UniProtKB - Q9WU19 (HAOX1_MOUSE)
2-Hydroxyacid oxidase 1
Hao1
Functioni
Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed:9891009).
The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (By similarity).
Can also catalyze the oxidation glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate (By similarity).
Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed:9891009).
By similarity1 PublicationCatalytic activityi
- EC:1.1.3.151 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:1.1.3.151 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:1.2.3.5By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Cofactori
: glycine biosynthesis Pathwayi
This protein is involved in the pathway glycine biosynthesis, which is part of Amino-acid biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway glycine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 26 | GlyoxylateBy similarity | 1 | |
Binding sitei | 108 | FMNBy similarity | 1 | |
Binding sitei | 130 | FMNBy similarity | 1 | |
Binding sitei | 132 | GlyoxylateBy similarity | 1 | |
Binding sitei | 158 | FMNBy similarity | 1 | |
Binding sitei | 167 | GlyoxylateBy similarity | 1 | |
Binding sitei | 236 | FMNBy similarity | 1 | |
Binding sitei | 258 | FMNBy similarity | 1 | |
Active sitei | 260 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 260 | GlyoxylateBy similarity | 1 | |
Binding sitei | 263 | GlyoxylateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 79 – 81 | FMNBy similarity | 3 | |
Nucleotide bindingi | 291 – 295 | FMNBy similarity | 5 | |
Nucleotide bindingi | 314 – 315 | FMNBy similarity | 2 |
GO - Molecular functioni
- (S)-2-hydroxy-acid oxidase activity Source: MGI
- FMN binding Source: UniProtKB
- glyoxylate oxidase activity Source: Ensembl
GO - Biological processi
- fatty acid alpha-oxidation Source: UniProtKB
- glycolate catabolic process Source: UniProtKB
- response to oxidative stress Source: Ensembl
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
BRENDAi | 1.1.3.15, 3474 |
Reactomei | R-MMU-389661, Glyoxylate metabolism and glycine degradation R-MMU-9033241, Peroxisomal protein import |
UniPathwayi | UPA00288 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Hao1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96011, Hao1 |
VEuPathDBi | HostDB:ENSMUSG00000027261 |
Subcellular locationi
Peroxisome
- Peroxisome matrix By similarity
Peroxisome
- peroxisomal matrix Source: Ensembl
- peroxisome Source: HGNC-UCL
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206319 | 1 – 370 | 2-Hydroxyacid oxidase 1Add BLAST | 370 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 184 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 194 | PhosphoserineCombined sources | 1 | |
Modified residuei | 230 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9WU19 |
MaxQBi | Q9WU19 |
PaxDbi | Q9WU19 |
PRIDEi | Q9WU19 |
ProteomicsDBi | 269712 |
PTM databases
iPTMneti | Q9WU19 |
PhosphoSitePlusi | Q9WU19 |
SwissPalmi | Q9WU19 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000027261, Expressed in liver and 67 other tissues |
ExpressionAtlasi | Q9WU19, baseline and differential |
Genevisiblei | Q9WU19, MM |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
BioGRIDi | 200206, 2 interactors |
STRINGi | 10090.ENSMUSP00000028704 |
Chemistry databases
BindingDBi | Q9WU19 |
Miscellaneous databases
RNActi | Q9WU19, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 365 | FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST | 365 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 368 – 370 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
GeneTreei | ENSGT00390000018717 |
HOGENOMi | CLU_020639_6_1_1 |
InParanoidi | Q9WU19 |
OMAi | FTRLMQT |
OrthoDBi | 879633at2759 |
PhylomeDBi | Q9WU19 |
TreeFami | TF313363 |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLPRLVCISD YEQHVRSVLQ KSVYDYYRSG ANDQETLADN IQAFSRWKLY
60 70 80 90 100
PRMLRNVADI DLSTSVLGQR VSMPICVGAT AMQCMAHVDG ELATVRACQT
110 120 130 140 150
MGTGMMLSSW ATSSIEEVAE AGPEALRWMQ LYIYKDREIS RQIVKRAEKQ
160 170 180 190 200
GYKAIFVTVD TPYLGNRIDD VRNRFKLPPQ LRMKNFETND LAFSPKGNFG
210 220 230 240 250
DNSGLAEYVA QAIDPSLSWD DITWLRRLTS LPIVVKGILR GDDAKEAVKH
260 270 280 290 300
GVDGILVSNH GARQLDGVPA TIDVLPEIVE AVEGKVEVFL DGGVRKGTDV
310 320 330 340 350
LKALALGAKA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ
360 370
NVKVIDKTLV RKNPLAVSKI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104312 mRNA Translation: AAD25332.1 |
CCDSi | CCDS16783.1 |
RefSeqi | NP_034533.1, NM_010403.2 |
Genome annotation databases
Ensembli | ENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261 |
GeneIDi | 15112 |
KEGGi | mmu:15112 |
UCSCi | uc008mns.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104312 mRNA Translation: AAD25332.1 |
CCDSi | CCDS16783.1 |
RefSeqi | NP_034533.1, NM_010403.2 |
3D structure databases
SMRi | Q9WU19 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200206, 2 interactors |
STRINGi | 10090.ENSMUSP00000028704 |
Chemistry databases
BindingDBi | Q9WU19 |
ChEMBLi | CHEMBL4295985 |
PTM databases
iPTMneti | Q9WU19 |
PhosphoSitePlusi | Q9WU19 |
SwissPalmi | Q9WU19 |
Proteomic databases
jPOSTi | Q9WU19 |
MaxQBi | Q9WU19 |
PaxDbi | Q9WU19 |
PRIDEi | Q9WU19 |
ProteomicsDBi | 269712 |
Protocols and materials databases
Antibodypediai | 8448, 398 antibodies from 32 providers |
DNASUi | 15112 |
Genome annotation databases
Ensembli | ENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261 |
GeneIDi | 15112 |
KEGGi | mmu:15112 |
UCSCi | uc008mns.1, mouse |
Organism-specific databases
CTDi | 54363 |
MGIi | MGI:96011, Hao1 |
VEuPathDBi | HostDB:ENSMUSG00000027261 |
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
GeneTreei | ENSGT00390000018717 |
HOGENOMi | CLU_020639_6_1_1 |
InParanoidi | Q9WU19 |
OMAi | FTRLMQT |
OrthoDBi | 879633at2759 |
PhylomeDBi | Q9WU19 |
TreeFami | TF313363 |
Enzyme and pathway databases
UniPathwayi | UPA00288 |
BRENDAi | 1.1.3.15, 3474 |
Reactomei | R-MMU-389661, Glyoxylate metabolism and glycine degradation R-MMU-9033241, Peroxisomal protein import |
Miscellaneous databases
BioGRID-ORCSi | 15112, 2 hits in 65 CRISPR screens |
ChiTaRSi | Hao1, mouse |
PROi | PR:Q9WU19 |
RNActi | Q9WU19, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027261, Expressed in liver and 67 other tissues |
ExpressionAtlasi | Q9WU19, baseline and differential |
Genevisiblei | Q9WU19, MM |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAOX1_MOUSE | |
Accessioni | Q9WU19Primary (citable) accession number: Q9WU19 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families