UniProtKB - Q9WUK7 (OPN3_MOUSE)
Protein
Opsin-3
Gene
Opn3
Organism
Mus musculus (Mouse)
Status
Functioni
G-protein coupled receptor which selectively activates G proteins via ultraviolet A (UVA) light-mediated activation in the skin (PubMed:30284927). Binds both 11-cis retinal and all-trans retinal (By similarity). Regulates melanogenesis in melanocytes via inhibition of alpha-MSH-induced MC1R-mediated cAMP signaling, modulation of calcium flux, regulation of CAMK2 phosphorylation, and subsequently phosphorylation of CREB, p38, ERK and MITF in response to blue light (By similarity). Plays a role in melanocyte survival through regulation of intracellular calcium levels and subsequent BCL2/RAF1 signaling (By similarity). Additionally regulates apoptosis via cytochrome c release and subsequent activation of the caspase cascade (By similarity). Required for TYR and DCT blue light-induced complex formation in melanocytes (By similarity). Involved in keratinocyte differentiation in response to blue-light (By similarity). Required for the UVA-mediated induction of calcium and mitogen-activated protein kinase signaling resulting in the expression of MMP1, MMP2, MMP3, MMP9 and TIMP1 in dermal fibroblasts (By similarity). Plays a role in light-mediated glucose uptake, mitochondrial respiration and fatty acid metabolism in brown adipocyte tissues (PubMed:32040503). May be involved in photorelaxation of airway smooth muscle cells, via blue-light dependent GPCR signaling pathways (PubMed:30284927).By similarity2 Publications
GO - Molecular functioni
- 11-cis retinal binding Source: UniProtKB
- all-trans retinal binding Source: UniProtKB
- G protein-coupled photoreceptor activity Source: GO_Central
GO - Biological processi
- cellular response to light stimulus Source: GO_Central
- cellular response to UV-A Source: MGI
- G protein-coupled receptor signaling pathway Source: GO_Central
- keratinocyte differentiation Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of melanin biosynthetic process Source: UniProtKB
- phototransduction Source: GO_Central
- positive regulation of cellular respiration Source: UniProtKB
- positive regulation of glucose import Source: UniProtKB
- positive regulation of melanin biosynthetic process Source: UniProtKB
- protein-chromophore linkage Source: UniProtKB-KW
- response to blue light Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer |
Biological process | |
Ligand | Chromophore |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events R-MMU-419771, Opsins |
Names & Taxonomyi
Protein namesi | Recommended name: Opsin-3Alternative name(s): Encephalopsin Panopsin |
Gene namesi | Name:Opn3 Synonyms:Ecpn |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1338022, Opn3 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Other locations
- Cytoplasm By similarity
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- photoreceptor outer segment Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 38 | ExtracellularSequence analysisAdd BLAST | 38 | |
Transmembranei | 39 – 63 | Helical; Name=1Sequence analysisAdd BLAST | 25 | |
Topological domaini | 64 – 75 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 76 – 100 | Helical; Name=2Sequence analysisAdd BLAST | 25 | |
Topological domaini | 101 – 115 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 116 – 135 | Helical; Name=3Sequence analysisAdd BLAST | 20 | |
Topological domaini | 136 – 151 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 152 – 175 | Helical; Name=4Sequence analysisAdd BLAST | 24 | |
Topological domaini | 176 – 199 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 200 – 227 | Helical; Name=5Sequence analysisAdd BLAST | 28 | |
Topological domaini | 228 – 253 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 254 – 277 | Helical; Name=6Sequence analysisAdd BLAST | 24 | |
Topological domaini | 278 – 285 | ExtracellularSequence analysis | 8 | |
Transmembranei | 286 – 310 | Helical; Name=7Sequence analysisAdd BLAST | 25 | |
Topological domaini | 311 – 400 | CytoplasmicSequence analysisAdd BLAST | 90 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype (PubMed:32040503). Increased weight gain on a high fat diet, as a result of increased fat mass and increased insulin resistance (PubMed:32040503). Increased basal glucose uptake in brown adipocytes, potentially as a result of decreased GLUT1 expression (PubMed:32040503). Significantly reduced lipocytic rate, mitochondrial DNA expression and cytochrome C oxidase activity in brown adipocyte tissues (PubMed:32040503). Loss of light-mediated increase in glucose uptake, mitochondrial respiration, thermogenic capacity and lipid metabolism-related gene expression in brown adipocyte tissues (PubMed:32040503). Impaired maximum thermogenic capacity with reduced heat production and reduced oxygen consumption in response to norepinephrine treatment in brown adipocytes (PubMed:32040503).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 136 – 138 | ERY → RDR: Reduced basal glucose uptake and glucose-dependent mitochondrial respiration. 1 Publication | 3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197814 | 1 – 400 | Opsin-3Add BLAST | 400 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 5 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 112 ↔ 186 | PROSITE-ProRule annotation | ||
