UniProtKB - Q9WUD1 (CHIP_MOUSE)
E3 ubiquitin-protein ligase CHIP
Stub1
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- chaperone binding Source: MGI
- enzyme binding Source: MGI
- G protein-coupled receptor binding Source: MGI
- heat shock protein binding Source: MGI
- Hsp70 protein binding Source: HGNC-UCL
- Hsp90 protein binding Source: BHF-UCL
- kinase binding Source: MGI
- misfolded protein binding Source: MGI
- protein homodimerization activity Source: HGNC-UCL
- protein-macromolecule adaptor activity Source: HGNC-UCL
- SMAD binding Source: HGNC-UCL
- TPR domain binding Source: HGNC-UCL
- ubiquitin protein ligase activity Source: MGI
- ubiquitin protein ligase binding Source: UniProtKB
- ubiquitin-protein transferase activity Source: UniProtKB
- ubiquitin-ubiquitin ligase activity Source: MGI
GO - Biological processi
- cellular response to heat Source: MGI
- cellular response to hypoxia Source: MGI
- cellular response to misfolded protein Source: UniProtKB
- chaperone-mediated autophagy Source: MGI
- DNA repair Source: UniProtKB-KW
- endoplasmic reticulum unfolded protein response Source: ParkinsonsUK-UCL
- negative regulation of protein binding Source: ParkinsonsUK-UCL
- negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
- positive regulation of chaperone-mediated protein complex assembly Source: MGI
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: HGNC-UCL
- positive regulation of protein ubiquitination Source: ParkinsonsUK-UCL
- positive regulation of proteolysis Source: GO_Central
- positive regulation of ubiquitin-protein transferase activity Source: ParkinsonsUK-UCL
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein autoubiquitination Source: UniProtKB
- protein folding Source: HGNC-UCL
- protein K63-linked ubiquitination Source: UniProtKB
- protein maturation Source: HGNC-UCL
- protein polyubiquitination Source: MGI
- protein quality control for misfolded or incompletely synthesized proteins Source: UniProtKB
- protein ubiquitination Source: MGI
- regulation of glucocorticoid metabolic process Source: HGNC-UCL
- regulation of protein stability Source: MGI
- response to ischemia Source: MGI
- ubiquitin-dependent ERAD pathway Source: MGI
- ubiquitin-dependent protein catabolic process Source: ARUK-UCL
- ubiquitin-dependent SMAD protein catabolic process Source: HGNC-UCL
Keywordsi
Molecular function | Transferase |
Biological process | DNA damage, DNA repair, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-2173788, Downregulation of TGF-beta receptor signaling R-MMU-5675482, Regulation of necroptotic cell death R-MMU-8863795, Downregulation of ERBB2 signaling R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase CHIPCurated (EC:2.3.2.272 Publications)Alternative name(s): Carboxy terminus of Hsp70-interacting protein1 Publication RING-type E3 ubiquitin transferase CHIPCurated STIP1 homology and U box-containing protein 1Curated |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1891731, Stub1 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Nucleus
- nuclear inclusion body Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- chaperone complex Source: MGI
- cytoplasm Source: ARUK-UCL
- ubiquitin conjugating enzyme complex Source: HGNC-UCL
- ubiquitin ligase complex Source: UniProtKB
- Z disc Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | K → R: Impaired interaction with ATXN3; when associated with R-4 and R-7. 1 Publication | 1 | |
Mutagenesisi | 4 | K → R: Impaired interaction with ATXN3; when associated with R-2 and R-7. 1 Publication | 1 | |
Mutagenesisi | 7 | K → R: Impaired interaction with ATXN3; when associated with R-2 and R-4. 1 Publication | 1 | |
Mutagenesisi | 261 | H → A: Loss of E3 ubiquitin protein ligase activity. 1 Publication | 1 | |
Mutagenesisi | 270 | P → A: Loss of E3 ubiquitin protein ligase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106330 | 1 – 304 | E3 ubiquitin-protein ligase CHIPAdd BLAST | 304 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 2 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Cross-linki | 23 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 24 | PhosphoserineCombined sources | 1 | |
Modified residuei | 26 | PhosphoserineCombined sources | 1 | |
Modified residuei | 150 | PhosphoserineBy similarity | 1 | |
Cross-linki | 222 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 256 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 274 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9WUD1 |
jPOSTi | Q9WUD1 |
MaxQBi | Q9WUD1 |
PaxDbi | Q9WUD1 |
PeptideAtlasi | Q9WUD1 |
PRIDEi | Q9WUD1 |
ProteomicsDBi | 283905 |
PTM databases
iPTMneti | Q9WUD1 |
PhosphoSitePlusi | Q9WUD1 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000039615, Expressed in lens of camera-type eye and 71 other tissues |
ExpressionAtlasi | Q9WUD1, baseline and differential |
Genevisiblei | Q9WUD1, MM |
Interactioni
Subunit structurei
Homodimer (PubMed:16307917).
