WD 2Add BLAST | 41 | |
Repeati | 172 – 202 | WD 3Add BLAST | 31 | |
Repeati | 215 – 249 | WD 4Add BLAST | 35 | |
Repeati | 263 – 303 | WD 5Add BLAST | 41 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 435 – 474 | By similarityAdd BLAST | 40 |
Domaini
The C-terminal coiled-coil domain is essential for cortical membrane localization and oligomerization.By similarity
Sequence similaritiesi
Belongs to the WD repeat coronin family.Curated
Keywords - Domaini
Coiled coil, Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0303, Eukaryota |
GeneTreei | ENSGT00940000156488 |
HOGENOMi | CLU_026859_0_1_1 |
InParanoidi | Q9WUM4 |
OMAi | KVFIWEV |
OrthoDBi | 552726at2759 |
PhylomeDBi | Q9WUM4 |
TreeFami | TF314280 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR027333, CORO1C IPR015505, Coronin IPR015048, DUF1899 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR10856, PTHR10856, 1 hit PTHR10856:SF10, PTHR10856:SF10, 1 hit |
Pfami | View protein in Pfam PF08953, DUF1899, 1 hit PF00400, WD40, 3 hits |
SMARTi | View protein in SMART SM01166, DUF1899, 1 hit SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9WUM4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRVVRQSKF RHVFGQAVKN DQCYDDIRVS RVTWDSSFCA VNPRFVAIII
60 70 80 90 100
EASGGGAFLV LPLHKTGRID KSYPTVCGHT GPVLDIDWCP HNDQVIASGS
110 120 130 140 150
EDCTVMVWQI PENGLTLSLT EPVVILEGHS KRVGIVAWHP TARNVLLSAG
160 170 180 190 200
CDNAIIIWNV GTGEALINLD DMHSDMIYNV SWSRNGSLIC TASKDKKVRV
210 220 230 240 250
IDPRKQEIVA EKEKAHEGAR PMRAIFLADG NVFTTGFSRM SERQLALWNP
260 270 280 290 300
KNMQEPIALH EMDTSNGVLL PFYDPDTSII YLCGKGDSSI RYFEITDESP
310 320 330 340 350
YVHYLNTFSS KEPQRGMGYM PKRGLDVNKC EIARFFKLHE RKCEPIIMTV
360 370 380 390 400
PRKSDLFQDD LYPDTAGPEA ALEAEEWFEG KNADPILISL KHGYIPGKNR
410 420 430 440 450
DLKVVKKNIL DSKPAANKKS ELSCAPKKPT DTASVQNEAK LDEILKEIKS
460 470
IKETICSQDE RISKLEQQLA KMAA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PX03 | E9PX03_MOUSE | Coronin | Coro1c | 159 | Annotation score: | ||
E9PVJ1 | E9PVJ1_MOUSE | Coronin-1C | Coro1c | 85 | Annotation score: | ||
E9PZJ0 | E9PZJ0_MOUSE | Coronin-1C | Coro1c | 66 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 66 | T → S in AAD32705 (PubMed:9778037).Curated | 1 | |
Sequence conflicti | 422 | L → F in AAD32705 (PubMed:9778037).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF143957 mRNA Translation: AAD32705.1 AK079327 mRNA Translation: BAC37609.1 AK082986 mRNA Translation: BAC38722.1 AK088101 mRNA Translation: BAC40144.1 AK151017 mRNA Translation: BAE30036.1 AK151749 mRNA Translation: BAE30659.1 AK167089 mRNA Translation: BAE39246.1 AK159506 mRNA Translation: BAE35139.1 CH466529 Genomic DNA Translation: EDL19932.1 BC019444 mRNA Translation: AAH19444.1 BC085498 mRNA Translation: AAH85498.1 BC099671 mRNA Translation: AAH99671.1 |
CCDSi | CCDS19555.1 |
RefSeqi | NP_035909.2, NM_011779.3 |
Genome annotation databases
Ensembli | ENSMUST00000004646; ENSMUSP00000004646; ENSMUSG00000004530 |
GeneIDi | 23790 |
KEGGi | mmu:23790 |
UCSCi | uc008yyu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF143957 mRNA Translation: AAD32705.1 AK079327 mRNA Translation: BAC37609.1 AK082986 mRNA Translation: BAC38722.1 AK088101 mRNA Translation: BAC40144.1 AK151017 mRNA Translation: BAE30036.1 AK151749 mRNA Translation: BAE30659.1 AK167089 mRNA Translation: BAE39246.1 AK159506 mRNA Translation: BAE35139.1 CH466529 Genomic DNA Translation: EDL19932.1 BC019444 mRNA Translation: AAH19444.1 BC085498 mRNA Translation: AAH85498.1 BC099671 mRNA Translation: AAH99671.1 |
CCDSi | CCDS19555.1 |
RefSeqi | NP_035909.2, NM_011779.3 |
3D structure databases
SMRi | Q9WUM4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204714, 162 interactors |
IntActi | Q9WUM4, 152 interactors |
MINTi | Q9WUM4 |
STRINGi | 10090.ENSMUSP00000004646 |
PTM databases
iPTMneti | Q9WUM4 |
PhosphoSitePlusi | Q9WUM4 |
SwissPalmi | Q9WUM4 |
Proteomic databases
CPTACi | non-CPTAC-3699 non-CPTAC-3780 |
EPDi | Q9WUM4 |
jPOSTi | Q9WUM4 |
MaxQBi | Q9WUM4 |
PaxDbi | Q9WUM4 |
PRIDEi | Q9WUM4 |
Protocols and materials databases
Antibodypediai | 30774, 271 antibodies |
DNASUi | 23790 |
Genome annotation databases
Ensembli | ENSMUST00000004646; ENSMUSP00000004646; ENSMUSG00000004530 |
GeneIDi | 23790 |
KEGGi | mmu:23790 |
UCSCi | uc008yyu.1, mouse |
Organism-specific databases
CTDi | 23603 |
MGIi | MGI:1345964, Coro1c |
Phylogenomic databases
eggNOGi | KOG0303, Eukaryota |
GeneTreei | ENSGT00940000156488 |
HOGENOMi | CLU_026859_0_1_1 |
InParanoidi | Q9WUM4 |
OMAi | KVFIWEV |
OrthoDBi | 552726at2759 |
PhylomeDBi | Q9WUM4 |
TreeFami | TF314280 |
Miscellaneous databases
BioGRID-ORCSi | 23790, 1 hit in 19 CRISPR screens |
ChiTaRSi | Coro1c, mouse |
PROi | PR:Q9WUM4 |
RNActi | Q9WUM4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004530, Expressed in placenta and 300 other tissues |
Genevisiblei | Q9WUM4, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR027333, CORO1C IPR015505, Coronin IPR015048, DUF1899 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR10856, PTHR10856, 1 hit PTHR10856:SF10, PTHR10856:SF10, 1 hit |
Pfami | View protein in Pfam PF08953, DUF1899, 1 hit PF00400, WD40, 3 hits |
SMARTi | View protein in SMART SM01166, DUF1899, 1 hit SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COR1C_MOUSE | |
Accessioni | Q9WUM4Primary (citable) accession number: Q9WUM4 Secondary accession number(s): Q499X7, Q8VCQ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | October 11, 2004 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families