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UniProtKB - Q9WU78 (PDC6I_MOUSE)

Protein

Programmed cell death 6-interacting protein

Gene

Pdcd6ip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (By similarity). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (PubMed:27336173).By similarity1 Publication

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processApoptosis, Cell cycle, Cell division, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 6-interacting protein
Alternative name(s):
ALG-2-interacting protein 1
ALG-2-interacting protein X
E2F1-inducible protein
Eig2
Gene namesi
Name:Pdcd6ip
Synonyms:Aip1, Alix
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1333753 Pdcd6ip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Secreted, Tight junction

Pathology & Biotechi

Disruption phenotypei

Deficient mice are viable and have a normal lifespan, although they are smaller in size. Mutant mice develop progressive and severe bilateral hydrocephalus (PubMed:27336173).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002188922 – 869Programmed cell death 6-interacting proteinAdd BLAST868

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei215N6-acetyllysineBy similarity1
Modified residuei479PhosphothreonineBy similarity1
Modified residuei481PhosphoserineBy similarity1
Modified residuei730PhosphoserineBy similarity1
Modified residuei741PhosphothreonineBy similarity1
Modified residuei745Omega-N-methylarginineBy similarity1

Post-translational modificationi

May be phosphorylated on tyrosine residues by activated PDGFRB.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9WU78
PaxDbiQ9WU78
PeptideAtlasiQ9WU78
PRIDEiQ9WU78

2D gel databases

REPRODUCTION-2DPAGEiQ9WU78
UCD-2DPAGEiQ9WU78

PTM databases

iPTMnetiQ9WU78
PhosphoSitePlusiQ9WU78
SwissPalmiQ9WU78

Expressioni

Tissue specificityi

Ubiquitously expressed (PubMed:27336173). High expression in choroid plexus and low expression in cerebral cortex (at protein level) (PubMed:27336173).1 Publication

Gene expression databases

BgeeiENSMUSG00000032504 Expressed in 296 organ(s), highest expression level in fibroblast
CleanExiMM_PDCD6IP
GenevisibleiQ9WU78 MM

Interactioni

Subunit structurei

Self-associates (By similarity). Interacts with SH3KBP1 (By similarity). Interacts with PDCD6 in a calcium-dependent manner (By similarity). Interacts with TSG101 in a calcium-dependent manner; PDCD6IP homooligomerization may be required for TSG101-binding (By similarity). Interacts with SGSM3 (By similarity). Directly interacts with CHMP4A, CHMP4B and CHMP4C (By similarity). Directly interacts with CEP55 in a 1:2 stoechiometry; this interaction is required for PDCD6IP targeting to the midbody (By similarity). May interact with PDGFRB (By similarity). Interacts with SH3GL1 and SH3GL2/endophilin-1 (By similarity). Forms a complex with SDCBP and SDC2 (PubMed:22660413). Found in a complex with F-actin, TJP1/ZO-1 and PARD3 (PubMed:27336173). Interacts with CD2AP (By similarity). Interacts with ARRDC1 (By similarity).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi202072, 5 interactors
ComplexPortaliCPX-3283 Syndecan-1-syntenin-1-ALIX complex
DIPiDIP-41418N
ELMiQ9WU78
IntActiQ9WU78, 17 interactors
MINTiQ9WU78
STRINGi10090.ENSMUSP00000107492

Structurei

3D structure databases

ProteinModelPortaliQ9WU78
SMRiQ9WU78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 392BRO1PROSITE-ProRule annotationAdd BLAST390

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 503Interaction with CHMP4A, CHMP4B and CHMP4CBy similarityAdd BLAST328
Regioni383 – 869Interaction with SDCBP1 PublicationAdd BLAST487
Regioni503 – 869Self-associationBy similarityAdd BLAST367
Regioni717 – 720Interaction with TSG101By similarity4
Regioni798 – 807Interaction with CEP55By similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi717 – 861Pro-richAdd BLAST145

Phylogenomic databases

eggNOGiKOG2220 Eukaryota
ENOG410XQX6 LUCA
GeneTreeiENSGT00780000121909
HOVERGENiHBG053533
InParanoidiQ9WU78
KOiK12200
OMAiRNTAIWK
OrthoDBiEOG091G04NC
TreeFamiTF323502