Interacts with BAG2, and with the E2 ubiquitin conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1.
Interacts with MKKS.
Interacts with DNAAF4 and POLB (By similarity).
Interacts (via the U-box domain) with the UBE2V2-UBE2N heterodimer; the complex has a specific 'Lys-63'-linked polyubiquitination activity (PubMed:16307917).
Interacts (when monoubiquitinated) with ATXN3 (PubMed:21855799).
Interacts with UBE2W (PubMed:21855799).
Interacts with DNAJB6 (By similarity).
Interacts with FOXP3 (PubMed:23973223).
Interacts with FLCN and HSP90AA1.
Interacts with HSP90.
Interacts with UBE2N and UBE2V1.
Interacts (via TPR repeats) with the C-terminal domains of HSPA8 and HSPA1A.
Interacts with the non-acetylated form of HSPA1A and HSPA1B.
Interacts with SMAD3 and HSP90AB1 (By similarity).
Interacts with UBE4B (By similarity).
Interacts with RIPK3 (By similarity).
Interacts with PRMT5 (By similarity).
By similarity3 PublicationsBinary interactionsi
Hide detailsQ9WUD1
With | #Exp. | IntAct |
---|---|---|
Ilk [O55222] | 7 | EBI-773027,EBI-6690138 |
LRRK2 [Q5S007] from Homo sapiens. | 2 | EBI-773027,EBI-5323863 |
UBE2N [P61088] from Homo sapiens. | 2 | EBI-773027,EBI-1052908 |
GO - Molecular functioni
- chaperone binding Source: MGI
- enzyme binding Source: MGI
- G protein-coupled receptor binding Source: MGI
- heat shock protein binding Source: MGI
- Hsp70 protein binding Source: HGNC-UCL
- Hsp90 protein binding Source: BHF-UCL
- kinase binding Source: MGI
- misfolded protein binding Source: MGI
- protein homodimerization activity Source: HGNC-UCL
- SMAD binding Source: HGNC-UCL
- TPR domain binding Source: HGNC-UCL
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 207969, 68 interactors |
DIPi | DIP-29751N |
IntActi | Q9WUD1, 16 interactors |
MINTi | Q9WUD1 |
STRINGi | 10090.ENSMUSP00000040431 |
Miscellaneous databases
RNActi | Q9WUD1, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q9WUD1 |
SMRi | Q9WUD1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9WUD1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 27 – 60 | TPR 1Add BLAST | 34 | |
Repeati | 61 – 94 | TPR 2Add BLAST | 34 | |
Repeati | 96 – 128 | TPR 3Add BLAST | 33 | |
Domaini | 227 – 301 | U-boxAdd BLAST | 75 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 19 | Poly-Gly | 6 |
Domaini
Keywords - Domaini
Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG4642, Eukaryota |
GeneTreei | ENSGT00930000151045 |
HOGENOMi | CLU_056455_1_0_1 |
InParanoidi | Q9WUD1 |
OMAi | EVPDWAI |
OrthoDBi | 1422450at2759 |
PhylomeDBi | Q9WUD1 |
TreeFami | TF313937 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR041312, CHIP_TPR_N IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat IPR003613, Ubox_domain IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF18391, CHIP_TPR_N, 1 hit PF04564, U-box, 1 hit |
SMARTi | View protein in SMART SM00028, TPR, 3 hits SM00504, Ubox, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50005, TPR, 3 hits PS50293, TPR_REGION, 1 hit PS51698, U_BOX, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKGKEEKEGG ARLGTGGGGS PDKSPSAQEL KEQGNRLFVG RKYPEAAACY
60 70 80 90 100
GRAITRNPLV AVYYTNRALC YLKMQQPEQA LADCRRALEL DGQSVKAHFF
110 120 130 140 150
LGQCQLEMES YDEAIANLQR AYSLAKEQRL NFGDDIPSAL RIAKKKRWNS
160 170 180 190 200
IEERRIHQES ELHSYLTRLI AAERERELEE CQRNHEGHED DGHIRAQQAC
210 220 230 240 250
IEAKHDKYMA DMDELFSQVD EKRKKRDIPD YLCGKISFEL MREPCITPSG
260 270 280 290 300
ITYDRKDIEE HLQRVGHFDP VTRSPLTQEQ LIPNLAMKEV IDAFISENGW
VEDY
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A494BBI6 | A0A494BBI6_MOUSE | STIP1 homology and U box-containing... | Stub1 | 277 | Annotation score: | ||