Glycosylationi | 196 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 297 | N6-(retinylidene)lysine | 1 | |
Lipidationi | 323 | S-palmitoyl cysteineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, PalmitateProteomic databases
PaxDbi | Q9WUK7 |
PRIDEi | Q9WUK7 |
PTM databases
GlyGeni | Q9WUK7, 2 sites |
PhosphoSitePlusi | Q9WUK7 |
Expressioni
Tissue specificityi
Expressed in the eye (at protein level) (PubMed:30284927). Expressed in tracheal airway smooth muscle (PubMed:30284927). Expressed in brown adipocyte tissue; expression becomes more abundant during differentiation (PubMed:32040503). Strongly expressed in brain (PubMed:10234000). Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus (PubMed:10234000). Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord (PubMed:10234000).3 Publications
Developmental stagei
Expressed at substantial levels in the dorsal pons at 18.5 dpc (PubMed:10234000). Expressed in Purkinje cells at P4, with expression becoming striped at P20 (PubMed:10234000). Expressed in the cerebral cortex from 18.5 dpc with a rostrocaudal gradient of expression becoming evident at P20 (PubMed:10234000).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000026525, Expressed in brown adipose tissue and 224 other tissues |
Genevisiblei | Q9WUK7, MM |
Interactioni
Subunit structurei
Interacts with MC1R; the interaction results in a decrease in MC1R-mediated cAMP signaling and ultimately a decrease in melanin production in melanocytes.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000027809 |
Miscellaneous databases
RNActi | Q9WUK7, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00980000198536 |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | Q9WUK7 |
OMAi | VMFLLMI |
OrthoDBi | 940057at2759 |
PhylomeDBi | Q9WUK7 |
TreeFami | TF324998 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR027430, Retinal_BS |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9WUK7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYSGNRSGDQ GYWEDGAGAE GAAPAGTRSP APLFSPTAYE RLALLLGCLA
60 70 80 90 100
LLGVGGNLLV LLLYSKFPRL RTPTHLFLVN LSLGDLLVSL FGVTFTFASC
110 120 130 140 150
LRNGWVWDAV GCAWDGFSGS LFGFVSITTL TVLAYERYIR VVHARVINFS
160 170 180 190 200
WAWRAITYIW LYSLAWAGAP LLGWNRYILD IHGLGCTVDW RSKDANDSSF
210 220 230 240 250
VLFLFLGCLV VPVGIIAHCY GHILYSVRML RCVEDLQTIQ VIKMLRYEKK
260 270 280 290 300
VAKMCFLMAF VFLTCWMPYI VTRFLVVNGY GHLVTPTVSI VSYLFAKSST
310 320 330 340 350
VYNPVIYIFM NRKFRRSLLQ LLCFRLLRCQ RPAKNLPAAE SEMHIRPIVM
360 370 380 390 400
SQKDGDRPKK KVTFNSSSII FIITSDESLS VEDSDRSSAS KVDVIQVRPL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF140241 mRNA Translation: AAD32670.1 AF482427 Genomic DNA Translation: AAO15719.1 AK043135 mRNA Translation: BAC31471.1 AK132822 mRNA Translation: BAE21380.1 |
CCDSi | CCDS15549.1 |
RefSeqi | NP_034228.1, NM_010098.3 |
Genome annotation databases
Ensembli | ENSMUST00000027809; ENSMUSP00000027809; ENSMUSG00000026525 |
GeneIDi | 13603 |
KEGGi | mmu:13603 |
UCSCi | uc007dtq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF140241 mRNA Translation: AAD32670.1 AF482427 Genomic DNA Translation: AAO15719.1 AK043135 mRNA Translation: BAC31471.1 AK132822 mRNA Translation: BAE21380.1 |
CCDSi | CCDS15549.1 |
RefSeqi | NP_034228.1, NM_010098.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000027809 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q9WUK7, 2 sites |
PhosphoSitePlusi | Q9WUK7 |
Proteomic databases
PaxDbi | Q9WUK7 |
PRIDEi | Q9WUK7 |
Protocols and materials databases
Antibodypediai | 34703, 231 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027809; ENSMUSP00000027809; ENSMUSG00000026525 |
GeneIDi | 13603 |
KEGGi | mmu:13603 |
UCSCi | uc007dtq.2, mouse |
Organism-specific databases
CTDi | 23596 |
MGIi | MGI:1338022, Opn3 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00980000198536 |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | Q9WUK7 |
OMAi | VMFLLMI |
OrthoDBi | 940057at2759 |
PhylomeDBi | Q9WUK7 |
TreeFami | TF324998 |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events R-MMU-419771, Opsins |
Miscellaneous databases
BioGRID-ORCSi | 13603, 1 hit in 17 CRISPR screens |
ChiTaRSi | Opn3, mouse |
PROi | PR:Q9WUK7 |
RNActi | Q9WUK7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026525, Expressed in brown adipose tissue and 224 other tissues |
Genevisiblei | Q9WUK7, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR027430, Retinal_BS |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPN3_MOUSE | |
Accessioni | Q9WUK7Primary (citable) accession number: Q9WUK7 Secondary accession number(s): Q3V0X3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries