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Protein

Nuclear receptor coactivator 2

Gene

Ncoa2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processBiological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor coactivator 2
Short name:
NCoA-2
Alternative name(s):
Transcriptional intermediary factor 2
Gene namesi
Name:Ncoa2
Synonyms:Tif2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620108 Ncoa2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000944042 – 1465Nuclear receptor coactivator 2Add BLAST1464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei29PhosphoserineBy similarity1
Cross-linki239Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei338Asymmetric dimethylarginineBy similarity1
Modified residuei396PhosphoserineCombined sources1
Modified residuei487PhosphoserineBy similarity1
Modified residuei493PhosphoserineCombined sources1
Modified residuei499PhosphoserineCombined sources1
Modified residuei554PhosphoserineBy similarity1
Modified residuei565PhosphoserineBy similarity1
Modified residuei636N6-acetyllysineBy similarity1
Modified residuei640N6-acetyllysineBy similarity1
Cross-linki648Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei682PhosphoserineBy similarity1
Modified residuei699PhosphoserineCombined sources1
Cross-linki705Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki731Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei736PhosphoserineBy similarity1
Modified residuei771PhosphoserineBy similarity1
Modified residuei780N6-acetyllysineBy similarity1
Modified residuei785N6-acetyllysine; alternateBy similarity1
Cross-linki785Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei864Asymmetric dimethylarginineBy similarity1
Modified residuei874Asymmetric dimethylarginineBy similarity1
Modified residuei1173Asymmetric dimethylarginineBy similarity1
Modified residuei1177Asymmetric dimethylarginineBy similarity1
Modified residuei1190Asymmetric dimethylarginineBy similarity1
Modified residuei1196Asymmetric dimethylarginineBy similarity1
Modified residuei1203Asymmetric dimethylarginineBy similarity1
Modified residuei1221Asymmetric dimethylarginineBy similarity1
Modified residuei1240Asymmetric dimethylarginineBy similarity1
Modified residuei1261Omega-N-methylarginineBy similarity1
Modified residuei1266Asymmetric dimethylarginineBy similarity1
Cross-linki1455Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9WUI9
PRIDEiQ9WUI9

PTM databases

iPTMnetiQ9WUI9
PhosphoSitePlusiQ9WUI9

Interactioni

Subunit structurei

Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with ESR1, HIF1A, NCOA1, APEX, NR3C1, NR3C2, CARM1, RARA, and RXRA. Present in a complex containing CARM1 and EP300/P300. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with PSMB9 and DDX5. Interacts (via LXXLL 1, 2 and 3 motifs) with RORA and RORC (via AF-2 motif). Interacts with RWDD3. Interacts with CLOCK and ARNTL/BMAL1. Interacts with NR4A3; potentiates the activity of the NR4A3 (By similarity). Interacts with NR1H3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011026

Structurei

Secondary structure

11465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9WUI9
SMRiQ9WUI9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WUI9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 83bHLHPROSITE-ProRule annotationAdd BLAST58
Domaini119 – 183PASPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni691 – 743CASP8AP2-bindingAdd BLAST53
Regioni730 – 1121Interaction with ARNTLBy similarityAdd BLAST392

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi641 – 645LXXLL motif 15
Motifi690 – 694LXXLL motif 25
Motifi745 – 749LXXLL motif 35
Motifi878 – 882LXXLL motif 45
Motifi1079 – 1087LLXXLXXXL motif9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1254 – 1260Poly-Gln7

Domaini

Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant (By similarity).By similarity
The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQ5S Eukaryota
ENOG410XPF9 LUCA
HOGENOMiHOG000230947
HOVERGENiHBG052583
InParanoidiQ9WUI9
KOiK11255
PhylomeDBiQ9WUI9

