UniProtKB - Q9WTR2 (M3K6_MOUSE)
Protein
Mitogen-activated protein kinase kinase kinase 6
Gene
Map3k6
Organism
Mus musculus (Mouse)
Status
Functioni
Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways.1 Publication
Catalytic activityi
Cofactori
Mg2+1 Publication
Activity regulationi
Activated by phosphorylation on Thr-807. Catalytically active only when complexed with MAP3K5, with MAP3K5 supporting the stability and the active configuration of MAP3K6 and MAP3K6 activating MAP3K5 by direct phosphorylation.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 678 | ATPPROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 772 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 655 – 663 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: MGI
- magnesium ion binding Source: MGI
- MAP kinase kinase kinase activity Source: MGI
- protein kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
- protein threonine kinase activity Source: RHEA
GO - Biological processi
- activation of MAPKK activity Source: GO_Central
- protein phosphorylation Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.25, 3474 |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase kinase kinase 6 (EC:2.7.11.25)Alternative name(s): Apoptosis signal-regulating kinase 2 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1855691, Map3k6 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 678 | K → M: Loss of kinase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000291648 | 1 – 1291 | Mitogen-activated protein kinase kinase kinase 6Add BLAST | 1291 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 807 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 965 | PhosphoserineBy similarity | 1 | |
Modified residuei | 985 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q9WTR2 |
PaxDbi | Q9WTR2 |
PRIDEi | Q9WTR2 |
ProteomicsDBi | 292139 |
PTM databases
iPTMneti | Q9WTR2 |
PhosphoSitePlusi | Q9WTR2 |
Interactioni
Subunit structurei
Binds both upstream activators and downstream substrates in multimolecular complexes.
1 PublicationBinary interactionsi
Q9WTR2
With | #Exp. | IntAct |
---|---|---|
MAP3K5 [Q99683] from Homo sapiens. | 3 | EBI-1254790,EBI-476263 |
Protein-protein interaction databases
IntActi | Q9WTR2, 2 interactors |
STRINGi | 10090.ENSMUSP00000030677 |
Miscellaneous databases
RNActi | Q9WTR2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 649 – 907 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 259 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 1006 – 1069 | Sequence analysisAdd BLAST | 64 | |
Coiled coili | 1164 – 1195 | Sequence analysisAdd BLAST | 32 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4279, Eukaryota |
InParanoidi | Q9WTR2 |
PhylomeDBi | Q9WTR2 |
TreeFami | TF105115 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR043969, MAP3K_PH IPR025136, MAP3K_TRAF-bd IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR013761, SAM/pointed_sf IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF19039, ASK_PH, 1 hit PF13281, DUF4071, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9WTR2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGPCPRAGV LERAGSCWQD PLAEALSRGR SSPAVTGRGC ARSRPLSVVY
60 70 80 90 100
VLTREPGPGV EPGSGTEAEP LPLRCLREAC AQLQGTRPPP QLRSLPFATL
110 120 130 140 150
ALGDTAALDS FYNADVVVLE VSSSLAQPSL FYHLGVRESF SMTNNVLLCS
160 170 180 190 200
QAELPDLQAL REDVFQKNSD CVGSYTLIPY VVTATGRVLC GDAGLLRGIA
210 220 230 240 250
DGLVQAGAGT EALLTPLVGR LVRLLEATPT DSCGYFRETI RQDIRQARER
260 270 280 290 300
FSGQQLRQEL ARLQRRLDSV ELLSPDIVMN LLLSYRDVQD YSAIIELVET
310 320 330 340 350
LQALPTCDVA EQHNVCFHYT FALNRRNRPG DREKALAVLL PLVKYEGPVA
360 370 380 390 400
PDLYCMCGRI YKDMFFTSGF QNAGHLEQAY HWYRKAFDVE PSLHSGINAA
410 420 430 440 450
VLLIAAGQHF EDSEELRLIG MKLACLLARK GCVEKMQYYW DVGFYLGAQI
460 470 480 490 500
LANDPIQVVL AAEQLYKLNA PIWYLVSVME TFLLYQHFRP TPEPSGGPPL
510 520 530 540 550
RAHFWLHFLL QSCQPFKMAP PQEDQCLVLV LEINKVLLPA RLEIQGTDPM
560 570 580 590 600
SAVTLSLLEP ETQEDPSSWT FPVTSICGIS TSKLDQRCCF LYALPPAQDV
610 620 630 640 650
QLCFPSVERC QRFCGLIQVL VMNPDSSAPT EEAEGAREVL EFDYEYSETG
660 670 680 690 700
ERLVLGRGTY GVVYAGRDRH TRVRIAIKEI PERDSRFSQP LHEEIALHKR
710 720 730 740 750
LRHKNIVRYL GSASQGGYLK IFMEEVPGGS LSSLLRSVWG PLKDNESTIS
760 770 780 790 800
FYTRQILQGL SYLHENRIVH RDIKGDNVLI NTFSGLLKIS DFGTSKRLAG
810 820 830 840 850
ITPCTETFTG TLQYMAPEII DQGPRGYGKA ADIWSLGCTV IEMATGRPPF
860 870 880 890 900
HELGSPQAAM FQVGMYKVHP PVPGSLSAEA QAFLLRTFEP DPRLRASAQE
910 920 930 940 950
LLGDPFLQPG KRSRSPGSPR HTPRPSGAPS GPSSPSADSA TQSQTFPRPQ
960 970 980 990 1000
APSQHPPSPP KRCLSYGDTS QLRVPEEPAA EEPASPEESS GLSLLHQESK
1010 1020 1030 1040 1050
RRAMLAAVLE QEVPTLAENL LEQEQDSRLS KIHVELLLRC LGAQIHTPNR
1060 1070 1080 1090 1100
RQLAQELRTL QAQLRAQSLG PALLKGPLFA FPDAVKQILR RRQIRPHWMF
1110 1120 1130 1140 1150
VLDSLLSRAV RAALAVLDAE SEKKAVLPRS EESSKEESQQ KPQESQALQS
1160 1170 1180 1190 1200
QLPPEQGPPS LMVELGLLRA ETDRLRDLLA EKERECQALV QQALHRVHAE
1210 1220 1230 1240 1250
TRKYAPASET PATLPKDQNL VRWLQELSVD PATIQTLLSH SFTLQTLLTC
1260 1270 1280 1290
ATQDDLVYTR IRGGMVCRIW RAILAQRAGA TSVTPVPRDA E
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MQ82 | A0A0A0MQ82_MOUSE | Mitogen-activated protein kinase ki... | Map3k6 | 1,291 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 7 | R → G in CAM21067 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 7 | R → G in CAM18232 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 58 | P → L in CAM21067 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 58 | P → L in CAM18232 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 550 – 552 | MSA → VST in CAM21067 (PubMed:19468303).Curated | 3 | |
Sequence conflicti | 550 – 552 | MSA → VST in CAM18232 (PubMed:19468303).Curated | 3 | |
Sequence conflicti | 559 | E → D in CAM21067 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 559 | E → D in CAM18232 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 1114 | L → M in CAM21067 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 1114 | L → M in CAM18232 (PubMed:19468303).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB021861 mRNA Translation: BAA78532.4 AL671882, AL837525 Genomic DNA Translation: CAM21067.1 AL837525, AL671882 Genomic DNA Translation: CAM18232.1 BC120565 mRNA Translation: AAI20566.1 BC125577 mRNA Translation: AAI25578.1 |
CCDSi | CCDS18744.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB021861 mRNA Translation: BAA78532.4 AL671882, AL837525 Genomic DNA Translation: CAM21067.1 AL837525, AL671882 Genomic DNA Translation: CAM18232.1 BC120565 mRNA Translation: AAI20566.1 BC125577 mRNA Translation: AAI25578.1 |
CCDSi | CCDS18744.1 |
3D structure databases
SMRi | Q9WTR2 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9WTR2, 2 interactors |
STRINGi | 10090.ENSMUSP00000030677 |
PTM databases
iPTMneti | Q9WTR2 |
PhosphoSitePlusi | Q9WTR2 |
Proteomic databases
MaxQBi | Q9WTR2 |
PaxDbi | Q9WTR2 |
PRIDEi | Q9WTR2 |
ProteomicsDBi | 292139 |
Organism-specific databases
MGIi | MGI:1855691, Map3k6 |
Phylogenomic databases
eggNOGi | KOG4279, Eukaryota |
InParanoidi | Q9WTR2 |
PhylomeDBi | Q9WTR2 |
TreeFami | TF105115 |
Enzyme and pathway databases
BRENDAi | 2.7.11.25, 3474 |
Miscellaneous databases
ChiTaRSi | Map3k6, mouse |
PROi | PR:Q9WTR2 |
RNActi | Q9WTR2, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR043969, MAP3K_PH IPR025136, MAP3K_TRAF-bd IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR013761, SAM/pointed_sf IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF19039, ASK_PH, 1 hit PF13281, DUF4071, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | M3K6_MOUSE | |
Accessioni | Q9WTR2Primary (citable) accession number: Q9WTR2 Secondary accession number(s): A2AE93, Q0VBN3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2007 |
Last sequence update: | June 26, 2007 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families