UniProtKB - Q9WU19 (HAOX1_MOUSE)
Protein
Hydroxyacid oxidase 1
Gene
Hao1
Organism
Mus musculus (Mouse)
Status
Functioni
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate (By similarity).By similarity1 Publication
Catalytic activityi
- EC:1.1.3.15
Cofactori
: glycolate degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Hydroxyacid oxidase 1 (Hao1)
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 26 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 108 | FMNPROSITE-ProRule annotation | 1 | |
Binding sitei | 130 | FMNPROSITE-ProRule annotation | 1 | |
Binding sitei | 132 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 158 | FMNPROSITE-ProRule annotation | 1 | |
Binding sitei | 167 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 236 | FMNPROSITE-ProRule annotation | 1 | |
Active sitei | 260 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 263 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 315 | FMNPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 291 – 315 | FMNPROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- (S)-2-hydroxy-acid oxidase activity Source: MGI
- FMN binding Source: UniProtKB
- glycolate oxidase activity Source: UniProtKB
- long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
- medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
- very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
GO - Biological processi
- fatty acid alpha-oxidation Source: UniProtKB
- glycolate catabolic process Source: UniProtKB
- response to oxidative stress Source: Ensembl
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
Reactomei | R-MMU-389661, Glyoxylate metabolism and glycine degradation R-MMU-9033241, Peroxisomal protein import |
UniPathwayi | UPA00864;UER00830 |
Names & Taxonomyi
Protein namesi | Recommended name: Hydroxyacid oxidase 1 (EC:1.1.3.15)Short name: HAOX1 Alternative name(s): Glycolate oxidase Short name: GOX |
Gene namesi | Name:Hao1 Synonyms:Gox1, Hao-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96011, Hao1 |
Subcellular locationi
Peroxisome
Peroxisome
- peroxisome Source: HGNC-UCL
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206319 | 1 – 370 | Hydroxyacid oxidase 1Add BLAST | 370 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 184 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 194 | PhosphoserineCombined sources | 1 | |
Modified residuei | 230 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9WU19 |
MaxQBi | Q9WU19 |
PaxDbi | Q9WU19 |
PRIDEi | Q9WU19 |
PTM databases
iPTMneti | Q9WU19 |
PhosphoSitePlusi | Q9WU19 |
SwissPalmi | Q9WU19 |
Expressioni
Tissue specificityi
Liver.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000027261, Expressed in liver and 64 other tissues |
ExpressionAtlasi | Q9WU19, baseline and differential |
Genevisiblei | Q9WU19, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 200206, 2 interactors |
IntActi | Q9WU19, 2 interactors |
MINTi | Q9WU19 |
STRINGi | 10090.ENSMUSP00000028704 |
Miscellaneous databases
RNActi | Q9WU19, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 365 | FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST | 365 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 368 – 370 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
GeneTreei | ENSGT00390000018717 |
HOGENOMi | CLU_020639_6_1_1 |
InParanoidi | Q9WU19 |
OMAi | RIWFRPQ |
OrthoDBi | 879633at2759 |
PhylomeDBi | Q9WU19 |
TreeFami | TF313363 |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9WU19-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLPRLVCISD YEQHVRSVLQ KSVYDYYRSG ANDQETLADN IQAFSRWKLY
60 70 80 90 100
PRMLRNVADI DLSTSVLGQR VSMPICVGAT AMQCMAHVDG ELATVRACQT
110 120 130 140 150
MGTGMMLSSW ATSSIEEVAE AGPEALRWMQ LYIYKDREIS RQIVKRAEKQ
160 170 180 190 200
GYKAIFVTVD TPYLGNRIDD VRNRFKLPPQ LRMKNFETND LAFSPKGNFG
210 220 230 240 250
DNSGLAEYVA QAIDPSLSWD DITWLRRLTS LPIVVKGILR GDDAKEAVKH
260 270 280 290 300
GVDGILVSNH GARQLDGVPA TIDVLPEIVE AVEGKVEVFL DGGVRKGTDV
310 320 330 340 350
LKALALGAKA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ
360 370
NVKVIDKTLV RKNPLAVSKI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104312 mRNA Translation: AAD25332.1 |
CCDSi | CCDS16783.1 |
RefSeqi | NP_034533.1, NM_010403.2 |
Genome annotation databases
Ensembli | ENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261 |
GeneIDi | 15112 |
KEGGi | mmu:15112 |
UCSCi | uc008mns.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104312 mRNA Translation: AAD25332.1 |
CCDSi | CCDS16783.1 |
RefSeqi | NP_034533.1, NM_010403.2 |
3D structure databases
SMRi | Q9WU19 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200206, 2 interactors |
IntActi | Q9WU19, 2 interactors |
MINTi | Q9WU19 |
STRINGi | 10090.ENSMUSP00000028704 |
Chemistry databases
ChEMBLi | CHEMBL4295985 |
PTM databases
iPTMneti | Q9WU19 |
PhosphoSitePlusi | Q9WU19 |
SwissPalmi | Q9WU19 |
Proteomic databases
jPOSTi | Q9WU19 |
MaxQBi | Q9WU19 |
PaxDbi | Q9WU19 |
PRIDEi | Q9WU19 |
Protocols and materials databases
Antibodypediai | 8448, 379 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028704; ENSMUSP00000028704; ENSMUSG00000027261 |
GeneIDi | 15112 |
KEGGi | mmu:15112 |
UCSCi | uc008mns.1, mouse |
Organism-specific databases
CTDi | 54363 |
MGIi | MGI:96011, Hao1 |
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
GeneTreei | ENSGT00390000018717 |
HOGENOMi | CLU_020639_6_1_1 |
InParanoidi | Q9WU19 |
OMAi | RIWFRPQ |
OrthoDBi | 879633at2759 |
PhylomeDBi | Q9WU19 |
TreeFami | TF313363 |
Enzyme and pathway databases
UniPathwayi | UPA00864;UER00830 |
Reactomei | R-MMU-389661, Glyoxylate metabolism and glycine degradation R-MMU-9033241, Peroxisomal protein import |
Miscellaneous databases
BioGRID-ORCSi | 15112, 2 hits in 17 CRISPR screens |
ChiTaRSi | Hao1, mouse |
PROi | PR:Q9WU19 |
RNActi | Q9WU19, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027261, Expressed in liver and 64 other tissues |
ExpressionAtlasi | Q9WU19, baseline and differential |
Genevisiblei | Q9WU19, MM |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HAOX1_MOUSE | |
Accessioni | Q9WU19Primary (citable) accession number: Q9WU19 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families