UniProtKB - Q9WU78 (PDC6I_MOUSE)
Programmed cell death 6-interacting protein
Pdcd6ip
Functioni
GO - Molecular functioni
- calcium-dependent protein binding Source: MGI
- identical protein binding Source: IntAct
- proteinase activated receptor binding Source: MGI
- protein dimerization activity Source: UniProtKB
- protein homodimerization activity Source: MGI
- SH3 domain binding Source: MGI
GO - Biological processi
- actomyosin contractile ring assembly Source: UniProtKB
- apoptotic process Source: MGI
- bicellular tight junction assembly Source: UniProtKB
- maintenance of epithelial cell apical/basal polarity Source: UniProtKB
- midbody abscission Source: MGI
- mitotic cytokinesis Source: UniProtKB
- positive regulation of exosomal secretion Source: MGI
- positive regulation of extracellular exosome assembly Source: MGI
- protein homooligomerization Source: MGI
- protein transport Source: UniProtKB-KW
- regulation of centrosome duplication Source: MGI
- regulation of extracellular exosome assembly Source: MGI
- regulation of membrane permeability Source: UniProtKB
- ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway Source: MGI
- viral budding Source: MGI
- viral budding via host ESCRT complex Source: MGI
Keywordsi
Biological process | Apoptosis, Cell cycle, Cell division, Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-5213460, RIPK1-mediated regulated necrosis R-MMU-5675482, Regulation of necroptotic cell death |
Names & Taxonomyi
Protein namesi | Recommended name: Programmed cell death 6-interacting proteinAlternative name(s): ALG-2-interacting protein 1 ALG-2-interacting protein X E2F1-inducible protein Eig2 |
Gene namesi | Name:Pdcd6ip Synonyms:Aip1, Alix |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1333753, Pdcd6ip |
Subcellular locationi
Cytosol
- cytosol By similarity
Extracellular region or secreted
- extracellular exosome 1 Publication
Cytoskeleton
- centrosome By similarity
Other locations
- Melanosome By similarity
- tight junction 1 Publication
- Midbody ring By similarity
Note: Colocalized with CEP55 in the midbody during cytokinesis and at centrosomes in non-dividing cells (By similarity). Component of the actomyosin-tight junction complex (PubMed:27336173).By similarity1 Publication
Cytoskeleton
- actomyosin Source: UniProtKB
- microtubule organizing center Source: UniProtKB-SubCell
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum exit site Source: MGI
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- immunological synapse Source: MGI
Other locations
- bicellular tight junction Source: UniProtKB-SubCell
- cytoplasm Source: MGI
- Flemming body Source: UniProtKB
- intracellular membrane-bounded organelle Source: MGI
- melanosome Source: UniProtKB-SubCell
- myelin sheath Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cytoplasm, Cytoskeleton, Secreted, Tight junctionPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000218892 | 2 – 869 | Programmed cell death 6-interacting proteinAdd BLAST | 868 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 215 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 479 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 481 | PhosphoserineBy similarity | 1 | |
Modified residuei | 730 | PhosphoserineBy similarity | 1 | |
Modified residuei | 741 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 745 | Omega-N-methylarginineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q9WU78 |
jPOSTi | Q9WU78 |
PaxDbi | Q9WU78 |
PeptideAtlasi | Q9WU78 |
PRIDEi | Q9WU78 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9WU78 |
UCD-2DPAGEi | Q9WU78 |
PTM databases
iPTMneti | Q9WU78 |
PhosphoSitePlusi | Q9WU78 |
SwissPalmi | Q9WU78 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000032504, Expressed in embryonic post-anal tail and 312 other tissues |
Genevisiblei | Q9WU78, MM |
Interactioni
Subunit structurei
Self-associates (By similarity).
Interacts with SH3KBP1 (By similarity).
Interacts with PDCD6 in a calcium-dependent manner (By similarity).
