UniProtKB - Q9WUL6 (M3K14_MOUSE)
Mitogen-activated protein kinase kinase kinase 14
Map3k14
Functioni
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
1 PublicationCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 431 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 517 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 408 – 416 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase kinase kinase activity Source: UniProtKB-EC
- NF-kappaB-inducing kinase activity Source: UniProtKB
- protein kinase activity Source: MGI
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: MGI
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cellular response to mechanical stimulus Source: Ensembl
- defense response to virus Source: MGI
- I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- immune response Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
- protein phosphorylation Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-389357, CD28 dependent PI3K/Akt signaling R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5676594, TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase kinase kinase 14 (EC:2.7.11.25)Alternative name(s): NF-kappa-beta-inducing kinase Serine/threonine-protein kinase NIK |
Gene namesi | Name:Map3k14 Synonyms:Nik |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1858204, Map3k14 |
VEuPathDBi | HostDB:ENSMUSG00000020941 |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: MGI
Nucleus
- fibrillar center Source: MGI
- nucleoplasm Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 855 | G → R in ALY; no binding to IKKA. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL4523500 |
GuidetoPHARMACOLOGYi | 2074 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086267 | 1 – 942 | Mitogen-activated protein kinase kinase kinase 14Add BLAST | 942 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 561 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q9WUL6 |
PRIDEi | Q9WUL6 |
PTM databases
iPTMneti | Q9WUL6 |
PhosphoSitePlusi | Q9WUL6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020941, Expressed in granulocyte and 176 other tissues |
ExpressionAtlasi | Q9WUL6, baseline and differential |
Genevisiblei | Q9WUL6, MM |
Interactioni
Subunit structurei
Interacts with TRAF2, TRAF3, TRAF5, TRAF6, IKKA and NF-kappa-B2/P100.
Interacts with PELI3.
Interacts with NIBP; the interaction is direct.
Interacts with ARRB1 and ARRB2.
Interacts with GRB10.
Interacts with ZFP91 (By similarity).
Interacts with NLRP12; this interaction promotes proteasomal degradation of MAP3K14. Directly interacts with DDX3X (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 207493, 3 interactors |
IntActi | Q9WUL6, 2 interactors |
STRINGi | 10090.ENSMUSP00000021324 |
Miscellaneous databases
RNActi | Q9WUL6, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9WUL6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 402 – 657 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 136 – 156 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 291 – 326 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 403 – 655 | Interaction with ZFP91By similarityAdd BLAST | 253 | |
Regioni | 660 – 756 | DisorderedSequence analysisAdd BLAST | 97 | |
Regioni | 801 – 823 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 136 – 153 | Basic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 703 – 721 | Pro residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0198, Eukaryota |
GeneTreei | ENSGT00940000156497 |
HOGENOMi | CLU_010641_1_0_1 |
InParanoidi | Q9WUL6 |
OMAi | LNWGKEE |
OrthoDBi | 162528at2759 |
PhylomeDBi | Q9WUL6 |
TreeFami | TF105120 |
Family and domain databases
CDDi | cd13991, STKc_NIK, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR042787, M3K14_STKc IPR017425, MAPKKK14 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR48016:SF9, PTHR48016:SF9, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF038175, MAPKKK14, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAVMEVACPG TPGSAVGQQK ELAKAKEKTQ SLGKKQSCIF KLEAVEKSPV
60 70 80 90 100
FCGKWEILND VITKGTAKDG SEGGPPAISI IAQAECENSQ EFSPTFSERI
110 120 130 140 150
