UniProtKB - Q9WTX5 (SKP1_MOUSE)
S-phase kinase-associated protein 1
Skp1
Functioni
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- beta-catenin binding Source: MGI
- cullin family protein binding Source: MGI
- F-box domain binding Source: MGI
- protein domain specific binding Source: MGI
- ubiquitin ligase-substrate adaptor activity Source: MGI
GO - Biological processi
- histone H2A monoubiquitination Source: MGI
- maintenance of protein location in nucleus Source: MGI
- positive regulation of ubiquitin protein ligase activity Source: MGI
- protein K48-linked ubiquitination Source: MGI
- protein polyubiquitination Source: MGI
- protein ubiquitination Source: MGI
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: MGI
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1170546, Prolactin receptor signaling R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5684264, MAP3K8 (TPL2)-dependent MAPK1/3 activation R-MMU-68949, Orc1 removal from chromatin R-MMU-69231, Cyclin D associated events in G1 R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-917937, Iron uptake and transport R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: S-phase kinase-associated protein 1Alternative name(s): Cyclin-A/CDK2-associated protein p19 S-phase kinase-associated protein 1A p19A p19skp1 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103575, Skp1 |
Subcellular locationi
Cytoskeleton
- centrosome Source: CACAO
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: MGI
- PcG protein complex Source: Ensembl
Other locations
- Cul7-RING ubiquitin ligase complex Source: UniProtKB
- cytoplasm Source: MGI
- SCF ubiquitin ligase complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000187252 | 1 – 163 | S-phase kinase-associated protein 1Add BLAST | 163 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 131 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 142 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9WTX5 |
jPOSTi | Q9WTX5 |
MaxQBi | Q9WTX5 |
PaxDbi | Q9WTX5 |
PRIDEi | Q9WTX5 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9WTX5 |
PTM databases
iPTMneti | Q9WTX5 |
PhosphoSitePlusi | Q9WTX5 |
SwissPalmi | Q9WTX5 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036309, Expressed in cochlea and 316 other tissues |
ExpressionAtlasi | Q9WTX5, baseline and differential |
Genevisiblei | Q9WTX5, MM |
Interactioni
Subunit structurei
Interacts with KDM2B, forming heterodimers (By similarity). The KDM2B-SKP1 heterodimeric complex interacts with the PCGF1-BCORL heterodimeric complex to form a homotetrameric polycomb repression complex 1 (PRC1.1) (By similarity).
Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes formed of CUL1, SKP1, RBX1 and a variable F-box domain-containing protein as substrate-specific subunit.
Component of the SCF(FBXW11) complex containing FBXW11.
Component of the SCF(SKP2) complex containing SKP2, in which it interacts directly with SKP1, SKP2 and RBX1.
Component of the SCF(FBXW2) complex containing FBXw2.
Component of the SCF(FBXO32) complex containing FBXO32.
Component of the probable SCF(FBXO7) complex containing FBXO7.
Component of the SCF(FBXO10) complex containing FBXO10.
Component of the SCF(FBXO11) complex containing FBXO11.
Component of the SCF(FBXO25) complex containing FBXO25.
Component of the SCF(FBXO33) complex containing FBXO33.
Component of the probable SCF(FBXO4) complex containing FBXO4.
Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44.
Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. This complex binds phosphorylated NFKBIA.
Part of a SCF complex consisting of CUL1, RBX1, SKP1 and FBXO2.
Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2.
Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17.
Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27.
Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF.
Component of the SCF(FBXL3) complex composed of CUL1, SKP1, RBX1 and FBXL3.
Component of the SCF(FBXL21) complex composed of CUL1, SKP1, RBX1 and FBXL21.
Component of the SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9.
Component of the SCF(FBXW7) composed of CUL1, SKP1, RBX1 and FBXW7.
Component of the SCF(FBXW15) complex containing FBXW15 (PubMed:23319590).
Interacts with CEP68 (By similarity).
Interacts with FBXW15 (PubMed:23319590).
Interacts with NOTCH2 (By similarity). The SKP1-KDM2A and SKP1-KDM2B complexes interact with UBB (By similarity).
