UniProtKB - Q9WTU6 (MK09_MOUSE)
Mitogen-activated protein kinase 9
Mapk9
Functioni
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (PubMed:29153991).
7 PublicationsCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 55 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 151 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 33 – 38 | ATPPROSITE-ProRule annotation | 6 |
GO - Molecular functioni
- ATP binding Source: MGI
- cysteine-type endopeptidase activator activity involved in apoptotic process Source: MGI
- JUN kinase activity Source: UniProtKB
- MAP kinase activity Source: GO_Central
- mitogen-activated protein kinase kinase kinase binding Source: MGI
- protein kinase activity Source: MGI
- protein serine/threonine/tyrosine kinase activity Source: UniProtKB
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cellular response to cadmium ion Source: MGI
- cellular response to reactive oxygen species Source: MGI
- cellular response to UV Source: MGI
- intracellular signal transduction Source: GO_Central
- JNK cascade Source: UniProtKB
- JUN phosphorylation Source: MGI
- neuron projection development Source: MGI
- peptidyl-serine phosphorylation Source: UniProtKB
- positive regulation of apoptotic process Source: MGI
- positive regulation of apoptotic signaling pathway Source: MGI
- positive regulation of cell morphogenesis involved in differentiation Source: MGI
- positive regulation of chemokine production Source: MGI
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of macrophage derived foam cell differentiation Source: MGI
- positive regulation of nitric oxide biosynthetic process Source: MGI
- positive regulation of nitric-oxide synthase biosynthetic process Source: MGI
- positive regulation of podosome assembly Source: MGI
- positive regulation of prostaglandin biosynthetic process Source: MGI
- positive regulation of prostaglandin secretion Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of protein ubiquitination Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription factor catabolic process Source: UniProtKB
- protein localization to tricellular tight junction Source: MGI
- protein phosphorylation Source: MGI
- regulation of circadian rhythm Source: UniProtKB
- regulation of JNK cascade Source: MGI
- regulation of protein ubiquitination Source: MGI
- release of cytochrome c from mitochondria Source: MGI
- response to cadmium ion Source: MGI
- response to xenobiotic stimulus Source: MGI
- rhythmic process Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Biological rhythms |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 3474 |
Reactomei | R-MMU-2559580, Oxidative Stress Induced Senescence R-MMU-2871796, FCERI mediated MAPK activation R-MMU-450321, JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 R-MMU-450341, Activation of the AP-1 family of transcription factors |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 9 (EC:2.7.11.245 Publications)Short name: MAP kinase 9 Short name: MAPK 9 Alternative name(s): Stress-activated protein kinase JNK2 c-Jun N-terminal kinase 2 |
Gene namesi | Name:Mapk9 Synonyms:Jnk2, Prkm9 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346862, Mapk9 |
VEuPathDBi | HostDB:ENSMUSG00000020366 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- neuron projection Source: MGI
- perikaryon Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186274 | 1 – 423 | Mitogen-activated protein kinase 9Add BLAST | 423 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 183 | Phosphothreonine; by MAP2K7Combined sources | 1 | |
Modified residuei | 185 | Phosphotyrosine; by MAP2K4Combined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9WTU6 |
MaxQBi | Q9WTU6 |
PaxDbi | Q9WTU6 |
PeptideAtlasi | Q9WTU6 |
PRIDEi | Q9WTU6 |
ProteomicsDBi | 295947 [Q9WTU6-1] 295948 [Q9WTU6-2] 295949 [Q9WTU6-3] 295950 [Q9WTU6-4] |
PTM databases
iPTMneti | Q9WTU6 |
PhosphoSitePlusi | Q9WTU6 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000020366, Expressed in secondary oocyte and 312 other tissues |
ExpressionAtlasi | Q9WTU6, baseline and differential |
Genevisiblei | Q9WTU6, MM |
Interactioni
Subunit structurei
Interacts with MECOM (By similarity). Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway (PubMed:11562351).
Interacts with NFATC4 (By similarity).
Interacts with ATF7; the interaction does not phosphorylate ATF7 but acts as a docking site for ATF7-associated partners such as JUN (By similarity).
Interacts with BCL10 (By similarity).
Interacts with CTNNB1 and GSK3B (By similarity).
Interacts with DCLK2 (PubMed:16628014).
Interacts with MAPKBP1 (By similarity).
Interacts with POU5F1; phosphorylates POU5F1 at 'Ser-347' (PubMed:29153991).
Found in a complex with SH3RF1, RAC2, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8IP1/JIP1 and MAPK8/JNK1 (PubMed:27084103).
By similarity4 PublicationsGO - Molecular functioni
Protein-protein interaction databases
BioGRIDi | 204972, 4 interactors |
DIPi | DIP-31076N |
ELMi | Q9WTU6 |
IntActi | Q9WTU6, 6 interactors |
MINTi | Q9WTU6 |
STRINGi | 10090.ENSMUSP00000020634 |
Chemistry databases
BindingDBi | Q9WTU6 |
Miscellaneous databases
RNActi | Q9WTU6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 321 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 296 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 366 – 423 | DisorderedSequence analysisAdd BLAST | 58 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 183 – 185 | TXY | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 374 – 415 | Polar residuesSequence analysisAdd BLAST | 42 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0665, Eukaryota |
GeneTreei | ENSGT00940000158327 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | Q9WTU6 |
OMAi | PSETEHD |
PhylomeDBi | Q9WTU6 |
TreeFami | TF105100 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008351, MAPK_JNK IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01772, JNKMAPKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSDSKSDGQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI
60 70 80 90 100
NVAVKKLSRP FQNQTHAKRA YRELVLLKCV NHKNIISLLN VFTPQKTLEE
110 120 130 140 150
FQDVYLVMEL MDANLCQVIH MELDHERMSY LLYQMLCGIK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTACTNF MMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYKENVDIWS VGCIMAEMVL HKVLFPGRDY IDQWNKVIEQ LGTPSAEFMK
260 270 280 290 300
KLQPTVRNYV ENRPKYPGIK FEELFPDWIF PSESERDKIK TSQARDLLSK
310 320 330 340 350
MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIE
360 370 380 390 400
EWKELIYKEV MDWEERSKNG VKDQPSDAAV SSKATPSQSS SINDISSMST
410 420
EHTLASDTDS SLDASTGPLE GCR
The sequence of this isoform differs from the canonical sequence as follows:
377-423: DAAVSSKATPSQSSSINDISSMSTEHTLASDTDSSLDASTGPLEGCR → AQMQQ
The sequence of this isoform differs from the canonical sequence as follows:
216-230: AEMVLHKVLFPGRDY → GELVKGCVIFQGTDH
377-423: DAAVSSKATPSQSSSINDISSMSTEHTLASDTDSSLDASTGPLEGCR → AQMQQ
The sequence of this isoform differs from the canonical sequence as follows:
216-230: AEMVLHKVLFPGRDY → GELVKGCVIFQGTDH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 223 | V → C (PubMed:10674476).Curated | 1 | |
Sequence conflicti | 237 | V → A in CAC88132 (PubMed:10674476).Curated | 1 | |
Sequence conflicti | 386 | P → A in BAC27623 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004836 | 216 – 230 | AEMVL…PGRDY → GELVKGCVIFQGTDH in isoform Beta-1 and isoform Beta-2. 3 PublicationsAdd BLAST | 15 | |
Alternative sequenceiVSP_004837 | 377 – 423 | DAAVS…LEGCR → AQMQQ in isoform Alpha-1 and isoform Beta-1. 2 PublicationsAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF052466 mRNA Translation: AAD22576.1 AF052467 mRNA Translation: AAD22577.1 AF052468 mRNA Translation: AAD22578.1 AF052469 mRNA Translation: AAD22579.1 AB005664 mRNA Translation: BAA85876.1 AJ315339 , AJ315340, AJ315341, AJ315342, AJ315343, AJ315344, AJ315345, AJ315346, AJ315347, AJ315348, AJ315349, AJ315350 Genomic DNA Translation: CAC88132.1 Sequence problems. AK031959 mRNA Translation: BAC27623.1 AL606479 Genomic DNA No translation available. BC028341 mRNA Translation: AAH28341.1 |
CCDSi | CCDS24623.1 [Q9WTU6-4] CCDS24624.1 [Q9WTU6-2] CCDS48782.1 [Q9WTU6-1] CCDS48783.1 [Q9WTU6-3] |
RefSeqi | NP_001157143.1, NM_001163671.1 [Q9WTU6-1] NP_001157144.1, NM_001163672.1 [Q9WTU6-3] NP_058657.1, NM_016961.3 [Q9WTU6-2] NP_997575.2, NM_207692.2 [Q9WTU6-4] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF052466 mRNA Translation: AAD22576.1 AF052467 mRNA Translation: AAD22577.1 AF052468 mRNA Translation: AAD22578.1 AF052469 mRNA Translation: AAD22579.1 AB005664 mRNA Translation: BAA85876.1 AJ315339 , AJ315340, AJ315341, AJ315342, AJ315343, AJ315344, AJ315345, AJ315346, AJ315347, AJ315348, AJ315349, AJ315350 Genomic DNA Translation: CAC88132.1 Sequence problems. AK031959 mRNA Translation: BAC27623.1 AL606479 Genomic DNA No translation available. BC028341 mRNA Translation: AAH28341.1 |
CCDSi | CCDS24623.1 [Q9WTU6-4] CCDS24624.1 [Q9WTU6-2] CCDS48782.1 [Q9WTU6-1] CCDS48783.1 [Q9WTU6-3] |
RefSeqi | NP_001157143.1, NM_001163671.1 [Q9WTU6-1] NP_001157144.1, NM_001163672.1 [Q9WTU6-3] NP_058657.1, NM_016961.3 [Q9WTU6-2] NP_997575.2, NM_207692.2 [Q9WTU6-4] |
3D structure databases
SMRi | Q9WTU6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204972, 4 interactors |
DIPi | DIP-31076N |
ELMi | Q9WTU6 |
IntActi | Q9WTU6, 6 interactors |
MINTi | Q9WTU6 |
STRINGi | 10090.ENSMUSP00000020634 |
Chemistry databases
BindingDBi | Q9WTU6 |
ChEMBLi | CHEMBL2034797 |
PTM databases
iPTMneti | Q9WTU6 |
PhosphoSitePlusi | Q9WTU6 |
Proteomic databases
jPOSTi | Q9WTU6 |
MaxQBi | Q9WTU6 |
PaxDbi | Q9WTU6 |
PeptideAtlasi | Q9WTU6 |
PRIDEi | Q9WTU6 |
ProteomicsDBi | 295947 [Q9WTU6-1] 295948 [Q9WTU6-2] 295949 [Q9WTU6-3] 295950 [Q9WTU6-4] |
Protocols and materials databases
Antibodypediai | 4557, 891 antibodies from 47 providers |
DNASUi | 26420 |
Genome annotation databases
Organism-specific databases
CTDi | 5601 |
MGIi | MGI:1346862, Mapk9 |
VEuPathDBi | HostDB:ENSMUSG00000020366 |
Phylogenomic databases
eggNOGi | KOG0665, Eukaryota |
GeneTreei | ENSGT00940000158327 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | Q9WTU6 |
OMAi | PSETEHD |
PhylomeDBi | Q9WTU6 |
TreeFami | TF105100 |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 3474 |
Reactomei | R-MMU-2559580, Oxidative Stress Induced Senescence R-MMU-2871796, FCERI mediated MAPK activation R-MMU-450321, JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 R-MMU-450341, Activation of the AP-1 family of transcription factors |
Miscellaneous databases
BioGRID-ORCSi | 26420, 2 hits in 64 CRISPR screens |
ChiTaRSi | Mapk9, mouse |
PROi | PR:Q9WTU6 |
RNActi | Q9WTU6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020366, Expressed in secondary oocyte and 312 other tissues |
ExpressionAtlasi | Q9WTU6, baseline and differential |
Genevisiblei | Q9WTU6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008351, MAPK_JNK IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01772, JNKMAPKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MK09_MOUSE | |
Accessioni | Q9WTU6Primary (citable) accession number: Q9WTU6 Secondary accession number(s): Q5NCK9 Q9WTU5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | February 21, 2001 | |
Last modified: | February 23, 2022 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families