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 1 hit
Gene3Di1.10.287.1070, 1 hit
4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR010011 DUF1518
IPR032565 DUF4927
IPR036638 HLH_DNA-bd_sf
IPR028822 NCOA2
IPR009110 Nuc_rcpt_coact
IPR014920 Nuc_rcpt_coact_Ncoa-typ
IPR037077 Nuc_rcpt_coact_Ncoa_int_sf
IPR017426 Nuclear_rcpt_coactivator
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR014935 SRC/p160_LXXLL
PANTHERiPTHR10684 PTHR10684, 1 hit
PTHR10684:SF2 PTHR10684:SF2, 1 hit
PfamiView protein in Pfam
PF07469 DUF1518, 1 hit
PF16279 DUF4927, 1 hit
PF08815 Nuc_rec_co-act, 1 hit
PF00989 PAS, 1 hit
PF08832 SRC-1, 1 hit
PIRSFiPIRSF038181 Nuclear_receptor_coactivator, 1 hit
SMARTiView protein in SMART
SM01151 DUF1518, 1 hit
SM00353 HLH, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
SSF69125 SSF69125, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9WUI9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGMGENTSD PSRAETRKRK ECPDQLGPSP KRSTEKRNRE QENKYIEELA
60 70 80 90 100
ELIFANFNDI DNFNFKPDKC AILKETVKQI RQIKEQEKAA AANIDEVQKS
110 120 130 140 150
DVSSTGQGVI DKDALGPMML EALDGFFFVV NLEGNVVFVS ENVTQYLRYN
160 170 180 190 200
QEELMNKSVY SILHVGDHTE FVKNLLPKSM VNGGSWTGEP PRRNSHTFNC
210 220 230 240 250
RMLVKPLPDS EEEGHDNQEA HQKYETMQCF AVSQPKSIKE EGEDKQSCLI
260 270 280 290 300
CVARRVPMKE RPALPSSESF TTRQDLQGKI TFLDTSTMRD AMKPGWEDLV
310 320 330 340 350
RRCIQKFHTQ HEGESLSYAK RHHHEVLRQG LAFSQIYRFS LSDGTLVAAQ
360 370 380 390 400
TKSKLIRSQT TNEPQLVISI HMLHREQNVC VMNPDLTGQA MGKPLSPMSS
410 420 430 440 450
SSPARQAMCS GNPGQDVALG SNMNFPMNGP REQMSMPMGR FGGSGGMNHV
460 470 480 490 500
SGMQATTPQG SNYALKMNSP SQSSPGLNPG QPSSVLSPRH RMSPGVAGSP
510 520 530 540 550
RVPPSQFSPA GSLHSPAGVC SSTGNSHSYT NSSLNALQAL SEGHGVSLGP
560 570 580 590 600
SLASPDLKMG NSQNSPVNMN PPPLSKMGSL DSKDCFGLYG EPSEGTTGQA
610 620 630 640 650
QASCHPEEQK RPNDSSMPQA ASEDRAEGHS RLHESKGQTK LLQLLTTKSD
660 670 680 690 700
QMEPSPLPSS LSDTNKDSTG SLPGPGSTHG TSLKEKHKIL HRLLQDSSSP
710 720 730 740 750
VDLAKLTAEA TGKELNQESS GTAPGSEVTV KQEPASPKKK ENALLRYLLD
760 770 780 790 800
KDDTKDIGLP EITPKLERLD SKTDPASNTK LIAMKTVKEE VSFEPSDQPG
810 820 830 840 850
SELDNLEEIL DDLQNSQLPQ LFPDTRPGAP TGSVDKQAII NDLMQLTADS
860 870 880 890 900
SPVTPVGAQK AALRMSQSTF NNPRPGQLGR LLPNQNLPLD ITLQSPTGAG
910 920 930 940 950
PFPPIRNSSP YSVIPQPGMM GNQGMLGSQG NLGNNSTGMI GSSTSRSSMP
960 970 980 990 1000
SGEWAPQSPA VRVTCAATTG AMNRPIQGGM IRNPTASIPM RANSQPGQRQ
1010 1020 1030 1040 1050
MLQPQVMNIG PSELEMNMGG PQYNQQQAPP NQTAPWPESI LPIDQASFGS
1060 1070 1080 1090 1100
QNRHPFGSSP DDLLCPHPAA ESPSDEGALL DQLYLALRNF DGLEEIDRAL
1110 1120 1130 1140 1150
GIPELVSQSQ AVDPEQFSSQ ESSMMLEQKP PVFPQQYASQ TQMAQGSYNP
1160 1170 1180 1190 1200
MQDPNFHTMG QRPNYTTLRM QPRPGLRPTG IVQNQPNQLR LQLQHRLQAQ
1210 1220 1230 1240 1250
QNRQPLMNQI SGVSNVNLTL RPGVPTQAPI NAQMLAQRQR EILNQHLRQR
1260 1270 1280 1290 1300
QMHQQQQVQQ RTLMMRGQGL NMTPSMVAPT GLPAAMSNPR IPQANAQQFP
1310 1320 1330 1340 1350
FPPNYGISQQ PDPGFTGATT PQSPLMSPRM AHTQSPMMQQ SQANPAYQPA
1360 1370 1380 1390 1400
SDINGWAQGS MGGNSMFSQQ SPPHFGQQAN TSMYNNNMNI NVSMATNTAG
1410 1420 1430 1440 1450
LSNMNQMTGQ MSMTSVTSVP TSGLSSMGPE QVNDPALRGS SLFTTNQLPG
1460
MDMIKQEGDG SRKYC
Length:1,465
Mass (Da):159,436
Last modified:November 1, 1999 - v1
Checksum:i36625B573EB0B39C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1MA61F1MA61_RAT
Nuclear receptor coactivator
Ncoa2
1,465Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136943 mRNA Translation: AAD24587.1
RefSeqiNP_114010.1, NM_031822.1
UniGeneiRn.49136

Genome annotation databases

GeneIDi83724
KEGGirno:83724
UCSCiRGD:620108 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136943 mRNA Translation: AAD24587.1
RefSeqiNP_114010.1, NM_031822.1
UniGeneiRn.49136

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XLSX-ray2.96I/J/K/L/M/N/O/P740-756[»]
3HLVX-ray3.00C/D686-698[»]
3HM1X-ray2.33C/D686-698[»]
3L03X-ray1.90C/D686-698[»]
ProteinModelPortaliQ9WUI9
SMRiQ9WUI9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011026

PTM databases

iPTMnetiQ9WUI9
PhosphoSitePlusiQ9WUI9

Proteomic databases

PaxDbiQ9WUI9
PRIDEiQ9WUI9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83724
KEGGirno:83724
UCSCiRGD:620108 rat

Organism-specific databases

CTDi10499
RGDi620108 Ncoa2

Phylogenomic databases

eggNOGiENOG410IQ5S Eukaryota
ENOG410XPF9 LUCA
HOGENOMiHOG000230947
HOVERGENiHBG052583
InParanoidiQ9WUI9
KOiK11255
PhylomeDBiQ9WUI9

Miscellaneous databases

EvolutionaryTraceiQ9WUI9
PROiPR:Q9WUI9

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 1 hit
Gene3Di1.10.287.1070, 1 hit
4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR010011 DUF1518
IPR032565 DUF4927
IPR036638 HLH_DNA-bd_sf
IPR028822 NCOA2
IPR009110 Nuc_rcpt_coact
IPR014920 Nuc_rcpt_coact_Ncoa-typ
IPR037077 Nuc_rcpt_coact_Ncoa_int_sf
IPR017426 Nuclear_rcpt_coactivator
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR014935 SRC/p160_LXXLL
PANTHERiPTHR10684 PTHR10684, 1 hit
PTHR10684:SF2 PTHR10684:SF2, 1 hit
PfamiView protein in Pfam
PF07469 DUF1518, 1 hit
PF16279 DUF4927, 1 hit
PF08815 Nuc_rec_co-act, 1 hit
PF00989 PAS, 1 hit
PF08832 SRC-1, 1 hit
PIRSFiPIRSF038181 Nuclear_receptor_coactivator, 1 hit
SMARTiView protein in SMART
SM01151 DUF1518, 1 hit
SM00353 HLH, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
SSF69125 SSF69125, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNCOA2_RAT
AccessioniPrimary (citable) accession number: Q9WUI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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