UniProtKB - Q9WTX6 (CUL1_MOUSE)
Cullin-1
Cul1
Functioni
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- ubiquitin protein ligase binding Source: GO_Central
- ubiquitin-protein transferase activity Source: GO_Central
GO - Biological processi
- animal organ morphogenesis Source: MGI
- apoptotic process Source: MGI
- cell population proliferation Source: MGI
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein K48-linked ubiquitination Source: MGI
- protein monoubiquitination Source: MGI
- protein ubiquitination Source: MGI
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: GO_Central
- viral process Source: UniProtKB-KW
Keywordsi
Biological process | Host-virus interaction, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1170546, Prolactin receptor signaling R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5684264, MAP3K8 (TPL2)-dependent MAPK1/3 activation R-MMU-68949, Orc1 removal from chromatin R-MMU-69231, Cyclin D associated events in G1 R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-917937, Iron uptake and transport R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Cullin-1Short name: CUL-1 |
Gene namesi | Name:Cul1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1349658, Cul1 |
Subcellular locationi
Nucleus
- nucleoplasm Source: Reactome
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cullin-RING ubiquitin ligase complex Source: MGI
- Parkin-FBXW7-Cul1 ubiquitin ligase complex Source: MGI
- SCF ubiquitin ligase complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119788 | 1 – 776 | Cullin-1Add BLAST | 776 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 63 | Omega-N-methylarginineCombined sources | 1 | |
Cross-linki | 720 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Methylation, Ubl conjugationProteomic databases
EPDi | Q9WTX6 |
MaxQBi | Q9WTX6 |
PaxDbi | Q9WTX6 |
PeptideAtlasi | Q9WTX6 |
PRIDEi | Q9WTX6 |
PTM databases
iPTMneti | Q9WTX6 |
PhosphoSitePlusi | Q9WTX6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000029686, Expressed in embryo and 315 other tissues |
ExpressionAtlasi | Q9WTX6, baseline and differential |
Genevisiblei | Q9WTX6, MM |
Interactioni
Subunit structurei
Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes formed of CUL1, SKP1, RBX1 and a variable F-box domain-containing protein as substrate-specific subunit (PubMed:10097128, PubMed:12140560, PubMed:16880526, PubMed:23452855, PubMed:23452856).
Component of the SCF(FBXW11) complex containing FBXW11.
Component of the SCF(SKP2) complex containing SKP2, in which it interacts directly with SKP1, SKP2 and RBX1.
Component of the SCF(FBXW2) complex containing FBXW2.
Component of the SCF(FBXO32) complex containing FBXO32.
Component of the probable SCF(FBXO7) complex containing FBXO7.
Component of the SCF(FBXO10) complex containing FBXO10.
Component of the SCF(FBXO11) complex containing FBXO11.
Component of the SCF(FBXO25) complex containing FBXO25.
Component of the SCF(FBXO33) complex containing FBXO33.
Component of the probable SCF(FBXO4) complex containing FBXO4.
Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44 (By similarity).
Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC (PubMed:10097128, PubMed:11735228). This complex binds phosphorylated NFKBIA (PubMed:10097128).
Part of a SCF complex consisting of CUL1, RBX1, SKP1 and FBXO2.
Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2.
Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17.
Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27.
Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF.
Component of the SCF(FBXL3) complex composed of CUL1, SKP1, RBX1 and FBXL3.
Component of the SCF(FBXL21) complex composed of CUL1, SKP1, RBX1 and FBXL21.
Component of the SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9.
Component of the SCF(FBXW7) composed of CUL1, SKP1, RBX1 and FBXW7.
Interacts with CHEK2; mediates CHEK2 ubiquitination and regulates its function.
Part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction mediates the exchange of the F-box substrate-specific subunit. Can self-associate (By similarity).
Interacts with FBXW8 (PubMed:16880526).
Interacts with RNF7 (By similarity).
Interacts with CUL7; the interaction seems to be mediated by FBXW8 (PubMed:16880526).
Interacts with TRIM21 (By similarity).
Interacts with COPS2 (PubMed:11967155).
Interacts with DCUN1D1 and UBE2M.
Interacts with DCUN1D3.
Interacts with DCUN1D4 (By similarity).
Identified in a complex with RBX1 and GLMN (By similarity).
Interacts with CEP68 as part of the SCF(FBXW11) complex; the interaction is probably mediated by FBXW11 and the complex also contains CDK5RAP2 and PCNT.
Interacts (when neddylated) with ARIH1; leading to activate the E3 ligase activity of ARIH1.
Interacts with COPS9.
Interacts with UBXN1 (By similarity).
Interacts with KAT7, probably as part of an SCF complex; the interaction mediates KAT7 ubiquitination (PubMed:23319590).