By similarity6 PublicationsBinary interactionsi
Hide detailsQ9WTX5
With | #Exp. | IntAct |
---|---|---|
Fbxw5 [Q9QXW2] | 2 | EBI-1202363,EBI-16031930 |
Ppp1ca [P62137] | 2 | EBI-1202363,EBI-357187 |
Rnf2 [Q9CQJ4] | 4 | EBI-1202363,EBI-927321 |
FBXL3 [Q9UKT7] from Homo sapiens. | 4 | EBI-1202363,EBI-2557269 |
GO - Molecular functioni
- beta-catenin binding Source: MGI
- cullin family protein binding Source: MGI
- F-box domain binding Source: MGI
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203998, 102 interactors |
CORUMi | Q9WTX5 |
DIPi | DIP-38596N |
IntActi | Q9WTX5, 55 interactors |
MINTi | Q9WTX5 |
STRINGi | 10090.ENSMUSP00000038744 |
Miscellaneous databases
RNActi | Q9WTX5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 104 – 163 | Interaction with the F-box domain of F-box proteinsBy similarityAdd BLAST | 60 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1724, Eukaryota |
GeneTreei | ENSGT00390000012652 |
InParanoidi | Q9WTX5 |
OMAi | PLKSADM |
OrthoDBi | 1412723at2759 |
PhylomeDBi | Q9WTX5 |
TreeFami | TF354233 |
Family and domain databases
InterProi | View protein in InterPro IPR016897, SKP1 IPR001232, SKP1-like IPR036296, SKP1-like_dim_sf IPR011333, SKP1/BTB/POZ_sf IPR016072, Skp1_comp_dimer IPR016073, Skp1_comp_POZ |
Pfami | View protein in Pfam PF01466, Skp1, 1 hit PF03931, Skp1_POZ, 1 hit |
PIRSFi | PIRSF028729, E3_ubiquit_lig_SCF_Skp, 1 hit |
SMARTi | View protein in SMART SM00512, Skp1, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF81382, SSF81382, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPTIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV
60 70 80 90 100
NAAILKKVIQ WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL
110 120 130 140 150
FELILAANYL DIKGLLDVTC KTVANMIKGK TPEEIRKTFN IKNDFTEEEE
160
AQVRKENQWC EEK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PUV4 | E9PUV4_MOUSE | Cyclin-A/CDK2-associated protein p1... | Skp1 Skp1a | 96 | Annotation score: | ||
E9Q3Q7 | E9Q3Q7_MOUSE | S-phase kinase-associated protein 1 | Skp1 Skp1a | 56 | Annotation score: | ||
F6TGP8 | F6TGP8_MOUSE | S-phase kinase-associated protein 1 | Skp1 Skp1a | 50 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | D → N in BAC37220 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF083214 mRNA Translation: AAD16036.1 AK002983 mRNA Translation: BAB22496.1 AK010628 mRNA Translation: BAB27074.1 AK012498 mRNA Translation: BAB28281.1 AK014245 mRNA Translation: BAB29222.1 AK027909 mRNA Translation: BAC25660.1 AK078330 mRNA Translation: BAC37220.1 AK088339 mRNA Translation: BAC40292.1 BC002115 mRNA Translation: AAH02115.1 |
CCDSi | CCDS24669.1 |
RefSeqi | NP_035673.3, NM_011543.4 XP_006532849.1, XM_006532786.2 |
Genome annotation databases
Ensembli | ENSMUST00000037324; ENSMUSP00000038744; ENSMUSG00000036309 ENSMUST00000109072; ENSMUSP00000104700; ENSMUSG00000036309 |
GeneIDi | 21402 |
KEGGi | mmu:21402 |
UCSCi | uc007ivf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF083214 mRNA Translation: AAD16036.1 AK002983 mRNA Translation: BAB22496.1 AK010628 mRNA Translation: BAB27074.1 AK012498 mRNA Translation: BAB28281.1 AK014245 mRNA Translation: BAB29222.1 AK027909 mRNA Translation: BAC25660.1 AK078330 mRNA Translation: BAC37220.1 AK088339 mRNA Translation: BAC40292.1 BC002115 mRNA Translation: AAH02115.1 |
CCDSi | CCDS24669.1 |
RefSeqi | NP_035673.3, NM_011543.4 XP_006532849.1, XM_006532786.2 |
3D structure databases
SMRi | Q9WTX5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203998, 102 interactors |
CORUMi | Q9WTX5 |
DIPi | DIP-38596N |
IntActi | Q9WTX5, 55 interactors |
MINTi | Q9WTX5 |
STRINGi | 10090.ENSMUSP00000038744 |
PTM databases
iPTMneti | Q9WTX5 |
PhosphoSitePlusi | Q9WTX5 |
SwissPalmi | Q9WTX5 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9WTX5 |
Proteomic databases
EPDi | Q9WTX5 |
jPOSTi | Q9WTX5 |
MaxQBi | Q9WTX5 |
PaxDbi | Q9WTX5 |
PRIDEi | Q9WTX5 |
Protocols and materials databases
Antibodypediai | 4566, 526 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000037324; ENSMUSP00000038744; ENSMUSG00000036309 ENSMUST00000109072; ENSMUSP00000104700; ENSMUSG00000036309 |
GeneIDi | 21402 |
KEGGi | mmu:21402 |
UCSCi | uc007ivf.2, mouse |
Organism-specific databases
CTDi | 6500 |
MGIi | MGI:103575, Skp1 |
Phylogenomic databases
eggNOGi | KOG1724, Eukaryota |
GeneTreei | ENSGT00390000012652 |
InParanoidi | Q9WTX5 |
OMAi | PLKSADM |
OrthoDBi | 1412723at2759 |
PhylomeDBi | Q9WTX5 |
TreeFami | TF354233 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1170546, Prolactin receptor signaling R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5684264, MAP3K8 (TPL2)-dependent MAPK1/3 activation R-MMU-68949, Orc1 removal from chromatin R-MMU-69231, Cyclin D associated events in G1 R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-917937, Iron uptake and transport R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 21402, 6 hits in 16 CRISPR screens |
ChiTaRSi | Skp1a, mouse |
PROi | PR:Q9WTX5 |
RNActi | Q9WTX5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036309, Expressed in cochlea and 316 other tissues |
ExpressionAtlasi | Q9WTX5, baseline and differential |
Genevisiblei | Q9WTX5, MM |
Family and domain databases
InterProi | View protein in InterPro IPR016897, SKP1 IPR001232, SKP1-like IPR036296, SKP1-like_dim_sf IPR011333, SKP1/BTB/POZ_sf IPR016072, Skp1_comp_dimer IPR016073, Skp1_comp_POZ |
Pfami | View protein in Pfam PF01466, Skp1, 1 hit PF03931, Skp1_POZ, 1 hit |
PIRSFi | PIRSF028729, E3_ubiquit_lig_SCF_Skp, 1 hit |
SMARTi | View protein in SMART SM00512, Skp1, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF81382, SSF81382, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SKP1_MOUSE | |
Accessioni | Q9WTX5Primary (citable) accession number: Q9WTX5 Secondary accession number(s): Q8C5H6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families