Interacts with TSG101 in a calcium-dependent manner; PDCD6IP homooligomerization may be required for TSG101-binding (By similarity).
Interacts with SGSM3 (By similarity). Directly interacts with CHMP4A, CHMP4B and CHMP4C (By similarity). Directly interacts with CEP55 in a 1:2 stoechiometry; this interaction is required for PDCD6IP targeting to the midbody (By similarity). May interact with PDGFRB (By similarity).
Interacts with SH3GL1 and SH3GL2/endophilin-1 (By similarity).
Forms a complex with SDCBP and SDC2 (PubMed:22660413).
Found in a complex with F-actin, TJP1/ZO-1 and PARD3 (PubMed:27336173).
Interacts with CD2AP (By similarity).
Interacts with ARRDC1 (By similarity).
By similarity2 PublicationsBinary interactionsi
Hide detailsQ9WU78
With | #Exp. | IntAct |
---|---|---|
Plcg2 [Q8CIH5] | 7 | EBI-641897,EBI-617954 |
Sdcbp [O08992] | 3 | EBI-641897,EBI-538265 |
Pdcd6ip - isoform 1 [Q9WU78-1]
GO - Molecular functioni
- calcium-dependent protein binding Source: MGI
- identical protein binding Source: IntAct
- proteinase activated receptor binding Source: MGI
- protein dimerization activity Source: UniProtKB
- protein homodimerization activity Source: MGI
- SH3 domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202072, 10 interactors |
ComplexPortali | CPX-3283, Syndecan-1-syntenin-1-ALIX complex |
DIPi | DIP-41418N |
ELMi | Q9WU78 |
IntActi | Q9WU78, 17 interactors |
MINTi | Q9WU78 |
STRINGi | 10090.ENSMUSP00000107492 |
Miscellaneous databases
RNActi | Q9WU78, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 392 | BRO1PROSITE-ProRule annotationAdd BLAST | 390 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 176 – 503 | Interaction with CHMP4A, CHMP4B and CHMP4CBy similarityAdd BLAST | 328 | |
Regioni | 383 – 869 | Interaction with SDCBP1 PublicationAdd BLAST | 487 | |
Regioni | 503 – 869 | Self-associationBy similarityAdd BLAST | 367 | |
Regioni | 717 – 720 | Interaction with TSG101By similarity | 4 | |
Regioni | 798 – 807 | Interaction with CEP55By similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 717 – 861 | Pro-richAdd BLAST | 145 |
Phylogenomic databases
eggNOGi | KOG2220, Eukaryota |
GeneTreei | ENSGT01010000223048 |
HOGENOMi | CLU_007181_2_0_1 |
InParanoidi | Q9WU78 |
OMAi | TKYDQHP |
OrthoDBi | 550620at2759 |
TreeFami | TF323502 |
Family and domain databases
Gene3Di | 1.25.40.280, 1 hit |
InterProi | View protein in InterPro IPR025304, ALIX_V_dom IPR004328, BRO1_dom IPR038499, BRO1_sf |
Pfami | View protein in Pfam PF13949, ALIX_LYPXL_bnd, 1 hit PF03097, BRO1, 1 hit |
SMARTi | View protein in SMART SM01041, BRO1, 1 hit |
PROSITEi | View protein in PROSITE PS51180, BRO1, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR
60 70 80 90 100
RSALGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD
110 120 130 140 150
KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA
160 170 180 190 200
KQYQFASGAF LHIKDTVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF
210 220 230 240 250
LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPTLAAKQC
260 270 280 290 300
IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKNVASR YDEYVNVKDF
310 320 330 340 350
SDKINRALTA AKKDNDFIYH DRVPDLKDLD PIGKATLVKP TPVNVPVSQK
360 370 380 390 400
FTDLFEKMVP VSVQQSLAVF SQRKADLVNR SIAQMREATT LANGVLASLN
410 420 430 440 450
LPAAIEDVSG DTVPQSILTK STSVVEQGGI QTVDQLIKEL PELLQRNREI
460 470 480 490 500
LEESLRLLDE EEATDNDLRA KFKDRWQRTP SNDLYKPLRA EGAKFRAVLD
510 520 530 540 550
KAVQADGQVK ERYQSHRDTI ALLCKPEPEL NAAIPSANPA KTMQGSEVVS
560 570 580 590 600
VLKSLLSNLD EIKKERESLE NDLKSVNFDM TSKFLTALAQ DGVINEEALS
610 620 630 640 650
VTELDRIYGG LTSKVQESLK KQEGLLKNIQ VSHQEFSKMK QSNNEANLRE
660 670 680 690 700
EVLKNLATAY DNFVELVANL KEGTKFYNEL TEILVRFQNK CSDIVFARKT
710 720 730 740 750
ERDELLKDLQ QSIAREPSAP SIPPPAYQSS PAAGHAAAPP TPAPRTMPPA
760 770 780 790 800
KPQPPARPPP PVLPANRVPP ASAAAAPAGV GTASAAPPQT PGSAPPPQAQ
810 820 830 840 850
GPPYPTYPGY PGYCQMPMPM GYNPYAYGQY NMPYPPVYHQ SPGQAPYPGP
860
QQPTYPFPQP PQQSYYPQQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 329 – 333 | LDPIG → SGSYR in AAD26813 (PubMed:9880530).Curated | 5 | |
Sequence conflicti | 530 | L → V in CAA06329 (PubMed:10200558).Curated | 1 | |
Sequence conflicti | 547 – 548 | EV → DL in AAD26813 (PubMed:9880530).Curated | 2 | |
Sequence conflicti | 595 | N → T in AAH26823 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 625 | L → V in AAD26813 (PubMed:9880530).Curated | 1 | |
Sequence conflicti | 640 – 641 | KQ → NE in AAD26813 (PubMed:9880530).Curated | 2 | |
Sequence conflicti | 821 | G → R in AAD26813 (PubMed:9880530).Curated | 1 | |
Sequence conflicti | 853 | P → L in CAA06330 (PubMed:10200558).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007502 | 159 – 805 | Missing in isoform 2. 1 PublicationAdd BLAST | 647 | |
Alternative sequenceiVSP_007501 | 239 | K → KYFYFQ in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005073 mRNA Translation: CAA06329.1 AJ005074 mRNA Translation: CAA06330.1 AF119955 mRNA Translation: AAD26813.1 AK031256 mRNA Translation: BAC27323.1 AK167574 mRNA Translation: BAE39637.1 AK169704 mRNA Translation: BAE41316.1 AC162177 Genomic DNA No translation available. AC167246 Genomic DNA No translation available. CT025751 Genomic DNA No translation available. CH466621 Genomic DNA Translation: EDL08950.1 BC002261 mRNA Translation: AAH02261.1 BC026823 mRNA Translation: AAH26823.1 AF176514 mRNA Translation: AAD53115.1 |
CCDSi | CCDS23588.1 [Q9WU78-1] CCDS52947.1 [Q9WU78-3] |
RefSeqi | NP_001158149.1, NM_001164677.1 [Q9WU78-3] NP_001158150.1, NM_001164678.1 NP_035182.2, NM_011052.2 [Q9WU78-1] |
Genome annotation databases
Ensembli | ENSMUST00000035086; ENSMUSP00000035086; ENSMUSG00000032504 [Q9WU78-1] ENSMUST00000111861; ENSMUSP00000107492; ENSMUSG00000032504 [Q9WU78-3] |
GeneIDi | 18571 |
KEGGi | mmu:18571 |
