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Protein

S-phase kinase-associated protein 1

Gene

Skp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2 (By similarity).By similarity3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1170546 Prolactin receptor signaling
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5684264 MAP3K8 (TPL2)-dependent MAPK1/3 activation
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69231 Cyclin D associated events in G1
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8951664 Neddylation
R-MMU-9020702 Interleukin-1 signaling
R-MMU-917937 Iron uptake and transport
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
S-phase kinase-associated protein 1
Alternative name(s):
Cyclin-A/CDK2-associated protein p19
S-phase kinase-associated protein 1A
p19A
p19skp1
Gene namesi
Name:Skp1
Synonyms:Skp1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:103575 Skp1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872521 – 163S-phase kinase-associated protein 1Add BLAST163

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei131PhosphothreonineCombined sources1
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9WTX5
MaxQBiQ9WTX5
PaxDbiQ9WTX5
PRIDEiQ9WTX5

2D gel databases

REPRODUCTION-2DPAGEiQ9WTX5

PTM databases

iPTMnetiQ9WTX5
PhosphoSitePlusiQ9WTX5
SwissPalmiQ9WTX5

Expressioni

Gene expression databases

BgeeiENSMUSG00000036309 Expressed in 299 organ(s), highest expression level in cochlea
CleanExiMM_SKP1A
ExpressionAtlasiQ9WTX5 baseline and differential
GenevisibleiQ9WTX5 MM

Interactioni

Subunit structurei

Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes formed of CUL1, SKP1, RBX1 and a variable F-box domain-containing protein as substrate-specific subunit. Component of the SCF(FBXW11) complex containing FBXW11. Component of the SCF(SKP2) complex containing SKP2, in which it interacts directly with SKP1, SKP2 and RBX1. Component of the SCF(FBXW2) complex containing FBXw2. Component of the SCF(FBXO32) complex containing FBXO32. Component of the probable SCF(FBXO7) complex containing FBXO7. Component of the SCF(FBXO10) complex containing FBXO10. Component of the SCF(FBXO11) complex containing FBXO11. Component of the SCF(FBXO25) complex containing FBXO25. Component of the SCF(FBXO33) complex containing FBXO33. Component of the probable SCF(FBXO4) complex containing FBXO4. Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44. Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. This complex binds phosphorylated NFKBIA. Part of a SCF complex consisting of CUL1, RBX1, SKP1 and FBXO2. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27. Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Component of the SCF(FBXL3) complex composed of CUL1, SKP1, RBX1 and FBXL3. Component of the SCF(FBXL21) complex composed of CUL1, SKP1, RBX1 and FBXL21. Component of the SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9. Component of the SCF(FBXW7) composed of CUL1, SKP1, RBX1 and FBXW7. Component of the SCF(FBXW15) complex containing FBXW15 (PubMed:23319590). Interacts with CEP68 (By similarity). Interacts with FBXW15 (PubMed:23319590). Interacts with NOTCH2 (By similarity).By similarity6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203998, 73 interactors
CORUMiQ9WTX5
DIPiDIP-38596N
IntActiQ9WTX5, 54 interactors
MINTiQ9WTX5
STRINGi10090.ENSMUSP00000038744

Structurei

3D structure databases

ProteinModelPortaliQ9WTX5
SMRiQ9WTX5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 163Interaction with the F-box domain of F-box proteinsBy similarityAdd BLAST60

Sequence similaritiesi

Belongs to the SKP1 family.Curated

Phylogenomic databases

eggNOGiKOG1724 Eukaryota
COG5201 LUCA
GeneTreeiENSGT00390000012652
HOGENOMiHOG000172184
HOVERGENiHBG057008
InParanoidiQ9WTX5
KOiK03094
OMAiENKWCEE
OrthoDBiEOG091G0OU2
PhylomeDBiQ9WTX5
TreeFamiTF354233