FIAGSQQYSQ SESLDQIPNN VAHATEGKMA RVCRRGKRHG KARKKRRKKR
160 170 180 190 200
SKSLAQAGVA LAKPLPRTPE QESCTIPVQE DESPLGNLYA RNVSQFTKPL
210 220 230 240 250
GGPGLGHLCF KKQDEGLRPV LPRPELHKLI SPLQCLNHVW KLHHPQATGP
260 270 280 290 300
RPHPTHPFPY SGMPHPFPFY PLEPWKPYML DSAVLDKLAG VSGQRPLPGP
310 320 330 340 350
PHLSQLAHGD SQKPLPGPHL ESSCPSRGAL EKVPVEEYLV HALQGSVSSG
360 370 380 390 400
QAHSLASLAK TWSSGSAKLQ RLGPETEDNE GVLLTEKLKP VDYEYREEVH
410 420 430 440 450
WMTHQPRVGR GSFGEVHRMK DKQTGFQCAV KKVRLEVFRV EELVACAGLS
460 470 480 490 500
SPRIVPLYGA VREGPWVNIF MELLEGGSLG QLIKQMGCLP EDRALYYLGQ
510 520 530 540 550
ALEGLEYLHT RRILHGDVKA DNVLLSSDGS RAALCDFGHA LCLQPDGLGK
560 570 580 590 600
SLLTGDYIPG TETHMAPEVV MGKPCDAKVD IWSSCCMMLH MLNGCHPWTQ
610 620 630 640 650
YFRGPLCLKI ASEPPPIREI PPSCAPLTAQ AIQEGLRKEP VHRASAMELR
660 670 680 690 700
RKVGKALQEV GGLKSPWKGE YKEPRPPPQD QATCHQTLPT PPRENPPAKA
710 720 730 740 750
NTDGAPEPQP PLPPEPPEPS KAPALNLSKE ESGTWEPLPL SSLDPATAKG
760 770 780 790 800
PSFPDRRATL PELELQQLEI ELFLNSLSQP FSLEEQEQIL SCLSIDSLSL
810 820 830 840 850
SDDSEKNPSK ASQSSRDTLS SGVHSWNSQA EARTCSCSTA LARGRPTDIP
860 870 880 890 900
SYFNGVKVQI QSLNGEHLHI REFHRVKVGD IATGISSQIP ATAFSLVTKD
910 920 930 940
GQPVCYDMEV PDSGIDLQCT LAPDGSFAWT WRVKHGQLEN RP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF143094 mRNA Translation: AAD31512.1 |
CCDSi | CCDS25516.1 |
RefSeqi | NP_058592.1, NM_016896.3 |
Genome annotation databases
Ensembli | ENSMUST00000021324; ENSMUSP00000021324; ENSMUSG00000020941 |
GeneIDi | 53859 |
KEGGi | mmu:53859 |
UCSCi | uc007lua.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF143094 mRNA Translation: AAD31512.1 |
CCDSi | CCDS25516.1 |
RefSeqi | NP_058592.1, NM_016896.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4G3C | X-ray | 2.15 | A/B | 329-675 | [»] | |
4G3E | X-ray | 2.50 | A/B | 329-675 | [»] | |
4G3F | X-ray | 1.64 | A | 345-675 | [»] | |
4G3G | X-ray | 2.50 | A | 345-675 | [»] | |
5T8O | X-ray | 2.41 | A/B | 329-675 | [»] | |
5T8P | X-ray | 2.32 | A/B | 329-675 | [»] | |
5T8Q | X-ray | 2.63 | A/B | 329-675 | [»] | |
6G4Y | X-ray | 2.65 | A/B | 329-675 | [»] | |
6G4Z | X-ray | 2.84 | A/B | 329-675 | [»] | |
6MYN | X-ray | 2.74 | A/B | 329-675 | [»] | |
SMRi | Q9WUL6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 207493, 3 interactors |
IntActi | Q9WUL6, 2 interactors |
STRINGi | 10090.ENSMUSP00000021324 |
Chemistry databases
ChEMBLi | CHEMBL4523500 |
GuidetoPHARMACOLOGYi | 2074 |
PTM databases
iPTMneti | Q9WUL6 |
PhosphoSitePlusi | Q9WUL6 |
Proteomic databases
PaxDbi | Q9WUL6 |
PRIDEi | Q9WUL6 |
Protocols and materials databases
Antibodypediai | 3862, 350 antibodies from 39 providers |
DNASUi | 53859 |
Genome annotation databases
Ensembli | ENSMUST00000021324; ENSMUSP00000021324; ENSMUSG00000020941 |
GeneIDi | 53859 |
KEGGi | mmu:53859 |
UCSCi | uc007lua.1, mouse |
Organism-specific databases
CTDi | 9020 |
MGIi | MGI:1858204, Map3k14 |
VEuPathDBi | HostDB:ENSMUSG00000020941 |
Phylogenomic databases
eggNOGi | KOG0198, Eukaryota |
GeneTreei | ENSGT00940000156497 |
HOGENOMi | CLU_010641_1_0_1 |
InParanoidi | Q9WUL6 |
OMAi | LNWGKEE |
OrthoDBi | 162528at2759 |
PhylomeDBi | Q9WUL6 |
TreeFami | TF105120 |
Enzyme and pathway databases
Reactomei | R-MMU-389357, CD28 dependent PI3K/Akt signaling R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5676594, TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway |
Miscellaneous databases
BioGRID-ORCSi | 53859, 2 hits in 63 CRISPR screens |
PROi | PR:Q9WUL6 |
RNActi | Q9WUL6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020941, Expressed in granulocyte and 176 other tissues |
ExpressionAtlasi | Q9WUL6, baseline and differential |
Genevisiblei | Q9WUL6, MM |
Family and domain databases
CDDi | cd13991, STKc_NIK, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR042787, M3K14_STKc IPR017425, MAPKKK14 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR48016:SF9, PTHR48016:SF9, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF038175, MAPKKK14, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | M3K14_MOUSE | |
Accessioni | Q9WUL6Primary (citable) accession number: Q9WUL6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families