Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chloride channel protein ClC-Ka

Gene

Clcnka

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. May be the basolateral chloride channel mediating net chloride absorption in CTAL cells.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi259CalciumBy similarity1
Metal bindingi261CalciumBy similarity1
Metal bindingi278CalciumBy similarity1
Metal bindingi281CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • regulation of body fluid levels Source: MGI
  • regulation of ion transmembrane transport Source: UniProtKB-KW
  • renal inner medulla development Source: MGI

Keywordsi

Molecular functionChloride channel, Ion channel, Voltage-gated channel
Biological processIon transport, Transport
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein ClC-Ka
Short name:
Chloride channel Ka
Alternative name(s):
ClC-K1
Gene namesi
Name:Clcnka
Synonyms:Clcnk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1329026 Clcnka

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
Transmembranei396 – 416HelicalSequence analysisAdd BLAST21
Transmembranei417 – 437HelicalSequence analysisAdd BLAST21
Transmembranei458 – 478HelicalSequence analysisAdd BLAST21
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21
Topological domaini507 – 687CytoplasmicSequence analysisAdd BLAST181

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944561 – 687Chloride channel protein ClC-KaAdd BLAST687

Proteomic databases

PaxDbiQ9WUB7
PRIDEiQ9WUB7

PTM databases

PhosphoSitePlusiQ9WUB7

Expressioni

Tissue specificityi

Specifically expressed in the kidney. All nephron segments expressing BSND also express CLCNK proreins.2 Publications

Gene expression databases

BgeeiENSMUSG00000033770 Expressed in 91 organ(s), highest expression level in stria vascularis of cochlear duct
CleanExiMM_CLCNKA
ExpressionAtlasiQ9WUB7 baseline and differential
GenevisibleiQ9WUB7 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with BSND. Forms heteromers with BSND in the thin ascending limb of Henle.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048520

Structurei

3D structure databases

ProteinModelPortaliQ9WUB7
SMRiQ9WUB7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 609CBS 1PROSITE-ProRule annotationAdd BLAST59
Domaini626 – 687CBS 2PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476 Eukaryota
COG0038 LUCA
GeneTreeiENSGT00760000119109
HOGENOMiHOG000231297
HOVERGENiHBG005332
InParanoidiQ9WUB7
KOiK05017
OMAiLAFEMTG
OrthoDBiEOG091G01RJ
TreeFamiTF300522

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
InterProiView protein in InterPro
IPR000644 CBS_dom
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
IPR002250 Cl_channel-K
PfamiView protein in Pfam
PF00571 CBS, 1 hit
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01119 CLCHANNELKDY
SMARTiView protein in SMART
SM00116 CBS, 1 hit
SUPFAMiSSF81340 SSF81340, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9WUB7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEELVGLREG SSGKPVTLQE LWGPCPRIRR GIRGGLEWLK ERLFRAREDW
60 70 80 90 100
YFLVALGVLM ALISYAMNFA IGRVVRAHKW LYREVGDGHL LRYLSWTVYP
110 120 130 140 150
VALLSFSSGF SQSITPFSGG SGLPELKTML SGVVLENYLD IKNFGAKVVG
160 170 180 190 200
LSCTLATGST IFLGKVGPFV HLSVMISAYL GRVRAKAVGD TESKAKEVEM
210 220 230 240 250
LSAAAAVGVA TVFAAPFSGV LFSIEVMSSH FSVWNYWRGF FAATCGAFMF
260 270 280 290 300
RLLAVFNSEQ ETITSIYKTR FRVDVPFDLP EIFFFVALGF ICGILSCVYL
310 320 330 340 350
FCQRNFLRFI KTNRYTSKLL ATSKPSYAAL VALVLASVTY PPGVGRFMAS
360 370 380 390 400
RLSMAEHLHS LFDNNSWALM TRNASPPWPS EPDPQNLWFE WYHPRFTIFG
410 420 430 440 450
TLAFFLIMKF WMLILATTIP MPAGYFMPIF IIGAAIGRLL GEALSVAFPE
460 470 480 490 500
GIVAGGEVYP IMPGGYALAG AAAFSGAVTH TISTALLAFE LTGQIVHALP
510 520 530 540 550
VLMAVLAANV ISQNLQPSFY DGTIMAKKLP YLPWIRGRQI GSYPVTVEHF
560 570 580 590 600
MNSNLTTLAK DMPLEEVVKV VTSTDVSQYP LVETRESQTL VGIVERTHLV
610 620 630 640 650
QALQTQPASW APGQERFLQD ILAGGCPTQP VTLQLSPETS LYQAHRLFEL
660 670 680
LTLQALFVTS RGRAVGSVSW VELKKAISTL INPPAPK
Length:687
Mass (Da):75,684
Last modified:July 27, 2011 - v2
Checksum:iA04ADFFC220224FA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2ADB4A2ADB4_MOUSE
Chloride channel protein ClC-Ka
Clcnka
192Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti372R → W in AAH37077 (PubMed:15489334).Curated1
Sequence conflicti380S → A in AAD21083 (PubMed:11014860).Curated1
Sequence conflicti380S → A in AAH37077 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124848 mRNA Translation: AAD21083.1
AY373832 mRNA Translation: AAQ81628.1
AL670285 Genomic DNA No translation available.
BC037077 mRNA Translation: AAH37077.1
CCDSiCCDS18871.1
RefSeqiNP_001139779.1, NM_001146307.1
NP_077723.3, NM_024412.3
UniGeneiMm.167900

Genome annotation databases

EnsembliENSMUST00000042617; ENSMUSP00000048520; ENSMUSG00000033770
ENSMUST00000105790; ENSMUSP00000101416; ENSMUSG00000033770
GeneIDi12733
KEGGimmu:12733
UCSCiuc008voe.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124848 mRNA Translation: AAD21083.1
AY373832 mRNA Translation: AAQ81628.1
AL670285 Genomic DNA No translation available.
BC037077 mRNA Translation: AAH37077.1
CCDSiCCDS18871.1
RefSeqiNP_001139779.1, NM_001146307.1
NP_077723.3, NM_024412.3
UniGeneiMm.167900

3D structure databases

ProteinModelPortaliQ9WUB7
SMRiQ9WUB7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048520

PTM databases

PhosphoSitePlusiQ9WUB7

Proteomic databases

PaxDbiQ9WUB7
PRIDEiQ9WUB7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042617; ENSMUSP00000048520; ENSMUSG00000033770
ENSMUST00000105790; ENSMUSP00000101416; ENSMUSG00000033770
GeneIDi12733
KEGGimmu:12733
UCSCiuc008voe.2 mouse

Organism-specific databases

CTDi1187
MGIiMGI:1329026 Clcnka

Phylogenomic databases

eggNOGiKOG0476 Eukaryota
COG0038 LUCA
GeneTreeiENSGT00760000119109
HOGENOMiHOG000231297
HOVERGENiHBG005332
InParanoidiQ9WUB7
KOiK05017
OMAiLAFEMTG
OrthoDBiEOG091G01RJ
TreeFamiTF300522

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Miscellaneous databases

PROiPR:Q9WUB7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033770 Expressed in 91 organ(s), highest expression level in stria vascularis of cochlear duct
CleanExiMM_CLCNKA
ExpressionAtlasiQ9WUB7 baseline and differential
GenevisibleiQ9WUB7 MM

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
InterProiView protein in InterPro
IPR000644 CBS_dom
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
IPR002250 Cl_channel-K
PfamiView protein in Pfam
PF00571 CBS, 1 hit
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01119 CLCHANNELKDY
SMARTiView protein in SMART
SM00116 CBS, 1 hit
SUPFAMiSSF81340 SSF81340, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCLCKA_MOUSE
AccessioniPrimary (citable) accession number: Q9WUB7
Secondary accession number(s): A2ADB3, Q6UB69, Q8JZU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again