Family and domain databases

InterProiView protein in InterPro
IPR016897 SKP1
IPR001232 SKP1-like
IPR036296 SKP1-like_dim_sf
IPR011333 SKP1/BTB/POZ_sf
IPR016072 Skp1_comp_dimer
IPR016073 Skp1_comp_POZ
PfamiView protein in Pfam
PF01466 Skp1, 1 hit
PF03931 Skp1_POZ, 1 hit
PIRSFiPIRSF028729 E3_ubiquit_lig_SCF_Skp, 1 hit
SMARTiView protein in SMART
SM00512 Skp1, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit
SSF81382 SSF81382, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9WTX5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPTIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV
60 70 80 90 100
NAAILKKVIQ WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL
110 120 130 140 150
FELILAANYL DIKGLLDVTC KTVANMIKGK TPEEIRKTFN IKNDFTEEEE
160
AQVRKENQWC EEK
Length:163
Mass (Da):18,672
Last modified:January 23, 2007 - v3
Checksum:iAE184C00BE6556DB
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PUV4E9PUV4_MOUSE
S-phase kinase-associated protein 1...
Skp1a
96Annotation score:
E9Q3Q7E9Q3Q7_MOUSE
S-phase kinase-associated protein 1...
Skp1a
56Annotation score:
F6TGP8F6TGP8_MOUSE
S-phase kinase-associated protein 1...
Skp1a
50Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41D → N in BAC37220 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083214 mRNA Translation: AAD16036.1
AK002983 mRNA Translation: BAB22496.1
AK010628 mRNA Translation: BAB27074.1
AK012498 mRNA Translation: BAB28281.1
AK014245 mRNA Translation: BAB29222.1
AK027909 mRNA Translation: BAC25660.1
AK078330 mRNA Translation: BAC37220.1
AK088339 mRNA Translation: BAC40292.1
BC002115 mRNA Translation: AAH02115.1
CCDSiCCDS24669.1
RefSeqiNP_035673.3, NM_011543.4
XP_006532849.1, XM_006532786.2
UniGeneiMm.42944

Genome annotation databases

EnsembliENSMUST00000037324; ENSMUSP00000038744; ENSMUSG00000036309
ENSMUST00000109072; ENSMUSP00000104700; ENSMUSG00000036309
GeneIDi21402
KEGGimmu:21402
UCSCiuc007ivf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083214 mRNA Translation: AAD16036.1
AK002983 mRNA Translation: BAB22496.1
AK010628 mRNA Translation: BAB27074.1
AK012498 mRNA Translation: BAB28281.1
AK014245 mRNA Translation: BAB29222.1
AK027909 mRNA Translation: BAC25660.1
AK078330 mRNA Translation: BAC37220.1
AK088339 mRNA Translation: BAC40292.1
BC002115 mRNA Translation: AAH02115.1
CCDSiCCDS24669.1
RefSeqiNP_035673.3, NM_011543.4
XP_006532849.1, XM_006532786.2
UniGeneiMm.42944

3D structure databases

ProteinModelPortaliQ9WTX5
SMRiQ9WTX5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203998, 73 interactors
CORUMiQ9WTX5
DIPiDIP-38596N
IntActiQ9WTX5, 54 interactors
MINTiQ9WTX5
STRINGi10090.ENSMUSP00000038744

PTM databases

iPTMnetiQ9WTX5
PhosphoSitePlusiQ9WTX5
SwissPalmiQ9WTX5

2D gel databases

REPRODUCTION-2DPAGEiQ9WTX5

Proteomic databases

EPDiQ9WTX5
MaxQBiQ9WTX5
PaxDbiQ9WTX5
PRIDEiQ9WTX5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037324; ENSMUSP00000038744; ENSMUSG00000036309
ENSMUST00000109072; ENSMUSP00000104700; ENSMUSG00000036309
GeneIDi21402
KEGGimmu:21402
UCSCiuc007ivf.2 mouse

Organism-specific databases

CTDi21402
MGIiMGI:103575 Skp1a

Phylogenomic databases

eggNOGiKOG1724 Eukaryota
COG5201 LUCA
GeneTreeiENSGT00390000012652
HOGENOMiHOG000172184
HOVERGENiHBG057008
InParanoidiQ9WTX5
KOiK03094
OMAiENKWCEE
OrthoDBiEOG091G0OU2
PhylomeDBiQ9WTX5
TreeFamiTF354233

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1170546 Prolactin receptor signaling
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5684264 MAP3K8 (TPL2)-dependent MAPK1/3 activation
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69231 Cyclin D associated events in G1
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8951664 Neddylation
R-MMU-9020702 Interleukin-1 signaling
R-MMU-917937 Iron uptake and transport
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiSkp1a mouse
PROiPR:Q9WTX5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036309 Expressed in 299 organ(s), highest expression level in cochlea
CleanExiMM_SKP1A
ExpressionAtlasiQ9WTX5 baseline and differential
GenevisibleiQ9WTX5 MM

Family and domain databases

InterProiView protein in InterPro
IPR016897 SKP1
IPR001232 SKP1-like
IPR036296 SKP1-like_dim_sf
IPR011333 SKP1/BTB/POZ_sf
IPR016072 Skp1_comp_dimer
IPR016073 Skp1_comp_POZ
PfamiView protein in Pfam
PF01466 Skp1, 1 hit
PF03931 Skp1_POZ, 1 hit
PIRSFiPIRSF028729 E3_ubiquit_lig_SCF_Skp, 1 hit
SMARTiView protein in SMART
SM00512 Skp1, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit
SSF81382 SSF81382, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSKP1_MOUSE
AccessioniPrimary (citable) accession number: Q9WTX5
Secondary accession number(s): Q8C5H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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