Family and domain databases

Gene3Di1.25.40.280, 1 hit
InterProiView protein in InterPro
IPR025304 ALIX_V_dom
IPR004328 BRO1_dom
IPR038499 BRO1_sf
PfamiView protein in Pfam
PF13949 ALIX_LYPXL_bnd, 1 hit
PF03097 BRO1, 1 hit
SMARTiView protein in SMART
SM01041 BRO1, 1 hit
PROSITEiView protein in PROSITE
PS51180 BRO1, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9WU78-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR 
        60         70         80         90        100
RSALGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD 
       110        120        130        140        150
KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA 
       160        170        180        190        200
KQYQFASGAF LHIKDTVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF 
       210        220        230        240        250
LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPTLAAKQC 
       260        270        280        290        300
IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKNVASR YDEYVNVKDF 
       310        320        330        340        350
SDKINRALTA AKKDNDFIYH DRVPDLKDLD PIGKATLVKP TPVNVPVSQK 
       360        370        380        390        400
FTDLFEKMVP VSVQQSLAVF SQRKADLVNR SIAQMREATT LANGVLASLN 
       410        420        430        440        450
LPAAIEDVSG DTVPQSILTK STSVVEQGGI QTVDQLIKEL PELLQRNREI 
       460        470        480        490        500
LEESLRLLDE EEATDNDLRA KFKDRWQRTP SNDLYKPLRA EGAKFRAVLD 
       510        520        530        540        550
KAVQADGQVK ERYQSHRDTI ALLCKPEPEL NAAIPSANPA KTMQGSEVVS 
       560        570        580        590        600
VLKSLLSNLD EIKKERESLE NDLKSVNFDM TSKFLTALAQ DGVINEEALS 
       610        620        630        640        650
VTELDRIYGG LTSKVQESLK KQEGLLKNIQ VSHQEFSKMK QSNNEANLRE 
       660        670        680        690        700
EVLKNLATAY DNFVELVANL KEGTKFYNEL TEILVRFQNK CSDIVFARKT 
       710        720        730        740        750
ERDELLKDLQ QSIAREPSAP SIPPPAYQSS PAAGHAAAPP TPAPRTMPPA 
       760        770        780        790        800
KPQPPARPPP PVLPANRVPP ASAAAAPAGV GTASAAPPQT PGSAPPPQAQ 
       810        820        830        840        850
GPPYPTYPGY PGYCQMPMPM GYNPYAYGQY NMPYPPVYHQ SPGQAPYPGP 
       860 
QQPTYPFPQP PQQSYYPQQ
Length:869
Mass (Da):96,024
Last modified:July 27, 2011 - v3
Checksum:iBFDC87ACA183C5D4
GO
Isoform 2 (identifier: Q9WU78-2) [UniParc]FASTAAdd to basket
Also known as: Alix-SF, Short

The sequence of this isoform differs from the canonical sequence as follows:
     159-805: Missing.

Note: Does not interact with ALG-2.
Show »
Length:222
Mass (Da):24,924
Checksum:i35BEBFF783AF1519
GO
Isoform 3 (identifier: Q9WU78-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-239: K → KYFYFQ

Show »
Length:874
Mass (Da):96,773
Checksum:i4B3AA18F84E39723
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti329 – 333LDPIG → SGSYR in AAD26813 (PubMed:9880530).Curated5
Sequence conflicti530L → V in CAA06329 (PubMed:10200558).Curated1
Sequence conflicti547 – 548EV → DL in AAD26813 (PubMed:9880530).Curated2
Sequence conflicti595N → T in AAH26823 (PubMed:15489334).Curated1
Sequence conflicti625L → V in AAD26813 (PubMed:9880530).Curated1
Sequence conflicti640 – 641KQ → NE in AAD26813 (PubMed:9880530).Curated2
Sequence conflicti821G → R in AAD26813 (PubMed:9880530).Curated1
Sequence conflicti853P → L in CAA06330 (PubMed:10200558).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007502159 – 805Missing in isoform 2. 1 PublicationAdd BLAST647
Alternative sequenceiVSP_007501239K → KYFYFQ in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005073 mRNA Translation: CAA06329.1
AJ005074 mRNA Translation: CAA06330.1
AF119955 mRNA Translation: AAD26813.1
AK031256 mRNA Translation: BAC27323.1
AK167574 mRNA Translation: BAE39637.1
AK169704 mRNA Translation: BAE41316.1
CT025751, AC162177, AC167246 Genomic DNA Translation: CAX15748.1
CH466621 Genomic DNA Translation: EDL08950.1
BC002261 mRNA Translation: AAH02261.1
BC026823 mRNA Translation: AAH26823.1
AF176514 mRNA Translation: AAD53115.1
CCDSiCCDS23588.1 [Q9WU78-1]
CCDS52947.1 [Q9WU78-3]
RefSeqiNP_001158149.1, NM_001164677.1 [Q9WU78-3]
NP_001158150.1, NM_001164678.1
NP_035182.2, NM_011052.2 [Q9WU78-1]
UniGeneiMm.29816