UCSCi | uc009rwn.2, mouse [Q9WU78-1] uc009rwo.2, mouse [Q9WU78-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005073 mRNA Translation: CAA06329.1 AJ005074 mRNA Translation: CAA06330.1 AF119955 mRNA Translation: AAD26813.1 AK031256 mRNA Translation: BAC27323.1 AK167574 mRNA Translation: BAE39637.1 AK169704 mRNA Translation: BAE41316.1 AC162177 Genomic DNA No translation available. AC167246 Genomic DNA No translation available. CT025751 Genomic DNA No translation available. CH466621 Genomic DNA Translation: EDL08950.1 BC002261 mRNA Translation: AAH02261.1 BC026823 mRNA Translation: AAH26823.1 AF176514 mRNA Translation: AAD53115.1 |
CCDSi | CCDS23588.1 [Q9WU78-1] CCDS52947.1 [Q9WU78-3] |
RefSeqi | NP_001158149.1, NM_001164677.1 [Q9WU78-3] NP_001158150.1, NM_001164678.1 NP_035182.2, NM_011052.2 [Q9WU78-1] |
3D structure databases
SMRi | Q9WU78 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202072, 10 interactors |
ComplexPortali | CPX-3283, Syndecan-1-syntenin-1-ALIX complex |
DIPi | DIP-41418N |
ELMi | Q9WU78 |
IntActi | Q9WU78, 17 interactors |
MINTi | Q9WU78 |
STRINGi | 10090.ENSMUSP00000107492 |
PTM databases
iPTMneti | Q9WU78 |
PhosphoSitePlusi | Q9WU78 |
SwissPalmi | Q9WU78 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9WU78 |
UCD-2DPAGEi | Q9WU78 |
Proteomic databases
EPDi | Q9WU78 |
jPOSTi | Q9WU78 |
PaxDbi | Q9WU78 |
PeptideAtlasi | Q9WU78 |
PRIDEi | Q9WU78 |
Genome annotation databases
Ensembli | ENSMUST00000035086; ENSMUSP00000035086; ENSMUSG00000032504 [Q9WU78-1] ENSMUST00000111861; ENSMUSP00000107492; ENSMUSG00000032504 [Q9WU78-3] |
GeneIDi | 18571 |
KEGGi | mmu:18571 |
UCSCi | uc009rwn.2, mouse [Q9WU78-1] uc009rwo.2, mouse [Q9WU78-3] |
Organism-specific databases
CTDi | 10015 |
MGIi | MGI:1333753, Pdcd6ip |
Phylogenomic databases
eggNOGi | KOG2220, Eukaryota |
GeneTreei | ENSGT01010000223048 |
HOGENOMi | CLU_007181_2_0_1 |
InParanoidi | Q9WU78 |
OMAi | TKYDQHP |
OrthoDBi | 550620at2759 |
TreeFami | TF323502 |
Enzyme and pathway databases
Reactomei | R-MMU-5213460, RIPK1-mediated regulated necrosis R-MMU-5675482, Regulation of necroptotic cell death |
Miscellaneous databases
BioGRID-ORCSi | 18571, 4 hits in 17 CRISPR screens |
ChiTaRSi | Pdcd6ip, mouse |
PROi | PR:Q9WU78 |
RNActi | Q9WU78, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032504, Expressed in embryonic post-anal tail and 312 other tissues |
Genevisiblei | Q9WU78, MM |
Family and domain databases
Gene3Di | 1.25.40.280, 1 hit |
InterProi | View protein in InterPro IPR025304, ALIX_V_dom IPR004328, BRO1_dom IPR038499, BRO1_sf |
Pfami | View protein in Pfam PF13949, ALIX_LYPXL_bnd, 1 hit PF03097, BRO1, 1 hit |
SMARTi | View protein in SMART SM01041, BRO1, 1 hit |
PROSITEi | View protein in PROSITE PS51180, BRO1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDC6I_MOUSE | |
Accessioni | Q9WU78Primary (citable) accession number: Q9WU78 Secondary accession number(s): O88695 Q9QZN8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 23, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot