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Protein

Interferon-inducible double-stranded RNA-dependent protein kinase activator A

Gene

Prkra

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 (By similarity). Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processRNA-mediated gene silencing

Enzyme and pathway databases

ReactomeiR-MMU-203927 MicroRNA (miRNA) biogenesis
R-MMU-426486 Small interfering RNA (siRNA) biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-inducible double-stranded RNA-dependent protein kinase activator A
Alternative name(s):
PKR-associated protein X
PKR-associating protein X
Short name:
RAX
Protein activator of the interferon-induced protein kinase
Protein kinase, interferon-inducible double-stranded RNA-dependent activator
Gene namesi
Name:Prkra
Synonyms:Rax
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1344375 Prkra

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002236101 – 313Interferon-inducible double-stranded RNA-dependent protein kinase activator AAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei246PhosphoserineBy similarity1
Modified residuei287PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9WTX2
MaxQBiQ9WTX2
PaxDbiQ9WTX2
PeptideAtlasiQ9WTX2
PRIDEiQ9WTX2

PTM databases

iPTMnetiQ9WTX2
PhosphoSitePlusiQ9WTX2

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, muscle, spleen and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000002731 Expressed in 265 organ(s), highest expression level in dorsal pancreas
CleanExiMM_RAX
GenevisibleiQ9WTX2 MM

Interactioni

Subunit structurei

Homodimer. Interacts with DICER1, AGO2 and TARBP2. Also able to interact with dsRNA (By similarity). Interacts with EIF2AK2/PKR through its DRBM domains. Interacts with DUS2L (via DRBM domain) (By similarity). Interacts with UBC9. Forms a complex with UBC9 and p53/TP53.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-1073 RISC-loading complex, PRKRA variant
IntActiQ9WTX2, 4 interactors
MINTiQ9WTX2
STRINGi10090.ENSMUSP00000002808

Structurei

3D structure databases

ProteinModelPortaliQ9WTX2
SMRiQ9WTX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 101DRBM 1PROSITE-ProRule annotationAdd BLAST68
Domaini126 – 194DRBM 2PROSITE-ProRule annotationAdd BLAST69
Domaini240 – 308DRBM 3PROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 103Sufficient for self-association and interaction with TARBP2By similarityAdd BLAST103
Regioni102 – 195Sufficient for self-association and interaction with TARBP2By similarityAdd BLAST94
Regioni195 – 313Sufficient for self-association and interaction with TARBP2By similarityAdd BLAST119

Domaini

Self-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3.By similarity

Sequence similaritiesi

Belongs to the PRKRA family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3732 Eukaryota
ENOG410XSCK LUCA
GeneTreeiENSGT00900000141030
HOGENOMiHOG000231919
HOVERGENiHBG001700
InParanoidiQ9WTX2
OMAiVTVCHGT
OrthoDBiEOG091G0I2L
PhylomeDBiQ9WTX2
TreeFamiTF315953

Family and domain databases

CDDicd00048 DSRM, 3 hits
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR033363 PRKRA
IPR032478 Staufen_C
PANTHERiPTHR10910:SF8 PTHR10910:SF8, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
PF16482 Staufen_C, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 3 hits
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 3 hits

Sequencei

Sequence statusi: Complete.

Q9WTX2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSHSRHRAEA PPLQREDSGT FSLGKMITAK PGKTPIQVLH EYGMKTKNIP
60 70 80 90 100
VYECERSDVQ VHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK
110 120 130 140 150
ANASICFAVP DPLMPDPSKQ PKNQLNPIGS LQELAIHHGW RLPEYTLSQE
160 170 180 190 200
GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA KFSNISPENH
210 220 230 240 250
ISLTNVVGHS LGCTWHSLRN SPGEKINLLK RSLLSLPNTD YIQLLSEIAS
260 270 280 290 300
EQGFNITYLD IEELSANGQY QCLAELSTSP ITVCHGSGIS CGNAQSDAAH
310
NALQYLKIIA ERK
Length:313
Mass (Da):34,371
Last modified:November 1, 1999 - v1
Checksum:i6EDF248A55A391D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177K → N in BAB28477 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083032 mRNA Translation: AAD33098.1
AK012798 mRNA Translation: BAB28477.1
AK155112 mRNA Translation: BAE33056.1
BC011311 mRNA Translation: AAH11311.1
CCDSiCCDS16159.1
RefSeqiNP_036001.1, NM_011871.2
UniGeneiMm.277250

Genome annotation databases

EnsembliENSMUST00000002808; ENSMUSP00000002808; ENSMUSG00000002731
GeneIDi23992
KEGGimmu:23992
UCSCiuc008kff.1 mouse

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083032 mRNA Translation: AAD33098.1
AK012798 mRNA Translation: BAB28477.1
AK155112 mRNA Translation: BAE33056.1
BC011311 mRNA Translation: AAH11311.1
CCDSiCCDS16159.1
RefSeqiNP_036001.1, NM_011871.2
UniGeneiMm.277250

3D structure databases

ProteinModelPortaliQ9WTX2
SMRiQ9WTX2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-1073 RISC-loading complex, PRKRA variant
IntActiQ9WTX2, 4 interactors
MINTiQ9WTX2
STRINGi10090.ENSMUSP00000002808

PTM databases

iPTMnetiQ9WTX2
PhosphoSitePlusiQ9WTX2

Proteomic databases

EPDiQ9WTX2
MaxQBiQ9WTX2
PaxDbiQ9WTX2
PeptideAtlasiQ9WTX2
PRIDEiQ9WTX2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002808; ENSMUSP00000002808; ENSMUSG00000002731
GeneIDi23992
KEGGimmu:23992
UCSCiuc008kff.1 mouse

Organism-specific databases

CTDi8575
MGIiMGI:1344375 Prkra

Phylogenomic databases

eggNOGiKOG3732 Eukaryota
ENOG410XSCK LUCA
GeneTreeiENSGT00900000141030
HOGENOMiHOG000231919
HOVERGENiHBG001700
InParanoidiQ9WTX2
OMAiVTVCHGT
OrthoDBiEOG091G0I2L
PhylomeDBiQ9WTX2
TreeFamiTF315953

Enzyme and pathway databases

ReactomeiR-MMU-203927 MicroRNA (miRNA) biogenesis
R-MMU-426486 Small interfering RNA (siRNA) biogenesis

Miscellaneous databases

PROiPR:Q9WTX2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002731 Expressed in 265 organ(s), highest expression level in dorsal pancreas
CleanExiMM_RAX
GenevisibleiQ9WTX2 MM

Family and domain databases

CDDicd00048 DSRM, 3 hits
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR033363 PRKRA
IPR032478 Staufen_C
PANTHERiPTHR10910:SF8 PTHR10910:SF8, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
PF16482 Staufen_C, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 3 hits
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPRKRA_MOUSE
AccessioniPrimary (citable) accession number: Q9WTX2
Secondary accession number(s): Q9CZB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 1, 1999
Last modified: September 12, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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