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Protein

ADP-ribosylation factor-like protein 3

Gene

Arl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (By similarity). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31Magnesium1
Metal bindingi48Magnesium1
Binding sitei48GTP1 Publication1
Binding sitei70GTP; via amide nitrogen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTP1 Publication8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTP1 Publication4
Nucleotide bindingi159 – 161GTP1 Publication3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Protein transport, Transport
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5624138 Trafficking of myristoylated proteins to the cilium

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 3
Gene namesi
Name:Arl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1929699 Arl3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Lethality by 3 weeks of age. Mice exhibit abnormal development of renal, hepatic, and pancreatic epithelial tubule structures. Mice show abnormal epithelial cell proliferation and cyst formation. Moreover, they exhibit photoreceptor degeneration as early as postnatal day 14.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31T → N: Inhibits interaction with PDE6D. 1 Publication1
Mutagenesisi49Q → L: Does not reduce the interaction with RP2. 1 Publication1
Mutagenesisi71Q → L: Does not inhibit interaction with PDE6D. 2 Publications1
Mutagenesisi71Q → L: Inhibits RP2-dependent GTP-hydrolysis rate. 2 Publications1
Mutagenesisi98K → Q: Does not reduce the interaction with RP2. 1 Publication1
Mutagenesisi164E → A: Reduces the interaction with RP2; when associated with A-168. 1 Publication1
Mutagenesisi168D → A: Reduces the interaction with RP2; when associated with A-164. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074572 – 182ADP-ribosylation factor-like protein 3Add BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei5PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ9WUL7
MaxQBiQ9WUL7
PaxDbiQ9WUL7
PRIDEiQ9WUL7

2D gel databases

REPRODUCTION-2DPAGEiIPI00124787
Q9WUL7

PTM databases

iPTMnetiQ9WUL7
PhosphoSitePlusiQ9WUL7
SwissPalmiQ9WUL7

Expressioni

Gene expression databases

BgeeiENSMUSG00000025035 Expressed in 283 organ(s), highest expression level in striatum
CleanExiMM_ARL3
ExpressionAtlasiQ9WUL7 baseline and differential
GenevisibleiQ9WUL7 MM

Interactioni

Subunit structurei

Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B). Interacts with SYS1. Interacts with ARL2BP; the GTP-bound form interacts with ARL2BP. Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3. Microtubule-associated protein. Does not interact with TBCC (By similarity). Interacts with RP2 and PDE6D. Interacts with PDE6D; the interaction occurs specifically with the GTP-bound form of ARL3.By similarity4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207916, 2 interactors
CORUMiQ9WUL7
DIPiDIP-29025N
IntActiQ9WUL7, 9 interactors
MINTiQ9WUL7
STRINGi10090.ENSMUSP00000026009

Structurei

Secondary structure

1182
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9WUL7
SMRiQ9WUL7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WUL7

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0074 Eukaryota
ENOG410Y825 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiQ9WUL7
KOiK07944
OMAiSEDITQV
OrthoDBiEOG091G0O6V
PhylomeDBiQ9WUL7
TreeFamiTF105463

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUL7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG
60 70 80 90 100
FNIKSVQSQG FKLNVWDIGG QRKIRPYWRS YFENTDILIY VIDSADRKRF
110 120 130 140 150
EETGQELTEL LEEEKLSCVP VLIFANKQDL LTAAPASEIA EGLNLHTIRD
160 170 180
RVWQIQSCSA LTGEGVQDGM NWVCKNVNAK KK
Length:182
Mass (Da):20,487
Last modified:November 1, 1999 - v1
Checksum:i16B85B2268ADB9DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143241 mRNA Translation: AAD33067.1
BC042941 mRNA Translation: AAH42941.1
CCDSiCCDS38010.1
RefSeqiNP_062692.1, NM_019718.2
UniGeneiMm.22085

Genome annotation databases

EnsembliENSMUST00000026009; ENSMUSP00000026009; ENSMUSG00000025035
GeneIDi56350
KEGGimmu:56350
UCSCiuc008htt.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143241 mRNA Translation: AAD33067.1
BC042941 mRNA Translation: AAH42941.1
CCDSiCCDS38010.1
RefSeqiNP_062692.1, NM_019718.2
UniGeneiMm.22085

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FZQX-ray1.70A2-182[»]
3BH6X-ray2.60A17-177[»]
3BH7X-ray1.90A17-177[»]
4GOJX-ray2.10A/B1-182[»]
4ZI2X-ray2.20A/B1-182[»]
4ZI3X-ray2.00A/B1-182[»]
ProteinModelPortaliQ9WUL7
SMRiQ9WUL7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207916, 2 interactors
CORUMiQ9WUL7
DIPiDIP-29025N
IntActiQ9WUL7, 9 interactors
MINTiQ9WUL7
STRINGi10090.ENSMUSP00000026009

PTM databases

iPTMnetiQ9WUL7
PhosphoSitePlusiQ9WUL7
SwissPalmiQ9WUL7

2D gel databases

REPRODUCTION-2DPAGEiIPI00124787
Q9WUL7

Proteomic databases

EPDiQ9WUL7
MaxQBiQ9WUL7
PaxDbiQ9WUL7
PRIDEiQ9WUL7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026009; ENSMUSP00000026009; ENSMUSG00000025035
GeneIDi56350
KEGGimmu:56350
UCSCiuc008htt.1 mouse

Organism-specific databases

CTDi403
MGIiMGI:1929699 Arl3

Phylogenomic databases

eggNOGiKOG0074 Eukaryota
ENOG410Y825 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiQ9WUL7
KOiK07944
OMAiSEDITQV
OrthoDBiEOG091G0O6V
PhylomeDBiQ9WUL7
TreeFamiTF105463

Enzyme and pathway databases

ReactomeiR-MMU-5624138 Trafficking of myristoylated proteins to the cilium

Miscellaneous databases

EvolutionaryTraceiQ9WUL7
PROiPR:Q9WUL7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025035 Expressed in 283 organ(s), highest expression level in striatum
CleanExiMM_ARL3
ExpressionAtlasiQ9WUL7 baseline and differential
GenevisibleiQ9WUL7 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARL3_MOUSE
AccessioniPrimary (citable) accession number: Q9WUL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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