Interacts with NOTCH2 (By similarity).
Part of a complex that contains DCUN1D5, CUL1 and RBX1; this interaction is bridged by CUL1 (By similarity).
Interacts (unneddylated form) with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5; these interactions promote the cullin neddylation (By similarity).
By similarity8 Publications(Microbial infection) Interacts with murine cytomegalovirus M48.
1 PublicationBinary interactionsi
Hide detailsQ9WTX6
With | #Exp. | IntAct |
---|---|---|
UBXN7 [O94888] from Homo sapiens. | 3 | EBI-1551052,EBI-1993627 |
VCP [P55072] from Homo sapiens. | 2 | EBI-1551052,EBI-355164 |
GO - Molecular functioni
- ubiquitin protein ligase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 205088, 98 interactors |
CORUMi | Q9WTX6 |
DIPi | DIP-39799N |
IntActi | Q9WTX6, 42 interactors |
MINTi | Q9WTX6 |
STRINGi | 10090.ENSMUSP00000031697 |
Miscellaneous databases
RNActi | Q9WTX6, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2166, Eukaryota |
GeneTreei | ENSGT00940000154774 |
HOGENOMi | CLU_004747_6_1_1 |
InParanoidi | Q9WTX6 |
OMAi | WLWHLCK |
OrthoDBi | 1040292at2759 |
PhylomeDBi | Q9WTX6 |
TreeFami | TF101151 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
InterProi | View protein in InterPro IPR016157, Cullin_CS IPR016158, Cullin_homology IPR036317, Cullin_homology_sf IPR001373, Cullin_N IPR019559, Cullin_neddylation_domain IPR016159, Cullin_repeat-like_dom_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00888, Cullin, 1 hit PF10557, Cullin_Nedd8, 1 hit |
SMARTi | View protein in SMART SM00182, CULLIN, 1 hit SM00884, Cullin_Nedd8, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF74788, SSF74788, 1 hit SSF75632, SSF75632, 1 hit |
PROSITEi | View protein in PROSITE PS01256, CULLIN_1, 1 hit PS50069, CULLIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSSNRSQNPH GLKQIGLDQI WDDLRAGIQQ VYTRQSMAKS RYMELYTHVY
60 70 80 90 100
NYCTSVHQSN QARGAGVPPS KSKKGQTPGG AQFVGLELYK RLKEFLKNYL
110 120 130 140 150
TNLLKDGEDL MDESVLKFYT QQWEDYRFSS KVLNGICAYL NRHWVRRECD
160 170 180 190 200
EGRKGIYEIY SLALVTWRDC LFRPLNKQVT NAVLKLIEKE RNGETINTRL
210 220 230 240 250
ISGVVQSYVE LGLNEDDAFA KGPTLTVYKE SFESQFLADT ERFYTRESTE
260 270 280 290 300
FLQQNPVTEY MKKAEARLLE EQRRVQVYLH ESTQDELARK CEQVLIEKHL
310 320 330 340 350
EIFHTEFQNL LDADKNEDLG RMYNLVSRIQ DGLGELKKLL ETHIHNQGLA
360 370 380 390 400
AIEKCGEAAL NDPKMYVQTV LDVHKKYNAL VMSAFNNDAG FVAALDKACG
410 420 430 440 450
RFINNNAVTK MAQSSSKSPE LLARYCDSLL KKSSKNPEEA ELEDTLNQVM
460 470 480 490 500
VVFKYIEDKD VFQKFYAKML AKRLVHQNSA SDDAEASMIS KLKQACGFEY
510 520 530 540 550
TSKLQRMFQD IGVSKDLNEQ FKKHLTNSEP LDLDFSIQVL SSGSWPFQQS
560 570 580 590 600
CTFALPSELE RSYQRFTAFY ASRHSGRKLT WLYQLSKGEL VTNCFKNRYT
610 620 630 640 650
LQASTFQMAI LLQYNTEDAY TVQQLTDSTQ IKMDILAQVL QILLKSKLLV
660 670 680 690 700
LEDENANVDE VELKPDTLIK LYLGYKNKKL RVNINVPMKT EQKQEQETTH
710 720 730 740 750
KNIEEDRKLL IQAAIVRIMK MRKVLKHQQL LGEVLTQLSS RFKPRVPVIK
760 770
KCIDILIEKE YLERVDGEKD TYSYLA
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TPM3 | Q3TPM3_MOUSE | Cullin-1 | Cul1 | 699 | Annotation score: | ||
D3Z2H3 | D3Z2H3_MOUSE | Cullin-1 | Cul1 | 110 | Annotation score: | ||
D3YUS8 | D3YUS8_MOUSE | Cullin-1 | Cul1 | 20 | Annotation score: | ||
A0A0N4SUW1 | A0A0N4SUW1_MOUSE | Cullin-1 | Cul1 | 51 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | K → R in AAD34471 (Ref. 3) Curated | 1 | |
Sequence conflicti | 249 | T → S in AAD34471 (Ref. 3) Curated | 1 | |
Sequence conflicti | 308 | Q → H in AAD34471 (Ref. 3) Curated | 1 | |