Genome annotation databases

EnsembliENSMUST00000035086; ENSMUSP00000035086; ENSMUSG00000032504 [Q9WU78-1]
ENSMUST00000111861; ENSMUSP00000107492; ENSMUSG00000032504 [Q9WU78-3]
GeneIDi18571
KEGGimmu:18571
UCSCiuc009rwn.2 mouse [Q9WU78-1]
uc009rwo.2 mouse [Q9WU78-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005073 mRNA Translation: CAA06329.1
AJ005074 mRNA Translation: CAA06330.1
AF119955 mRNA Translation: AAD26813.1
AK031256 mRNA Translation: BAC27323.1
AK167574 mRNA Translation: BAE39637.1
AK169704 mRNA Translation: BAE41316.1
CT025751, AC162177, AC167246 Genomic DNA Translation: CAX15748.1
CH466621 Genomic DNA Translation: EDL08950.1
BC002261 mRNA Translation: AAH02261.1
BC026823 mRNA Translation: AAH26823.1
AF176514 mRNA Translation: AAD53115.1
CCDSiCCDS23588.1 [Q9WU78-1]
CCDS52947.1 [Q9WU78-3]
RefSeqiNP_001158149.1, NM_001164677.1 [Q9WU78-3]
NP_001158150.1, NM_001164678.1
NP_035182.2, NM_011052.2 [Q9WU78-1]
UniGeneiMm.29816

3D structure databases

ProteinModelPortaliQ9WU78
SMRiQ9WU78
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202072, 5 interactors
ComplexPortaliCPX-3283 Syndecan-1-syntenin-1-ALIX complex
DIPiDIP-41418N
ELMiQ9WU78
IntActiQ9WU78, 17 interactors
MINTiQ9WU78
STRINGi10090.ENSMUSP00000107492

PTM databases

iPTMnetiQ9WU78
PhosphoSitePlusiQ9WU78
SwissPalmiQ9WU78

2D gel databases

REPRODUCTION-2DPAGEiQ9WU78
UCD-2DPAGEiQ9WU78

Proteomic databases

EPDiQ9WU78
PaxDbiQ9WU78
PeptideAtlasiQ9WU78
PRIDEiQ9WU78

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035086; ENSMUSP00000035086; ENSMUSG00000032504 [Q9WU78-1]
ENSMUST00000111861; ENSMUSP00000107492; ENSMUSG00000032504 [Q9WU78-3]
GeneIDi18571
KEGGimmu:18571
UCSCiuc009rwn.2 mouse [Q9WU78-1]
uc009rwo.2 mouse [Q9WU78-3]

Organism-specific databases

CTDi10015
MGIiMGI:1333753 Pdcd6ip

Phylogenomic databases

eggNOGiKOG2220 Eukaryota
ENOG410XQX6 LUCA
GeneTreeiENSGT00780000121909
HOVERGENiHBG053533
InParanoidiQ9WU78
KOiK12200
OMAiRNTAIWK
OrthoDBiEOG091G04NC
TreeFamiTF323502

Miscellaneous databases

ChiTaRSiPdcd6ip mouse
PROiPR:Q9WU78
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032504 Expressed in 296 organ(s), highest expression level in fibroblast
CleanExiMM_PDCD6IP
GenevisibleiQ9WU78 MM

Family and domain databases

Gene3Di1.25.40.280, 1 hit
InterProiView protein in InterPro
IPR025304 ALIX_V_dom
IPR004328 BRO1_dom
IPR038499 BRO1_sf
PfamiView protein in Pfam
PF13949 ALIX_LYPXL_bnd, 1 hit
PF03097 BRO1, 1 hit
SMARTiView protein in SMART
SM01041 BRO1, 1 hit
PROSITEiView protein in PROSITE
PS51180 BRO1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPDC6I_MOUSE
AccessioniPrimary (citable) accession number: Q9WU78
Secondary accession number(s): O88695
, O89014, Q3TED2, Q8BSL8, Q8R0H5, Q99LR3, Q9QZN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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