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Protein

Amiloride-sensitive sodium channel subunit beta

Gene

Scnn1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.1 Publication

Enzyme regulationi

Activated by WNK1, WNK2, WNK3 and WNK4.1 Publication

GO - Molecular functioni

  • ligand-gated sodium channel activity Source: MGI
  • WW domain binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionIon channel, Sodium channel
Biological processIon transport, Sensory transduction, Sodium transport, Taste, Transport
LigandSodium

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Names & Taxonomyi

Protein namesi
Recommended name:
Amiloride-sensitive sodium channel subunit beta
Alternative name(s):
Beta-NaCH
Epithelial Na(+) channel subunit beta
Short name:
Beta-ENaC
Nonvoltage-gated sodium channel 1 subunit beta
SCNEB
Gene namesi
Name:Scnn1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104696 Scnn1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 50CytoplasmicBy similarityAdd BLAST50
Transmembranei51 – 71Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini72 – 530ExtracellularBy similarityAdd BLAST459
Transmembranei531 – 551Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini552 – 638CytoplasmicBy similarityAdd BLAST87

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001812691 – 638Amiloride-sensitive sodium channel subunit betaAdd BLAST638

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi135N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi205N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei631PhosphoserineCombined sources1
Modified residuei633PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated. N-glycosylation is required for interaction with BPIFA1.By similarity
Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9WU38
PaxDbiQ9WU38
PRIDEiQ9WU38

PTM databases

iPTMnetiQ9WU38
PhosphoSitePlusiQ9WU38
SwissPalmiQ9WU38

Expressioni

Tissue specificityi

Lung and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000030873
CleanExiMM_SCNN1B
ExpressionAtlasiQ9WU38 baseline and differential
GenevisibleiQ9WU38 MM

Interactioni

Subunit structurei

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties (By similarity). Interacts with NEDD4 (via WW domains) (PubMed:11244092, PubMed:15123669). Interacts with NEDD4L (via WW domains) (PubMed:11244092, PubMed:12424229, PubMed:15123669). Interacts with WWP1 (via WW domains). Interacts with WWP2 (via WW domains). Interacts with the full-length immature form of PCSK9 (pro-PCSK9) (By similarity). Interacts (N-glycosylated) with BPIFA1; the interaction is direct and inhibits the proteolytic processing of SCNN1A and SCNN1G and the activation of ENaC (By similarity).By similarity1 Publication3 Publications

GO - Molecular functioni

  • WW domain binding Source: MGI

Protein-protein interaction databases

BioGridi203106, 5 interactors
ComplexPortaliCPX-315 Amiloride-sensitive sodium channel complex, alpha-beta-gamma
DIPiDIP-40891N
IntActiQ9WU38, 3 interactors
STRINGi10090.ENSMUSP00000033161

Structurei

3D structure databases

ProteinModelPortaliQ9WU38
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4294 Eukaryota
ENOG410ZNFK LUCA
GeneTreeiENSGT00760000119120
HOGENOMiHOG000236286
HOVERGENiHBG058435
InParanoidiQ9WU38
KOiK04825
OMAiCNDTQYK
OrthoDBiEOG091G0KW6
PhylomeDBiQ9WU38
TreeFamiTF330663

Family and domain databases

InterProiView protein in InterPro
IPR001873 ENaC
IPR004724 ENaC_chordates
IPR020903 ENaC_CS
PANTHERiPTHR11690 PTHR11690, 1 hit
PfamiView protein in Pfam
PF00858 ASC, 1 hit
PRINTSiPR01078 AMINACHANNEL
TIGRFAMsiTIGR00859 ENaC, 1 hit
PROSITEiView protein in PROSITE
PS01206 ASC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WU38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVKKYLLKC LHRLQKGPGY TYKELLVWYC NNTNTHGPKR IICEGPKKKA
60 70 80 90 100
MWFLLTLLFA CLVCWQWGVF IQTYLSWEVS VSLSMGFKTM NFPAVTVCNS
110 120 130 140 150
SPFQYSKVKH LLKDLDELME AVLEKILAPE ASHSNTTRTL NFTIWNHTPL
160 170 180 190 200
VLIDERNPDH PVVLNLFGDS HNSSNPAPGS TCNAQGCKVA MRLCSANGTV
210 220 230 240 250
CTLRNFTSAT QAVTEWYILQ ATNIFSQVLP QDLVGMGYAP DRIILACLFG
260 270 280 290 300
TEPCSHRNFT PIFYPDYGNC YIFNWGMTEE TLPSANPGTE FGLKLILDIG
310 320 330 340 350
QEDYVPFLAS TAGARLMLHE QRTYPFIREE GIYAMAGTET SIGVLVDKLQ
360 370 380 390 400
RKGEPYSPCT MNGSDVAIKN LYSVYNTTYS IQACLHSCFQ DHMIRNCSCG
410 420 430 440 450
HYLYPLPEGE KYCNNRDFPD WAYCYLNLQM SVTQRETCLS MCKESCNDTQ
460 470 480 490 500
YKMTISMADW PSEASEDWIL HVLSQERDQS SNITLSRKGI VKLNIYFQEF
510 520 530 540 550
NYRTIEESPA NNIVWLLSNL GGQFGFWMGG SVLCLIEFGE IIIDFIWITI
560 570 580 590 600
IKLVASCKGL RRRRPQAPYT GPPPTVAELV EAHTNFGFQP DTTSCRPHGE
610 620 630
VYPDQQTLPI PGTPPPNYDS LRLQPLDTME SDSEVEAI
Length:638
Mass (Da):72,197
Last modified:November 1, 1999 - v1
Checksum:iF502A510BFE65392
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174S → N in BAC29591 (PubMed:16141072).Curated1
Sequence conflicti239A → T in BAC26190 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112186 mRNA Translation: AAD21245.1
AK028909 mRNA Translation: BAC26190.1
AK036819 mRNA Translation: BAC29591.1
CCDSiCCDS21804.1
RefSeqiNP_001258952.1, NM_001272023.1
NP_035455.1, NM_011325.2
UniGeneiMm.7709

Genome annotation databases

EnsembliENSMUST00000033161; ENSMUSP00000033161; ENSMUSG00000030873
GeneIDi20277
KEGGimmu:20277
UCSCiuc009jnx.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSCNNB_MOUSE
AccessioniPrimary (citable) accession number: Q9WU38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: June 20, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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