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Protein

Methylenetetrahydrofolate reductase

Gene

Mthfr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.By similarity

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.By similarity

Cofactori

FADBy similarity

Activity regulationi

Allosterically regulated by S-adenosylmethionine.By similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei62Proton donor/acceptorBy similarity1
Binding sitei126FADBy similarity1
Binding sitei158SubstrateBy similarity1
Binding sitei196FADBy similarity1
Binding sitei209FADBy similarity1
Binding sitei216FADBy similarity1
Binding sitei227SubstrateBy similarity1
Binding sitei320SubstrateBy similarity1
Binding sitei324SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 67NADBy similarity6
Nucleotide bindingi93 – 94NAD and FADBy similarity2
Nucleotide bindingi156 – 158FADBy similarity3
Nucleotide bindingi173 – 174FADBy similarity2
Nucleotide bindingi200 – 203FADBy similarity4

GO - Molecular functioni

  • FAD binding Source: GO_Central
  • flavin adenine dinucleotide binding Source: MGI
  • methylenetetrahydrofolate reductase (NAD(P)H) activity Source: MGI
  • modified amino acid binding Source: MGI
  • NADP binding Source: MGI
  • protein-containing complex binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.5.1.15 3474
1.5.1.20 3474
ReactomeiR-MMU-196757 Metabolism of folate and pterines
UniPathwayi
UPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductaseCurated (EC:1.5.1.20By similarity)
Gene namesi
Name:Mthfr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:106639 Mthfr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902471 – 654Methylenetetrahydrofolate reductaseAdd BLAST654

Proteomic databases

EPDiQ9WU20
MaxQBiQ9WU20
PaxDbiQ9WU20
PRIDEiQ9WU20

PTM databases

iPTMnetiQ9WU20
PhosphoSitePlusiQ9WU20

Expressioni

Gene expression databases

BgeeiENSMUSG00000029009 Expressed in 275 organ(s), highest expression level in ear vesicle
CleanExiMM_MTHFR
ExpressionAtlasiQ9WU20 baseline and differential
GenevisibleiQ9WU20 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069774

Structurei

3D structure databases

ProteinModelPortaliQ9WU20
SMRiQ9WU20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0564 Eukaryota
COG0685 LUCA
GeneTreeiENSGT00390000012490
HOGENOMiHOG000246234
HOVERGENiHBG006414
InParanoidiQ9WU20
KOiK00297

Family and domain databases

CDDicd00537 MTHFR, 1 hit
InterProiView protein in InterPro
IPR029041 FAD-linked_oxidoreductase-like
IPR004621 Fadh2_euk
IPR003171 Mehydrof_redctse
PfamiView protein in Pfam
PF02219 MTHFR, 1 hit
SUPFAMiSSF51730 SSF51730, 1 hit
TIGRFAMsiTIGR00677 fadh2_euk, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q9WU20-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVNEARGSGS PNPRSEGSSS GSESSKDSSR CSTPSLDPER HERLREKMRR
60 70 80 90 100
RMDSGDKWFS LEFFPPRTAE GAVNLISRFD RMAAGGPLFV DVTWHPAGDP
110 120 130 140 150
GSDKETSSMM IASTAVNYCG LETILHMTCC QQRPEEITGH LHRAKQLGLK
160 170 180 190 200
NIMALRGDPV GDHWEAEEGG FSYATDLVKH IRTEFADYFD ICVAGYPRGH
210 220 230 240 250
PDAESFEDDL KHLKEKVSAG ADFIITQLFF EASTFFSFVK ACTEIGISCP
260 270 280 290 300
ILPGIFPIQG YTSLRQLVKL SKLEVPQKIK DVIEPIKDND AAIRNYGIEL
310 320 330 340 350
AVSLCRELLD SGLVPGLHFY TLNREVATME VLKQLGMWTE DPRRPLPWAL
360 370 380 390 400
SAHPKRREED VRPIFWASRP KSYIYRTQDW DEFPNGRWGN SSSPAFGELK
410 420 430 440 450
DYYLFYLKSK SPREELLKMW GEELTSEESV FEVFEHYLSG EPNRHGYRVT
460 470 480 490 500
CLPWNDEPLA AETSLMKEEL LRVNRLGILT INSQPNINAK PSSDPVVGWG
510 520 530 540 550
PSGGYVFQKA YLEFFTSRET VEALLQVLKT YELRVNYHIV DVKGENITNA
560 570 580 590 600
PELQPNAVTW GIFPGREIIQ PTVVDPISFM FWKDEAFALW IEQWGKLYEE
610 620 630 640 650
ESPSRMIIQY IHDNYFLVNL VDNEFPLDSC LWQVVEDTFE LLNRHPTERE

