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UniProtKB - Q9WUL6 (M3K14_MOUSE)

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Protein

Mitogen-activated protein kinase kinase kinase 14

Gene

Map3k14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei431ATPPROSITE-ProRule annotation1
Active sitei517Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi408 – 416ATPPROSITE-ProRule annotation9

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-389357 CD28 dependent PI3K/Akt signaling
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 14 (EC:2.7.11.25)
Alternative name(s):
NF-kappa-beta-inducing kinase
Serine/threonine-protein kinase NIK
Gene namesi
Name:Map3k14
Synonyms:Nik
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1858204 Map3k14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice display the alymphoplasia phenotype (aly), which is characterized by systemic absence of lymph nodes and Peyer patches and disorganized splenic and thymic structures with immunodeficiency.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi855G → R in ALY; no binding to IKKA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862671 – 942Mitogen-activated protein kinase kinase kinase 14Add BLAST942

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei561PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation at Thr-561 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization (By similarity). Autophosphorylated.By similarity
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9WUL6
PRIDEiQ9WUL6

PTM databases

iPTMnetiQ9WUL6
PhosphoSitePlusiQ9WUL6

Expressioni

Gene expression databases

BgeeiENSMUSG00000020941
CleanExiMM_MAP3K14
ExpressionAtlasiQ9WUL6 baseline and differential
GenevisibleiQ9WUL6 MM

Interactioni

Subunit structurei

Interacts with TRAF2, TRAF3, TRAF5, TRAF6, IKKA and NF-kappa-B2/P100. Interacts with PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91 (By similarity).By similarity

Protein-protein interaction databases

BioGridi207493, 3 interactors
IntActiQ9WUL6, 2 interactors
STRINGi10090.ENSMUSP00000021324

Structurei

Secondary structure

1942
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi335 – 343Combined sources9
Beta strandi346 – 349Combined sources4
Helixi352 – 359Combined sources8
Turni360 – 362Combined sources3
Beta strandi379 – 383Combined sources5
Turni397 – 399Combined sources3
Beta strandi400 – 411Combined sources12
Beta strandi414 – 421Combined sources8
Turni422 – 424Combined sources3
Beta strandi427 – 434Combined sources8
Turni435 – 437Combined sources3
Helixi441 – 444Combined sources4
Turni445 – 448Combined sources4
Beta strandi457 – 463Combined sources7
Beta strandi466 – 471Combined sources6
Helixi479 – 486Combined sources8
Helixi491 – 509Combined sources19
Turni510 – 512Combined sources3
Helixi520 – 522Combined sources3
Beta strandi523 – 525Combined sources3
Beta strandi532 – 534Combined sources3
Turni545 – 547Combined sources3
Beta strandi550 – 552Combined sources3
Beta strandi553 – 556Combined sources4
Helixi562 – 564Combined sources3
Helixi567 – 570Combined sources4
Helixi578 – 593Combined sources16
Turni599 – 601Combined sources3
Helixi607 – 612Combined sources6
Helixi616 – 619Combined sources4
Helixi626 – 635Combined sources10
Helixi640 – 642Combined sources3
Helixi646 – 659Combined sources14
Beta strandi667 – 670Combined sources4

3D structure databases

ProteinModelPortaliQ9WUL6
SMRiQ9WUL6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini402 – 657Protein kinasePROSITE-ProRule annotationAdd BLAST256

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni403 – 655Interaction with ZFP91By similarityAdd BLAST253

Sequence similaritiesi

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Curated

Phylogenomic databases

eggNOGiKOG0198 Eukaryota
ENOG410XQGS LUCA
GeneTreeiENSGT00800000124036
HOGENOMiHOG000113436
HOVERGENiHBG052384
InParanoidiQ9WUL6
KOiK04466
OMAiYDMEVPD
OrthoDBiEOG091G018K
PhylomeDBiQ9WUL6
TreeFamiTF105120

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR017425 MAPKKK14
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24361:SF226 PTHR24361:SF226, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF038175 MAPKKK14, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WUL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVMEVACPG TPGSAVGQQK ELAKAKEKTQ SLGKKQSCIF KLEAVEKSPV 
        60         70         80         90        100
FCGKWEILND VITKGTAKDG SEGGPPAISI IAQAECENSQ EFSPTFSERI 
       110        120        130        140        150
FIAGSQQYSQ SESLDQIPNN VAHATEGKMA RVCRRGKRHG KARKKRRKKR 
       160        170        180        190        200
SKSLAQAGVA LAKPLPRTPE QESCTIPVQE DESPLGNLYA RNVSQFTKPL 
       210        220        230        240        250
GGPGLGHLCF KKQDEGLRPV LPRPELHKLI SPLQCLNHVW KLHHPQATGP 
       260        270        280        290        300
RPHPTHPFPY SGMPHPFPFY PLEPWKPYML DSAVLDKLAG VSGQRPLPGP 
       310        320        330        340        350
PHLSQLAHGD SQKPLPGPHL ESSCPSRGAL EKVPVEEYLV HALQGSVSSG 
       360        370        380        390        400
QAHSLASLAK TWSSGSAKLQ RLGPETEDNE GVLLTEKLKP VDYEYREEVH 
       410        420        430        440        450
WMTHQPRVGR GSFGEVHRMK DKQTGFQCAV KKVRLEVFRV EELVACAGLS 
       460        470        480        490        500
SPRIVPLYGA VREGPWVNIF MELLEGGSLG QLIKQMGCLP EDRALYYLGQ 
       510        520        530        540        550
ALEGLEYLHT RRILHGDVKA DNVLLSSDGS RAALCDFGHA LCLQPDGLGK 
       560        570        580        590        600
SLLTGDYIPG TETHMAPEVV MGKPCDAKVD IWSSCCMMLH MLNGCHPWTQ 
       610        620        630        640        650
YFRGPLCLKI ASEPPPIREI PPSCAPLTAQ AIQEGLRKEP VHRASAMELR 
       660        670        680        690        700
RKVGKALQEV GGLKSPWKGE YKEPRPPPQD QATCHQTLPT PPRENPPAKA 
       710        720        730        740        750
NTDGAPEPQP PLPPEPPEPS KAPALNLSKE ESGTWEPLPL SSLDPATAKG 
       760        770        780        790        800
PSFPDRRATL PELELQQLEI ELFLNSLSQP FSLEEQEQIL SCLSIDSLSL 
       810        820        830        840        850
SDDSEKNPSK ASQSSRDTLS SGVHSWNSQA EARTCSCSTA LARGRPTDIP 
       860        870        880        890        900
SYFNGVKVQI QSLNGEHLHI REFHRVKVGD IATGISSQIP ATAFSLVTKD 
       910        920        930        940 
GQPVCYDMEV PDSGIDLQCT LAPDGSFAWT WRVKHGQLEN RP
Length:942
Mass (Da):103,080
Last modified:November 1, 1999 - v1
Checksum:i3BE4E4BA2D25C200
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143094 mRNA Translation: AAD31512.1
CCDSiCCDS25516.1
RefSeqiNP_058592.1, NM_016896.3
UniGeneiMm.158981

Genome annotation databases

EnsembliENSMUST00000021324; ENSMUSP00000021324; ENSMUSG00000020941
GeneIDi53859
KEGGimmu:53859
UCSCiuc007lua.1 mouse

Similar proteinsi

Entry informationi

Entry nameiM3K14_MOUSE
AccessioniPrimary (citable) accession number: Q9WUL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1999
Last modified: June 20, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

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