A0A494BBF6 | A0A494BBF6_MOUSE | STIP1 homology and U box-containing... | Stub1 | 268 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 20 | S → T in BAB22329 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF129086 mRNA Translation: AAD33401.1 AK002752 mRNA Translation: BAB22329.1 AK004464 mRNA Translation: BAB23315.1 AK045776 mRNA Translation: BAC32489.1 AK166630 mRNA Translation: BAE38905.1 BC027427 mRNA Translation: AAH27427.1 BC038939 mRNA Translation: AAH38939.1 |
CCDSi | CCDS28533.1 |
RefSeqi | NP_062693.1, NM_019719.3 |
Genome annotation databases
Ensembli | ENSMUST00000044911; ENSMUSP00000040431; ENSMUSG00000039615 |
GeneIDi | 56424 |
KEGGi | mmu:56424 |
UCSCi | uc008bcf.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF129086 mRNA Translation: AAD33401.1 AK002752 mRNA Translation: BAB22329.1 AK004464 mRNA Translation: BAB23315.1 AK045776 mRNA Translation: BAC32489.1 AK166630 mRNA Translation: BAE38905.1 BC027427 mRNA Translation: AAH27427.1 BC038939 mRNA Translation: AAH38939.1 |
CCDSi | CCDS28533.1 |
RefSeqi | NP_062693.1, NM_019719.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2C2L | X-ray | 3.30 | A/B/C/D | 24-304 | [»] | |
2C2V | X-ray | 2.90 | S/T/U/V | 227-304 | [»] | |
3Q47 | X-ray | 1.70 | B | 23-155 | [»] | |
3Q49 | X-ray | 1.54 | B | 23-155 | [»] | |
3Q4A | X-ray | 1.54 | B | 23-155 | [»] | |
BMRBi | Q9WUD1 | |||||
SMRi | Q9WUD1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 207969, 68 interactors |
DIPi | DIP-29751N |
IntActi | Q9WUD1, 16 interactors |
MINTi | Q9WUD1 |
STRINGi | 10090.ENSMUSP00000040431 |
PTM databases
iPTMneti | Q9WUD1 |
PhosphoSitePlusi | Q9WUD1 |
Proteomic databases
EPDi | Q9WUD1 |
jPOSTi | Q9WUD1 |
MaxQBi | Q9WUD1 |
PaxDbi | Q9WUD1 |
PeptideAtlasi | Q9WUD1 |
PRIDEi | Q9WUD1 |
ProteomicsDBi | 283905 |
Protocols and materials databases
Antibodypediai | 22808, 471 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044911; ENSMUSP00000040431; ENSMUSG00000039615 |
GeneIDi | 56424 |
KEGGi | mmu:56424 |
UCSCi | uc008bcf.1, mouse |
Organism-specific databases
CTDi | 10273 |
MGIi | MGI:1891731, Stub1 |
Phylogenomic databases
eggNOGi | KOG4642, Eukaryota |
GeneTreei | ENSGT00930000151045 |
HOGENOMi | CLU_056455_1_0_1 |
InParanoidi | Q9WUD1 |
OMAi | EVPDWAI |
OrthoDBi | 1422450at2759 |
PhylomeDBi | Q9WUD1 |
TreeFami | TF313937 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-2173788, Downregulation of TGF-beta receptor signaling R-MMU-5675482, Regulation of necroptotic cell death R-MMU-8863795, Downregulation of ERBB2 signaling R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 56424, 17 hits in 57 CRISPR screens |
ChiTaRSi | Stub1, mouse |
EvolutionaryTracei | Q9WUD1 |
PROi | PR:Q9WUD1 |
RNActi | Q9WUD1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039615, Expressed in lens of camera-type eye and 71 other tissues |
ExpressionAtlasi | Q9WUD1, baseline and differential |
Genevisiblei | Q9WUD1, MM |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR041312, CHIP_TPR_N IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat IPR003613, Ubox_domain IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF18391, CHIP_TPR_N, 1 hit PF04564, U-box, 1 hit |
SMARTi | View protein in SMART SM00028, TPR, 3 hits SM00504, Ubox, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50005, TPR, 3 hits PS50293, TPR_REGION, 1 hit PS51698, U_BOX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHIP_MOUSE | |
Accessioni | Q9WUD1Primary (citable) accession number: Q9WUD1 Secondary accession number(s): Q9DCJ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | November 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 186 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references