TQAP
Length:654
Mass (Da):74,580
Last modified:July 27, 2011 - v2
Checksum:i96E625014D8F4838
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A7F7A2A7F7_MOUSE
Methylenetetrahydrofolate reductase
Mthfr mCG_11725
695Annotation score:
F6Z4F8F6Z4F8_MOUSE
Methylenetetrahydrofolate reductase
Mthfr
670Annotation score:
A2A7F9A2A7F9_MOUSE
Methylenetetrahydrofolate reductase
Mthfr
194Annotation score:
F7BGN3F7BGN3_MOUSE
Methylenetetrahydrofolate reductase
Mthfr
152Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti303S → R in AAD20313 (PubMed:9680386).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105998
, AF105988, AF105989, AF105990, AF105991, AF105992, AF105993, AF105994, AF105995, AF105996, AF105997 Genomic DNA Translation: AAD20313.1
AK030192 mRNA Translation: BAC26832.1
AL606929 Genomic DNA Translation: CAM14896.1
CH466594 Genomic DNA Translation: EDL14794.1
CH466594 Genomic DNA Translation: EDL14796.1
CH466594 Genomic DNA Translation: EDL14797.1
BC051017 mRNA Translation: AAH51017.1
BC052466 mRNA Translation: AAH52466.1
CCDSiCCDS18929.1
RefSeqiNP_034970.2, NM_010840.3
UniGeneiMm.89959

Genome annotation databases

EnsembliENSMUST00000097788; ENSMUSP00000095395; ENSMUSG00000029009
GeneIDi17769
KEGGimmu:17769
UCSCiuc008vtu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105998
, AF105988, AF105989, AF105990, AF105991, AF105992, AF105993, AF105994, AF105995, AF105996, AF105997 Genomic DNA Translation: AAD20313.1
AK030192 mRNA Translation: BAC26832.1
AL606929 Genomic DNA Translation: CAM14896.1
CH466594 Genomic DNA Translation: EDL14794.1
CH466594 Genomic DNA Translation: EDL14796.1
CH466594 Genomic DNA Translation: EDL14797.1
BC051017 mRNA Translation: AAH51017.1
BC052466 mRNA Translation: AAH52466.1
CCDSiCCDS18929.1
RefSeqiNP_034970.2, NM_010840.3
UniGeneiMm.89959

3D structure databases

ProteinModelPortaliQ9WU20
SMRiQ9WU20
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069774

PTM databases

iPTMnetiQ9WU20
PhosphoSitePlusiQ9WU20

Proteomic databases

EPDiQ9WU20
MaxQBiQ9WU20
PaxDbiQ9WU20
PRIDEiQ9WU20

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097788; ENSMUSP00000095395; ENSMUSG00000029009
GeneIDi17769
KEGGimmu:17769
UCSCiuc008vtu.2 mouse

Organism-specific databases

CTDi4524
MGIiMGI:106639 Mthfr

Phylogenomic databases

eggNOGiKOG0564 Eukaryota
COG0685 LUCA
GeneTreeiENSGT00390000012490
HOGENOMiHOG000246234
HOVERGENiHBG006414
InParanoidiQ9WU20
KOiK00297

Enzyme and pathway databases

UniPathwayi
UPA00193

BRENDAi1.5.1.15 3474
1.5.1.20 3474
ReactomeiR-MMU-196757 Metabolism of folate and pterines

Miscellaneous databases

ChiTaRSiMthfr mouse
PROiPR:Q9WU20
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029009 Expressed in 275 organ(s), highest expression level in ear vesicle
CleanExiMM_MTHFR
ExpressionAtlasiQ9WU20 baseline and differential
GenevisibleiQ9WU20 MM

Family and domain databases

CDDicd00537 MTHFR, 1 hit
InterProiView protein in InterPro
IPR029041 FAD-linked_oxidoreductase-like
IPR004621 Fadh2_euk
IPR003171 Mehydrof_redctse
PfamiView protein in Pfam
PF02219 MTHFR, 1 hit
SUPFAMiSSF51730 SSF51730, 1 hit
TIGRFAMsiTIGR00677 fadh2_euk, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTHR_MOUSE
AccessioniPrimary (citable) accession number: Q9WU20
Secondary accession number(s): Q8CDE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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