Sequence conflicti | 334 | G → R in AAD34471 (Ref. 3) Curated | 1 | |
Sequence conflicti | 640 – 663 | LQILL…DEVEL → YRFTEVEIAGLRDEMPMLMR WM in AAD34471 (Ref. 3) CuratedAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF083216 mRNA Translation: AAD16038.1 AF176910 mRNA Translation: AAD52657.1 AF136441 mRNA Translation: AAD34471.1 BC029260 mRNA Translation: AAH29260.1 |
CCDSi | CCDS20095.1 |
RefSeqi | NP_036172.1, NM_012042.3 XP_006506251.1, XM_006506188.3 XP_006506252.1, XM_006506189.3 XP_006506253.1, XM_006506190.3 |
Genome annotation databases
Ensembli | ENSMUST00000031697; ENSMUSP00000031697; ENSMUSG00000029686 |
GeneIDi | 26965 |
KEGGi | mmu:26965 |
UCSCi | uc009bsz.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF083216 mRNA Translation: AAD16038.1 AF176910 mRNA Translation: AAD52657.1 AF136441 mRNA Translation: AAD34471.1 BC029260 mRNA Translation: AAH29260.1 |
CCDSi | CCDS20095.1 |
RefSeqi | NP_036172.1, NM_012042.3 XP_006506251.1, XM_006506188.3 XP_006506252.1, XM_006506189.3 XP_006506253.1, XM_006506190.3 |
3D structure databases
SMRi | Q9WTX6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205088, 98 interactors |
CORUMi | Q9WTX6 |
DIPi | DIP-39799N |
IntActi | Q9WTX6, 42 interactors |
MINTi | Q9WTX6 |
STRINGi | 10090.ENSMUSP00000031697 |
PTM databases
iPTMneti | Q9WTX6 |
PhosphoSitePlusi | Q9WTX6 |
Proteomic databases
EPDi | Q9WTX6 |
MaxQBi | Q9WTX6 |
PaxDbi | Q9WTX6 |
PeptideAtlasi | Q9WTX6 |
PRIDEi | Q9WTX6 |
Protocols and materials databases
Antibodypediai | 3630, 525 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031697; ENSMUSP00000031697; ENSMUSG00000029686 |
GeneIDi | 26965 |
KEGGi | mmu:26965 |
UCSCi | uc009bsz.1, mouse |
Organism-specific databases
CTDi | 8454 |
MGIi | MGI:1349658, Cul1 |
Phylogenomic databases
eggNOGi | KOG2166, Eukaryota |
GeneTreei | ENSGT00940000154774 |
HOGENOMi | CLU_004747_6_1_1 |
InParanoidi | Q9WTX6 |
OMAi | WLWHLCK |
OrthoDBi | 1040292at2759 |
PhylomeDBi | Q9WTX6 |
TreeFami | TF101151 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1170546, Prolactin receptor signaling R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5684264, MAP3K8 (TPL2)-dependent MAPK1/3 activation R-MMU-68949, Orc1 removal from chromatin R-MMU-69231, Cyclin D associated events in G1 R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-917937, Iron uptake and transport R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 26965, 8 hits in 18 CRISPR screens |
ChiTaRSi | Cul1, mouse |
PROi | PR:Q9WTX6 |
RNActi | Q9WTX6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029686, Expressed in embryo and 315 other tissues |
ExpressionAtlasi | Q9WTX6, baseline and differential |
Genevisiblei | Q9WTX6, MM |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
InterProi | View protein in InterPro IPR016157, Cullin_CS IPR016158, Cullin_homology IPR036317, Cullin_homology_sf IPR001373, Cullin_N IPR019559, Cullin_neddylation_domain IPR016159, Cullin_repeat-like_dom_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00888, Cullin, 1 hit PF10557, Cullin_Nedd8, 1 hit |
SMARTi | View protein in SMART SM00182, CULLIN, 1 hit SM00884, Cullin_Nedd8, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF74788, SSF74788, 1 hit SSF75632, SSF75632, 1 hit |
PROSITEi | View protein in PROSITE PS01256, CULLIN_1, 1 hit PS50069, CULLIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CUL1_MOUSE | |
Accessioni | Q9WTX6Primary (citable) accession number: Q9WTX6 Secondary accession number(s): Q9WUI7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families